Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,333,494	C→G	100%	intergenic (+40/‑126)	ccmH → / → paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,333,494	0	C→G	100.0%	94.8	25	intergenic (+40/‑126)	ccmH/paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  new base (0/25):  ref base (0/0):  total (0/25)

CGGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333396‑3333591                                                                                                   |                                                                                                  gggggccggtgcTCTCGGCGGAGGAAGAGGCGCGGGTGAAAGACCTGCTGAGGGACTAGGGATTTCGCCGGGGGCTTGCGCCCCGGGCGACCCgcgggggg                                                                                                 <  1:32878/97‑1 (MQ=255)  ggtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGgggggc                                                                                                <  2:1332814/101‑1 (MQ=255)   gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  2:2429658/101‑1 (MQ=255)   gtgccggtgcGCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  2:1788937/101‑1 (MQ=255)     gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  1:2382721/101‑1 (MQ=255)     gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATGTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  2:3963127/101‑1 (MQ=255)         gtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccc                                                                                         <  1:1926742/101‑1 (MQ=255)         gtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccc                                                                                         <  1:2117600/101‑1 (MQ=255)           gcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccccc                                                                                       <  2:3144300/101‑1 (MQ=255)           gcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccccc                                                                                       <  1:3221431/101‑1 (MQ=255)              ctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGc                                                                                    <  1:2688702/101‑1 (MQ=255)                  gCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccc                                                                                <  1:313837/101‑1 (MQ=255)                   gcGAGGAAGAGGCGCGGCGGAAAGACCTGATGAAGGACTAGGGATTCCGCCCGGGGCTTGGGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccc                                                                               <  1:3951362/100‑1 (MQ=37)                   ccGAGGAAGAGGCGGGGCTGAAAGGCCTGCGGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGGCCCGCGGGGGGCTCCGCCCCCCGCAcccc                                                                               <  2:2603542/101‑1 (MQ=38)                    cGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccccc                                                                              <  1:3189050/101‑1 (MQ=255)                     gAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccccc                                                                             <  1:1336692/101‑1 (MQ=255)                         aaGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGc                                                                         <  1:3240671/101‑1 (MQ=255)                         aaGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGc                                                                         <  1:503018/101‑1 (MQ=255)                               cgcgGCGGAAAGACCTGCTGAAGGCGTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTAttt                                                                   <  1:830792/101‑1 (MQ=37)                                    cTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCa                                                              <  1:525094/101‑1 (MQ=255)                                          gACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGaaa                                                        <  2:2917219/101‑1 (MQ=255)                                                ctgAAGGACTAGGGTTTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCggg                                                  <  2:1558655/101‑1 (MQ=255)                                                         tAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTc                                         <  1:3437445/101‑1 (MQ=255)                                                              aTTTCGCCGGGGGCTGGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGAc                                    <  1:1171364/101‑1 (MQ=255)                                                              aTTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGAc                                    <  2:1340171/101‑1 (MQ=255)                                                                           cTTGCGCCGCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCt                       <  2:635721/101‑1 (MQ=255)                                                                                      ggCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCgg            <  2:447848/101‑1 (MQ=255)                                                                                       gCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGgc           <  2:1651418/101‑1 (MQ=255)                                                                                            cgggggggggCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCcg      <  2:1503268/97‑1 (MQ=255)                                                                                                ggggggCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTTGCTTTGCCTTTCGGCGCCcgcgcg  >  1:598387/1‑101 (MQ=255)                                                                                                   |                                                                                                  CGGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333396‑3333591 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG