Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,451 A→C 2.8% intergenic (+54/+94) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4510A→C 2.8% 4.3 ‑3.3 ‑0.0179intergenic (+54/+94)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (54/120):  total (58/121)
Fisher's exact test strand distribution p-value = 3.86e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.84e-01

Only 100 of 196 total aligned reads displayed.

GGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCC  >  NC_014034/432352‑432551
                                                                                                     |                                                                                                    
  ggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAgg                                                                                                    <  2:98066/101‑1 (MQ=255)
  ggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAgg                                                                                                    <  2:2229564/101‑1 (MQ=255)
   gatcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGCGGc                                                                                                   >  2:949985/1‑101 (MQ=255)
    atcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCa                                                                                                  <  2:1941438/101‑1 (MQ=255)
      cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTGAGGCAAg                                                                                                >  2:2281028/1‑101 (MQ=255)
       gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                                               >  1:3828010/1‑101 (MQ=255)
        atcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGAt                                                                                              <  2:350480/101‑1 (MQ=255)
        atcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGAt                                                                                              <  2:1224048/101‑1 (MQ=255)
          ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGa                                                                                            <  1:3948391/101‑1 (MQ=255)
          ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGa                                                                                            <  1:1867155/101‑1 (MQ=255)
          ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGa                                                                                            <  2:1754422/101‑1 (MQ=255)
           cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaa                                                                                           <  1:3177608/101‑1 (MQ=255)
           cgcgCACGCTTTCGGCGGCGGCGGGACACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGATATTTGAGGCAAGATCaa                                                                                           >  2:209752/1‑101 (MQ=21)
            gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                                          <  2:1022629/101‑1 (MQ=255)
             cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAg                                                                                         >  2:1096199/1‑101 (MQ=255)
             cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAg                                                                                         <  1:4096283/101‑1 (MQ=255)
             cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAg                                                                                         <  2:325088/101‑1 (MQ=255)
               cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                       <  2:1456843/101‑1 (MQ=255)
               cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                       <  2:1820617/101‑1 (MQ=255)
               cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                       <  1:450445/101‑1 (MQ=255)
                aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAACCg                                                                                      >  1:2116860/1‑101 (MQ=255)
                   cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                                   <  1:1036014/101‑1 (MQ=255)
                    tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                  <  2:805331/101‑1 (MQ=255)
                    tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                  <  2:3372628/101‑1 (MQ=255)
                     ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                 <  2:3461817/101‑1 (MQ=255)
                     ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                 <  1:4129277/101‑1 (MQ=255)
                      tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                >  1:2700722/1‑101 (MQ=255)
                        ggcggcggcggGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGc                                                                              <  1:2476486/101‑1 (MQ=255)
                        ggcggcggcggGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGc                                                                              <  1:1715165/101‑1 (MQ=255)
                        ggcggcggcggGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGc                                                                              <  1:1128122/101‑1 (MQ=255)
                         gcggcggcggGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                             <  2:2576130/101‑1 (MQ=255)
                          cggcggcggGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                            >  1:3996503/1‑101 (MQ=255)
                            gcggcggGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                          <  2:2557397/101‑1 (MQ=255)
                             cggcggGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                         >  1:2825825/1‑101 (MQ=255)
                              ggcggGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGACGATCCGGGCTTCGtt                                                                        >  2:185795/1‑101 (MQ=255)
                                  ggCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                    <  2:3337342/101‑1 (MQ=255)
                                  ggCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                    <  1:2273567/101‑1 (MQ=255)
                                  ggCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCAGGATATTTGAGGCAAGATGAAAGACGATCCGGGCTTCGTTTCAt                                                                    >  1:535294/1‑101 (MQ=21)
                                    ccACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                  <  1:2588934/101‑1 (MQ=255)
                                     cACGCCTGAGGCCGGGCAGGGGGGGGGGGGCCCCCCCC.CCCCGGGCTCCCCCCCCGGTATTTTTGGCAAAAAGAAAGACGATCNGGGGCTCGTTTCTTCtt                                                                 >  2:123748/1‑101 (MQ=255)
                                         ccTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGGCTCCCCCCCGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTTccc                                                             >  1:1294958/1‑101 (MQ=21)
                                         ccTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGccc                                                             <  2:2199025/101‑1 (MQ=255)
                                          cTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGCGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                            <  2:2158909/101‑1 (MQ=255)
                                           tGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                           <  2:3900060/101‑1 (MQ=255)
                                              ggCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCTGGCTTCGTTTCATCTTGCCCCAAat                                                        <  2:2438789/101‑1 (MQ=255)
                                              ggCCGGACG.GCGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                        <  2:1521615/101‑1 (MQ=21)
                                                  gggCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                    <  2:1646821/101‑1 (MQ=255)
                                                   ggCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATccc                                                   <  1:3282418/101‑1 (MQ=255)
                                                    gga.GGGGGGCGCCTCCCCCCGTCCTGCGGGCCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                  <  1:3344326/99‑1 (MQ=25)
                                                    gCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                  >  2:2374861/1‑101 (MQ=255)
                                                     cA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgg                                                 <  1:807968/101‑1 (MQ=255)
                                                     cA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgg                                                 >  2:3416748/1‑101 (MQ=255)
                                                        ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                               <  1:2554840/101‑1 (MQ=255)
                                                          ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                                             <  1:193206/101‑1 (MQ=255)
                                                             gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                                          <  1:4067138/101‑1 (MQ=255)
                                                               gcTGCCTCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                        <  1:2398561/101‑1 (MQ=255)
                                                               gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                        <  1:2000246/101‑1 (MQ=255)
                                                               gcTCCCCCCCGTCCGGCGGACGCCCCCCGGGATATGTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                        <  1:750468/101‑1 (MQ=25)
                                                                cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                       <  2:3176689/101‑1 (MQ=255)
                                                                 tGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTTTAGCAAAAATAAAACCCATCCCGGGTTCCTTTTTTCTTTCCCCCAAAATACCggggggggaattt                                      >  2:811219/1‑93 (MQ=0)
                                                                 tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                      >  1:2785498/1‑101 (MQ=255)
                                                                 tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                      <  2:668546/101‑1 (MQ=255)
                                                                  gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                     <  1:3713120/101‑1 (MQ=255)
                                                                  gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                     >  1:1827773/1‑101 (MQ=255)
                                                                    cccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgc                                   <  1:362820/101‑1 (MQ=255)
                                                                     ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcg                                  >  2:1896743/1‑101 (MQ=255)
                                                                       ccgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCACa                                <  1:470333/96‑1 (MQ=255)
                                                                         gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcagatc                              >  2:579746/4‑96 (MQ=255)
                                                                         gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                              <  2:3668158/98‑1 (MQ=255)
                                                                         gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                              >  1:310213/4‑101 (MQ=255)
                                                                         gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGAGGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                              <  1:163062/98‑1 (MQ=255)
                                                                          tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                             >  1:322200/3‑101 (MQ=255)
                                                                           ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                            >  2:1830125/2‑101 (MQ=255)
                                                                           ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                            >  2:2893499/2‑101 (MQ=255)
                                                                             tGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                          <  1:3689212/101‑1 (MQ=255)
                                                                              gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                         >  1:2646921/1‑101 (MQ=255)
                                                                              gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCACGGGGGTGAATTTTGTCGCAGCCaaaa                         >  1:2726006/1‑101 (MQ=255)
                                                                                ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                       <  2:3827753/101‑1 (MQ=255)
                                                                                ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                       >  2:3124733/1‑101 (MQ=255)
                                                                                 gACTCGCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                      <  2:2784951/101‑1 (MQ=255)
                                                                                  aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                     <  1:1391356/101‑1 (MQ=255)
                                                                                   cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggg                    >  2:1688248/1‑101 (MQ=255)
                                                                                   cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggg                    <  1:612572/101‑1 (MQ=255)
                                                                                    tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                   >  1:3470084/1‑101 (MQ=255)
                                                                                      cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                 <  2:566316/101‑1 (MQ=255)
                                                                                       ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                <  1:2281196/101‑1 (MQ=255)
                                                                                         ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc              >  1:3273064/1‑101 (MQ=255)
                                                                                           gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc            >  1:2560890/1‑101 (MQ=255)
                                                                                              atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc         <  2:892674/101‑1 (MQ=255)
                                                                                                atTTGAGGTAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTCGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt       >  1:1240958/1‑101 (MQ=255)
                                                                                                atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt       <  2:3265608/101‑1 (MQ=255)
                                                                                                 tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa      >  2:3294826/1‑101 (MQ=255)
                                                                                                 tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa      <  2:1325646/101‑1 (MQ=255)
                                                                                                 tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa      >  1:147723/1‑101 (MQ=255)
                                                                                                 tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa      <  1:1096293/101‑1 (MQ=255)
                                                                                                  ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt     >  2:1177203/1‑101 (MQ=255)
                                                                                                   tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc    >  1:868139/1‑101 (MQ=255)
                                                                                                    gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc   >  1:2685378/1‑101 (MQ=255)
                                                                                                    gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc   >  1:2431483/1‑101 (MQ=255)
                                                                                                     aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc  >  2:4183578/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCA.GGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCC  >  NC_014034/432352‑432551

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG