Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,542,001 T→C 100% T195T (ACT→ACC prpB → methylisocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,542,0010T→C100.0% 536.8 141T195T (ACT→ACCprpBmethylisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (58/83):  ref base (0/0):  total (58/83)

Only 100 of 175 total aligned reads displayed.

ACCGATGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACTTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGACCGCGG  >  NC_014034/3541903‑3542098
                                                                                                  |                                                                                                 
 ccGATGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCg                                                                                                <  1:4168981/101‑1 (MQ=255)
 ccGATGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCg                                                                                                <  1:3924902/101‑1 (MQ=255)
  cGATGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCgg                                                                                               <  1:2794273/101‑1 (MQ=255)
    aTGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGGGCCGATGCGATCTTCCCCCGGGCGCTGACCCCGGTc                                                                                             >  1:784387/1‑101 (MQ=255)
    aTGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTc                                                                                             <  1:1040199/101‑1 (MQ=255)
     tGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGGGCCGGTGGGGTCTTTCCCCAGGCGCTGACCTccggcg                                                                                            >  2:3544820/1‑96 (MQ=255)
     tGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCg                                                                                            <  1:3877457/101‑1 (MQ=255)
     tGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCg                                                                                            <  2:2068844/101‑1 (MQ=255)
           ggcCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGGGCCGATGCGGTCTTCCCCGGGGCGCTGAGCTCGGTCGAGATGt                                                                                      >  1:3655940/1‑101 (MQ=255)
           ggcCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGGGCCGATGCGATCTTCCCCGAGGGGCTGACCTCGGGCggggagt                                                                                      >  2:747986/1‑95 (MQ=255)
             ccgccgAGGGGACCGGGGGGGCGGTGCCGCGGACGCATCTTTATGTCGAGGCGGACCCCAAGGGATTCTTCCCCGGGGCGCTGCCCTCGGTCGAGATGttc                                                                                    <  2:2091300/101‑1 (MQ=255)
              cgccgAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGGTCTTCCCCCCGGGGCTGGCCTCGGTCGAGAGGgtcc                                                                                   >  2:2917985/1‑101 (MQ=255)
                ccgAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgc                                                                                 <  1:660839/101‑1 (MQ=255)
                 cgAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGGGATCTTCCCCGGGGCGCGGGTCTCAGTCGAGGAGttgcgcg                                                                                >  1:453689/1‑101 (MQ=255)
                 cgAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgcg                                                                                >  2:718537/1‑101 (MQ=255)
                 cgAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgcg                                                                                <  1:1952825/101‑1 (MQ=255)
                 cgAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGACGCGATCTTCCCCGCGGGCCTTACCTCGGTCGAGGTGctccgcg                                                                                >  1:444250/1‑101 (MQ=255)
                  gAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGGGGTCTTCCCCCAGGCGCTGACCTCGGGCGGGATGttccgcgc                                                                               >  1:2790830/1‑101 (MQ=255)
                   aGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGGGCCGCTGCGATTTTTCCCCGGGGGCTGACCCCGGGCGGGGTGttccgcgcc                                                                              >  2:1448489/1‑101 (MQ=255)
                   aGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCCAGGCGCTGACCTCGGGCGAGATGttccgcgcc                                                                              >  2:3666251/1‑101 (MQ=255)
                     gggATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgcgcctt                                                                            <  2:4027006/101‑1 (MQ=255)
                     gggATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgcgcctt                                                                            <  2:2434454/101‑1 (MQ=255)
                      ggATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgcgccttc                                                                           >  1:2801200/1‑101 (MQ=255)
                      ggATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgcgccttc                                                                           <  2:205169/101‑1 (MQ=255)
                      ggATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGCGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgcgccttc                                                                           <  2:2267724/101‑1 (MQ=255)
                       gATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgcgccttcc                                                                          <  2:3077388/101‑1 (MQ=255)
                         tCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGttccgcgccttccgc                                                                        <  2:172258/101‑1 (MQ=255)
                            aGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCCGACCTCGGTCGAGATGttccgcgccttccgcgcc                                                                     >  2:226974/1‑101 (MQ=255)
                             gggggCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGccc                                                                    <  2:1421096/101‑1 (MQ=255)
                             gggggCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGccc                                                                    >  2:3984733/1‑101 (MQ=255)
                             gggggCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCCCGccc                                                                    >  1:926272/1‑101 (MQ=255)
                              ggggCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCg                                                                   <  2:2565590/101‑1 (MQ=255)
                               gggCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCgg                                                                  <  2:3665956/101‑1 (MQ=255)
                                    gTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGcc                                                             <  1:3409341/101‑1 (MQ=255)
                                          cgGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCggggg                                                       >  2:2928540/1‑100 (MQ=255)
                                           gggCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGGGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCCCGCCCGGCTGCCCgggggc                                                      >  1:873521/1‑99 (MQ=255)
                                           gggCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTTCGCGCCTTCCGCGCCCGGCTGCCCgggggc                                                      >  1:1207650/1‑99 (MQ=255)
                                           gggCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTc                                                      <  2:91479/101‑1 (MQ=255)
                                            ggCGCAGCTTTATGTCGATGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCTCCCGGCTGCCCGGGGTCa                                                     <  1:4192366/101‑1 (MQ=255)
                                            ggCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCa                                                     >  2:463165/1‑101 (MQ=255)
                                             gcgcAGCTTTATGTCGAGGCGGGCTCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCaa                                                    <  1:3683162/101‑1 (MQ=255)
                                             gcgcAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGGCCCGGGGCaa                                                    >  2:1930459/1‑101 (MQ=255)
                                              cgcAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAg                                                   <  1:2769370/101‑1 (MQ=255)
                                              cgcAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAg                                                   <  2:4170818/101‑1 (MQ=255)
                                                cAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCt                                                 <  1:1130658/101‑1 (MQ=255)
                                                cAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCt                                                 <  1:3545073/101‑1 (MQ=255)
                                                cAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCt                                                 <  1:2461429/101‑1 (MQ=255)
                                                cAGCTTTATGTCGAGGCGGGCGCCGATGCGATCCTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCt                                                 <  1:3603115/101‑1 (MQ=255)
                                                  gCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTc                                               <  2:3382472/101‑1 (MQ=255)
                                                   cTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCt                                              >  1:3092742/1‑101 (MQ=255)
                                                    tttATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTg                                             >  2:3793941/1‑101 (MQ=255)
                                                    tttATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGGCAAGCTTCTg                                             >  2:483134/1‑101 (MQ=255)
                                                    tttATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGGCAAGCTTCTg                                             >  1:3110695/1‑101 (MQ=255)
                                                    tttATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCCGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTg                                             >  2:2749977/1‑101 (MQ=255)
                                                     ttATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGGCAAGCTTCTgg                                            >  2:2858418/1‑101 (MQ=255)
                                                       aTGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGGCAAGCTTCTGGCg                                          >  2:2487941/1‑101 (MQ=255)
                                                         gTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCCGCCCCGGGTCAAGCTTCTGGCGaa                                        >  2:147051/1‑101 (MQ=255)
                                                          tCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAAc                                       <  1:374496/101‑1 (MQ=255)
                                                           cGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGGCAAGCTTCTGGGGAACa                                      >  1:3716390/1‑101 (MQ=255)
                                                            gAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACAt                                     <  2:1776101/101‑1 (MQ=255)
                                                            gAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACAt                                     <  2:1207556/101‑1 (MQ=255)
                                                            gAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACAt                                     <  1:27050/101‑1 (MQ=255)
                                                             aGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATg                                    >  2:3451054/1‑101 (MQ=255)
                                                             aGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATg                                    >  1:2439941/1‑101 (MQ=255)
                                                                cgggcgCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGAcc                                 >  1:1578838/1‑101 (MQ=255)
                                                                cgggcgCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGAcc                                 >  1:3967574/1‑101 (MQ=255)
                                                                cgggcgCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGAcc                                 <  2:1075315/101‑1 (MQ=255)
                                                                cgggcgCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGAcc                                 <  1:4042353/101‑1 (MQ=255)
                                                                cgggcgCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGAcc                                 <  1:1456274/101‑1 (MQ=255)
                                                                 gggcgCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCg                                <  2:803270/101‑1 (MQ=255)
                                                                  ggcgCCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGa                               >  1:4116179/1‑101 (MQ=255)
                                                                   gcgcCGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGaa                              <  2:2743585/101‑1 (MQ=255)
                                                                      ccGATGCGATCTTCCCCTAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTc                           >  1:839173/1‑101 (MQ=255)
                                                                      ccGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTc                           <  1:2858637/101‑1 (MQ=255)
                                                                      ccGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTc                           <  2:3858440/101‑1 (MQ=255)
                                                                      ccGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTc                           <  2:2800981/101‑1 (MQ=255)
                                                                      ccGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTc                           >  2:1676631/1‑101 (MQ=255)
                                                                      ccGATGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTc                           <  2:3651550/101‑1 (MQ=255)
                                                                      ccGATCCGGCCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTc                           <  1:510724/101‑1 (MQ=255)
                                                                         aTGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGc                        >  2:1119716/1‑101 (MQ=255)
                                                                         aTGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGc                        >  1:2237779/1‑101 (MQ=255)
                                                                          tGCGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGcc                       >  1:1105157/1‑101 (MQ=255)
                                                                            cGATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGc                     >  1:3956195/1‑101 (MQ=255)
                                                                             gATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCa                    <  2:735323/101‑1 (MQ=255)
                                                                             gATCTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCa                    <  1:3024328/101‑1 (MQ=255)
                                                                                cTTCCCCGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCAccc                 >  1:1629492/1‑101 (MQ=255)
                                                                                     ccGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGcc            >  2:608155/1‑101 (MQ=255)
                                                                                      cGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGccc           <  1:1930585/101‑1 (MQ=255)
                                                                                      cGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGccc           >  2:3744650/1‑101 (MQ=255)
                                                                                      cGAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGccc           <  2:2371134/101‑1 (MQ=255)
                                                                                       gAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCt          <  2:2446778/101‑1 (MQ=255)
                                                                                       gAGGCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGAACGAATTAGGCAGCACCCAGGCCCt          >  2:2029250/1‑101 (MQ=255)
                                                                                         ggCGCTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGa        >  1:1274844/1‑101 (MQ=255)
                                                                                          gcgcTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGAc       <  1:463222/101‑1 (MQ=255)
                                                                                          gcgcTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGAc       >  1:1074603/1‑101 (MQ=255)
                                                                                            gcTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGACcg     >  2:317867/1‑101 (MQ=255)
                                                                                            gcTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGACcg     >  2:2339693/1‑101 (MQ=255)
                                                                                            gcTGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGACcg     <  1:3984993/101‑1 (MQ=255)
                                                                                              tGACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGACcgcg   >  1:3029058/1‑101 (MQ=255)
                                                                                               gACCTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGACCGCgg  >  1:709206/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ACCGATGCGGCGGCCGCCGAGGGGATCGAGGGGGCGGTGGCGCGGGCGCAGCTTTATGTCGAGGCGGGCGCCGATGCGATCTTCCCCGAGGCGCTGACTTCGGTCGAGATGTTCCGCGCCTTCCGCGCCCGGCTGCCCGGGGTCAAGCTTCTGGCGAACATGACCGAATTCGGCCGCACCCCGGCCCTGACCGCGG  >  NC_014034/3541903‑3542098

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG