Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,541,575 G→C 100% S53S (TCG→TCC prpB → methylisocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,541,5750G→C100.0% 751.2 198S53S (TCG→TCCprpBmethylisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (94/104):  ref base (0/0):  total (94/104)

Only 100 of 207 total aligned reads displayed.

CCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTC  >  NC_014034/3541478‑3541670
                                                                                                 |                                                                                               
cccTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGgc                                                                                              <  1:3975190/101‑1 (MQ=255)
 ccTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGgcg                                                                                             <  1:3931337/101‑1 (MQ=255)
   tGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGGcgcg                                                                                           >  2:492130/1‑101 (MQ=255)
    ggcccggcccGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCgcgg                                                                                          <  2:2987543/101‑1 (MQ=255)
        gggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGAt                                                                                      >  2:3496924/2‑101 (MQ=255)
        cggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGAt                                                                                      >  1:2823329/1‑101 (MQ=255)
        cggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGAt                                                                                      >  1:2565781/1‑101 (MQ=255)
         ggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATg                                                                                     >  2:3195061/1‑101 (MQ=255)
          gcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGt                                                                                    >  1:3010426/1‑101 (MQ=255)
             cggcATCCTGCGGCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCgg                                                                                 >  1:2289787/1‑101 (MQ=255)
             cggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCgg                                                                                 >  1:2654633/1‑101 (MQ=255)
                cATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCt                                                                              <  2:790374/101‑1 (MQ=255)
                 aTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTc                                                                             <  2:2249770/101‑1 (MQ=255)
                 aTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTc                                                                             <  2:1314298/101‑1 (MQ=255)
                  tCCTGCGCCTACCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCg                                                                            >  1:838108/1‑101 (MQ=255)
                    cTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGAt                                                                          <  1:1087113/101‑1 (MQ=255)
                        gcCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGc                                                                      <  2:1954384/101‑1 (MQ=255)
                         ccTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGcc                                                                     >  2:3987803/1‑101 (MQ=255)
                          cTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCt                                                                    >  1:4072275/1‑101 (MQ=255)
                           tGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTg                                                                   >  2:2199129/1‑101 (MQ=255)
                               gggCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCgg                                                               >  1:469772/1‑101 (MQ=255)
                                 gcgcgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGAc                                                             <  2:1137610/101‑1 (MQ=255)
                                  cgcgcATAACGGCATGGCCGCGCTTCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGAcc                                                            <  2:2099339/101‑1 (MQ=255)
                                  cgcgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGAcc                                                            <  1:2788014/101‑1 (MQ=255)
                                    cgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTc                                                          <  2:1212818/101‑1 (MQ=255)
                                     gcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCg                                                         >  2:1673118/1‑101 (MQ=255)
                                      cATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCgg                                                        >  1:3568063/1‑101 (MQ=255)
                                         aaCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCAt                                                     >  2:2428452/1‑101 (MQ=255)
                                         aaCGGCATGGCCGCGCTGCAGGCGAAAGCCCCGGGGGTTCCGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCac                                                     >  1:801436/1‑100 (MQ=255)
                                           cGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATcc                                                   >  2:2114891/1‑101 (MQ=255)
                                            ggCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCt                                                  <  1:2046334/101‑1 (MQ=255)
                                              cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGa                                                >  1:1209842/1‑101 (MQ=255)
                                              cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGa                                                >  1:1604841/1‑101 (MQ=255)
                                              cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGa                                                >  1:3292075/1‑101 (MQ=255)
                                               aTGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGAc                                               >  2:2941958/1‑101 (MQ=255)
                                               aTGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGAc                                               <  1:2132608/101‑1 (MQ=255)
                                                 ggCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACgg                                             >  2:3818447/1‑101 (MQ=255)
                                                  gCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGt                                            <  1:2692036/101‑1 (MQ=255)
                                                   ccGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTg                                           <  1:4147757/101‑1 (MQ=255)
                                                   ccGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGCCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTg                                           <  1:1053286/101‑1 (MQ=255)
                                                    cgcgCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTgg                                          >  1:38726/1‑101 (MQ=255)
                                                     gcgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGa                                         <  1:18143/101‑1 (MQ=255)
                                                     gcgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGa                                         <  2:2716338/101‑1 (MQ=255)
                                                      cgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGAc                                        >  2:3313420/1‑101 (MQ=255)
                                                      cgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGAc                                        >  1:1627678/1‑101 (MQ=255)
                                                       gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                       >  2:1282715/1‑101 (MQ=255)
                                                        cTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACgg                                      >  2:3756652/1‑100 (MQ=255)
                                                        cTGCAGGAGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGa                                      >  2:2069636/1‑101 (MQ=255)
                                                         tGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAg                                     >  2:58740/1‑101 (MQ=255)
                                                         tGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAg                                     >  2:259233/1‑101 (MQ=255)
                                                         tGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCCGTCCGGCGCGGCGATGACGTCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTAGACGAg                                     <  2:688360/101‑1 (MQ=255)
                                                         tGCAGGCGAAAGCCGCGGCGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAg                                     <  2:1728749/101‑1 (MQ=255)
                                                          gCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAgg                                    >  2:1817119/1‑101 (MQ=255)
                                                           cAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAggg                                   >  2:3841107/1‑100 (MQ=255)
                                                           cAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGt                                   >  1:198205/1‑101 (MQ=255)
                                                            aGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                  <  1:3497176/101‑1 (MQ=255)
                                                            aGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                  <  2:440269/101‑1 (MQ=255)
                                                               cGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGcc                               <  2:1881021/101‑1 (MQ=255)
                                                                 aaaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtt                             <  1:3693758/101‑1 (MQ=255)
                                                                   aGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtttt                           >  2:106350/1‑101 (MQ=255)
                                                                   aGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtttt                           >  1:3147868/1‑101 (MQ=255)
                                                                   aGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtttt                           <  2:497332/101‑1 (MQ=255)
                                                                     ccGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTc                         <  2:1611076/101‑1 (MQ=255)
                                                                     ccGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTc                         <  1:1309118/101‑1 (MQ=255)
                                                                       gcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCAt                       <  2:3619048/101‑1 (MQ=255)
                                                                       gcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCAt                       <  1:1914843/101‑1 (MQ=255)
                                                                       gcgGGGTTTCAGGCGCTTTATCTGGCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCAt                       <  2:959650/101‑1 (MQ=255)
                                                                        cgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATc                      >  2:4133003/1‑101 (MQ=255)
                                                                        cgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATc                      >  1:2916132/1‑101 (MQ=255)
                                                                        cgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATc                      <  1:3036607/101‑1 (MQ=255)
                                                                         ggggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATcc                     <  1:1009244/101‑1 (MQ=255)
                                                                         ggggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATcc                     <  2:2294273/101‑1 (MQ=255)
                                                                           ggcttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGc                   <  1:2314989/98‑1 (MQ=255)
                                                                           ggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGc                   >  2:3587691/1‑101 (MQ=255)
                                                                            cttccgatctcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGcc                  <  2:3062275/91‑1 (MQ=255)
                                                                             tttCAGGCGCTTTATCTGTCCGGCTCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCa                 <  2:2395305/101‑1 (MQ=255)
                                                                             tttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCa                 <  1:226337/101‑1 (MQ=255)
                                                                             gttCAGGCGCTTGCTCTGTCCGGCGCGTCGAGGTCAGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCa                 <  2:3153631/100‑1 (MQ=255)
                                                                               tCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGa               <  2:202619/101‑1 (MQ=255)
                                                                                cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGAt              <  2:3010201/101‑1 (MQ=255)
                                                                                cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGNGGACGAGGTCGCCTTTTTCATCCGCCAGAt              >  1:422546/1‑101 (MQ=255)
                                                                                cAGCCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGAt              <  2:2823110/101‑1 (MQ=255)
                                                                                 aGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATc             >  2:409631/1‑101 (MQ=255)
                                                                                 aGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATc             >  1:1736152/1‑101 (MQ=255)
                                                                                  ggCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCa            <  2:3108591/101‑1 (MQ=255)
                                                                                  ggCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCa            <  2:805332/101‑1 (MQ=255)
                                                                                  ggCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCa            <  1:1445675/101‑1 (MQ=255)
                                                                                   gcgcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAc           <  1:55384/101‑1 (MQ=255)
                                                                                     gcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcgc         <  2:2104557/101‑1 (MQ=255)
                                                                                      cTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcgcg        <  2:2899542/101‑1 (MQ=255)
                                                                                      cTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcgcg        <  2:2471370/101‑1 (MQ=255)
                                                                                        ttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCggg      >  1:1313265/1‑101 (MQ=255)
                                                                                         tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc     >  2:2521881/1‑101 (MQ=255)
                                                                                         tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc     <  1:3456721/101‑1 (MQ=255)
                                                                                         tATCTGTCCGGCGCGGCGAGGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc     <  2:2957270/101‑1 (MQ=255)
                                                                                           tCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCt   >  1:2920811/1‑101 (MQ=255)
                                                                                           tCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCt   <  1:3195300/101‑1 (MQ=255)
                                                                                           tCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACATCGGCATCCTGACGGTGGAGGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCt   >  1:3619190/1‑101 (MQ=255)
                                                                                            cTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTc  <  1:2020266/101‑1 (MQ=255)
                                                                                            cTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTc  <  1:2364124/101‑1 (MQ=255)
                                                                                                 |                                                                                               
CCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTC  >  NC_014034/3541478‑3541670

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG