Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,799 G→C 4.9% intergenic (‑102/+61) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7990G→C 4.9% 6.5 ‑2.3 ‑0.0124intergenic (‑102/+61)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (63/54):  total (65/59)
Fisher's exact test strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.04e-01

Only 100 of 170 total aligned reads displayed.

GCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATC  >  NC_014034/3439699‑3439901
                                                                                                      |                                                                                                         
gcggggACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttg                                                                                                           <  2:1720204/101‑1 (MQ=255)
 cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttgc                                                                                                          <  2:236498/101‑1 (MQ=255)
  ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttgc.t                                                                                                        <  1:704108/101‑1 (MQ=255)
   gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGTCCGATGCCTCCGGCGGGTATA.TTTGGGGCAAG.ATAAAGGGgctttgc.tt                                                                                                       <  2:302200/101‑1 (MQ=255)
    cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGgctttgc.ttt                                                                                                      <  2:863249/101‑1 (MQ=255)
     gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.tttt                                                                                                     <  1:1283456/101‑1 (MQ=255)
      aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGCAAG.ATAAAAGGGCTTTAC.ttttt                                                                                                    >  2:2054358/1‑100 (MQ=255)
        ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATc                                                                                                  <  2:3210269/101‑1 (MQ=255)
         cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAG.ATAAAAGGGGTTTTC.tttttttt                                                                                                 >  1:3002913/1‑97 (MQ=255)
         cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAACA.AAAAAGGGGTTTTGT.ttttttt                                                                                                  >  2:4172186/1‑98 (MQ=255)
         cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCt                                                                                                 <  1:901719/101‑1 (MQ=255)
         cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCt                                                                                                 <  2:1066214/101‑1 (MQ=255)
          tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGCAAG.ATAAAAGGGGTTTTG.TTTTTTCtt                                                                                                >  2:2841015/1‑101 (MQ=37)
          tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGAAAAAAGGGGGTTTGT.tttttttt                                                                                                 >  1:3568472/1‑97 (MQ=255)
           acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AAAAAGGGGGTTTGC.TTTTTTTttt                                                                                               >  1:3244538/1‑101 (MQ=255)
            cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTc                                                                                              <  2:1512191/101‑1 (MQ=255)
            cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTc                                                                                              <  1:715313/101‑1 (MQ=255)
             acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTcc                                                                                             <  1:2498113/101‑1 (MQ=255)
              cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTccc                                                                                            <  1:1501901/101‑1 (MQ=255)
               ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTcccc                                                                                           <  2:79563/101‑1 (MQ=255)
               ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTcccc                                                                                           <  1:2823542/101‑1 (MQ=255)
                 cgcgACCAGCTTTCGAAAGACAGCAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCaa                                                                                         <  2:3817270/101‑1 (MQ=255)
                  gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGTTC.TTTTGGGCGCG.ATCATGGGTCTTTTG.TTTTTTTttttttcaca                                                                                        >  2:3785118/1‑94 (MQ=255)
                    gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAata                                                                                      <  1:1370296/101‑1 (MQ=255)
                      ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATc                                                                                    <  2:2398579/101‑1 (MQ=255)
                       cAGCTTTCGAAAGGCAGGAATTCGGCATGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATcc                                                                                   <  2:1392582/101‑1 (MQ=255)
                       cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGCAGA.AAAAAGGGGGTTTTT.TTTTTTTTTTCCCCaatattc                                                                                    >  1:4110608/1‑96 (MQ=255)
                         gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCg                                                                                 <  1:1754805/101‑1 (MQ=255)
                         gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCg                                                                                 <  2:1482331/101‑1 (MQ=255)
                            ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAG.ATAAAGGGGGTTTGC.TTTTTTCTTTCCCCCCATATCCCcggg                                                                              >  2:264656/1‑101 (MQ=38)
                            ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAG.ATAAAAGGGGTTTGCTTTTTTTTTTTCCCCAAAATTCCCggg                                                                               >  2:2289378/1‑101 (MQ=25)
                               gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTg                                                                           <  2:1262362/101‑1 (MQ=255)
                                aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGc                                                                          <  2:1803824/101‑1 (MQ=255)
                                aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGc                                                                          <  1:2608557/101‑1 (MQ=255)
                                aaaGACAGGAATTCGCCCGCCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGc                                                                          <  2:2954962/101‑1 (MQ=255)
                                 aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAG.ATAAAAGGGGTTTGCTTTTTTTTTTTCCCCAAAATTCCCgggggggg                                                                          >  1:2149021/1‑98 (MQ=255)
                                     cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.ATAAAAGGGCTTTGC.TTTTATCTTTTCCCCAATATCCCCGGGGGGC..ggggg                                                                   >  2:359279/1‑101 (MQ=37)
                                     cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..ggggg                                                                   <  1:186746/101‑1 (MQ=255)
                                      aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAG.AAAAAGGGGGTTTGC.TTTTATCTTTCCCCAAAAATTCCCGGGGGGC..GGGGGc                                                                  >  1:2042732/1‑101 (MQ=255)
                                      aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGc                                                                  <  2:3054981/101‑1 (MQ=255)
                                      aGGAATTCGGCCGGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGc                                                                  <  1:1560539/101‑1 (MQ=255)
                                          aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCagag                                                              <  2:3568219/101‑1 (MQ=255)
                                          aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCagag                                                              <  1:30428/101‑1 (MQ=255)
                                            tgggCCTGCGGCCGATGCCTCCGGGGGGGATA.TTTGGGGCAAG.ATAAAGGGGTTTTGC.TTTTATCTTCCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcc                                                            <  1:788578/99‑1 (MQ=255)
                                             cGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccc                                                           <  2:2542175/101‑1 (MQ=255)
                                              ggCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAG.ATAAAGGGGCGTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGGGC..GGGGGGCGAGcccc                                                          >  2:2474310/1‑101 (MQ=25)
                                               gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccccc                                                         <  2:1161113/101‑1 (MQ=255)
                                               gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccccc                                                         <  2:420049/101‑1 (MQ=255)
                                                 tgactttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATGAA.ACCCATCCC.TTTCATCTTGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                        <  1:1153238/94‑2 (MQ=25)
                                                  tGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAG.ATAAAGGGGCTTTTC.TTTTTTCTTTCCCCAAATATTCCCGGGGTGC..CGGGGCAGAGGCCCcgcc                                                      >  2:4082802/1‑100 (MQ=21)
                                                  tGCGGCCGATGCCTCCGGCGGGGAAA.TTTTCGGGTAG.AAAAAAGGGGTTTGT.TTTTTTTTTTTCCCCAATATTCCCGGGGTGT..GGGGGCAGAGcccccccc                                                      >  1:1608925/1‑98 (MQ=255)
                                                   gCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcgccg                                                     <  2:2488869/101‑3 (MQ=255)
                                                    ctttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATGAA.ACCCATCCC.TTTCATCTTGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                                     <  1:3200209/97‑5 (MQ=25)
                                                     tttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATGAA.ACCCATCCC.TTTCATCTTGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcca                                                    <  2:3462133/98‑6 (MQ=25)
                                                              cTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGGAGAGCCCC..CGCCGCCACGGTTCAc                                        >  1:3418817/1‑101 (MQ=255)
                                                                   gcggGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                                   >  2:3132083/1‑101 (MQ=255)
                                                                    cggGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCCCGGTTCCCCCcgcg                                  >  1:779010/1‑101 (MQ=18)
                                                                     ggggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                 >  2:3677345/1‑101 (MQ=255)
                                                                     ggggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                 >  1:1280231/1‑101 (MQ=255)
                                                                      gggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                                >  1:4128574/1‑101 (MQ=255)
                                                                      gggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                                >  2:387066/1‑101 (MQ=255)
                                                                       ggATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                               >  1:1445749/1‑101 (MQ=255)
                                                                         ata.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                             >  1:3783540/1‑101 (MQ=255)
                                                                          ta.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                            >  1:3843013/1‑101 (MQ=255)
                                                                          ta.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                            >  2:4035909/1‑101 (MQ=255)
                                                                           a.tTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                           >  2:2156981/1‑101 (MQ=255)
                                                                              ttGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                         >  2:4097893/1‑101 (MQ=255)
                                                                              ttGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                         >  2:1961129/1‑101 (MQ=255)
                                                                               tGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                        >  2:4062856/1‑101 (MQ=255)
                                                                               tGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                        >  1:1233297/1‑101 (MQ=255)
                                                                               tGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                        >  2:909812/1‑101 (MQ=255)
                                                                                ggggCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                       >  2:1212903/1‑101 (MQ=255)
                                                                                 gggCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                      >  1:507060/1‑101 (MQ=255)
                                                                                  ggCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg                     >  2:3812056/1‑101 (MQ=255)
                                                                                   gCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt                    >  1:95133/1‑101 (MQ=255)
                                                                                     aaG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                  >  1:3645745/1‑101 (MQ=255)
                                                                                     aaG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                  >  1:2570410/1‑101 (MQ=255)
                                                                                     aaG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                  >  1:1922532/1‑101 (MQ=255)
                                                                                     aaG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                  >  2:3347423/1‑101 (MQ=255)
                                                                                       g.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                >  2:1841589/1‑101 (MQ=255)
                                                                                          tAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg              >  2:2131848/1‑101 (MQ=255)
                                                                                          tAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg              >  2:3117282/1‑101 (MQ=255)
                                                                                          tAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg              >  1:1406094/1‑101 (MQ=255)
                                                                                           aaaGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc             >  1:1145281/1‑101 (MQ=255)
                                                                                           aaaGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc             >  1:3596369/1‑101 (MQ=255)
                                                                                           aaaGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc             >  2:1693072/1‑101 (MQ=255)
                                                                                           aaaGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACACCGCGACCGTCTCCGGGGTCGCCAgc             >  2:460712/1‑101 (MQ=255)
                                                                                            aaGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgcg            >  1:3084675/1‑101 (MQ=255)
                                                                                            aaGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTACACGCCGCGACCGTCTCCGGGGTCGCCAgcg            >  2:207067/1‑101 (MQ=255)
                                                                                             aGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCGgcgc           >  1:2609069/1‑101 (MQ=255)
                                                                                             aGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc           >  2:2043818/1‑101 (MQ=255)
                                                                                              ggggCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg          >  1:2251618/1‑101 (MQ=255)
                                                                                               gggCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga         >  2:73671/1‑101 (MQ=255)
                                                                                                ggCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac        >  1:70629/1‑101 (MQ=255)
                                                                                                ggCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac        >  1:466618/1‑101 (MQ=255)
                                                                                                 gctttgc.tttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg       >  1:416338/1‑101 (MQ=255)
                                                                                                  ctttgc.tttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga      >  2:2365594/1‑101 (MQ=255)
                                                                                                   tttgc.tttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat     >  1:3102993/1‑101 (MQ=255)
                                                                                                     tgc.tttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat   >  2:1254624/1‑101 (MQ=255)
                                                                                                      gc.tttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc  >  1:1695183/1‑101 (MQ=255)
                                                                                                      |                                                                                                         
GCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAG.ATAAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATC  >  NC_014034/3439699‑3439901

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG