Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,804 T→C 4.2% intergenic (‑107/+56) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,8040T→C 4.2% 3.6 ‑2.5 0.0134intergenic (‑107/+56)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (75/53):  total (76/58)
Fisher's exact test strand distribution p-value = 8.46e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

GACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCA  >  NC_014034/3439704‑3439906
                                                                                                      |                                                                                                          
gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.tttt                                                                                                            <  1:1283456/101‑1 (MQ=255)
 aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGCAAGATAAAAGGGCTTTAC.ttttt                                                                                                           >  2:2054358/1‑100 (MQ=255)
    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGATAAAAGGGGTTTTC.tttttttt                                                                                                        >  1:3002913/1‑97 (MQ=255)
    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCt                                                                                                        <  1:901719/101‑1 (MQ=255)
     tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGGAAGATAAAGGGGCTTTGC.TTTTATCtt                                                                                                       >  2:252536/1‑101 (MQ=255)
      acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGAAAAAGGGGGTTTGC.TTTTTTTttt                                                                                                      >  1:3244538/1‑101 (MQ=255)
       cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.c                                                                                                    <  1:715313/101‑1 (MQ=255)
        acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.cc                                                                                                   <  1:2498113/101‑1 (MQ=255)
         cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.ccc                                                                                                  <  1:1501901/101‑1 (MQ=255)
          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                                 <  2:79563/101‑1 (MQ=255)
          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.cccc                                                                                                 <  1:2823542/101‑1 (MQ=255)
            cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCaa                                                                                               >  2:1370196/1‑101 (MQ=255)
            cgcgACCAGCTTTCGAAAGACAGCAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCaa                                                                                               <  2:3817270/101‑1 (MQ=255)
             gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGTTC.TTTTGGGCGCGATCATGGGTCTTTTG.TTTTTTTttttttcaca                                                                                               >  2:3785118/1‑94 (MQ=255)
               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAata                                                                                            <  2:23321/101‑1 (MQ=255)
               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAata                                                                                            <  1:1370296/101‑1 (MQ=255)
               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAata                                                                                            <  2:899650/101‑1 (MQ=255)
                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTGGGGGGAGAAAAAGGGGGTTTTG.TTTTTTTTTT.TCCCCAatat                                                                                           >  1:1777861/1‑101 (MQ=255)
                 ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGATAAAGGGGGTTTGC.TTTTTTTTTT.TCCCCAATATc                                                                                          >  2:2565713/1‑101 (MQ=38)
                  cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGCAGAAAAAAGGGGGTTTTT.TTTTTTTTTT.CCCCaatattc                                                                                          >  1:4110608/1‑96 (MQ=255)
                  cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATcc                                                                                         <  1:1568611/101‑1 (MQ=255)
                   aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATccc                                                                                        <  2:2098995/101‑1 (MQ=255)
                    gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCg                                                                                       <  2:1482331/101‑1 (MQ=255)
                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGATAAAGGGGGTTTGC.TTTTTTCTTT.CCCCCCATATCCCcggg                                                                                    >  2:264656/1‑101 (MQ=38)
                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGATAAAAGGGGTTTGCTTTTTTTTTTT.CCCCAAAATTCCCggg                                                                                     >  2:2289378/1‑101 (MQ=25)
                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCgggg                                                                                    <  1:572118/101‑1 (MQ=255)
                          gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTg                                                                                 <  1:1941979/101‑1 (MQ=255)
                          gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTg                                                                                 <  2:1262362/101‑1 (MQ=255)
                           aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGc                                                                                <  2:1803824/101‑1 (MQ=255)
                           aaaGACAGGAATTCGCCCGCCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGc                                                                                <  2:2954962/101‑1 (MQ=255)
                            aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGATAAAAGGGGTTTGCTTTTTTTTTTT.CCCCAAAATTCCCgggggggg                                                                                >  1:2149021/1‑98 (MQ=255)
                            aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..g                                                                             <  1:706406/101‑1 (MQ=255)
                                cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGATAAAAGGGCTTTGC.TTTTATCTTT.TCCCCAATATCCCCGGGGGGC..ggggg                                                                         >  2:359279/1‑101 (MQ=37)
                                cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAGATAAAGGGGCCTTGC.CTTTTTTTTT.CCCCAAATATCCCCGGGGGGC..ggggg                                                                         >  2:2397676/1‑101 (MQ=37)
                                cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..ggggg                                                                         <  1:186746/101‑1 (MQ=255)
                                cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..ggggg                                                                         <  1:492457/101‑1 (MQ=255)
                                 aGGAATTCGGCCGGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGc                                                                        <  1:1560539/101‑1 (MQ=255)
                                  ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGCAGATAAAGGGGCTTTGC.TTTTTTCTTT.CCCCCAAAATCCCGGGGGGGC..GGGGGcg                                                                       >  2:636460/1‑100 (MQ=255)
                                     aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCagag                                                                    <  1:356762/101‑1 (MQ=255)
                                     aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCagag                                                                    <  1:30428/101‑1 (MQ=255)
                                     aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCagag                                                                    <  2:3568219/101‑1 (MQ=255)
                                      ttCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc                                                                   <  2:3141430/101‑1 (MQ=255)
                                        cGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGATAAAGGGGGTTTTC.TTTTTTCTTT.CCCCAAATATTCCGGGGGTGC..GGGGGGAGAGc.cc                                                                >  2:1665532/1‑101 (MQ=25)
                                         ggCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAGATAAAGGGGCGTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGGGC..GGGGGGCGAGc.ccc                                                               >  2:2474310/1‑101 (MQ=25)
                                          gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.cccc                                                              <  2:1161113/101‑1 (MQ=255)
                                          gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.cccc                                                              <  2:420049/101‑1 (MQ=255)
                                            tgactttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATGAA.ACCCATCCC.TTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.ccccc                                                             <  1:1153238/94‑2 (MQ=25)
                                             tGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAGATAAAGGGGCTTTTC.TTTTTTCTTT.CCCCAAATATTCCCGGGGTGC..CGGGGCAGAGG.CCCcgcc                                                           >  2:4082802/1‑100 (MQ=21)
                                               ctttcGATGCCTCGGGGGGGAATA.TTTGGGGCAAGATGAA.CCCCACCCC.TTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.cccccgcc                                                          <  2:3045465/97‑5 (MQ=18)
                                               ctttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATGAA.ACCCATCCC.TTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.cccccgcc                                                          <  1:3200209/97‑5 (MQ=25)
                                                tttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATGAA.ACCCATCCC.TTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.cccccgcca                                                         <  2:3462133/98‑6 (MQ=25)
                                                 ttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATGAA.AACCATCCC.TTTCATCTTG.CCCCAAATATTCCGGGGGGCC..CGGGGGGGAGCGCCCCCcgccac                                                       >  2:410367/3‑99 (MQ=21)
                                                  ccGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGC.CCCcgccgcccc                                                      >  2:3131188/1‑95 (MQ=255)
                                                   cGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATGAA.ACCCATCCC.TTTCCTCTTG.CCCCCAAAATCCCGGGGGGGCCGGGGGGCAGGGc.cccccgccccct                                                      >  2:2805400/1‑93 (MQ=21)
                                                    gATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACgg                                                  >  2:2414051/1‑101 (MQ=255)
                                                         cTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGGAGAGC.CCC..CGCCGCCACGGTTCAc                                             >  1:3418817/1‑101 (MQ=255)
                                                               cggGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCCCGGTTCCCCCcgcg                                       >  1:779010/1‑101 (MQ=18)
                                                                ggggATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGa                                      >  2:3677345/1‑101 (MQ=255)
                                                                ggggATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGa                                      >  1:1280231/1‑101 (MQ=255)
                                                                ggggATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGa                                      >  2:4044644/1‑101 (MQ=255)
                                                                 gggATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAc                                     >  2:387066/1‑101 (MQ=255)
                                                                  ggATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAcc                                    >  1:1445749/1‑101 (MQ=255)
                                                                  ggATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAcc                                    >  1:80278/1‑101 (MQ=255)
                                                                    ata.tTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGt                                  >  1:3696759/1‑101 (MQ=255)
                                                                    ata.tTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGCTTCACGCCGCGACCGt                                  >  2:1071437/1‑101 (MQ=255)
                                                                     ta.tTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtc                                 >  2:4035909/1‑101 (MQ=255)
                                                                     ta.tTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtc                                 >  1:3843013/1‑101 (MQ=255)
                                                                      a.tTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtct                                >  2:2156981/1‑101 (MQ=255)
                                                                         ttGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                              >  2:1961129/1‑101 (MQ=255)
                                                                         ttGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                              >  1:2577576/1‑101 (MQ=255)
                                                                         ttGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                              >  2:4097893/1‑101 (MQ=255)
                                                                          tGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                             >  1:1233297/1‑101 (MQ=255)
                                                                           ggggCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                            >  2:1212903/1‑101 (MQ=255)
                                                                            gggCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                           >  1:2540940/1‑101 (MQ=255)
                                                                             ggggaaagaaaGTGGGTTTTC.TTTTTTCCCTCCCCCAAATGACCCGGGGG.GG..GGGGGGGCCGC.CCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCgg                            <  2:2042594/93‑1 (MQ=255)
                                                                             ggCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg                          >  2:1709147/1‑101 (MQ=255)
                                                                             ggCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg                          >  2:3812056/1‑101 (MQ=255)
                                                                              gCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt                         >  1:95133/1‑101 (MQ=255)
                                                                                aaGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                       >  2:3347423/1‑101 (MQ=255)
                                                                                aaGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                       >  1:3645745/1‑101 (MQ=255)
                                                                                aaGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCg                       >  1:4122937/1‑101 (MQ=255)
                                                                                  gATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                     >  2:1841589/1‑101 (MQ=255)
                                                                                    tAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                   >  1:1406094/1‑101 (MQ=255)
                                                                                    tAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                   >  2:3117282/1‑101 (MQ=255)
                                                                                     aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                  >  1:1145281/1‑101 (MQ=255)
                                                                                     aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                  >  1:3596369/1‑101 (MQ=255)
                                                                                     aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                  >  2:1454805/1‑101 (MQ=255)
                                                                                     aaaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACACCGCGACCGTCTCCGGGGTCGCCAgc                  >  2:460712/1‑101 (MQ=255)
                                                                                      aaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgcg                 >  1:3084675/1‑101 (MQ=255)
                                                                                      aaGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTACACGCCGCGACCGTCTCCGGGGTCGCCAgcg                 >  2:207067/1‑101 (MQ=255)
                                                                                       aGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCGgcgc                >  1:2609069/1‑101 (MQ=255)
                                                                                        ggggCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg               >  1:2251618/1‑101 (MQ=255)
                                                                                          ggCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac             >  1:466618/1‑101 (MQ=255)
                                                                                           gctttgc.tttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg            >  1:416338/1‑101 (MQ=255)
                                                                                             tttgc.tttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat          >  1:3102993/1‑101 (MQ=255)
                                                                                               tgc.tttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat        >  2:1254624/1‑101 (MQ=255)
                                                                                                gc.tttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc       >  1:2051793/1‑101 (MQ=255)
                                                                                                gc.tttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc       >  1:1695183/1‑101 (MQ=255)
                                                                                                 c.tttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATcc      >  2:2910722/1‑101 (MQ=255)
                                                                                                     ttATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc   >  1:2723386/1‑101 (MQ=255)
                                                                                                      |                                                                                                          
GACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGC.TTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCA  >  NC_014034/3439704‑3439906

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG