Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 448,780 A→C 4.3% intergenic (+73/+147) RCAP_rcc00419 → / ← pykA1 NADH:flavin oxidoreductase/NADH oxidase/pyruvate kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034448,7800A→C 4.3% 5.0 ‑0.4 ‑0.797intergenic (+73/+147)RCAP_rcc00419/pykA1NADH:flavin oxidoreductase/NADH oxidase/pyruvate kinase
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (56/36):  total (60/37)
Fisher's exact test strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.79e-01

Only 100 of 139 total aligned reads displayed.

TGCGCTGCGGCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCTGTTGTGCTTGGGGCAGGCGGTCGTGCCG  >  NC_014034/448680‑448874
                                                                                                      |                                                                                                 
ttggcTGCGGGTGGTGAAGGACGTTTGAGGCTTACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.Ca                                                                                                   <  1:1234386/98‑1 (MQ=38)
 gcgcTGCGGCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CAc                                                                                                  <  2:3567571/101‑1 (MQ=255)
   gcTGCGGCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTc                                                                                                >  2:1657066/1‑101 (MQ=255)
    cTGCGGCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCg                                                                                               <  2:2392599/101‑1 (MQ=255)
     tGCGGCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCgg                                                                                              >  2:2725462/1‑101 (MQ=255)
     tGCGGCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCgg                                                                                              <  2:777101/101‑1 (MQ=255)
       cGGCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.cc                                                                                           >  2:63829/1‑101 (MQ=255)
       cGGCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.cc                                                                                           <  1:1490525/101‑1 (MQ=255)
       cGGCTAATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.cc                                                                                           <  2:110670/101‑1 (MQ=255)
        ggCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCt                                                                                          >  2:4056636/1‑101 (MQ=255)
         gCTGATGAAGGCCGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTg                                                                                         <  2:884883/101‑1 (MQ=255)
         gCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTg                                                                                         <  1:3251722/101‑1 (MQ=255)
         gCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTg                                                                                         <  2:840609/101‑1 (MQ=255)
          cTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGGGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTgc                                                                                        <  1:868274/101‑1 (MQ=255)
          cTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTgc                                                                                        <  2:3744501/101‑1 (MQ=255)
           tgatgaAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTgcg                                                                                       <  1:3097246/101‑1 (MQ=255)
            gatgaAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTgcgc                                                                                      >  2:938548/1‑101 (MQ=255)
             atgaAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGcgcc                                                                                     <  1:4100939/101‑1 (MQ=255)
             atgaAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATAGCAACAGAGGTACTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.ccgaaacc                                                                                     >  1:1169554/1‑95 (MQ=38)
              tgaAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGcgccc                                                                                    >  2:1160921/1‑101 (MQ=255)
               gaAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGcgcccg                                                                                   <  1:1632013/101‑1 (MQ=255)
               gaAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGcgcccg                                                                                   <  2:3400326/101‑1 (MQ=255)
               gaAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGcgcccg                                                                                   >  1:4144913/1‑101 (MQ=255)
                  ggACGTTGGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCgcccgccc                                                                                <  2:2199512/101‑1 (MQ=255)
                   gccGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGcccgcccc                                                                               <  1:3692845/99‑1 (MQ=255)
                   gACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGcccgcccc                                                                               >  1:1078532/1‑101 (MQ=255)
                   gACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGcccgcccc                                                                               >  1:3377567/1‑101 (MQ=255)
                    aggTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGcccgccccg                                                                              <  2:4166093/99‑1 (MQ=255)
                     cGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGcccgccccgc                                                                             <  2:2654325/101‑1 (MQ=255)
                     cGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGcccgcccccc                                                                             >  2:945760/1‑99 (MQ=255)
                      gTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCccgccccgcc                                                                            >  2:1953455/1‑101 (MQ=255)
                      gTTTGAGGCTGACTTGCCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCccgccccgcc                                                                            <  2:3976850/101‑1 (MQ=255)
                       tttGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGCCCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCa                                                                           <  1:1561817/101‑1 (MQ=255)
                       tttGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCa                                                                           >  1:2454037/1‑101 (MQ=255)
                        ttGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCAt                                                                          >  2:1314652/1‑101 (MQ=255)
                        ttGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCAt                                                                          >  2:416467/1‑101 (MQ=255)
                         tGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATc                                                                         <  2:79136/101‑1 (MQ=255)
                         tGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATc                                                                         <  2:68796/101‑1 (MQ=255)
                           aGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATcgg                                                                       >  1:2344726/1‑101 (MQ=255)
                           aGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATcgg                                                                       >  1:2097003/1‑101 (MQ=255)
                             gCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATcggcg                                                                     >  1:3696866/1‑101 (MQ=255)
                               tGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCggcggg                                                                   >  1:982153/1‑101 (MQ=255)
                                gACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCggcgggg                                                                  >  1:2978436/1‑100 (MQ=255)
                                  ctggcccTCGGCCATTGCCGAGCCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGCCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGgcgc                                                                <  2:1995102/96‑1 (MQ=255)
                                  cTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCCTCggcgggggg                                                                >  2:1973642/1‑98 (MQ=255)
                                   ttGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCTGgggcc                                                               >  2:1744074/1‑100 (MQ=255)
                                   ttGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGGCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCCCCCGCCCCGCCATCggccggccgc                                                               >  2:1372932/1‑98 (MQ=25)
                                   tgggCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGCCCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCa                                                               <  1:1769026/99‑1 (MQ=255)
                                   tgggCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCa                                                               <  2:3633192/99‑1 (MQ=255)
                                   tggcccccGGCCTTGGCGGGGGCCAACCGCAACGGAGGTCTGGTGCCCGCCGGGGGCCCAAG.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCa                                                               <  1:2256554/94‑1 (MQ=9)
                                    tGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCggcgggggggg                                                              >  1:1062596/1‑96 (MQ=255)
                                    tGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCaa                                                              >  1:3002705/1‑100 (MQ=255)
                                     ggCCTCGGCCATTGCCGGGGCCAATCGCAACAGAGGTCCTGTGCCCGCCGGGGGCCCAAA.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGt                                                             <  1:3059284/101‑1 (MQ=18)
                                      gcccgggCCTTTGCCGAGCCCAACCCAAACGGGGGTCCTGTGCCCGCCGAGGGCCCAAA.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTc                                                            <  2:1663265/96‑1 (MQ=14)
                                      gCCTCGGCCATTGCCGAGGCAAATCGCAACGGGGTTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTc                                                            <  2:3358773/101‑1 (MQ=21)
                                       ccccGGCCTTTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCAAAA.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTc.g                                                          <  1:757588/98‑1 (MQ=18)
                                       ccccGGCCATTGCCGAGCCAAATCGCAACAGAGGTCCTGTGCCCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTc.g                                                          <  2:829803/98‑1 (MQ=21)
                                       ccTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCggcggggggggggg                                                           >  1:3213369/1‑93 (MQ=255)
                                       ccTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGgggcgggcg                                                           >  1:359755/1‑96 (MQ=255)
                                        cTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCggcggggggccgggc                                                          >  1:709535/1‑92 (MQ=255)
                                         tCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCggcggggggcggggcg                                                         >  2:2999835/1‑91 (MQ=255)
                                          cGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGgggcgggggggc                                                        >  1:1785582/1‑93 (MQ=255)
                                          cGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGGAGGC.gc.gc                                                      >  2:2327657/1‑101 (MQ=255)
                                            gCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.Gc.gccg                                                    <  2:2518402/101‑1 (MQ=255)
                                             ccATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGGGGGC.Gc.gccgc                                                   >  2:2873342/1‑101 (MQ=25)
                                                   ccGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGGGGGC.GC.GCCGCCcgggg                                             >  1:3709648/1‑100 (MQ=21)
                                                    cGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGGGGGC.GC.gcccccccgggg                                            >  1:3006396/1‑95 (MQ=18)
                                                     ggggCCAACCGCAACGGGGGTCCTGTGCCCGCGGGGGGCCCAAG.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCgg                                           <  1:3359733/99‑1 (MQ=14)
                                                     gAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCGGGC.GC.gcccccccggggg                                           >  2:4038686/1‑94 (MQ=21)
                                                      aGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGGGGGG.GC.CCCGCCCGGGCggg                                          >  1:1235503/1‑100 (MQ=18)
                                                       ggCAAATGGCAACAGGGGTCCGGGGACCGCCGAGGCCCCAAG.GG.CACTCGC.CCTGCCCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAg                                         <  1:1575132/101‑1 (MQ=17)
                                                        gCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCCGGCGGC.GCCCCCggggggcgg                                         >  1:2228573/1‑96 (MQ=18)
                                                         ccAACCGCAACAGGGGTCCTGTGCCCCCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAgcg                                       <  1:1984440/101‑1 (MQ=17)
                                                         ccAACCGAAACGGGGGTCCTGTGCCCGCCGAGGCCCCAAA.GC.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAgcg                                       <  2:2920060/101‑1 (MQ=14)
                                                          cAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCAGGC.GC.GCCCCCCGGGGGGGgcgc                                      >  1:1705751/1‑101 (MQ=18)
                                                             ccGCAACGGAGGTCCGGTGCCCGCCGAGGGCCCAAA.GG.CACGCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAgg                                   <  1:3780677/100‑1 (MQ=17)
                                                               caagcgggggTCGTGTGCCCGCCGAGGCCCCAAA.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGcc                                 <  2:1687442/93‑1 (MQ=11)
                                                                aaaCAGAGGCCGTGTGCCCGCCG.GGGCCCAAAGGG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCct                                <  2:2892451/100‑1 (MQ=14)
                                                                 accgggggTCCTGGGCCCGCCGGGGGCCAAAG.GC.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCctc                               <  1:434935/95‑1 (MQ=14)
                                                                   cggggtcccTGTGCCCGCCGAGGGCCAAAA.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCtg                             <  2:3682363/94‑1 (MQ=11)
                                                                    aggggTCGTGTGCCCGCGGGGGGCCCAAG.GC.CATTGGG.CGTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCtgt                            <  1:1400201/98‑1 (MQ=14)
                                                                    agaaGTCCCGTGACCGCCGGGGGCCCAAA.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCGGAG.GCGGCCCCCGGGCCGGGgcggcgggcccgc                             >  2:2115876/1‑91 (MQ=14)
                                                                        ggccTGGGCCCCCCGGGGGCCCCAA.GG.CCCCCGGCCCGGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCtgttgt                         <  1:2910055/99‑1 (MQ=11)
                                                                         tCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCACCCGCCCCGCCATCGGCGGGGGCGGGG.GCGGCCGCCGGGGGGGGGGGCGGGccccgtttttc                        >  2:1055170/1‑92 (MQ=9)
                                                                            tgtgACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGGAGGC.GC.GCCGCCCGGGGGGGGGGCCGGCCCCCGTTGGGCTTg                    >  1:564101/1‑101 (MQ=14)
                                                                            tgtgACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCGGGC.GG.GCCCCCGGGGGGGGGGGCAGGGCCCCGTTGTGTTTg                    >  2:452996/1‑101 (MQ=14)
                                                                               gACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGGCGGC.GC.GCCGCCCCGGGGGGGGGGGGGGCCCCGTTGTGGTTgggg                 >  2:1396/1‑101 (MQ=2)
                                                                               gACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCCGGC.GC.GCCCCCCGGGGGGGGGGGGGGCCCCCGTTGTGCTTtggg                 >  1:2040424/1‑101 (MQ=9)
                                                                                cccgccgAGGGCCCAAG.GG.CACCCGG.CCTGCCCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCTGTTGTGCTTGGGGc                <  1:1055264/100‑1 (MQ=255)
                                                                                 cccgcgggggCCCAAAGGG.CACCCGC.CCGGCCCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCTGTTGTGCTTGGGGc                <  1:3876431/94‑1 (MQ=14)
                                                                                 ccagcgAGGGCAGAAA.GG.CAGTGGG.CCGGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCTGTTGTGCTTGGGGCa               <  2:3308583/97‑1 (MQ=14)
                                                                                   ggcgAGGGCCCAAA.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCCGTC.GC.GCCGCCCGGGGGGGGGGCGGGGCCCTGGTCTGCTTtgggggac             >  1:2836779/3‑97 (MQ=2)
                                                                                   cccgGGGGCCCAAA.GGCCCCCCGG.CCTGCCCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCTGTTGTGCTTGGGGCAg              <  1:3000057/100‑1 (MQ=11)
                                                                                    ccgAGGGCCCAAA.GG.CCCTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCAATC.CG.GCCGCCCGCGCGGGGGGGCCGCCTCTTTTGTGCCTcggggtgga            >  1:4156085/1‑96 (MQ=2)
                                                                                    ccgAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGGCGGC.GC.GCCCCCCGGGGGGGGGGCCGGGCCCCGTTGTTCTTTGGGGAggg            >  2:3717344/1‑100 (MQ=9)
                                                                                     cgAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCGGGC.GC.GCCGCCGGGGCGGGGGGCCGGGCCCTGTTGTGGTTtggggggggg           >  1:3273965/1‑95 (MQ=11)
                                                                                       aGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCCGGC.GC.CCCCCCCGGGGGGGGGGCCGGGCCCCCTTTTGCTTgggggggggggg         >  1:3957797/1‑93 (MQ=2)
                                                                                        gggCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGCAGGC.GC.CGCCCCCGGGCGGGGGGCGGGGCCCCGTTCTTCTTGTGGCAggggcgc        >  1:3859423/1‑96 (MQ=9)
                                                                                        ggccccAAG.GG.CGCCCGG.CGCGCCCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCTGTTGTGCTTGGGGCAGGCGGTc        <  1:1361768/98‑1 (MQ=17)
                                                                                              aaa.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGGGGCGGC.GC.GCCCCCCGGGGGGGGGGGGGGCCCCCGCTGTGGTTTGGGGGGGCggggggggcg  >  1:2403168/1‑93 (MQ=2)
                                                                                                      |                                                                                                 
TGCGCTGCGGCTGATGAAGGACGTTTGAGGCTGACTTGGCCTCGGCCATTGCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAA.GG.CACTCGG.CCTGCGCCCGCCCCGCCATCGGCGGGCGCAGTC.GC.GCCGCCGGGGCGGAGCGCAGGCCTCTGTTGTGCTTGGGGCAGGCGGTCGTGCCG  >  NC_014034/448680‑448874

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG