Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 444,483 C→G 100% L51L (CTG→CTC RCAP_rcc00416 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034444,4830C→G100.0% 696.8 185L51L (CTG→CTCRCAP_rcc00416LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (86/99):  ref base (0/0):  total (86/99)

Only 100 of 193 total aligned reads displayed.

TCATGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCCAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCGATGG  >  NC_014034/444385‑444580
                                                                                                  |                                                                                                 
  aTGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGc                                                                                               >  1:1983091/1‑101 (MQ=255)
   tGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCt                                                                                              <  1:2624315/101‑1 (MQ=255)
    gCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTg                                                                                             >  2:3322161/1‑101 (MQ=255)
     cAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTgg                                                                                            <  1:2997887/101‑1 (MQ=255)
     cAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTgg                                                                                            >  2:583373/1‑101 (MQ=255)
     cAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTgg                                                                                            <  2:465787/101‑1 (MQ=255)
       ggCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGtt                                                                                          <  2:3498938/101‑1 (MQ=255)
          cAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTcc                                                                                       >  1:121878/1‑101 (MQ=255)
           aaGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCa                                                                                      <  1:34014/101‑1 (MQ=255)
           aaGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCa                                                                                      >  2:1450088/1‑101 (MQ=255)
           aaGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCa                                                                                      <  2:3029757/101‑1 (MQ=255)
           aaGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCa                                                                                      <  2:311199/101‑1 (MQ=255)
            aGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAg                                                                                     >  2:1913711/1‑101 (MQ=255)
              gATGCGGGCGGCGTAATCGAGCAGTTCCGCNCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAgcc                                                                                   <  1:664341/101‑1 (MQ=255)
               aTGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGCGGATGGGTTCCCgccg                                                                                  >  2:690933/1‑101 (MQ=255)
                  cgggcggCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGTCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGccgccg                                                                               <  2:813042/101‑1 (MQ=255)
                  cgggcggCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGccgccg                                                                               <  1:1566474/101‑1 (MQ=255)
                  cgggcggCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGccgccg                                                                               <  2:2712756/101‑1 (MQ=255)
                  cgggcggCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGccgccg                                                                               >  2:223904/1‑101 (MQ=255)
                   gggcggCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCgg                                                                              <  1:3273834/101‑1 (MQ=255)
                   gggcggCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCgg                                                                              <  2:4004648/101‑1 (MQ=255)
                   gggcggCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCgg                                                                              <  1:1123042/101‑1 (MQ=255)
                    ggcggcGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGa                                                                             >  1:2063271/1‑101 (MQ=255)
                     gcggcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGAt                                                                            <  1:95772/101‑1 (MQ=255)
                      cggcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATc                                                                           <  1:3171011/101‑1 (MQ=255)
                      cggcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCCCCCGATc                                                                           >  2:405914/1‑101 (MQ=255)
                            aaTCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTgg                                                                     <  2:3680068/101‑1 (MQ=255)
                            aaTCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTgg                                                                     >  2:902820/1‑101 (MQ=255)
                            aaTCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGATGGTTTCCAGCCGCCGGAGCTTTTgg                                                                     >  1:3552998/1‑101 (MQ=255)
                             aTCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGc                                                                    >  2:4209772/1‑101 (MQ=255)
                               ccaccAGTCCCTCGCCCGCGGCGGTGAACAGCGGCCCGCCGGCGCCGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCTTATCTTGTGGCTg                                                                  <  2:4157439/97‑1 (MQ=255)
                               cGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTg                                                                  <  2:2659663/101‑1 (MQ=255)
                               cGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTg                                                                  >  2:1293611/1‑101 (MQ=255)
                               cGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTg                                                                  <  1:2202203/101‑1 (MQ=255)
                               cGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTg                                                                  <  1:1089349/101‑1 (MQ=255)
                                 aGCAGTCCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGAc                                                                <  1:2871736/101‑1 (MQ=255)
                                  gCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACg                                                               <  2:843560/101‑1 (MQ=255)
                                    aGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGc                                                             >  1:2350156/1‑101 (MQ=255)
                                     gTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGcc                                                            <  2:2998361/101‑1 (MQ=255)
                                      ttCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCg                                                           <  2:1547866/101‑1 (MQ=255)
                                      ttCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCg                                                           <  2:2063133/101‑1 (MQ=255)
                                      tcccGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCg                                                           <  1:1501770/99‑1 (MQ=255)
                                         cgcgCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGAtt                                                        <  1:416028/101‑1 (MQ=255)
                                         cgcgCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGAtt                                                        <  1:3322402/101‑1 (MQ=255)
                                          gcgcCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTg                                                       <  2:3082708/101‑1 (MQ=255)
                                           cgcCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGc                                                      <  1:325652/101‑1 (MQ=255)
                                           cgcCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGc                                                      >  2:2383643/1‑101 (MQ=255)
                                            gcCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCg                                                     <  2:3542398/101‑1 (MQ=255)
                                              ccGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTc                                                   <  1:2142455/101‑1 (MQ=255)
                                              ccGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTc                                                   >  1:3063228/1‑101 (MQ=255)
                                               cgcgGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCa                                                  <  1:1239936/101‑1 (MQ=255)
                                                 cggcggTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCggtagg                                                >  2:198597/1‑96 (MQ=255)
                                                 cggcggTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAgg                                                >  2:2785722/1‑101 (MQ=255)
                                                  ggcggTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCGGGATCTTGTGGCTGACGGCCGATTGCGTCAGGc                                               >  1:3604342/1‑101 (MQ=255)
                                                  ggcggTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGGCAGGc                                               >  1:2238850/1‑101 (MQ=255)
                                                   gcggTGAAGAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGcc                                              <  2:2450881/101‑1 (MQ=255)
                                                    cggTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGccc                                             <  1:2969567/101‑1 (MQ=255)
                                                     ggTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCa                                            >  1:1735807/1‑101 (MQ=255)
                                                     ggTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCa                                            >  2:262569/1‑101 (MQ=255)
                                                       tGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCaaa                                          <  2:3047667/101‑1 (MQ=255)
                                                       tGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCaaa                                          <  1:583437/101‑1 (MQ=255)
                                                         aaCAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGc                                        >  1:4145986/1‑101 (MQ=255)
                                                            aGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGcccc                                     >  1:4183309/1‑99 (MQ=255)
                                                            aGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTc                                     <  2:1811638/101‑1 (MQ=255)
                                                             gCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTcg                                    <  2:3427320/101‑1 (MQ=255)
                                                              cGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGGCGGTTGCGTCAGGGCCCAAGCCTcgg                                   >  2:2283771/1‑101 (MQ=255)
                                                              cGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTcgg                                   <  2:3232381/101‑1 (MQ=255)
                                                              cGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTcgg                                   <  2:2885603/101‑1 (MQ=255)
                                                               ggCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTcggc                                  <  1:150447/101‑1 (MQ=255)
                                                               ggCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTcggc                                  <  1:3534005/101‑1 (MQ=255)
                                                               ggCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGAGGCAGGCCCAAAGGCTcggc                                  >  2:3850480/1‑101 (MQ=255)
                                                               ggCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCCATTGCGTCAGGCCCAAAggccccgc                                  >  2:3345290/1‑94 (MQ=255)
                                                                 cccGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTcggcgg                                <  1:2177268/101‑1 (MQ=255)
                                                                      cgGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCgg                           <  2:3975295/101‑1 (MQ=255)
                                                                         ggcggcGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTg                        <  1:1859320/101‑1 (MQ=255)
                                                                         ggcggcGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTg                        <  1:3186695/101‑1 (MQ=255)
                                                                          gcggcgCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGa                       <  1:3262811/101‑1 (MQ=255)
                                                                           cggcgCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCggggggg                      >  1:92473/1‑97 (MQ=255)
                                                                            ggcgCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGGCGCCGGGTCTTGTGGCTGGCGGCCGGTTGCGGCAGGCCCCAAGCCCCGGGGGGCCgggggggg                     >  2:1489393/1‑96 (MQ=255)
                                                                              cgcgACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGt                   <  2:961120/101‑1 (MQ=255)
                                                                               gcgACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTg                  <  1:983929/101‑1 (MQ=255)
                                                                                cgACAGAAGATCCGCGCCGAGGCTGGTTTCCAGGCGCCGGATCTTGTGGCCGACGGCCGATCGCGTCAGGCCCAAAGCCTCGGGGGCGCGGGGGACCGttc                 >  1:2808925/1‑99 (MQ=255)
                                                                                cgACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGc                 <  2:674303/101‑1 (MQ=255)
                                                                                  aCAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCg               >  2:727393/1‑101 (MQ=255)
                                                                                  aCAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCg               <  1:902905/101‑1 (MQ=255)
                                                                                   cAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGt              <  1:4036522/101‑1 (MQ=255)
                                                                                   cAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGGGCCgg              >  1:1271090/1‑100 (MQ=255)
                                                                                   cAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCAGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGt              <  1:3850739/101‑1 (MQ=255)
                                                                                    agaagaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGTGCCGTg             >  1:2568239/1‑101 (MQ=255)
                                                                                     gaagaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGGGCCGCGa            >  1:428787/1‑101 (MQ=255)
                                                                                      aagaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGCCTGACGGCCGATTGCGTGAGGCCCAAAACCTCCGCGGCGCGGGGGACGGGGCCGTGAt           >  2:2159328/1‑101 (MQ=255)
                                                                                         aTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGGGCGGGGGACGGGGGCGGGGTggg        >  1:3537166/1‑101 (MQ=255)
                                                                                         aTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATggg        <  1:690488/101‑1 (MQ=255)
                                                                                         aTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATggg        <  2:3152743/101‑1 (MQ=255)
                                                                                         aTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGGGCCGTGATggg        >  2:3759802/1‑101 (MQ=255)
                                                                                           ccGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCg      <  2:2984162/101‑1 (MQ=255)
                                                                                              cgcCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCGATg   <  1:1304975/101‑1 (MQ=255)
                                                                                              cgcCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCGATg   <  2:1612091/101‑1 (MQ=255)
                                                                                              cgcCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTCACGGTGCCGTGATGGGCGATg   <  1:2403786/101‑1 (MQ=255)
                                                                                               gcCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGGGCCGTGATGGGCGATgg  >  2:1723137/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TCATGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCCAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCGATGG  >  NC_014034/444385‑444580

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG