Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,793 G→C 3.7% intergenic (‑96/+67) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7930G→C 3.7% 4.2 ‑2.7 ‑0.0136intergenic (‑96/+67)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (0/5):  ref base (59/71):  total (60/76)
Fisher's exact test strand distribution p-value = 6.77e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.80e-01

Only 100 of 182 total aligned reads displayed.

TCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGAC  >  NC_014034/3439694‑3439895
                                                                                                      |                                                                                                          
tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGCAG.AT.AAAaggg                                                                                                           >  2:3153372/1‑101 (MQ=255)
tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAgggg                                                                                                           <  1:2629441/101‑1 (MQ=255)
 cATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGGAAA.AA.AAAgggg                                                                                                           >  2:3098740/1‑101 (MQ=255)
  aTGGCGGCGACCCTACCCGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGgct                                                                                                         <  1:437894/101‑1 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTG.TTTTGTGGGAG.AC.AAAggggggt                                                                                                        >  1:3141661/1‑98 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAG.AT.AAAGGGgcgt                                                                                                        >  1:1051841/1‑99 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGgctt                                                                                                        <  1:3913288/101‑1 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGgctt                                                                                                        <  1:4028577/101‑1 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGgctt                                                                                                        <  2:3952341/101‑1 (MQ=255)
    ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGgcttt                                                                                                       <  1:245989/101‑1 (MQ=255)
    ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAGA.GTTGGGGGAAG.AT.AAAAGGggttt                                                                                                       >  2:372586/1‑101 (MQ=255)
      cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGgctttgc                                                                                                     <  2:236498/101‑1 (MQ=255)
      cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGgctttgc                                                                                                     <  2:3218413/101‑1 (MQ=255)
       ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGGAG.AA.AAAAGGgggttgc.t                                                                                                   >  1:1750672/1‑101 (MQ=255)
        gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGTCCGATGCCTCCGGCGGGTATA.TTTGGGGCAAG.AT.AAAGGGgctttgc.tt                                                                                                  <  2:302200/101‑1 (MQ=255)
         cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGgctttgc.ttt                                                                                                 <  2:863249/101‑1 (MQ=255)
          gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.tttt                                                                                                <  1:1283456/101‑1 (MQ=255)
           aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGCAAG.AT.AAAAGGGCTTTAC.ttttt                                                                                               >  2:2054358/1‑100 (MQ=255)
           aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTa                                                                                               <  1:1266064/101‑1 (MQ=255)
              cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAACA.AA.AAAGGGGTTTTGT.ttttttt                                                                                             >  2:4172186/1‑98 (MQ=255)
               tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGCAAG.AT.AAAAGGGGTTTTG.TTTTTTCtt                                                                                           >  2:2841015/1‑101 (MQ=37)
               tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGAAA.AAAGGGGGTTTGT.tttttttt                                                                                            >  1:3568472/1‑97 (MQ=255)
                acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AA.AAAGGGGGTTTGC.TTTTTTTttt                                                                                          >  1:3244538/1‑101 (MQ=255)
                 cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.c                                                                                        <  1:715313/101‑1 (MQ=255)
                  acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.cc                                                                                       <  1:2498113/101‑1 (MQ=255)
                   cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.ccc                                                                                      <  1:1501901/101‑1 (MQ=255)
                   cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.ccc                                                                                      <  2:1203806/101‑1 (MQ=255)
                    ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                     <  1:3621148/101‑1 (MQ=255)
                      cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCaa                                                                                   >  2:1370196/1‑101 (MQ=255)
                      cgcgACCAGCTTTCGAAAGACAGCAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCaa                                                                                   <  2:3817270/101‑1 (MQ=255)
                       gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCaaa                                                                                  <  1:2959837/101‑1 (MQ=255)
                         gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AG.AAAGGGGGTTTGC.TTTTATCTTT.CTCCCAata                                                                                >  2:1809060/1‑101 (MQ=255)
                         gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.CTTGGGGGAAG.ATAAAAGGGGCTTGGT.TTTTTTTTTT.CCCAaaaa                                                                                 >  1:2768153/1‑100 (MQ=255)
                         gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAata                                                                                <  1:1370296/101‑1 (MQ=255)
                          aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAatat                                                                               <  2:3683550/101‑1 (MQ=255)
                           ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATc                                                                              <  2:2398579/101‑1 (MQ=255)
                            cAGCTTTCGAAAGGCAGGAATTCGGCATGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATcc                                                                             <  2:1392582/101‑1 (MQ=255)
                             aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGCGAG.AG.AAAAGGGGTTTTG.TTTTTTTTTT.CCCCAAAAATtcc                                                                            >  1:1403766/1‑101 (MQ=255)
                              gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCg                                                                           <  2:1482331/101‑1 (MQ=255)
                              gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCg                                                                           <  1:1754805/101‑1 (MQ=255)
                                 ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAG.AT.AAAGGGGGTTTGC.TTTTTTCTTT.CCCCCCATATCCCcggg                                                                        >  2:264656/1‑101 (MQ=38)
                                 ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAG.AT.AAAAGGGGTTTGCTTTTTTTTTTT.CCCCAAAATTCCCggg                                                                         >  2:2289378/1‑101 (MQ=25)
                                 ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCgggg                                                                        <  1:572118/101‑1 (MQ=255)
                                  tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCggggg                                                                       <  1:447211/101‑1 (MQ=255)
                                  tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGACG.TTTGTGTCAAG.CT.AAAGAGGCGTTGC.TTTTATATTT.TCCCCAATATCTCggggg                                                                       >  2:3937614/1‑101 (MQ=255)
                                    gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTg                                                                     <  1:1941979/101‑1 (MQ=255)
                                    gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTg                                                                     <  2:1262362/101‑1 (MQ=255)
                                     aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGc                                                                    <  1:2608557/101‑1 (MQ=255)
                                     aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGc                                                                    <  2:1803824/101‑1 (MQ=255)
                                      aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAG.AT.AAAAGGGGTTTGCTTTTTTTTTTT.CCCCAAAATTCCCgggggggg                                                                    >  1:2149021/1‑98 (MQ=255)
                                          cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AT.AAAAGGGCTTTGC.TTTTATCTTT.TCCCCAATATCCCCGGGGGGC..ggggg                                                             >  2:359279/1‑101 (MQ=37)
                                          cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..ggggg                                                             <  1:492457/101‑1 (MQ=255)
                                          cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..ggggg                                                             <  1:186746/101‑1 (MQ=255)
                                           aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AA.AAAGGGGGTTTGC.TTTTATCTTT.CCCCAAAAATTCCCGGGGGGC..GGGGGc                                                            >  1:2042732/1‑101 (MQ=255)
                                           aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGc                                                            <  2:3054981/101‑1 (MQ=255)
                                           aGGAATTCGGCCGGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGc                                                            <  1:1560539/101‑1 (MQ=255)
                                             gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCag                                                          <  2:2970762/101‑1 (MQ=255)
                                               aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCagag                                                        <  2:3568219/101‑1 (MQ=255)
                                               aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCagag                                                        <  1:30428/101‑1 (MQ=255)
                                                ttCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc                                                       <  2:3141430/101‑1 (MQ=255)
                                                 tgggCCTGCGGCCGATGCCTCCGGGGGGGATA.TTTGGGGCAAG.AT.AAAGGGGTTTTGC.TTTTATCTTC.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcc                                                      <  1:788578/99‑1 (MQ=255)
                                                  cGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AT.AAAGGGGGTTTTC.TTTTTTCTTT.CCCCAAATATTCCGGGGGTGC..GGGGGGAGAGccc                                                     >  2:1665532/1‑101 (MQ=25)
                                                  cGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccc                                                     <  2:2542175/101‑1 (MQ=255)
                                                   ggCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAG.AT.AAAGGGGCGTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGGGC..GGGGGGCGAGcccc                                                    >  2:2474310/1‑101 (MQ=25)
                                                      tgactttcGATGCCTCCGGCGGGGGGA.TTTGGGGCAAG.AT.GAA.ACCCCTCCC.TTTCATCTTG.CCCCCAATATCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                  >  1:1223704/8‑100 (MQ=21)
                                                       tGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAG.AT.AAAGGGGCTTTTC.TTTTTTCTTT.CCCCAAATATTCCCGGGGTGC..CGGGGCAGAGGCCCcgcc                                                >  2:4082802/1‑100 (MQ=21)
                                                       tGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                                <  2:1921254/101‑2 (MQ=255)
                                                       tGCGGCCGATGCCTCCGGCGGGGAAA.TTTTCGGGTAG.AA.AAAAGGGGTTTGT.TTTTTTTTTT.TCCCCAATATTCCCGGGGTGT..GGGGGCAGAGcccccccc                                                >  1:1608925/1‑98 (MQ=255)
                                                        gCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcgccg                                               <  2:2488869/101‑3 (MQ=255)
                                                         ctttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.GAA.ACCCATCCC.TTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                               <  1:3200209/97‑5 (MQ=25)
                                                          tttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.GAA.ACCCATCCC.TTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcca                                              <  2:3462133/98‑6 (MQ=25)
                                                            ccGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcccc                                           >  2:3131188/1‑95 (MQ=255)
                                                             cGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.GAA.ACCCATCCC.TTTCCTCTTG.CCCCCAAAATCCCGGGGGGGCCGGGGGGCAGGGccccccgccccct                                           >  2:2805400/1‑93 (MQ=21)
                                                             cGATGCCTCCGGCGGGGATA.TTTGGGGCAAA.AT.AAAGGGGGTTTTC.TTTTATCTTT.CCCCAAATATCCCCGGGGGGC..GGGGGGCGAGCCCCcgccgccccg                                          >  2:3786778/1‑94 (MQ=21)
                                                                   cTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGGAGAGCCCC..CGCCGCCACGGTTCAc                                  >  1:3418817/1‑101 (MQ=255)
                                                                          ggggATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                           >  2:4044644/1‑101 (MQ=255)
                                                                           gggATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                          >  2:387066/1‑101 (MQ=255)
                                                                            ggATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                         >  1:1445749/1‑101 (MQ=255)
                                                                            ggATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                         >  1:80278/1‑101 (MQ=255)
                                                                              ata.tTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                       >  1:3696759/1‑101 (MQ=255)
                                                                              ata.tTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                       >  1:3783540/1‑101 (MQ=255)
                                                                              ata.tTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGCTTCACGCCGCGACCGt                       >  2:1071437/1‑101 (MQ=255)
                                                                                a.tTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                     >  2:2156981/1‑101 (MQ=255)
                                                                                   ttGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                   >  2:4097893/1‑101 (MQ=255)
                                                                                    tGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                  >  2:909812/1‑101 (MQ=255)
                                                                                       ggCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg               >  2:1709147/1‑101 (MQ=255)
                                                                                        gCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt              >  1:95133/1‑101 (MQ=255)
                                                                                         ggggaaagaaaGTGGGTTTTC.TTTTTTCCCTCCCCCAAATGACCCGGGGG.GG..GGGGGGGCCGCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCgg                 <  2:2042594/93‑1 (MQ=255)
                                                                                          aaG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg            >  1:2570410/1‑101 (MQ=255)
                                                                                          aaG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCg            >  1:4122937/1‑101 (MQ=255)
                                                                                               t.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg        >  2:2131848/1‑101 (MQ=255)
                                                                                                 aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc       >  1:1145281/1‑101 (MQ=255)
                                                                                                 aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACACCGCGACCGTCTCCGGGGTCGCCAgc       >  2:460712/1‑101 (MQ=255)
                                                                                                  aaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgcg      >  1:3084675/1‑101 (MQ=255)
                                                                                                  aaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTACACGCCGCGACCGTCTCCGGGGTCGCCAgcg      >  2:207067/1‑101 (MQ=255)
                                                                                                    ggggCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg    >  1:2251618/1‑101 (MQ=255)
                                                                                                    ggggCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg    >  2:424464/1‑101 (MQ=255)
                                                                                                     gggCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga   >  2:73671/1‑101 (MQ=255)
                                                                                                     gggCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga   >  2:817209/1‑101 (MQ=255)
                                                                                                      ggCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac  >  1:70629/1‑101 (MQ=255)
                                                                                                      |                                                                                                          
TCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.AT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGAC  >  NC_014034/3439694‑3439895

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG