Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 530,946 T→C 100% intergenic (+24/+117) valS → / ← RCAP_rcc00497 valyl‑tRNA synthetase/UbiA prenyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034530,9460T→C100.0% 307.8 117intergenic (+24/+117)valS/RCAP_rcc00497valyl‑tRNA synthetase/UbiA prenyltransferase
Reads supporting (aligned to +/- strand):  new base (43/71):  ref base (0/0):  total (44/73)

Only 100 of 119 total aligned reads displayed.

GAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..C.CGCGCTTGTCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530869‑531022
                                                                                |                                                                            
 aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATAttt                                                       >  1:1974813/1‑101 (MQ=25)
 aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATAttt                                                       >  1:3160480/1‑101 (MQ=25)
  aggggcggcGAAGATGGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATAtttt                                                      <  2:2959473/98‑1 (MQ=21)
  agagGCGGCGATGATCGGCGTGGCGCTGAGGCGTCTGGCCGATCTGGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATAtttt                                                      <  1:126325/101‑1 (MQ=16)
  agagGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATAtttt                                                      <  2:607751/101‑1 (MQ=25)
    agGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCg                                                    >  1:2456427/1‑101 (MQ=25)
    agGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCg                                                    <  2:2078966/101‑1 (MQ=25)
    agGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCg                                                    <  1:1119140/101‑1 (MQ=25)
      ggggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGc                                                  <  1:3014581/99‑1 (MQ=21)
       cggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCa                                                 <  2:3827614/101‑1 (MQ=25)
       cggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCa                                                 <  2:3052313/101‑1 (MQ=25)
        ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCaa                                                <  2:664889/101‑1 (MQ=25)
         gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAg                                               <  1:557607/101‑1 (MQ=25)
          cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  2:3681422/101‑1 (MQ=21)
          cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  2:3823317/101‑1 (MQ=21)
           gAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGAt                                             <  1:4013961/101‑1 (MQ=21)
           gAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGAt                                             >  1:3046871/1‑101 (MQ=21)
           gAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGAt                                             >  1:1221764/1‑101 (MQ=21)
            aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  2:1819172/101‑1 (MQ=21)
             aGATCGGCGCGGGCCGGAAGCGTCTGCCCGAATTAGGATAAAGGAAGAAGGGGGGCTCG.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaa                                           <  2:768917/101‑1 (MQ=12)
             aGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaa                                           >  1:4141491/1‑101 (MQ=21)
              gATCGGCGCGGCGCTGAGGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGCCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          <  2:1431599/101‑1 (MQ=21)
              gATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          <  1:501101/101‑1 (MQ=21)
              gATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          >  1:486256/1‑101 (MQ=21)
               aTCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaaac                                         >  1:1796730/1‑100 (MQ=21)
               aTCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAg                                         <  1:3877528/101‑1 (MQ=21)
               aTCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAg                                         <  1:3485493/101‑1 (MQ=21)
                tCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAgg                                        <  1:1274122/101‑1 (MQ=21)
                tCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAgg                                        <  2:797806/101‑1 (MQ=21)
                 cggcgcggcgCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGCTATTTTGGGCAAGATAAAGGc                                       >  2:1247673/1‑101 (MQ=21)
                   gcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGccc                                     <  2:1544582/101‑1 (MQ=21)
                    cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:4052966/101‑1 (MQ=21)
                    cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    >  2:2537664/1‑101 (MQ=21)
                    cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  2:2557665/101‑1 (MQ=21)
                    cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  2:833553/101‑1 (MQ=21)
                     gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   >  1:4128181/1‑101 (MQ=21)
                     gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   >  1:1049718/1‑101 (MQ=21)
                      cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  >  1:1181649/1‑101 (MQ=21)
                      cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  1:2770012/101‑1 (MQ=21)
                      cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCAGCAAGATAAAGGCCCGGa                                  <  1:3444402/101‑1 (MQ=21)
                       ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 >  2:3714346/1‑101 (MQ=21)
                       ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  1:2541088/101‑1 (MQ=21)
                        gcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAg                                <  1:532589/101‑1 (MQ=21)
                         cgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGc                               >  1:3647200/1‑101 (MQ=21)
                           cTGGAGCGTGTGGCCGACCTGGAAAAAAGGAAGGAGGAGGGCTC..CGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             <  1:3612596/101‑1 (MQ=12)
                           cTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             >  1:203511/1‑101 (MQ=21)
                           cTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             <  1:232449/101‑1 (MQ=21)
                           cTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             >  1:1094809/1‑101 (MQ=21)
                           cTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             >  1:2903827/1‑101 (MQ=21)
                              aaGCGTCTGGGCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          <  2:1784789/101‑1 (MQ=21)
                              aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  1:2649906/1‑101 (MQ=21)
                               aGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  2:3976566/101‑1 (MQ=21)
                               aGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  2:921711/101‑1 (MQ=21)
                                gCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCtt                        <  1:3308880/101‑1 (MQ=21)
                                gCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCtt                        <  1:1367046/101‑1 (MQ=21)
                                gCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCtt                        <  1:1079300/101‑1 (MQ=21)
                                 cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  1:4123114/101‑1 (MQ=21)
                                 cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       >  1:180405/1‑101 (MQ=21)
                                 cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  2:2407748/101‑1 (MQ=21)
                                   tCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTAt                     >  1:656153/1‑101 (MQ=21)
                                   tCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTAt                     <  2:3529722/101‑1 (MQ=21)
                                   tCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTAt                     <  2:777106/101‑1 (MQ=21)
                                    cTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATc                    <  1:23208/101‑1 (MQ=21)
                                     tGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCt                   <  2:3990292/101‑1 (MQ=21)
                                     tGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCt                   >  1:4024707/1‑101 (MQ=21)
                                      ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  2:4180641/101‑1 (MQ=21)
                                      ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  2:928703/101‑1 (MQ=21)
                                      ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  2:2252934/101‑1 (MQ=21)
                                      ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  >  1:2276821/1‑101 (MQ=21)
                                         cGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGcc               >  2:2283685/1‑101 (MQ=21)
                                         cGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGcc               <  1:1576435/101‑1 (MQ=21)
                                          gAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              <  2:317916/101‑1 (MQ=21)
                                           aaCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             >  1:998422/1‑101 (MQ=21)
                                           aaCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             >  2:1283362/1‑101 (MQ=21)
                                           aaCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             <  2:818523/101‑1 (MQ=21)
                                           aaCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             >  2:1331295/1‑101 (MQ=21)
                                            aCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGa            >  1:1783588/1‑101 (MQ=21)
                                            aCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGa            <  2:3588917/101‑1 (MQ=21)
                                             cTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaa           <  1:2539619/101‑1 (MQ=21)
                                              tCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaaa          <  1:1952853/101‑1 (MQ=21)
                                              tCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaaa          <  1:3111348/101‑1 (MQ=21)
                                               cGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         >  2:820012/1‑101 (MQ=21)
                                               cGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         <  1:1574579/101‑1 (MQ=21)
                                               cGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         >  1:299735/1‑101 (MQ=21)
                                                 gATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       <  2:3160338/101‑1 (MQ=21)
                                                 gATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       >  2:3005797/1‑101 (MQ=21)
                                                 gATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       <  1:610336/101‑1 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  2:1268874/101‑1 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  2:3557905/101‑1 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  2:2074130/101‑1 (MQ=21)
                                                   tAAAGGAAGAAGGGGTGCTCT.C.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATcc     <  2:3727721/101‑1 (MQ=25)
                                                   tAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAACATcc     >  2:3396319/1‑101 (MQ=21)
                                                    aaaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    <  1:1490817/101‑1 (MQ=21)
                                                    aaaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    >  1:997909/1‑101 (MQ=21)
                                                    aaaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    >  1:915196/1‑101 (MQ=21)
                                                     aaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGGTATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   >  1:3001598/1‑101 (MQ=21)
                                                     aaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   >  1:431742/1‑101 (MQ=21)
                                                      aGGAAGAAGGGGGGCTCTGC.CCCCCGCTTC.GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCaa  <  2:1221668/101‑1 (MQ=21)
                                                      aGGAAGAAGGGGGGCTCTGC.CCCCCGCTTC.GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCaa  >  1:1100706/1‑101 (MQ=21)
                                                      aGGAAGAAGGGGGGCTCTGC.CCCCCGCTTC.GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCaa  <  2:1361116/101‑1 (MQ=21)
                                                                                |                                                                            
GAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..C.CGCGCTTGTCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530869‑531022

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG