Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,788 A→G 4.6% intergenic (‑91/+72) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7880A→G 4.6% 5.9 ‑0.9 ‑0.0131intergenic (‑91/+72)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (49/76):  total (50/81)
Fisher's exact test strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.74e-01

Only 100 of 185 total aligned reads displayed.

GCAGGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGC  >  NC_014034/3439688‑3439890
                                                                                                      |                                                                                                           
gcaggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATa                                                                                                             <  1:2114376/101‑1 (MQ=255)
 caggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATaa                                                                                                            <  1:777658/101‑1 (MQ=255)
 caggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATaa                                                                                                            <  1:3895100/101‑1 (MQ=255)
 caggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATaa                                                                                                            >  2:1501797/1‑101 (MQ=255)
 caggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATaa                                                                                                            <  2:2346650/101‑1 (MQ=255)
 caggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATaa                                                                                                            <  2:301795/101‑1 (MQ=255)
  aggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAGG.GTaaa                                                                                                           >  2:2664915/1‑101 (MQ=255)
   ggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAg                                                                                                          <  1:2159162/101‑1 (MQ=255)
    gcccATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAgg                                                                                                         <  2:3565995/98‑1 (MQ=255)
      tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAgggg                                                                                                       <  1:2629441/101‑1 (MQ=255)
      tCATGGCGGCGACCATACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAgggg                                                                                                       <  2:2816322/101‑1 (MQ=255)
       cATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGGAAA.AAAAAgggg                                                                                                       >  2:3098740/1‑101 (MQ=255)
        aTGGCGGCGACCCTACCCGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgct                                                                                                     <  1:437894/101‑1 (MQ=255)
        aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAG.AAaaaaagggt                                                                                                     >  1:4106387/1‑95 (MQ=255)
         tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTG.TTTTGTGGGAG.ACAAAggggggt                                                                                                    >  1:3141661/1‑98 (MQ=255)
         tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAG.ATAAAGGGgcgt                                                                                                    >  1:1051841/1‑99 (MQ=255)
          ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgcttt                                                                                                   <  1:245989/101‑1 (MQ=255)
          ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAGA.GTTGGGGGAAG.ATAAAAGGggttt                                                                                                   >  2:372586/1‑101 (MQ=255)
           gcggggACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttg                                                                                                  <  2:1720204/101‑1 (MQ=255)
             ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGGAG.AAAAAAGGgggttgc.t                                                                                               >  1:1750672/1‑101 (MQ=255)
              gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGTCCGATGCCTCCGGCGGGTATA.TTTGGGGCAAG.ATAAAGGGgctttgc.tt                                                                                              <  2:302200/101‑1 (MQ=255)
               cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttgc.ttt                                                                                             <  2:863249/101‑1 (MQ=255)
                gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.tttt                                                                                            <  1:1283456/101‑1 (MQ=255)
                 aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTa                                                                                           <  1:1266064/101‑1 (MQ=255)
                   ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATc                                                                                         <  2:3210269/101‑1 (MQ=255)
                    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAG.ATAAAAGGGGTTTTC.tttttttt                                                                                        >  1:3002913/1‑97 (MQ=255)
                    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAACA.AAAAAGGGGTTTTGT.ttttttt                                                                                         >  2:4172186/1‑98 (MQ=255)
                    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCt                                                                                        <  2:1066214/101‑1 (MQ=255)
                    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCt                                                                                        <  1:901719/101‑1 (MQ=255)
                     tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGAAAAAAGGGGGTTTGT.tttttttt                                                                                        >  1:3568472/1‑97 (MQ=255)
                     tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGGAAG.ATAAAGGGGCTTTGC.TTTTATCtt                                                                                       >  2:252536/1‑101 (MQ=255)
                     tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCtt                                                                                       <  1:3071892/101‑1 (MQ=255)
                      acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AAAAAGGGGGTTTGC.TTTTTTTttt                                                                                      >  1:3244538/1‑101 (MQ=255)
                        acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.cc                                                                                   <  1:2498113/101‑1 (MQ=255)
                         cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.ccc                                                                                  <  2:1203806/101‑1 (MQ=255)
                         cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.ccc                                                                                  <  1:1501901/101‑1 (MQ=255)
                          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                 <  1:2823542/101‑1 (MQ=255)
                          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                 <  1:3621148/101‑1 (MQ=255)
                          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                 <  2:79563/101‑1 (MQ=255)
                           gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.ACCCa                                                                                <  2:3580988/101‑1 (MQ=255)
                            cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCaa                                                                               >  2:1370196/1‑101 (MQ=255)
                            cgcgACCAGCTTTCGAAAGACAGCAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCaa                                                                               <  2:3817270/101‑1 (MQ=255)
                             gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGTTC.TTTTGGGCGCG.ATCATGGGTCTTTTG.TTTTTTTttttttcaca                                                                               >  2:3785118/1‑94 (MQ=255)
                               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AGAAAGGGGGTTTGC.TTTTATCTTT.CTCCCAata                                                                            >  2:1809060/1‑101 (MQ=255)
                               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAata                                                                            <  1:1370296/101‑1 (MQ=255)
                                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTGGGGGGAG.AAAAAGGGGGTTTTG.TTTTTTTTTT.TCCCCAatat                                                                           >  1:1777861/1‑101 (MQ=255)
                                 ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAG.ATAAAGGGGGTTTGC.TTTTTTTTTT.TCCCCAATAT.c                                                                         >  2:2565713/1‑101 (MQ=38)
                                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAG.ATAAAGGGGGTTTGC.TTTTTTCTTT.CCCCCCATAT.CCCcggg                                                                   >  2:264656/1‑101 (MQ=38)
                                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAG.ATAAAAGGGGTTTGCTTTTTTTTTTT.CCCCAAAATT.CCCggg                                                                    >  2:2289378/1‑101 (MQ=25)
                                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCgggg                                                                   <  1:572118/101‑1 (MQ=255)
                                        tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCggggg                                                                  <  1:447211/101‑1 (MQ=255)
                                        tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGACG.TTTGTGTCAAG.CTAAAGAGGCGTTGC.TTTTATATTT.TCCCCAATAT.CTCggggg                                                                  >  2:3937614/1‑101 (MQ=255)
                                          gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTg                                                                <  1:1941979/101‑1 (MQ=255)
                                          gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTg                                                                <  2:1262362/101‑1 (MQ=255)
                                           aaaGACAGGAATTCGCCCGCCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGc                                                               <  2:2954962/101‑1 (MQ=255)
                                            aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.GTAAAAGGGGTTTGC.TTTTGTTTTT.TCCCCAACATGCCCggggggag                                                               >  2:2065130/1‑98 (MQ=255)
                                            aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AGAAAGGGGCTTTGC.TTTTATTTTT.TCCCCAATAT.CCCcgggggggg                                                              >  1:3947517/1‑97 (MQ=255)
                                            aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..g                                                            <  1:706406/101‑1 (MQ=255)
                                                cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAG.ATAAAGGGGCCTTGC.CTTTTTTTTT.CCCCAAATAT.CCCCGGGGGGC..ggggg                                                        >  2:2397676/1‑101 (MQ=37)
                                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AAAAAGGGGGTTTGC.TTTTATCTTT.CCCCAAAAAT.TCCCGGGGGGC..GGGGGc                                                       >  1:2042732/1‑101 (MQ=255)
                                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                       <  2:3054981/101‑1 (MQ=255)
                                                 aGGAATTCGGCCGGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                       <  1:1560539/101‑1 (MQ=255)
                                                      ttCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGc                                                  <  2:3141430/101‑1 (MQ=255)
                                                       tgggCCTGCGGCCGATGCCTCCGGGGGGGATA.TTTGGGGCAAG.ATAAAGGGGTTTTGC.TTTTATCTTC.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGcc                                                 <  1:788578/99‑1 (MQ=255)
                                                        cGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.ATAAAGGGGGTTTTC.TTTTTTCTTT.CCCCAAATAT.TCCGGGGGTGC..GGGGGGAGAGccc                                                >  2:1665532/1‑101 (MQ=25)
                                                          gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccccc                                              <  2:4095100/101‑1 (MQ=255)
                                                            tgactttcGATGCCTCCGGCGGGGGGA.TTTGGGGCAAG.ATGAA.ACCCCTCCC.TTTCATCTTG.CCCCCAATAT.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                             >  1:1223704/8‑100 (MQ=21)
                                                             tGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                           <  2:1921254/101‑2 (MQ=255)
                                                             tGCGGCCGATGCCTCCGGCGGGGAAA.TTTTCGGGTAG.AAAAAAGGGGTTTGT.TTTTTTTTTT.TCCCCAATAT.TCCCGGGGTGT..GGGGGCAGAGcccccccc                                           >  1:1608925/1‑98 (MQ=255)
                                                              gCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcgccg                                          <  2:2488869/101‑3 (MQ=255)
                                                               ctttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATGAA.ACCCATCCC.TTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                          <  1:3200209/97‑5 (MQ=25)
                                                                tttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATGAA.ACCCATCCC.TTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGccccccgcca                                         <  2:3462133/98‑6 (MQ=25)
                                                                  ccGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcccc                                      >  2:3131188/1‑95 (MQ=255)
                                                                   cGATGCCTCCGGCGGGGATA.TTTGGGGCAAA.ATAAAGGGGGTTTTC.TTTTATCTTT.CCCCAAATAT.CCCCGGGGGGC..GGGGGGCGAGCCCCcgccgccccg                                     >  2:3786778/1‑94 (MQ=21)
                                                                    gATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACgg                                  >  2:2414051/1‑101 (MQ=255)
                                                                         cTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGGAGAGCCCC..CGCCGCCACGGTTCAc                             >  1:3418817/1‑101 (MQ=255)
                                                                                ggggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                      >  2:3677345/1‑101 (MQ=255)
                                                                                 gggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                     >  2:387066/1‑101 (MQ=255)
                                                                                  ggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                    >  1:1445749/1‑101 (MQ=255)
                                                                                    ata.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                  >  1:3696759/1‑101 (MQ=255)
                                                                                    ata.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGCTTCACGCCGCGACCGt                  >  2:1071437/1‑101 (MQ=255)
                                                                                     ta.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                 >  1:3843013/1‑101 (MQ=255)
                                                                                     ta.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                 >  2:4035909/1‑101 (MQ=255)
                                                                                      a.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                >  2:2156981/1‑101 (MQ=255)
                                                                                         ttGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc              >  2:4097893/1‑101 (MQ=255)
                                                                                         ttGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc              >  1:2577576/1‑101 (MQ=255)
                                                                                          tGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg             >  2:909812/1‑101 (MQ=255)
                                                                                          tGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg             >  2:4062856/1‑101 (MQ=255)
                                                                                            gggCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg           >  1:507060/1‑101 (MQ=255)
                                                                                              ggggaaagaaaGTGGGTTTTC.TTTTTTCCCTCCCCCAAATGA.CCCGGGGG.GG..GGGGGGGCCGCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCgg            <  2:2042594/93‑1 (MQ=255)
                                                                                                aaG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg       >  2:3347423/1‑101 (MQ=255)
                                                                                                aaG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg       >  1:1922532/1‑101 (MQ=255)
                                                                                                aaG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg       >  1:2570410/1‑101 (MQ=255)
                                                                                                aaG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCg       >  1:4122937/1‑101 (MQ=255)
                                                                                                     tAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg   >  1:1406094/1‑101 (MQ=255)
                                                                                                     tAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg   >  2:2131848/1‑101 (MQ=255)
                                                                                                      aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc  >  2:1693072/1‑101 (MQ=255)
                                                                                                      aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc  >  2:1454805/1‑101 (MQ=255)
                                                                                                      aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc  >  1:1145281/1‑101 (MQ=255)
                                                                                                      aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACACCGCGACCGTCTCCGGGGTCGCCAgc  >  2:460712/1‑101 (MQ=255)
                                                                                                      |                                                                                                           
GCAGGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGC  >  NC_014034/3439688‑3439890

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG