Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,796 T→A 4.7% intergenic (‑99/+64) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7960T→A 4.7% 6.2 ‑2.3 ‑0.0124intergenic (‑99/+64)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (63/54):  total (65/59)
Fisher's exact test strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 175 total aligned reads displayed.

ATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGAT  >  NC_014034/3439696‑3439898
                                                                                                      |                                                                                                            
 tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGgctt                                                                                                             <  2:3952341/101‑1 (MQ=255)
  ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGgcttt                                                                                                            <  1:245989/101‑1 (MQ=255)
  ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGgcttt                                                                                                            <  1:1990039/101‑1 (MQ=255)
     ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGgctttgc.t                                                                                                        <  1:704108/101‑1 (MQ=255)
      gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGTCCGATGCCTCCGGCGGGTATA.TTTGGGGCAAGAT.AAAGGGgctttgc.tt                                                                                                       <  2:302200/101‑1 (MQ=255)
       cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGgctttgc.ttt                                                                                                      <  2:863249/101‑1 (MQ=255)
         aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGCAAGAT.AAAAGGGCTTTAC.ttttt                                                                                                    >  2:2054358/1‑100 (MQ=255)
           ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATc                                                                                                  <  2:3210269/101‑1 (MQ=255)
            cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCt                                                                                                 <  1:901719/101‑1 (MQ=255)
             tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGCAAGAT.AAAAGGGGTTTTG.TTTTTTCtt                                                                                                >  2:2841015/1‑101 (MQ=37)
              acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGAA.AAAGGGGGTTTGC.TTTTTTTttt                                                                                               >  1:3244538/1‑101 (MQ=255)
                 cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.ccc                                                                                           <  2:1203806/101‑1 (MQ=255)
                  ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                          <  1:2429473/101‑1 (MQ=255)
                  ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                          <  2:79563/101‑1 (MQ=255)
                    cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCaa                                                                                        >  2:1370196/1‑101 (MQ=255)
                    cgcgACCAGCTTTCGAAAGACAGCAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCaa                                                                                        <  2:3817270/101‑1 (MQ=255)
                     gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGTTC.TTTTGGGCGCGAT.CATGGGTCTTTTG.TTTTTTTttttttcaca                                                                                        >  2:3785118/1‑94 (MQ=255)
                     gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCaaa                                                                                       <  1:2959837/101‑1 (MQ=255)
                      cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAat                                                                                      <  1:145754/101‑1 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGAG.AAAGGGGGTTTGC.TTTTATCTTT.CTCCCAata                                                                                     >  2:1809060/1‑101 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.CTTGGGGGAAGATAAAAGGGGCTTGGT.TTTTTTTTTT.CCCAaaaa                                                                                      >  1:2768153/1‑100 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAata                                                                                     <  2:23321/101‑1 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAata                                                                                     <  1:1370296/101‑1 (MQ=255)
                        aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAatat                                                                                    <  2:3683550/101‑1 (MQ=255)
                         ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.c                                                                                  <  2:2398579/101‑1 (MQ=255)
                          cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGCAGAAA.AAAGGGGGTTTTT.TTTTTTTTTT.CCCCaatattc                                                                                   >  1:4110608/1‑96 (MQ=255)
                           aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.ccc                                                                                <  2:2098995/101‑1 (MQ=255)
                            gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCg                                                                               <  2:1482331/101‑1 (MQ=255)
                            gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCg                                                                               <  1:1754805/101‑1 (MQ=255)
                              tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAA.ATTGGGGGAACAA.AAAGGGGCATTGA.CTTTTTGTTT.CCACCCATAT.ACCgcg                                                                             >  2:3471385/1‑99 (MQ=255)
                               ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGAT.AAAGGGGGTTTGC.TTTTTTCTTT.CCCCCCATAT.CCCcggg                                                                            >  2:264656/1‑101 (MQ=38)
                               ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGAT.AAAAGGGGTTTGCTTTTTTTTTTT.CCCCAAAATT.CCCggg                                                                             >  2:2289378/1‑101 (MQ=25)
                               ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCgggg                                                                            <  1:572118/101‑1 (MQ=255)
                                tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCggggg                                                                           <  1:447211/101‑1 (MQ=255)
                                tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGACG.TTTGTGTCAAGCT.AAAGAGGCGTTGC.TTTTATATTT.TCCCCAATAT.CTCggggg                                                                           >  2:3937614/1‑101 (MQ=255)
                                  gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTg                                                                         <  2:1262362/101‑1 (MQ=255)
                                  gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTg                                                                         <  1:3098970/101‑1 (MQ=255)
                                   aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGc                                                                        <  2:1803824/101‑1 (MQ=255)
                                   aaaGACAGGAATTCGCCCGCCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGc                                                                        <  2:2954962/101‑1 (MQ=255)
                                    aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGGT.AAAAGGGGTTTGC.TTTTGTTTTT.TCCCCAACATGCCCggggggag                                                                        >  2:2065130/1‑98 (MQ=255)
                                    aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGAG.AAAGGGGCTTTGC.TTTTATTTTT.TCCCCAATAT.CCCcgggggggg                                                                       >  1:3947517/1‑97 (MQ=255)
                                    aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGAT.AAAAGGGGTTTGCTTTTTTTTTTT.CCCCAAAATT.CCCgggggggg                                                                        >  1:2149021/1‑98 (MQ=255)
                                    aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..g                                                                     <  1:706406/101‑1 (MQ=255)
                                        cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGAT.AAAAGGGCTTTGC.TTTTATCTTT.TCCCCAATAT.CCCCGGGGGGC..ggggg                                                                 >  2:359279/1‑101 (MQ=37)
                                        cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..ggggg                                                                 <  1:492457/101‑1 (MQ=255)
                                        cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..ggggg                                                                 <  1:186746/101‑1 (MQ=255)
                                         aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                                <  2:3054981/101‑1 (MQ=255)
                                         aGGAATTCGGCCGGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                                <  1:1560539/101‑1 (MQ=255)
                                             aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCagag                                                            <  1:30428/101‑1 (MQ=255)
                                             aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCagag                                                            <  2:3568219/101‑1 (MQ=255)
                                             aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCagag                                                            <  1:356762/101‑1 (MQ=255)
                                                cGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGAT.AAAGGGGGTTTTC.TTTTTTCTTT.CCCCAAATAT.TCCGGGGGTGC..GGGGGGAGAGc.cc                                                        >  2:1665532/1‑101 (MQ=25)
                                                cGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGc.cc                                                        <  2:2542175/101‑1 (MQ=255)
                                                 ggCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAGAT.AAAGGGGCGTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGGCGAGc.ccc                                                       >  2:2474310/1‑101 (MQ=25)
                                                  gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGc.cccc                                                      <  2:420049/101‑1 (MQ=255)
                                                    tgactttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.GAA.ACCCATCCC.TTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGc.ccccc                                                     <  1:1153238/94‑2 (MQ=25)
                                                     tGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAGAT.AAAGGGGCTTTTC.TTTTTTCTTT.CCCCAAATAT.TCCCGGGGTGC..CGGGGCAGAGG.CCCcgcc                                                   >  2:4082802/1‑100 (MQ=21)
                                                     tGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCCcgcc                                                   <  2:1921254/101‑2 (MQ=255)
                                                     tGCGGCCGATGCCTCCGGCGGGGAAA.TTTTCGGGTAGAA.AAAAGGGGTTTGT.TTTTTTTTTT.TCCCCAATAT.TCCCGGGGTGT..GGGGGCAGAGc.ccccccc                                                   >  1:1608925/1‑98 (MQ=255)
                                                      gCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCCcgccg                                                  <  2:2488869/101‑3 (MQ=255)
                                                       ctttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.GAA.ACCCATCCC.TTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGc.cccccgcc                                                  <  1:3200209/97‑5 (MQ=25)
                                                         ttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.GAA.AACCATCCC.TTTCATCTTG.CCCCAAATAT.TCCGGGGGGCC..CGGGGGGGAGCGCCCCCcgccac                                               >  2:410367/3‑99 (MQ=21)
                                                          ccGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGC.CCCcgccgcccc                                              >  2:3131188/1‑95 (MQ=255)
                                                           cGATGCCTCCGGCGGGGATA.TTTGGGGCAAAAT.AAAGGGGGTTTTC.TTTTATCTTT.CCCCAAATAT.CCCCGGGGGGC..GGGGGGCGAGC.CCCcgccgccccg                                             >  2:3786778/1‑94 (MQ=21)
                                                                 cTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGGAGAGC.CCC..CGCCGCCACGGTTCAc                                     >  1:3418817/1‑101 (MQ=255)
                                                                   ccGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATTTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGC.CCC..CGCCGCCCCGGTTCAcgc                                   >  1:1551929/1‑101 (MQ=18)
                                                                      gcggGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCAcgccgc                                >  2:3132083/1‑101 (MQ=255)
                                                                       cggGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCCCGGTTCCCCCcgcg                               >  1:779010/1‑101 (MQ=18)
                                                                        ggggATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGa                              >  2:4044644/1‑101 (MQ=255)
                                                                        ggggATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGa                              >  1:1280231/1‑101 (MQ=255)
                                                                         gggATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAc                             >  2:387066/1‑101 (MQ=255)
                                                                         gggATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAc                             >  1:4128574/1‑101 (MQ=255)
                                                                          ggATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAcc                            >  1:80278/1‑101 (MQ=255)
                                                                            ata.tTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGt                          >  1:3783540/1‑101 (MQ=255)
                                                                            ata.tTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGCTTCACGCCGCGACCGt                          >  2:1071437/1‑101 (MQ=255)
                                                                             ta.tTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtc                         >  1:3843013/1‑101 (MQ=255)
                                                                              a.tTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtct                        >  2:2156981/1‑101 (MQ=255)
                                                                                 ttGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                      >  2:4097893/1‑101 (MQ=255)
                                                                                 ttGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                      >  1:2577576/1‑101 (MQ=255)
                                                                                  tGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                     >  2:909812/1‑101 (MQ=255)
                                                                                  tGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                     >  1:1233297/1‑101 (MQ=255)
                                                                                   ggggCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                    >  2:1212903/1‑101 (MQ=255)
                                                                                    gggCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                   >  1:2540940/1‑101 (MQ=255)
                                                                                     ggCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg                  >  2:1709147/1‑101 (MQ=255)
                                                                                      ggggaaagaaaGTGGGTTTTC.TTTTTTCCCTCCCCCAAATGA.CCCGGGGG.GG..GGGGGGGCCGC.CCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCgg                    <  2:2042594/93‑1 (MQ=255)
                                                                                      gCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt                 >  1:95133/1‑101 (MQ=255)
                                                                                        aaGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg               >  2:3347423/1‑101 (MQ=255)
                                                                                        aaGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg               >  1:3645745/1‑101 (MQ=255)
                                                                                        aaGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg               >  1:1922532/1‑101 (MQ=255)
                                                                                        aaGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGAAGCGACCGTCTCCgggagcg               >  1:1357121/1‑97 (MQ=21)
                                                                                            t.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg           >  1:1406094/1‑101 (MQ=255)
                                                                                              aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc          >  1:1145281/1‑101 (MQ=255)
                                                                                              aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc          >  1:3596369/1‑101 (MQ=255)
                                                                                              aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc          >  2:1454805/1‑101 (MQ=255)
                                                                                              aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACACCGCGACCGTCTCCGGGGTCGCCAgc          >  2:460712/1‑101 (MQ=255)
                                                                                               aaGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgcg         >  1:3084675/1‑101 (MQ=255)
                                                                                                aGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCGgcgc        >  1:2609069/1‑101 (MQ=255)
                                                                                                  gggCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga      >  2:73671/1‑101 (MQ=255)
                                                                                                    gctttgc.tttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg    >  1:416338/1‑101 (MQ=255)
                                                                                                     ctttgc.tttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga   >  2:2365594/1‑101 (MQ=255)
                                                                                                      |                                                                                                            
ATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGAT  >  NC_014034/3439696‑3439898

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG