Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 310,392 T→G 8.3% T378P (ACC→CCC)  RCAP_rcc00272 ← heparinase II/III family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034310,3920T→G 8.3% 7.0 ‑2.5 0.0132T378P (ACC→CCC) RCAP_rcc00272heparinase II/III family protein
Reads supporting (aligned to +/- strand):  new base (9/4):  ref base (51/67):  total (61/71)
Fisher's exact test strand distribution p-value = 8.60e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GTTTCCTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCAGC  >  NC_014034/310292‑310489
                                                                                                    |                                                                                                 
gTTTCCCCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGt                                                                                                   <  2:1151483/101‑1 (MQ=255)
 ttcccccGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTg                                                                                                  <  2:1971545/95‑1 (MQ=255)
 ttcccTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTg                                                                                                  <  1:2486250/98‑1 (MQ=255)
    ccTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGgcg                                                                                               <  1:1368037/101‑1 (MQ=255)
    ccTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGgcg                                                                                               <  1:596912/101‑1 (MQ=255)
    ccTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAACCGCGACGAGGAAAAACCCCCGATCGCCAGCGTCGAATGGCTGGGGGGGgcg                                                                                               >  2:2229135/1‑101 (MQ=255)
     cTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGGGGGGCCAAGCCCCGAGGAGAGAAAACCCCCGATCGCGGGCGTAGACTGGCCGGGGGGggggg                                                                                              >  2:1726259/1‑98 (MQ=255)
      tCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGggcg                                                                                             >  1:960074/1‑101 (MQ=255)
      ccGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGcgcg                                                                                             <  1:1898694/100‑1 (MQ=255)
        gAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAAACCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGGGcggc                                                                                           >  1:779500/1‑101 (MQ=255)
         aGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCCCGATCGCCCGCGTCGAATGGCTGGGGGGGGGGcggcc                                                                                          >  1:2975332/1‑101 (MQ=255)
           ccAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGcggccgg                                                                                        <  1:1889782/101‑1 (MQ=255)
             aGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGGGCGGGCGgcg                                                                                      >  2:3641164/1‑101 (MQ=255)
             aGGTCCGCATCCCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGgcg                                                                                      <  1:4208549/101‑1 (MQ=255)
              ggTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGgcgc                                                                                     <  2:1214553/101‑1 (MQ=255)
               gTCCGAATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGcgcg                                                                                    <  1:1743214/101‑1 (MQ=255)
                  cGCATCGCCGCGCCGGTGCGGCGGGGGGGGGCAAATCGCGACGAGGGAAAACCCCCGACCTCCACCGGCGAATTGCCGGGGGGTGCGcggccgggccgggg                                                                                 >  2:1672167/1‑94 (MQ=255)
                   gCATCGCCGCGCCGGTGCGGCGGGAGGGGCCAAACCGCCACGAGGAATAACCCTCGATCGCCAGCGGCGAATGGCTGGGGGGGGCGCGGCCGGCGCGGGGc                                                                                >  2:2223270/1‑101 (MQ=255)
                    cATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCCCGACGAGGAATAACCCTCGATCGCCCGCGTCGAATGGCTGGGGGGGGGGCGGCCCGCGCGGGGcc                                                                               >  1:301280/1‑101 (MQ=255)
                      tCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAAACCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGGGCGGGCCGCGCGNNGnccg                                                                             >  1:1652960/1‑99 (MQ=255)
                       cgccgcgccgGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAAACCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGGGCGGCCGGCGCGGGGcccgg                                                                            >  1:3182572/1‑98 (MQ=255)
                         ccgcgccgGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGCGCGGCCGGCGCGGGGCCACTCg                                                                          >  2:2548690/1‑101 (MQ=255)
                          cgcgccgGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGGGCGGCCGGCGCGGGGCCCGGCgg                                                                         >  1:2865362/1‑101 (MQ=255)
                          cgcgccgGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGCGCGGCCGGCGCGGGGCCCGCCgg                                                                         >  1:3789302/1‑101 (MQ=255)
                           gcgccgGGGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCggg                                                                        <  1:867811/101‑1 (MQ=255)
                            cgccgGTGCGGCGGGCGGGCCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCgggg                                                                       <  1:2262866/101‑1 (MQ=255)
                              ccgGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGcc                                                                     <  2:2166605/101‑1 (MQ=255)
                                ggTGCGGCGGGCGGGGCCAAGCCTCGGCGGGGAATAACCCTTGATCGCCAGCGTCGAAGGGCTGGGGGGGGTGGGGGCGGGGGGGGGGCAGTCGGGGGCGa                                                                   >  2:700008/1‑101 (MQ=255)
                                ggTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGCCGCGGTGCCAGTCGGGGCCGa                                                                   <  1:2407607/101‑1 (MQ=255)
                                ggTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGGGCGGCCGGCGCGGGGCCAGGCGGGGGCgg                                                                   >  1:3659121/1‑100 (MQ=255)
                                  tGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAAc                                                                 <  1:1102270/101‑1 (MQ=255)
                                     ggcgggcgGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATc                                                              <  2:913525/101‑1 (MQ=255)
                                     ggcgggcgGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATc                                                              <  2:3627153/101‑1 (MQ=255)
                                       ggggcgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGc                                                            <  1:2070940/100‑1 (MQ=255)
                                       cgggcgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGc                                                            >  2:2458326/1‑101 (MQ=255)
                                       cgggcgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGc                                                            <  2:3193397/101‑1 (MQ=255)
                                        gggcgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGcc                                                           >  2:4102641/1‑101 (MQ=255)
                                           ggggcCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGccccc                                                        <  2:1060439/100‑1 (MQ=255)
                                           cgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGccccc                                                        <  1:743710/101‑1 (MQ=255)
                                           cgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGccccc                                                        <  1:2199825/101‑1 (MQ=255)
                                           cgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGGGCCAGTCGGGGCCGAACATCGccccc                                                        >  2:2607257/1‑101 (MQ=255)
                                            ggggCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCg                                                       <  1:3895119/101‑1 (MQ=255)
                                              ggCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAg                                                     <  1:2149078/101‑1 (MQ=255)
                                               gCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGc                                                    <  1:924706/101‑1 (MQ=255)
                                                 cAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGGGCCAGTCGGGGCCGAACATCGCCCCCGAGCCg                                                  >  1:3475725/1‑101 (MQ=255)
                                                  aaGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGAGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGc                                                 >  1:4190167/1‑101 (MQ=255)
                                                   aGCCGCGACGAGGAATACCCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCa                                                <  2:3877025/101‑1 (MQ=255)
                                                   aGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCa                                                <  2:3789045/101‑1 (MQ=255)
                                                    gCCGCGACGAGGAATACCCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCaa                                               <  1:1272011/101‑1 (MQ=255)
                                                     ccGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAAt                                              >  2:1630794/1‑101 (MQ=255)
                                                      cgcgACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAAtt                                             <  1:2720473/101‑1 (MQ=255)
                                                         gacgaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGAc                                          >  2:2664447/1‑101 (MQ=255)
                                                           cgaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGGACATCGCCCCCGAACCGCAATCTACGa                                        >  2:4100340/1‑101 (MQ=255)
                                                            gaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGAt                                       >  1:2824242/1‑101 (MQ=255)
                                                            gaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGAt                                       >  2:1523679/1‑101 (MQ=255)
                                                             aGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATc                                      >  2:2734684/1‑101 (MQ=255)
                                                              ggAATACCCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCa                                     <  1:1572492/101‑1 (MQ=255)
                                                              ggAATACCCCTCGATCGCCAGCGGCGCATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCa                                     <  1:2548881/101‑1 (MQ=255)
                                                              ggAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCa                                     <  2:1765576/101‑1 (MQ=255)
                                                               gAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAg                                    <  2:2978897/101‑1 (MQ=255)
                                                                aaTACCCCTCGATCGCCAGCGTCGAAGGGCGGGGGGGGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGc                                   <  1:1819510/101‑1 (MQ=255)
                                                                aaTACCCCTCGACCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGc                                   <  1:500853/101‑1 (MQ=255)
                                                                aaTAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGTGCCGCAATTGACGATCAGc                                   >  2:2577456/1‑101 (MQ=255)
                                                                aaTAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGc                                   <  1:1841911/101‑1 (MQ=255)
                                                                  tAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCgg                                 >  2:4216472/1‑101 (MQ=255)
                                                                  tAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCgg                                 <  1:1053969/101‑1 (MQ=255)
                                                                   accccTCGATCGCCAGCGTCGAATGGGTGGGGGGGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGc                                <  1:4146523/99‑1 (MQ=255)
                                                                   aaCCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGc                                <  2:3440924/101‑1 (MQ=255)
                                                                     cccTGGATCGCAACCGTGGAATGGGGGGGGGTGGCGCGGCGGGCGCGGTGCCAGTGGGGGCCAAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCa                              <  1:3275874/101‑1 (MQ=255)
                                                                      ccTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAg                             <  1:1168641/101‑1 (MQ=255)
                                                                       cccGATCGCCAGGGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCCCCCCCGAGCCGCAATTGACGATCAGCGGCCAgc                            <  2:4201357/99‑1 (MQ=255)
                                                                       cTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAgc                            >  1:1472978/1‑101 (MQ=255)
                                                                       cTCGACCGCCAGCGTCGAATGGCTGGGGGGNGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAgc                            <  1:616275/101‑1 (MQ=255)
                                                                        tCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAgcg                           >  1:2284006/1‑101 (MQ=255)
                                                                        tCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGAGCGGTGCCAGCCGGGGCCGCACATCGGCCCCGAGCCGCAATGGACGATCAGCGGcccccg                           >  1:1368217/1‑97 (MQ=255)
                                                                            tccccAGCGTCGAAGGGCTGGGGGTGGCGCGGCGGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGcccc                       <  2:955127/98‑1 (MQ=255)
                                                                            tCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGcccc                       >  2:3276162/1‑101 (MQ=255)
                                                                            tCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGcccc                       >  1:3966724/1‑101 (MQ=255)
                                                                             cGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCg                      >  2:3928986/1‑101 (MQ=255)
                                                                               ccagggaggAATGGGTGGGGGGGGCGCGGCCGGCGGGGTGCCAGGCGGGGCCAAACATCCCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAg                    <  2:2049477/93‑1 (MQ=255)
                                                                               ccAGCGTCGAAGGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAg                    <  2:3136356/101‑1 (MQ=255)
                                                                                cAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAgg                   >  1:3494389/1‑101 (MQ=255)
                                                                                 aGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCCGCGCCCCGAggg                  >  1:1018128/1‑100 (MQ=255)
                                                                                  gCGTCGAATGGCGGGGGGGGGCGCGGCCGGCGCGGTCCCAGTCGGGGCCGAACACCGCCCCGGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTg                 <  2:1453877/101‑1 (MQ=255)
                                                                                  gCGGCGAGTGGGTGGGGGTGGCGCGCCCGGGGGTTTGCCAGTCGGGGTCAAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTg                 <  1:3904267/101‑1 (MQ=255)
                                                                                    gTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAg               >  2:2415309/1‑101 (MQ=255)
                                                                                     tggAATGGCGGGGGGGGGGGCGGCCGGGGGTGGGCCAGGGGGGGCCGACCCGCGCCCCGGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGt              <  1:1672282/99‑1 (MQ=255)
                                                                                      cGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGGGAGtt             >  2:564943/1‑101 (MQ=255)
                                                                                      cGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGCGAGtt             >  2:777137/1‑101 (MQ=255)
                                                                                       gagtGGCGGGGGGGGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCGGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTc            <  2:2865143/98‑1 (MQ=255)
                                                                                       gAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTc            <  1:2651543/101‑1 (MQ=255)
                                                                                       gAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTc            >  2:3223458/1‑101 (MQ=255)
                                                                                       gAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTc            >  2:288120/1‑101 (MQ=255)
                                                                                       gAAGGGCGGGCGGGGCCGCGCCCGGCGCGGTGCCAGTCGGGGCCGAACATCCCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTc            <  1:1879213/101‑1 (MQ=255)
                                                                                        attGGCTGGGGGTGGCGGGGCCGGCGGGGTGCCAGTGGGGGCCGAACATCCCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCa           <  2:3475473/99‑1 (MQ=255)
                                                                                         aTGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCaa          >  2:1451580/1‑101 (MQ=255)
                                                                                          tGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCaaa         <  1:2458510/101‑1 (MQ=255)
                                                                                             cTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGc      >  2:2773941/1‑101 (MQ=255)
                                                                                               gggggTGGCGCGGCCGGCGGGGGCCCGGTCGGGGCCAAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCa    <  2:3709160/101‑1 (MQ=255)
                                                                                                ggggGGGCGCGGCGGGCGCGGTCCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCAg   <  2:3065381/101‑1 (MQ=255)
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GTTTCCTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCAGC  >  NC_014034/310292‑310489

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG