Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,604 T→C 100% A410A (GCT→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6040T→C100.0% 708.0 187A410A (GCT→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (111/76):  ref base (0/0):  total (111/76)

Only 100 of 212 total aligned reads displayed.

CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATC  >  NC_014034/3531506‑3531700
                                                                                                  |                                                                                                
 tGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGCCGCCTAt                                                                                               >  2:2837575/1‑101 (MQ=255)
 tGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTAt                                                                                               <  1:2241600/101‑1 (MQ=255)
 tGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTAt                                                                                               >  2:2988759/1‑101 (MQ=255)
   aCCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATcc                                                                                             >  1:2744331/1‑101 (MQ=255)
     cTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                           >  1:1642515/1‑101 (MQ=255)
       acaacaACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAAc                                                                                         >  2:2555772/1‑101 (MQ=255)
        caacaaCTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACg                                                                                        >  1:1535825/1‑101 (MQ=255)
        caacaaCTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACg                                                                                        >  2:3301884/1‑101 (MQ=255)
         aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                       >  2:339583/1‑101 (MQ=255)
         aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                       >  2:1217051/1‑101 (MQ=255)
          actacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACggg                                                                                      >  1:2420417/4‑101 (MQ=255)
           caacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGc                                                                                     >  1:3979721/1‑101 (MQ=255)
            aacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGcc                                                                                    <  2:3867965/101‑1 (MQ=255)
              cTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCg                                                                                  >  1:1998022/1‑101 (MQ=255)
                  cccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCt                                                                              >  1:251449/1‑101 (MQ=255)
                   ccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtg                                                                             <  1:491634/101‑1 (MQ=255)
                    cAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtga                                                                            <  2:3568136/101‑1 (MQ=255)
                     aGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgat                                                                           >  2:2474549/1‑101 (MQ=255)
                     aGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgat                                                                           >  1:3796536/1‑101 (MQ=255)
                     aGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgat                                                                           >  1:620304/1‑101 (MQ=255)
                     aGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGACCGAGGGCACGATCACCGAAGACGCCTATCCGCACGAGCCCGAGAtgat                                                                           >  1:723045/1‑101 (MQ=255)
                      gCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatg                                                                          <  2:623968/101‑1 (MQ=255)
                      gCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatg                                                                          <  2:135279/101‑1 (MQ=255)
                      gCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatg                                                                          >  1:1569225/1‑101 (MQ=255)
                        ctCAACTGGACGCTGAACCTGCGGAAGCAGGTGCGCGCGGAGTGGCTGGCCGAGGGCACGATCATCGAAGACGCCTATCCGATCGGGCCCGAGCTGATTaa                                                                        >  2:713510/2‑101 (MQ=255)
                         tCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAg                                                                       <  2:451242/101‑1 (MQ=255)
                          cAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGc                                                                      >  2:1789811/1‑101 (MQ=255)
                            aCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGccc                                                                    <  1:3962213/101‑1 (MQ=255)
                             cTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCg                                                                   >  2:2733914/1‑101 (MQ=255)
                              tGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGa                                                                  <  1:3356486/101‑1 (MQ=255)
                                 aCGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCGGAACGGGCCCGAGCTGATGAAGCCCGACTa                                                               >  1:268620/1‑101 (MQ=255)
                                  cGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTAt                                                              <  1:1205148/101‑1 (MQ=255)
                                    cTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATga                                                            <  2:3161365/101‑1 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgag                                                           >  2:148675/1‑100 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                           >  1:2795580/1‑101 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                          >  1:2989940/1‑101 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                          <  1:795955/101‑1 (MQ=255)
                                       aaCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacga                                                         <  2:3979461/101‑1 (MQ=255)
                                        aCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacgac                                                        >  2:1166898/1‑101 (MQ=255)
                                        aCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacgac                                                        <  1:357313/101‑1 (MQ=255)
                                          cTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGacac                                                      <  1:2826116/101‑1 (MQ=255)
                                           tGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACg                                                     >  1:3525582/1‑101 (MQ=255)
                                           tGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACg                                                     <  2:2635311/101‑1 (MQ=255)
                                            gcgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGc                                                    <  1:3720927/101‑1 (MQ=255)
                                            gcgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGc                                                    >  2:1358711/1‑101 (MQ=255)
                                             cgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcc                                                   <  1:2562747/101‑1 (MQ=255)
                                              gcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGccc                                                  <  1:1099588/101‑1 (MQ=255)
                                               cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccc                                                 >  1:218623/1‑100 (MQ=255)
                                                 aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                               <  1:1402629/101‑1 (MQ=255)
                                                  gCAGGTGCGCGCGCAGTGTCTGGCCGAGGGCACGATCACCGAAGACGCCTAGCCGAAGGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                              <  2:559584/101‑1 (MQ=255)
                                                  gCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                              <  1:3860400/101‑1 (MQ=255)
                                                   cAGGTGCGGGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                             <  1:2895863/101‑1 (MQ=255)
                                                   cAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                             <  1:2474733/101‑1 (MQ=255)
                                                    aGGAGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGAAGACACGCCCCCCGGc                                            >  2:2680323/1‑101 (MQ=255)
                                                     ggTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGcc                                           >  2:2413540/1‑101 (MQ=255)
                                                      gTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcg                                          >  1:113543/1‑101 (MQ=255)
                                                       tGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcgc                                         >  2:2323346/1‑101 (MQ=255)
                                                        gcgcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcgcg                                        >  2:2010766/1‑101 (MQ=255)
                                                        gcgcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcgcg                                        >  2:3078185/1‑101 (MQ=255)
                                                           cgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAg                                     <  2:1630862/101‑1 (MQ=255)
                                                             cgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGcc                                   <  2:1997896/101‑1 (MQ=255)
                                                              gcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCg                                  <  1:1358811/101‑1 (MQ=255)
                                                              gcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCg                                  >  2:3555450/1‑101 (MQ=255)
                                                               cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                 >  1:1477169/1‑101 (MQ=255)
                                                               cAGGGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                 <  1:4076402/101‑1 (MQ=255)
                                                                aGTGGTTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                <  2:827458/101‑1 (MQ=255)
                                                                aGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                >  1:4012546/1‑101 (MQ=255)
                                                                aGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                >  2:3698322/1‑101 (MQ=255)
                                                                   ggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACTCACCTCGGCCCCGAAGCCGAtccc                             >  1:2092879/1‑98 (MQ=255)
                                                                   ggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcc                             >  1:531495/1‑100 (MQ=255)
                                                                    gctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcct                            <  2:2858792/101‑3 (MQ=255)
                                                                     ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                           >  1:2138326/1‑101 (MQ=255)
                                                                     ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGAATATGACGACACGCCCCTCGGCCGCGACGCCGCTGCCTg                           >  1:3421196/1‑101 (MQ=255)
                                                                       ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCTGATGCCTGGc                         >  2:4124176/1‑101 (MQ=255)
                                                                        gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCt                        <  1:850449/101‑1 (MQ=255)
                                                                          cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                      >  1:3107811/1‑101 (MQ=255)
                                                                           gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                     <  2:812633/101‑1 (MQ=255)
                                                                           gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                     >  2:2089828/1‑101 (MQ=255)
                                                                            aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                    >  2:3765520/1‑101 (MQ=255)
                                                                             gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                   >  2:1645834/1‑101 (MQ=255)
                                                                             gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                   >  1:2436853/1‑101 (MQ=255)
                                                                             gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                   >  1:3021473/1‑101 (MQ=255)
                                                                               acACGATCACCGAAGGCTCCACACCGAACGGGCCCGAGCTGATTAAGCCCGACTATGACGACACGCCCCTCCGCCGCAAAGCCGATGCCTGGCTCAAGGcc                 <  2:2904980/100‑1 (MQ=255)
                                                                                cACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACACCCCTCAGCCGCGAAGCCGATGCCTGGCTCAAGGCCt                >  2:294232/1‑101 (MQ=255)
                                                                                  cGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTc              >  2:3474719/1‑101 (MQ=255)
                                                                                   gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc             >  2:1966389/1‑101 (MQ=255)
                                                                                   gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc             >  2:2579659/1‑101 (MQ=255)
                                                                                    aTCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa            <  1:1145608/101‑1 (MQ=255)
                                                                                     tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg           >  1:1114441/1‑101 (MQ=255)
                                                                                     tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg           >  1:1321243/1‑101 (MQ=255)
                                                                                      cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa          >  1:749062/1‑101 (MQ=255)
                                                                                        ccGAAGCCGCCTATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc        <  2:3054838/101‑1 (MQ=255)
                                                                                          gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga      >  2:3321829/1‑101 (MQ=255)
                                                                                           aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGGAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag     >  1:628420/1‑101 (MQ=255)
                                                                                           aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag     >  2:3638140/1‑101 (MQ=255)
                                                                                           aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag     <  1:3698576/101‑1 (MQ=255)
                                                                                            aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga    >  2:749676/1‑101 (MQ=255)
                                                                                              aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc  <  2:2166902/101‑1 (MQ=255)
                                                                                              aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc  <  2:1586596/101‑1 (MQ=255)
                                                                                              aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc  >  2:868961/1‑101 (MQ=255)
                                                                                                  |                                                                                                
CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATC  >  NC_014034/3531506‑3531700

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG