Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,673 G→C 100% A433A (GCG→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6730G→C100.0% 841.1 220A433A (GCG→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (125/94):  ref base (0/0):  total (125/95)

Only 100 of 227 total aligned reads displayed.

CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770
                                                                                                  |                                                                                                 
ttggcCGAGGGCACGTTCGCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                                                 <  1:2122573/100‑1 (MQ=255)
ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGAATATGACGACACGCCCCTCGGCCGCGACGCCGCTGCCTg                                                                                                 >  1:3421196/1‑101 (MQ=255)
 tggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                                                                                                >  2:3263185/1‑101 (MQ=255)
 tggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                                                                                                >  1:2472533/1‑101 (MQ=255)
   gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCt                                                                                              <  1:850449/101‑1 (MQ=255)
    ccGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTc                                                                                             <  2:2937894/101‑1 (MQ=255)
    ccGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTc                                                                                             >  2:3763025/1‑101 (MQ=255)
      gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                                           <  2:812633/101‑1 (MQ=255)
      gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                                           >  2:2089828/1‑101 (MQ=255)
          acACGATCACCGAAGGCTCCACACCGAACGGGCCCGAGCTGATTAAGCCCGACTATGACGACACGCCCCTCCGCCGCAAAGCCGATGCCTGGCTCAAGGcc                                                                                       <  2:2904980/100‑1 (MQ=255)
            ccGACCACCGAAGACGCCTATCCGAACGGCCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                                     <  2:2474945/100‑1 (MQ=255)
             cGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTc                                                                                    >  2:3474719/1‑101 (MQ=255)
             cGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTc                                                                                    >  2:3554761/1‑101 (MQ=255)
              gaccccggAAGAGGCCTTTCGGAACGGGCCGGAGCTGAGAAAGCCCGACTATGACGACACGCCCCCCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   <  1:3818818/94‑1 (MQ=255)
              gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   <  1:2970308/101‑1 (MQ=255)
              gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   >  2:750630/1‑101 (MQ=255)
                tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 >  2:410260/1‑101 (MQ=255)
                tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 >  1:1114441/1‑101 (MQ=255)
                tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 >  1:1321243/1‑101 (MQ=255)
                 cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa                                                                                >  1:749062/1‑101 (MQ=255)
                  aCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc                                                                               >  1:2891111/1‑101 (MQ=255)
                   ccGAAGCCGCCTATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                                              <  2:3054838/101‑1 (MQ=255)
                   ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                                              <  1:2555963/101‑1 (MQ=255)
                   ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                                              <  1:199351/101‑1 (MQ=255)
                     gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga                                                                            >  2:3321829/1‑101 (MQ=255)
                      aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag                                                                           <  1:3698576/101‑1 (MQ=255)
                      aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag                                                                           >  2:3638140/1‑101 (MQ=255)
                       agccGCCTATCCGAGCTGGCCCGCGCTGATGAAGCCCGATGATGACGACACGCCCCTCGCCCTCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGATCgaga                                                                          <  2:722969/98‑1 (MQ=255)
                        gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCCCAAGGCCTTCCAGACCGAGAt                                                                         >  2:1269372/1‑101 (MQ=255)
                         aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                                        <  1:2165892/101‑1 (MQ=255)
                           gcctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgc                                                                      >  2:2144200/4‑101 (MQ=255)
                             ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgcgc                                                                    >  2:1612054/2‑100 (MQ=255)
                             ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGa                                                                    >  1:3108612/2‑101 (MQ=255)
                              tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                                                   >  2:2527211/1‑101 (MQ=255)
                              tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                                                   >  2:2187269/1‑101 (MQ=255)
                               aTCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcg                                                                  >  2:2059356/1‑101 (MQ=255)
                                 ccGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcgcg                                                                >  1:1273662/1‑101 (MQ=255)
                                  cGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgg                                                               >  1:3948459/1‑101 (MQ=255)
                                   gAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcggg                                                              >  2:1032261/1‑101 (MQ=255)
                                   gAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcggg                                                              >  2:3167566/1‑101 (MQ=255)
                                   gAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcggg                                                              >  1:2667249/1‑101 (MQ=255)
                                    accGGGCCCGAGCTGAGGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggc                                                             <  2:633027/99‑1 (MQ=255)
                                    aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGCCgcgggc                                                             >  1:1371319/1‑101 (MQ=255)
                                    aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggc                                                             <  1:1724676/101‑1 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          >  2:3596750/1‑101 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          >  2:3067469/1‑101 (MQ=255)
                                          ccggAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgt                                                       <  2:4012286/98‑1 (MQ=255)
                                            cGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGCCCGAACTCGCGACGCGGGCGGGGgggt                                                     >  2:2659438/1‑101 (MQ=255)
                                             gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGtt                                                    >  2:591352/1‑101 (MQ=255)
                                               gCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTcc                                                  <  1:1789930/101‑1 (MQ=255)
                                                gtgaggaAGCCCGACTGTGGCCACTCGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                                                 <  2:3915918/100‑1 (MQ=255)
                                                  gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                                               >  1:259668/1‑101 (MQ=255)
                                                  gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                                               >  1:2055271/1‑101 (MQ=255)
                                                   atgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACaa                                              >  1:2795031/1‑101 (MQ=255)
                                                    tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAc                                             >  2:4214158/1‑101 (MQ=255)
                                                    tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAc                                             <  1:698714/101‑1 (MQ=255)
                                                      aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                                           <  1:2734120/101‑1 (MQ=255)
                                                      aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                                           >  2:1484478/1‑101 (MQ=255)
                                                       aGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTg                                          >  1:1030357/1‑101 (MQ=255)
                                                       aGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTg                                          <  1:3763282/101‑1 (MQ=255)
                                                       aGCCCCACGATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTg                                          <  2:3420951/101‑1 (MQ=255)
                                                        gCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGa                                         >  1:3140927/1‑101 (MQ=255)
                                                          ccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATc                                       <  2:1002736/101‑1 (MQ=255)
                                                           cGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCa                                      <  1:3078415/101‑1 (MQ=255)
                                                           cGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCa                                      <  2:1126615/101‑1 (MQ=255)
                                                           cGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCa                                      >  2:3004286/1‑101 (MQ=255)
                                                            gACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAc                                     >  1:114658/1‑101 (MQ=255)
                                                            gACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAc                                     <  2:2327189/101‑1 (MQ=255)
                                                                aTGACGACACGCCTCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTg                                 <  1:3638393/101‑1 (MQ=255)
                                                                aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGCCGCGGGCGGGGGCGTTCCACAACCTGATCACCCTg                                 >  2:3771923/1‑101 (MQ=255)
                                                                aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTg                                 <  2:198620/101‑1 (MQ=255)
                                                                aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTg                                 >  1:2994818/1‑101 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa                             >  1:3328176/1‑101 (MQ=255)
                                                                      acacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCAcc                           <  1:2413716/101‑1 (MQ=255)
                                                                        acGCCCCTCGGCGGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         <  1:4155558/101‑1 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCTTGGCTCAAGGCCTTCCAGCCCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         <  1:3555703/101‑1 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         >  2:899302/1‑101 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         >  1:3457019/1‑101 (MQ=255)
                                                                           cccCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCGTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCa                      <  2:1171877/101‑1 (MQ=255)
                                                                              ctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCt                   <  2:1114347/100‑1 (MQ=255)
                                                                              ctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCt                   >  1:1677357/2‑101 (MQ=255)
                                                                               ccGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTg                  <  2:1734999/100‑1 (MQ=255)
                                                                                cGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGa                 <  1:3004511/101‑1 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                >  1:429095/1‑101 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                >  1:593449/1‑101 (MQ=255)
                                                                                  gCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAcc               <  2:2602535/101‑1 (MQ=255)
                                                                                   ccGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACcg              >  2:1831587/1‑101 (MQ=255)
                                                                                    gacggggtcGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACATCCTGATCCCCCTGCCGACCTTCCATCTGACcgc             >  2:1392292/9‑101 (MQ=255)
                                                                                     gcgAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACcgcg            <  1:23808/101‑1 (MQ=255)
                                                                                       gAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaa          <  1:1484580/101‑1 (MQ=255)
                                                                                       gAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaa          >  2:744423/1‑101 (MQ=255)
                                                                                        aaGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaaa         <  1:2988981/101‑1 (MQ=255)
                                                                                        aaGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaaa         <  2:2133841/101‑1 (MQ=255)
                                                                                        aaGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCCGATCACCCTGCCCACCTTCCATCTGACCGCGaaa         >  1:573673/1‑101 (MQ=255)
                                                                                         aGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAAt        >  1:1608432/1‑101 (MQ=255)
                                                                                          gCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATc       <  1:3167805/101‑1 (MQ=255)
                                                                                           ccGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCg      >  1:119543/1‑101 (MQ=255)
                                                                                             gATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgat    >  2:4188592/1‑101 (MQ=255)
                                                                                              aTGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatg   >  2:15457/1‑101 (MQ=255)
                                                                                               tGCCTGGCTCAATGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatgg  >  1:2298255/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG