Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,829 T→G 5.0% intergenic (‑132/+31) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,8290T→G 5.0% 5.9 ‑3.8 0.0131intergenic (‑132/+31)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (4/5):  ref base (99/23):  total (103/28)
Fisher's exact test strand distribution p-value = 2.15e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

GAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCC  >  NC_014034/3439730‑3439929
                                                                                                    |                                                                                                      
gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTg                                                                                                       <  1:1941979/101‑1 (MQ=255)
gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTg                                                                                                       <  2:1262362/101‑1 (MQ=255)
 aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGc                                                                                                      <  1:2608557/101‑1 (MQ=255)
 aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGc                                                                                                      <  2:1803824/101‑1 (MQ=255)
 aaaGACAGGAATTCGCCCGCCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGc                                                                                                      <  2:2954962/101‑1 (MQ=255)
  aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..g                                                                                                   <  1:706406/101‑1 (MQ=255)
      cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAAGGGCTTTGCTTTTATCTTT.TCCCCAATATCCCCGGGGGGC..ggggg                                                                                               >  2:359279/1‑101 (MQ=37)
       aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGAAAAAGGGGGTTTGCTTTTATCTTT.CCCCAAAAATTCCCGGGGGGC..GGGGGc                                                                                              >  1:2042732/1‑101 (MQ=255)
       aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGc                                                                                              <  2:3054981/101‑1 (MQ=255)
        ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGCAGATAAAGGGGCTTTGCTTTTTTCTTT.CCCCCAAAATCCCGGGGGGGC..GGGGGcg                                                                                             >  2:636460/1‑100 (MQ=255)
         gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCag                                                                                            <  2:2970762/101‑1 (MQ=255)
           aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCagag                                                                                          <  1:30428/101‑1 (MQ=255)
            ttCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc                                                                                         <  2:3141430/101‑1 (MQ=255)
             tgggCCTGCGGCCGATGCCTCCGGGGGGGATATTTGGGGCAAGATAAAGGGGTTTTGCTTTTATCTTC.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcc                                                                                        <  1:788578/99‑1 (MQ=255)
              cGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAGGGGGTTTTCTTTTTTCTTT.CCCCAAATATTCCGGGGGTGC..GGGGGGAGAGccc                                                                                       >  2:1665532/1‑101 (MQ=25)
              cGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccc                                                                                       <  2:2542175/101‑1 (MQ=255)
               ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGCAAGATAAAGGGGCGTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGGGC..GGGGGGCGAGcccc                                                                                      >  2:2474310/1‑101 (MQ=25)
                gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccccc                                                                                     <  2:4095100/101‑1 (MQ=255)
                gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccccc                                                                                     <  2:420049/101‑1 (MQ=255)
                gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccccc                                                                                     <  2:1161113/101‑1 (MQ=255)
                  tgcctttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                                                    <  2:3850691/94‑2 (MQ=25)
                  tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                                                    <  1:1153238/94‑2 (MQ=25)
                   tGCGGCCGATGCCTCCGGCGGGGAAATTTTCGGGTAGAAAAAAGGGGTTTGTTTTTTTTTTT.TCCCCAATATTCCCGGGGTGT..GGGGGCAGAGcccccccc                                                                                  >  1:1608925/1‑98 (MQ=255)
                     ctttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                                                                 <  1:3200209/97‑5 (MQ=25)
                      tttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCTTG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcca                                                                                <  2:3462133/98‑6 (MQ=25)
                         cGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCCTCTTG.CCCCCAAAATCCCGGGGGGGCCGGGGGGCAGGGccccccgccccct                                                                             >  2:2805400/1‑93 (MQ=21)
                          gATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACgg                                                                         >  2:2414051/1‑101 (MQ=255)
                               cTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGGAGAGCCCC..CGCCGCCACGGTTCAc                                                                    >  1:3418817/1‑101 (MQ=255)
                                 ccGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATTTTT.CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGCCCC..CGCCGCCCCGGTTCAcgc                                                                  >  1:1551929/1‑101 (MQ=18)
                                    gcggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                                                               >  2:3132083/1‑101 (MQ=255)
                                     cggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCCCGGTTCCCCCcgcg                                                              >  1:779010/1‑101 (MQ=18)
                                      ggggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                                             >  1:1280231/1‑101 (MQ=255)
                                      ggggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                                             >  2:4044644/1‑101 (MQ=255)
                                      ggggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                                             >  2:3677345/1‑101 (MQ=255)
                                       gggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                                                            >  2:387066/1‑101 (MQ=255)
                                        ggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                                                           >  1:80278/1‑101 (MQ=255)
                                          atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                                                         >  1:3783540/1‑101 (MQ=255)
                                          atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGCTTCACGCCGCGACCGt                                                         >  2:1071437/1‑101 (MQ=255)
                                           tatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                                                        >  1:3843013/1‑101 (MQ=255)
                                            atTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                                                       >  2:2156981/1‑101 (MQ=255)
                                              ttGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                                                     >  2:4097893/1‑101 (MQ=255)
                                              ttGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                                                     >  2:1961129/1‑101 (MQ=255)
                                               tGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                                                    >  2:4062856/1‑101 (MQ=255)
                                               tGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                                                    >  1:1233297/1‑101 (MQ=255)
                                                ggggCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                                                   >  2:1212903/1‑101 (MQ=255)
                                                 gggCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                                                  >  1:507060/1‑101 (MQ=255)
                                                 gggCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                                                  >  1:2540940/1‑101 (MQ=255)
                                                  ggggaaagaaaGTGGGTTTTCTTTTTTCCCTCCCCCAAATGACCCGGGGG.GG..GGGGGGGCCGCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCgg                                                   <  2:2042594/93‑1 (MQ=255)
                                                  ggCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg                                                 >  2:1709147/1‑101 (MQ=255)
                                                  ggCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg                                                 >  1:3240893/1‑101 (MQ=255)
                                                  ggCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg                                                 >  2:3812056/1‑101 (MQ=255)
                                                   gCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt                                                >  1:95133/1‑101 (MQ=255)
                                                     aaGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                              >  2:3347423/1‑101 (MQ=255)
                                                     aaGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                              >  1:2570410/1‑101 (MQ=255)
                                                     aaGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                              >  1:1922532/1‑101 (MQ=255)
                                                     aaGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                              >  1:3645745/1‑101 (MQ=255)
                                                     aaGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCg                                              >  1:4122937/1‑101 (MQ=255)
                                                     aaGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGAAGCGACCGTCTCCgggagcg                                              >  1:1357121/1‑97 (MQ=21)
                                                       gATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                                            >  2:1841589/1‑101 (MQ=255)
                                                         tAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                          >  1:1406094/1‑101 (MQ=255)
                                                         tAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                          >  2:3117282/1‑101 (MQ=255)
                                                          aaaGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                         >  1:1145281/1‑101 (MQ=255)
                                                          aaaGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                         >  1:3326094/1‑101 (MQ=255)
                                                          aaaGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                         >  1:3596369/1‑101 (MQ=255)
                                                          aaaGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                         >  2:1454805/1‑101 (MQ=255)
                                                          aaaGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACACCGCGACCGTCTCCGGGGTCGCCAgc                                         >  2:460712/1‑101 (MQ=255)
                                                           aaGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgcg                                        >  1:3084675/1‑101 (MQ=255)
                                                           aaGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTACACGCCGCGACCGTCTCCGGGGTCGCCAgcg                                        >  2:207067/1‑101 (MQ=255)
                                                            aGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCGgcgc                                       >  1:2609069/1‑101 (MQ=255)
                                                             ggggCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg                                      >  1:2251618/1‑101 (MQ=255)
                                                              gggCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga                                     >  2:817209/1‑101 (MQ=255)
                                                               ggCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac                                    >  1:70629/1‑101 (MQ=255)
                                                               ggCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac                                    >  1:466618/1‑101 (MQ=255)
                                                                gctttgctttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg                                   >  1:416338/1‑101 (MQ=255)
                                                                 ctttgctttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga                                  >  2:2365594/1‑101 (MQ=255)
                                                                 ctttgctttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga                                  >  2:1223870/1‑101 (MQ=255)
                                                                  tttgctttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat                                 >  1:3102993/1‑101 (MQ=255)
                                                                    tgctttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat                               >  2:1254624/1‑101 (MQ=255)
                                                                     gctttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc                              >  1:1695183/1‑101 (MQ=255)
                                                                      ctttTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATcc                             >  1:2154456/1‑101 (MQ=255)
                                                                          tATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatca                         >  2:2701580/1‑101 (MQ=255)
                                                                            tCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATg                       >  2:3666087/1‑101 (MQ=255)
                                                                               tt.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATc                    >  2:1262370/1‑101 (MQ=255)
                                                                                t.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGAGATCCATCATGATcc                   >  2:2087081/1‑101 (MQ=255)
                                                                                    ccAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGt                >  1:1497596/1‑101 (MQ=255)
                                                                                    ccAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGt                >  2:2279460/1‑101 (MQ=255)
                                                                                      aaaTATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc              >  1:1023183/1‑101 (MQ=255)
                                                                                      aaaTATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc              >  2:42139/1‑101 (MQ=255)
                                                                                       aaTATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCa             >  2:2069600/1‑101 (MQ=255)
                                                                                       aaTATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCa             >  2:2562526/1‑101 (MQ=255)
                                                                                        atgcggcGGGGGCGG..GGGGGCCCCCCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc              <  1:4172490/95‑1 (MQ=255)
                                                                                        atatCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAg            >  2:3651754/1‑101 (MQ=255)
                                                                                        atatCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAg            >  2:2927568/1‑101 (MQ=255)
                                                                                         tatCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGc           >  2:760313/1‑101 (MQ=255)
                                                                                          ggcgcGGGGGGGG..GGGGGCCG.CCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGc           <  2:1880461/97‑1 (MQ=255)
                                                                                          atCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCt          >  1:428867/1‑101 (MQ=255)
                                                                                           tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTc         >  1:4010872/1‑101 (MQ=255)
                                                                                            cccGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCg        >  2:2220059/1‑101 (MQ=255)
                                                                                             ccGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGa       >  1:894001/1‑101 (MQ=255)
                                                                                                 gggTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATc   >  2:1145855/1‑101 (MQ=255)
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GAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCC  >  NC_014034/3439730‑3439929

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG