Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,596,459 C→G 100% G470G (GGC→GGG)  RCAP_rcc03391 → type I secretion system ATPase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,596,4590C→G100.0% 666.6 179G470G (GGC→GGG) RCAP_rcc03391type I secretion system ATPase
Reads supporting (aligned to +/- strand):  new base (95/84):  ref base (0/0):  total (95/84)

Only 100 of 196 total aligned reads displayed.

CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTCG  >  NC_014034/3596361‑3596556
                                                                                                  |                                                                                                 
cccgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcg                                                                                                 >  1:264889/1‑101 (MQ=255)
  cgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcggc                                                                                               <  1:3118254/101‑1 (MQ=255)
    agaTGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGctc                                                                                             >  2:2907352/1‑101 (MQ=255)
      atggtCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCtctc                                                                                           <  1:8971/101‑1 (MQ=255)
      atgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCtctc                                                                                           <  1:2042372/101‑1 (MQ=255)
        gatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgg                                                                                         <  1:2864270/101‑1 (MQ=255)
        gatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgg                                                                                         <  2:1151730/101‑1 (MQ=255)
         atCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGctcctggg                                                                                        >  2:3378398/1‑96 (MQ=255)
         atCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGGTCTcggg                                                                                        >  2:216450/1‑101 (MQ=255)
          tcgtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggc                                                                                       <  1:95693/101‑1 (MQ=255)
          tcgtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggc                                                                                       <  2:2357926/101‑1 (MQ=255)
            gtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcgg                                                                                     <  2:44628/101‑1 (MQ=255)
            gtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcgg                                                                                     >  1:757866/1‑101 (MQ=255)
              cgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCgggcgggc                                                                                   >  1:1311252/1‑101 (MQ=255)
               gATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTTTggggcggggg                                                                                  >  1:4028589/1‑99 (MQ=255)
               gATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCa                                                                                  <  1:1338061/101‑1 (MQ=255)
               gATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCa                                                                                  <  2:3205196/101‑1 (MQ=255)
                     gcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCa                                                                            <  2:1852445/101‑1 (MQ=255)
                       gAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgc                                                                          <  2:748995/101‑1 (MQ=255)
                        aaaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcg                                                                         >  2:2269600/1‑101 (MQ=255)
                        aaaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcg                                                                         >  2:1834025/1‑101 (MQ=255)
                        aaaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcg                                                                         <  2:3336527/101‑1 (MQ=255)
                         aaCGGGGGGCGGGGCAGCAGCGGATCCTCGATCTGCGCGAGGGATATGAAACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcgc                                                                        <  2:3646476/101‑1 (MQ=255)
                            gggCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTc                                                                     <  2:80990/101‑1 (MQ=255)
                                ggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGGAGATCCAGCGCGTCGGcc                                                                 >  1:2121156/1‑101 (MQ=255)
                                ggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGcc                                                                 <  2:421517/101‑1 (MQ=255)
                                ggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGcc                                                                 <  1:1048370/101‑1 (MQ=255)
                                 gcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCt                                                                <  2:3263392/101‑1 (MQ=255)
                                  cggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGGGGGCAGATCCAGCGCGTCGGCCTg                                                               >  2:490428/1‑101 (MQ=255)
                                  cggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTg                                                               >  2:600303/1‑101 (MQ=255)
                                   ggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCTGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTgg                                                              >  1:3721806/1‑101 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACCCCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGccc                                                           <  1:85500/101‑1 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGccc                                                           >  1:4174279/1‑101 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGccc                                                           <  1:191559/101‑1 (MQ=255)
                                       cATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGGGGGCGGCTCTCGGGGGGGCAGAGCCAGCGCGTCGGGCTGGGCCg                                                          >  1:1856231/1‑101 (MQ=255)
                                       cATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCg                                                          <  2:1929988/101‑1 (MQ=255)
                                        aTCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCgg                                                         >  2:3456820/1‑101 (MQ=255)
                                         tCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCCGGCCCggg                                                        >  2:1967810/1‑101 (MQ=255)
                                          cAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGgc                                                       >  2:3760569/1‑101 (MQ=255)
                                            gCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGgcgc                                                     <  2:639249/101‑1 (MQ=255)
                                             ttGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGCGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCt                                                    <  2:672569/100‑1 (MQ=255)
                                             cTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCt                                                    <  2:2636620/101‑1 (MQ=255)
                                             cTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCt                                                    >  2:830474/1‑101 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGGGCtt                                                   >  1:3904966/1‑101 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGCGCCGATCCAGCGCGGCGGCCTGGCCCCGGCGCta                                                   >  1:672646/1‑100 (MQ=255)
                                               gATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCttt                                                  <  1:2907571/101‑1 (MQ=255)
                                                aTCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCCGGCCCGGGCGCTTTa                                                 >  1:3442112/1‑101 (MQ=255)
                                                 tCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCtttcc                                                >  1:3460856/1‑99 (MQ=255)
                                                 tCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTAc                                                <  2:3001015/101‑1 (MQ=255)
                                                 tCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTAc                                                >  2:1685838/1‑101 (MQ=255)
                                                  ccTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCCGCGCGTCGGCCTGGCCCGGGCGCTTTACg                                               >  1:3231306/1‑101 (MQ=255)
                                                    tCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGc                                             <  1:1064948/101‑1 (MQ=255)
                                                    tCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGACCGGGCGCTTTACGGc                                             >  2:3128118/1‑101 (MQ=255)
                                                     cGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGcc                                            >  2:2422598/1‑100 (MQ=255)
                                                      gATCTGCCCGAGGGATATGACCCCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCaa                                           <  1:784345/101‑1 (MQ=255)
                                                      gATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGcga                                           >  1:3286469/1‑99 (MQ=255)
                                                      gATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGGaa                                           >  2:2562098/1‑101 (MQ=255)
                                                       aTCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAc                                          >  2:2826032/1‑101 (MQ=255)
                                                       aTCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCCCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGGCCGGGCGCTTTCCGGCCAc                                          >  1:516139/1‑101 (MQ=255)
                                                         cTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGGGCTTTACGGCAAacc                                        >  1:3538781/1‑101 (MQ=39)
                                                         cTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAccc                                        >  1:3167096/1‑101 (MQ=255)
                                                           gCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGGGCGTCGGCCTGGCCCGGGCGCTTTACCGCAAACCgg                                      >  2:2458688/1‑101 (MQ=255)
                                                           gCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCgg                                      <  1:723445/101‑1 (MQ=255)
                                                              cGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgc                                   <  1:2175711/101‑1 (MQ=255)
                                                              cGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgc                                   >  1:3709398/1‑101 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgct                                  >  1:3585689/1‑101 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgct                                  >  1:29542/1‑101 (MQ=255)
                                                                  ggATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTgctgct                               <  2:3148983/101‑1 (MQ=255)
                                                                   gATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGctgctg                              >  1:739384/1‑101 (MQ=255)
                                                                    atatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTgg                             >  2:537130/1‑101 (MQ=255)
                                                                    atatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTgg                             <  1:2784357/101‑1 (MQ=255)
                                                                    atatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGGCGGCCTGGCTCGGGCGCTTTACGGCAAACCGGTCCTGCTgg                             >  1:1382588/1‑101 (MQ=255)
                                                                     tatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGGGCTTTACGGCAACCCGGGGCTGCTggg                            >  1:1568936/1‑100 (MQ=255)
                                                                       tGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGc                          >  2:1883909/1‑101 (MQ=255)
                                                                         acacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTc                        <  2:2081719/101‑1 (MQ=255)
                                                                         acacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGGGCTGCTGGTGCTc                        >  2:3409892/1‑101 (MQ=255)
                                                                           acCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGGTcga                      >  1:1789983/1‑101 (MQ=255)
                                                                           acCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcga                      <  1:414598/101‑1 (MQ=255)
                                                                           acCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcga                      >  2:2409944/1‑101 (MQ=255)
                                                                           acCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGATGCTGGGGCTcgc                      >  1:2819279/1‑100 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGTTcgacg                    >  1:697319/1‑101 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacg                    <  2:739308/101‑1 (MQ=255)
                                                                               gggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAg                  <  1:3065367/101‑1 (MQ=255)
                                                                                ggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGc                 <  2:3721552/101‑1 (MQ=255)
                                                                                  tttCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCg               <  2:3928243/101‑1 (MQ=255)
                                                                                   ttCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGa              <  1:1008279/101‑1 (MQ=255)
                                                                                    tCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCCGATCCAGCGCGGCGGCCTGGCCCGGGCGCTTTAGGGCCACCCGGTGCTGCTGGGGCTCGGCGAGCCGaa             >  1:3808814/1‑101 (MQ=255)
                                                                                      gCAGGCGGGCGTGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATg           <  2:4028328/101‑1 (MQ=255)
                                                                                      gCAGGCGGGCGGGTGGCGCTCGGGCGCGCAGATCCAGCGCGTTGGCCTGGCCCGGGCGCGTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATg           <  2:1380911/101‑1 (MQ=255)
                                                                                      gCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATg           <  1:3760826/101‑1 (MQ=255)
                                                                                      gCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATg           <  1:1528179/101‑1 (MQ=255)
                                                                                      gCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATg           <  1:1480131/101‑1 (MQ=255)
                                                                                       cAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGc          <  1:42651/101‑1 (MQ=255)
                                                                                        aGGCGGGCGGGCCGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCg         >  1:2297237/1‑101 (MQ=255)
                                                                                         ggcgggcgGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGa        >  2:2972200/1‑101 (MQ=255)
                                                                                         ggcgggcgGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGa        <  2:3538528/101‑1 (MQ=255)
                                                                                           cgggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAt      <  1:2705284/101‑1 (MQ=255)
                                                                                           cgggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAt      >  1:1001150/1‑101 (MQ=255)
                                                                                            gggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtc     <  1:1212417/101‑1 (MQ=255)
                                                                                            gggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtc     <  2:390031/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTCG  >  NC_014034/3596361‑3596556

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG