Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 317,927 A→G 100% K338E (AAG→GAG)  RCAP_rcc00280 → hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034317,9270A→G100.0% 875.4 230K338E (AAG→GAG) RCAP_rcc00280hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (107/123):  ref base (0/0):  total (107/123)

Only 100 of 233 total aligned reads displayed.

AAGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317829‑318024
                                                                                                   |                                                                                                 
aaGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAg                                                                                                 <  1:3589484/101‑1 (MQ=255)
aaGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAg                                                                                                 <  1:119560/101‑1 (MQ=255)
      cGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGAtgtg                                                                                           >  2:1027179/1‑101 (MQ=255)
      cGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGAtgtg                                                                                           >  1:1813870/1‑101 (MQ=255)
         tGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctc                                                                                        >  2:3024470/1‑101 (MQ=255)
           gAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCtt                                                                                      >  2:2607047/1‑101 (MQ=255)
            aGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTg                                                                                     <  2:606020/101‑1 (MQ=255)
            aGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTg                                                                                     <  2:3108558/101‑1 (MQ=255)
              cccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGAt                                                                                   <  1:177806/101‑1 (MQ=255)
               ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  2:1017611/101‑1 (MQ=255)
                cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 <  2:1305474/101‑1 (MQ=255)
                cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 <  2:1660538/101‑1 (MQ=255)
                cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 <  1:1546862/101‑1 (MQ=255)
                cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 >  2:1790922/1‑101 (MQ=255)
                 gcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCAc                                                                                <  1:3407076/101‑1 (MQ=255)
                 gcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCAc                                                                                <  2:2040622/101‑1 (MQ=255)
                 gcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCAc                                                                                <  2:669185/101‑1 (MQ=255)
                 gcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCACCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATcgc                                                                                <  2:3475917/101‑3 (MQ=255)
                  cgacgaCCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACg                                                                               <  2:2805078/101‑1 (MQ=255)
                   gacgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGc                                                                              >  2:3607087/1‑101 (MQ=255)
                     cgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGccc                                                                            <  2:284432/101‑1 (MQ=255)
                      gacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCg                                                                           >  1:2084427/1‑101 (MQ=255)
                        ccGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAg                                                                         >  1:2108467/1‑101 (MQ=255)
                         cGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAgg                                                                        >  1:278474/1‑101 (MQ=255)
                          gAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGa                                                                       <  1:3137986/101‑1 (MQ=255)
                            gggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAt                                                                     >  1:3523576/1‑101 (MQ=255)
                            gggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAt                                                                     >  2:2507334/1‑101 (MQ=255)
                             ggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAtt                                                                    <  1:468423/101‑1 (MQ=255)
                               cAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCg                                                                  <  1:1277812/101‑1 (MQ=255)
                               cAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCg                                                                  >  1:3201858/1‑101 (MQ=255)
                                 aTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgc                                                                <  2:2340213/101‑1 (MQ=255)
                                  tGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcg                                                               <  1:1685198/101‑1 (MQ=255)
                                   gTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcgc                                                              >  1:2680092/1‑101 (MQ=255)
                                    tCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGcc                                                             <  1:2435176/101‑1 (MQ=255)
                                    tCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGcc                                                             >  1:112286/1‑101 (MQ=255)
                                        gATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         >  2:1742166/1‑101 (MQ=255)
                                        gATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         <  2:1607611/101‑1 (MQ=255)
                                        gATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         <  1:1023584/101‑1 (MQ=255)
                                         aacccgatGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTg                                                        >  2:1504797/8‑101 (MQ=255)
                                         aTCACGCTGAACGGGGTGCATCTGGCCGAGGGG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTg                                                        >  2:1379812/1‑101 (MQ=255)
                                           cACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAc                                                      <  2:3665897/101‑1 (MQ=255)
                                              gCTGAACGGGGTGCATCTGGCCGGGGTGTGTC.ATGCGGGCGATCTGCGCCACGAGGATGTGCTCTTGAGCACGCCCGAGGAATTCGGCGCTATCTGTCCgg                                                   <  2:429507/101‑1 (MQ=255)
                                              gCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCgg                                                   <  1:2973317/101‑1 (MQ=255)
                                               cTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCggg                                                  <  2:3644126/101‑1 (MQ=255)
                                                 gAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGcc                                                >  2:2357794/1‑101 (MQ=255)
                                                  aaCGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               >  1:617204/1‑101 (MQ=255)
                                                   aCGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCCCGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTg                                              <  1:1030460/101‑1 (MQ=255)
                                                   aCGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTg                                              <  1:3033202/101‑1 (MQ=255)
                                                    cGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgg                                             >  1:4101939/1‑101 (MQ=255)
                                                     ggggTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTggg                                            >  2:907305/1‑101 (MQ=255)
                                                        gTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCt                                         <  2:2928128/101‑1 (MQ=255)
                                                        gTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCt                                         <  1:937227/101‑1 (MQ=255)
                                                           cATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCCCCCCGAGGATGTGCTCTTGATGACGCCCGAGGAATTCGGCGCCATCTGAGCGGGCCGGGGGCTTcc                                      >  1:2880389/1‑101 (MQ=255)
                                                            aTCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTccc                                     <  1:3482909/101‑1 (MQ=255)
                                                            aTCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTccc                                     <  2:112262/101‑1 (MQ=255)
                                                              cTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCtt                                   >  2:4191866/1‑101 (MQ=255)
                                                              cTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCtt                                   <  2:246963/101‑1 (MQ=255)
                                                               tGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  >  2:4084366/1‑101 (MQ=255)
                                                               tGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  2:1177161/101‑1 (MQ=255)
                                                               tGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttg                                  >  1:1413082/1‑100 (MQ=255)
                                                               gggCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCTGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  1:3635367/100‑1 (MQ=255)
                                                                ggCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTg                                 >  2:3492287/1‑101 (MQ=255)
                                                                ggCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTg                                 >  1:1066040/1‑101 (MQ=255)
                                                                 gCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGc                                >  2:3763330/1‑101 (MQ=255)
                                                                 gCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGc                                <  1:3069586/101‑1 (MQ=255)
                                                                 gCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGc                                <  1:3284097/101‑1 (MQ=255)
                                                                  ccGAGGTG.GTCGATGCGGGAGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcc                               >  2:865831/1‑101 (MQ=255)
                                                                   cGAGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGccc                              <  2:2438606/101‑1 (MQ=255)
                                                                     aGGTG.GTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCg                            <  1:2593963/101‑1 (MQ=255)
                                                                       gtg.gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCt                          <  1:198969/101‑1 (MQ=255)
                                                                       gtg.gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCt                          >  1:3313301/1‑101 (MQ=255)
                                                                        tg.gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtt                         >  1:747486/1‑101 (MQ=255)
                                                                        tg.gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtt                         <  2:3847267/101‑1 (MQ=255)
                                                                        tg.gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtt                         <  2:814349/101‑1 (MQ=255)
                                                                         g.gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCttt                        >  1:3562481/1‑101 (MQ=255)
                                                                         g.gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCttt                        >  1:3832089/1‑101 (MQ=255)
                                                                              gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    >  2:76052/1‑101 (MQ=255)
                                                                              gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    >  2:3248118/1‑101 (MQ=255)
                                                                               aTGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGc                   >  1:3252222/1‑101 (MQ=255)
                                                                               aTGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGc                   <  1:4084627/101‑1 (MQ=255)
                                                                                tGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCt                  >  2:2389481/1‑101 (MQ=255)
                                                                                 gCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTa                 <  1:2518872/101‑1 (MQ=255)
                                                                                 gCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTa                 <  2:3193018/101‑1 (MQ=255)
                                                                                 gCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTa                 >  1:1516099/1‑101 (MQ=255)
                                                                                  cGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaa                <  1:1871259/101‑1 (MQ=255)
                                                                                   gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               <  1:2644079/101‑1 (MQ=255)
                                                                                   gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               >  2:2918580/1‑101 (MQ=255)
                                                                                   gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               >  2:3334628/1‑101 (MQ=255)
                                                                                    ggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAc              <  2:2859977/101‑1 (MQ=255)
                                                                                     gCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcg             <  2:3718533/101‑1 (MQ=255)
                                                                                       gATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgcc           >  2:1984927/1‑101 (MQ=255)
                                                                                        aTCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccg          >  1:1632136/1‑101 (MQ=255)
                                                                                        aTCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccg          >  1:22658/1‑101 (MQ=255)
                                                                                        aTCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccg          <  1:1335614/101‑1 (MQ=255)
                                                                                         tCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccgc         <  2:3968300/101‑1 (MQ=255)
                                                                                         tCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccgc         <  1:865912/101‑1 (MQ=255)
                                                                                           tGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCa       <  1:1379912/101‑1 (MQ=255)
                                                                                              gcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATcc    <  2:3096542/101‑1 (MQ=255)
                                                                                               ccccGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATccg   <  2:2084289/101‑1 (MQ=255)
                                                                                                cccGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATccgc  <  1:233979/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
AAGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTG.GTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317829‑318024

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG