Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,794 G→C 5.0% intergenic (‑97/+66) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7940G→C 5.0% 6.2 ‑2.2 ‑0.0119intergenic (‑97/+66)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (59/54):  total (60/59)
Fisher's exact test strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.78e-01

Only 100 of 183 total aligned reads displayed.

TCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACG  >  NC_014034/3439694‑3439896
                                                                                                      |                                                                                                           
tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGCAG.ATAAAaggg                                                                                                             >  2:3153372/1‑101 (MQ=255)
tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAgggg                                                                                                             <  1:2629441/101‑1 (MQ=255)
tCATGGCGGCGACCATACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAgggg                                                                                                             <  2:2816322/101‑1 (MQ=255)
 cATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGGAAA.AAAAAgggg                                                                                                             >  2:3098740/1‑101 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTG.TTTTGTGGGAG.ACAAAggggggt                                                                                                          >  1:3141661/1‑98 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAG.ATAAAGGGgcgt                                                                                                          >  1:1051841/1‑99 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctt                                                                                                          <  2:3952341/101‑1 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctt                                                                                                          <  1:4028577/101‑1 (MQ=255)
    nnnnnnnnnncTACACGNCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgcttt                                                                                                         <  2:1301177/91‑1 (MQ=255)
    ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgcttt                                                                                                         <  1:245989/101‑1 (MQ=255)
    ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAGA.GTTGGGGGAAG.ATAAAAGGggttt                                                                                                         >  2:372586/1‑101 (MQ=255)
     gcggggACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttg                                                                                                        <  2:1720204/101‑1 (MQ=255)
      cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttgc                                                                                                       <  2:3218413/101‑1 (MQ=255)
      cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttgc                                                                                                       <  2:236498/101‑1 (MQ=255)
       ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGGAG.AAAAAAGGgggttgc.t                                                                                                     >  1:1750672/1‑101 (MQ=255)
       ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttgc.t                                                                                                     <  1:704108/101‑1 (MQ=255)
        gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGTCCGATGCCTCCGGCGGGTATA.TTTGGGGCAAG.ATAAAGGGgctttgc.tt                                                                                                    <  2:302200/101‑1 (MQ=255)
         cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttgc.ttt                                                                                                   <  2:863249/101‑1 (MQ=255)
          gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.tttt                                                                                                  <  1:1283456/101‑1 (MQ=255)
           aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTa                                                                                                 <  1:1266064/101‑1 (MQ=255)
              cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAACA.AAAAAGGGGTTTTGT.ttttttt                                                                                               >  2:4172186/1‑98 (MQ=255)
               tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGAAAAAAGGGGGTTTGT.tttttttt                                                                                              >  1:3568472/1‑97 (MQ=255)
                 cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.c                                                                                          <  2:1512191/101‑1 (MQ=255)
                  acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.cc                                                                                         <  1:2498113/101‑1 (MQ=255)
                   cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.ccc                                                                                        <  1:1501901/101‑1 (MQ=255)
                    ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                       <  1:2823542/101‑1 (MQ=255)
                    ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                       <  1:3621148/101‑1 (MQ=255)
                    ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                       <  1:2429473/101‑1 (MQ=255)
                      cgcgACCAGCTTTCGAAAGACAGCAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCaa                                                                                     <  2:3817270/101‑1 (MQ=255)
                       gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCaaa                                                                                    <  1:2959837/101‑1 (MQ=255)
                         gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AGAAAGGGGGTTTGC.TTTTATCTTT.CTCCCAata                                                                                  >  2:1809060/1‑101 (MQ=255)
                         gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAata                                                                                  <  1:1370296/101‑1 (MQ=255)
                            cAGCTTTCGAAAGGCAGGAATTCGGCATGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.cc                                                                              <  2:1392582/101‑1 (MQ=255)
                             aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGCGAG.AGAAAAGGGGTTTTG.TTTTTTTTTT.CCCCAAAAAT.tcc                                                                             >  1:1403766/1‑101 (MQ=255)
                             aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.ccc                                                                             <  2:2098995/101‑1 (MQ=255)
                              gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCg                                                                            <  1:1754805/101‑1 (MQ=255)
                              gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCg                                                                            <  2:1482331/101‑1 (MQ=255)
                                 ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAG.ATAAAGGGGGTTTGC.TTTTTTCTTT.CCCCCCATAT.CCCcggg                                                                         >  2:264656/1‑101 (MQ=38)
                                 ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAG.ATAAAAGGGGTTTGCTTTTTTTTTTT.CCCCAAAATT.CCCggg                                                                          >  2:2289378/1‑101 (MQ=25)
                                 ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCgggg                                                                         <  1:572118/101‑1 (MQ=255)
                                  tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCggggg                                                                        <  1:447211/101‑1 (MQ=255)
                                  tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGACG.TTTGTGTCAAG.CTAAAGAGGCGTTGC.TTTTATATTT.TCCCCAATAT.CTCggggg                                                                        >  2:3937614/1‑101 (MQ=255)
                                    gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTg                                                                      <  1:1941979/101‑1 (MQ=255)
                                     aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGc                                                                     <  1:2608557/101‑1 (MQ=255)
                                     aaaGACAGGAATTCGCCCGCCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGc                                                                     <  2:2954962/101‑1 (MQ=255)
                                      aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.GTAAAAGGGGTTTGC.TTTTGTTTTT.TCCCCAACATGCCCggggggag                                                                     >  2:2065130/1‑98 (MQ=255)
                                      aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AGAAAGGGGCTTTGC.TTTTATTTTT.TCCCCAATAT.CCCcgggggggg                                                                    >  1:3947517/1‑97 (MQ=255)
                                      aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAG.ATAAAAGGGGTTTGCTTTTTTTTTTT.CCCCAAAATT.CCCgggggggg                                                                     >  1:2149021/1‑98 (MQ=255)
                                      aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..g                                                                  <  1:706406/101‑1 (MQ=255)
                                          cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.ATAAAAGGGCTTTGC.TTTTATCTTT.TCCCCAATAT.CCCCGGGGGGC..ggggg                                                              >  2:359279/1‑101 (MQ=37)
                                          cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAG.ATAAAGGGGCCTTGC.CTTTTTTTTT.CCCCAAATAT.CCCCGGGGGGC..ggggg                                                              >  2:2397676/1‑101 (MQ=37)
                                          cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..ggggg                                                              <  1:186746/101‑1 (MQ=255)
                                          cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..ggggg                                                              <  1:492457/101‑1 (MQ=255)
                                           aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AAAAAGGGGGTTTGC.TTTTATCTTT.CCCCAAAAAT.TCCCGGGGGGC..GGGGGc                                                             >  1:2042732/1‑101 (MQ=255)
                                           aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                             <  2:3054981/101‑1 (MQ=255)
                                           aGGAATTCGGCCGGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                             <  1:1560539/101‑1 (MQ=255)
                                               aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCagag                                                         <  1:30428/101‑1 (MQ=255)
                                               aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCagag                                                         <  2:3568219/101‑1 (MQ=255)
                                                  cGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccc                                                      <  2:2542175/101‑1 (MQ=255)
                                                    gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccccc                                                    <  2:1161113/101‑1 (MQ=255)
                                                      tgactttcGATGCCTCCGGCGGGGGGA.TTTGGGGCAAG.ATGAA.ACCCCTCCC.TTTCATCTTG.CCCCCAATAT.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                   >  1:1223704/8‑100 (MQ=21)
                                                       tGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAG.ATAAAGGGGCTTTTC.TTTTTTCTTT.CCCCAAATAT.TCCCGGGGTGC..CGGGGCAGAGGCCCcgcc                                                 >  2:4082802/1‑100 (MQ=21)
                                                       tGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                                 <  2:1921254/101‑2 (MQ=255)
                                                       tGCGGCCGATGCCTCCGGCGGGGAAA.TTTTCGGGTAG.AAAAAAGGGGTTTGT.TTTTTTTTTT.TCCCCAATAT.TCCCGGGGTGT..GGGGGCAGAGcccccccc                                                 >  1:1608925/1‑98 (MQ=255)
                                                        gCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcgccg                                                <  2:2488869/101‑3 (MQ=255)
                                                          tttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATGAA.ACCCATCCC.TTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGccccccgcca                                               <  2:3462133/98‑6 (MQ=25)
                                                            ccGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcccc                                            >  2:3131188/1‑95 (MQ=255)
                                                             cGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATGAA.ACCCATCCC.TTTCCTCTTG.CCCCCAAAAT.CCCGGGGGGGCCGGGGGGCAGGGccccccgccccct                                            >  2:2805400/1‑93 (MQ=21)
                                                             cGATGCCTCCGGCGGGGATA.TTTGGGGCAAA.ATAAAGGGGGTTTTC.TTTTATCTTT.CCCCAAATAT.CCCCGGGGGGC..GGGGGGCGAGCCCCcgccgccccg                                           >  2:3786778/1‑94 (MQ=21)
                                                              gATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACgg                                        >  2:2414051/1‑101 (MQ=255)
                                                                   cTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGGAGAGCCCC..CGCCGCCACGGTTCAc                                   >  1:3418817/1‑101 (MQ=255)
                                                                        gcggGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                              >  2:3132083/1‑101 (MQ=255)
                                                                         cggGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCCCGGTTCCCCCcgcg                             >  1:779010/1‑101 (MQ=18)
                                                                          ggggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                            >  2:4044644/1‑101 (MQ=255)
                                                                          ggggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                            >  2:3677345/1‑101 (MQ=255)
                                                                           gggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                           >  2:387066/1‑101 (MQ=255)
                                                                            ggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                          >  1:1445749/1‑101 (MQ=255)
                                                                            ggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                          >  1:80278/1‑101 (MQ=255)
                                                                              ata.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                        >  1:3696759/1‑101 (MQ=255)
                                                                              ata.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGCTTCACGCCGCGACCGt                        >  2:1071437/1‑101 (MQ=255)
                                                                               ta.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                       >  1:3843013/1‑101 (MQ=255)
                                                                                a.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                      >  2:2156981/1‑101 (MQ=255)
                                                                                   ttGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                    >  2:4097893/1‑101 (MQ=255)
                                                                                    tGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                   >  2:909812/1‑101 (MQ=255)
                                                                                     ggggCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                  >  2:1212903/1‑101 (MQ=255)
                                                                                       ggCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg                >  1:3240893/1‑101 (MQ=255)
                                                                                        ggggaaagaaaGTGGGTTTTC.TTTTTTCCCTCCCCCAAATGA.CCCGGGGG.GG..GGGGGGGCCGCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCgg                  <  2:2042594/93‑1 (MQ=255)
                                                                                        gCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt               >  1:95133/1‑101 (MQ=255)
                                                                                          aaG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg             >  1:2570410/1‑101 (MQ=255)
                                                                                          aaG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCg             >  1:4122937/1‑101 (MQ=255)
                                                                                                aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc        >  2:1693072/1‑101 (MQ=255)
                                                                                                aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc        >  1:1145281/1‑101 (MQ=255)
                                                                                                aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc        >  1:3326094/1‑101 (MQ=255)
                                                                                                aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACACCGCGACCGTCTCCGGGGTCGCCAgc        >  2:460712/1‑101 (MQ=255)
                                                                                                 aaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgcg       >  1:3084675/1‑101 (MQ=255)
                                                                                                  aGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc      >  2:2043818/1‑101 (MQ=255)
                                                                                                   ggggCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg     >  2:424464/1‑101 (MQ=255)
                                                                                                    gggCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga    >  2:73671/1‑101 (MQ=255)
                                                                                                    gggCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga    >  2:817209/1‑101 (MQ=255)
                                                                                                      gctttgc.tttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg  >  1:416338/1‑101 (MQ=255)
                                                                                                      |                                                                                                           
TCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACG  >  NC_014034/3439694‑3439896

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG