Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,342,548	A→G	100%	V160A (GTG→GCG)	RCAP_rcc03145 ←	AsnC/Lrp family transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,342,548	0	A→G	100.0%	149.4	40	V160A (GTG→GCG)	RCAP_rcc03145	AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (1/39):  ref base (0/0):  total (1/39)

TCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGC  >  NC_014034/3342450‑3342592                                                                                                   |                                             tCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcc                                            <  2:2130804/101‑1 (MQ=255) tCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcc                                            <  2:2010862/101‑1 (MQ=255) tCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcc                                            <  1:915134/101‑1 (MQ=255) tCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcc                                            <  1:2333216/101‑1 (MQ=255)  cgcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGccc                                           <  2:1870794/101‑1 (MQ=255)  cgcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGccc                                           <  2:218458/101‑1 (MQ=255)  cgaggACGGAGGTCAGCGTCTTGGGGCTGATCTCGTACAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGccc                                           <  2:1788031/98‑1 (MQ=255)   gcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcccg                                          <  1:2876266/101‑2 (MQ=255)      gACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCa                                       <  1:815499/101‑1 (MQ=255)      gACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCa                                       <  1:754202/101‑1 (MQ=255)       gccGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAc                                      <  1:472242/100‑1 (MQ=255)       aCCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGGCCGCGGGGACCCGCGCGGGCTTGCGCACCACGCCCCGcgc                                      >  2:2356535/1‑99 (MQ=255)       aCCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAc                                      <  1:3219545/101‑1 (MQ=255)        ccGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAcc                                     <  2:30522/101‑1 (MQ=255)          gAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGGCCGCGGGCACCCGCGCGGGCTTTCGCACGACCCCCCGCCcccg                                   >  1:1028102/1‑99 (MQ=255)              ccAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGcc                               <  1:76518/101‑2 (MQ=255)               cAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGccg                              <  1:1507784/101‑3 (MQ=255)                 gCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcg                            <  2:1656459/101‑2 (MQ=255)                 gCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcg                            <  2:3181144/101‑2 (MQ=255)                  cGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcgg                           <  1:2027517/101‑3 (MQ=255)                    tctcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcg                         <  2:947424/101‑1 (MQ=255)                    tctcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcg                         <  2:3400133/101‑1 (MQ=255)                       cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                      <  2:1490759/101‑1 (MQ=255)                       cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                      <  2:2292321/101‑1 (MQ=255)                         gggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccgc                    <  1:2356711/101‑1 (MQ=255)                          ggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccgcg                   <  1:862094/101‑1 (MQ=255)                              gATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGtt               <  2:1684545/101‑1 (MQ=255)                              gATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGtt               <  1:3922265/101‑1 (MQ=255)                                    gTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcg         <  1:3601048/101‑2 (MQ=255)                                    gTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGGTGGGGcg         >  1:2664796/1‑100 (MQ=255)                                     ttCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCgaagg        <  2:2204992/101‑5 (MQ=255)                                     ttCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcgg        <  1:1010495/101‑3 (MQ=255)                                      tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc       <  1:479529/101‑4 (MQ=255)                                      tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc       <  1:1818758/101‑4 (MQ=255)                                      tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc       <  1:1141275/101‑4 (MQ=255)                                       cAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggca      <  2:413405/101‑5 (MQ=255)                                        aGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcac     <  2:1540419/101‑6 (MQ=255)                                         gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc    <  2:2254485/101‑7 (MQ=255)                                          cGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccg   <  2:1121408/101‑8 (MQ=255)                                          cGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccg   <  2:1028011/101‑8 (MQ=255)                                          cGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccg   <  1:1682146/101‑8 (MQ=255)                                           gAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccgg  <  2:2164614/101‑9 (MQ=255)                                                                                                   |                                             TCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGC  >  NC_014034/3342450‑3342592 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG