Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,382 C→A 94.5% intergenic (‑92/+38) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3820C→A94.5% 5.0 ‑1.9 ‑0.589intergenic (‑92/+38)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (33/51):  ref base (4/1):  total (37/52)
Fisher's exact test strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.86e-01

Only 100 of 118 total aligned reads displayed.

TCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461283‑3461462
                                                                                                    |                                                                                 
tCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcc                                                                                   <  2:1017659/101‑1 (MQ=255)
 cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcca                                                                                  <  2:1765116/101‑2 (MQ=255)
  tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccac                                                                                 <  2:3870014/101‑3 (MQ=255)
  tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccac                                                                                 >  2:1519294/1‑99 (MQ=255)
  tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccac                                                                                 >  1:2071083/1‑99 (MQ=255)
   ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacc                                                                                <  2:2022379/101‑4 (MQ=255)
   ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacc                                                                                >  1:3818809/1‑98 (MQ=255)
    tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccaccc                                                                               <  2:628450/101‑5 (MQ=255)
    tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccaccc                                                                               <  2:4018786/101‑5 (MQ=255)
     cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                              >  2:480499/1‑96 (MQ=255)
     cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                              >  1:2869560/1‑96 (MQ=255)
     cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                              >  1:2214587/1‑96 (MQ=255)
     cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                              >  1:1645209/1‑96 (MQ=255)
      gttgCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccgg                                                                             >  2:462140/4‑95 (MQ=255)
      aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccgg                                                                             >  1:2381071/1‑95 (MQ=255)
      aTCGCAAGGCCCACTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccgg                                                                             >  2:4218736/1‑95 (MQ=255)
        cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                           <  2:490545/101‑9 (MQ=255)
        cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                           <  2:1208182/101‑9 (MQ=255)
        cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                           <  2:2258059/101‑9 (MQ=255)
        cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                           <  2:3824788/101‑9 (MQ=255)
        cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                           >  2:3721643/1‑93 (MQ=255)
        cGCAAGGCCCCCTTTTCGCACCCGCAGATTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                           >  2:3547310/1‑93 (MQ=255)
          cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCGCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccgc                                                                         <  1:2993516/101‑11 (MQ=255)
                           aCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                  >  1:1960372/1‑101 (MQ=255)
                           aCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                  >  1:1587236/1‑101 (MQ=255)
                            cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                 >  2:1055730/1‑101 (MQ=255)
                              cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               <  1:375801/101‑1 (MQ=255)
                              cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               >  1:2956195/1‑101 (MQ=255)
                               gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCGCCCAACCCCGGTCGTTCg                                              >  2:691061/1‑101 (MQ=255)
                               gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              <  2:1421261/101‑1 (MQ=255)
                               gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              >  1:2932458/1‑101 (MQ=255)
                               gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              <  2:3434169/101‑1 (MQ=255)
                                cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                             <  2:1587127/101‑1 (MQ=255)
                                 aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                            >  2:2361684/1‑101 (MQ=255)
                                 aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                            <  1:4193295/101‑1 (MQ=255)
                                  gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                           <  2:1384849/101‑1 (MQ=255)
                                  gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                           <  1:2332343/101‑1 (MQ=255)
                                  gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                           <  1:2046514/101‑1 (MQ=255)
                                   cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                          >  1:3821162/1‑101 (MQ=255)
                                     tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                        >  2:3360871/1‑101 (MQ=255)
                                     tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                        >  2:1260584/1‑101 (MQ=255)
                                      aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       <  1:1156289/101‑1 (MQ=255)
                                      aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       <  1:539581/101‑1 (MQ=255)
                                       gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      <  2:3127948/101‑1 (MQ=255)
                                        cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     <  2:1960246/101‑1 (MQ=255)
                                        cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     >  1:711306/1‑101 (MQ=255)
                                        cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     >  2:1849717/1‑101 (MQ=255)
                                        cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     >  2:1772785/1‑101 (MQ=255)
                                        cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     <  2:3820719/101‑1 (MQ=255)
                                        cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     >  2:2773886/1‑101 (MQ=255)
                                         aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    >  1:861417/1‑101 (MQ=255)
                                         aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    <  2:300324/101‑1 (MQ=255)
                                         aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    >  1:1322331/1‑101 (MQ=255)
                                           gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                  >  1:1151701/1‑101 (MQ=255)
                                            gtGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 <  2:3123452/100‑1 (MQ=255)
                                            aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 >  2:2179578/1‑101 (MQ=255)
                                            aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 <  2:813062/101‑1 (MQ=255)
                                               cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              >  1:1937839/1‑101 (MQ=255)
                                               cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              >  1:3692048/1‑101 (MQ=255)
                                               cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              <  1:3363112/101‑1 (MQ=255)
                                                aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                             <  2:2805525/101‑1 (MQ=255)
                                                aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGCCGTTCGATCAGTTCTTGCcgacg                             >  2:554055/1‑101 (MQ=255)
                                                  gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                           <  1:682814/101‑1 (MQ=255)
                                                  gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                           <  2:2365882/101‑1 (MQ=255)
                                                   ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG......CGCAACCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          >  2:1712177/1‑101 (MQ=255)
                                                    gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                         <  1:2177893/101‑1 (MQ=255)
                                                     cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  1:1780592/101‑1 (MQ=255)
                                                     cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  1:3547563/101‑1 (MQ=255)
                                                      gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                       >  2:1416854/1‑101 (MQ=255)
                                                       cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                      >  2:2815368/1‑101 (MQ=255)
                                                       cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                      <  1:2580745/101‑1 (MQ=255)
                                                          ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                   >  2:1048760/1‑101 (MQ=255)
                                                          ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                   <  1:351951/101‑1 (MQ=255)
                                                           taGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  <  1:3728682/100‑1 (MQ=255)
                                                           cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  <  2:2901903/101‑1 (MQ=255)
                                                           cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  >  2:371598/1‑101 (MQ=255)
                                                           cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  >  2:3477766/1‑101 (MQ=255)
                                                            aGACGCAGTTTGGGGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                 <  1:1159286/101‑1 (MQ=255)
                                                            aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                 >  1:600359/1‑101 (MQ=255)
                                                              aCGCAGTTTGACGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGc               <  1:1385891/101‑1 (MQ=255)
                                                               cGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc              <  1:2361860/101‑1 (MQ=255)
                                                                gCAGTTTGGCGCAGGGCCAAAGGAAGGGGGTGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt             <  1:3975608/101‑1 (MQ=255)
                                                                gCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt             <  2:1264221/101‑1 (MQ=255)
                                                                gCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt             <  1:3721897/101‑1 (MQ=255)
                                                                 cAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt            <  2:686833/101‑1 (MQ=255)
                                                                 cAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt            <  2:3820905/101‑1 (MQ=255)
                                                                 cAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt            <  2:2501707/101‑1 (MQ=255)
                                                                  aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           >  1:1641444/1‑101 (MQ=255)
                                                                  aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           >  1:622960/1‑101 (MQ=255)
                                                                   gTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga          <  2:2989518/101‑1 (MQ=255)
                                                                   gTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga          <  1:1772904/101‑1 (MQ=255)
                                                                    tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         <  1:235793/101‑1 (MQ=255)
                                                                     ttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg        <  2:668161/101‑1 (MQ=255)
                                                                     ttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg        <  1:2007522/101‑1 (MQ=255)
                                                                       ggCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat      <  2:4164515/101‑1 (MQ=255)
                                                                       ggCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat      <  2:3475710/101‑1 (MQ=255)
                                                                        gcgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc     <  1:1245489/101‑1 (MQ=255)
                                                                         cgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg    <  2:3148410/101‑1 (MQ=255)
                                                                          gcAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc   >  2:3148705/1‑101 (MQ=255)
                                                                           cAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg  >  1:1002312/1‑101 (MQ=255)
                                                                                                    |                                                                                 
TCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461283‑3461462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG