BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	539,743	T→G	100%	Y201D (TAC→GAC)	RCAP_rcc00505 →	type II secretion system protein E

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	539,743	0	T→G	100.0%	854.7	230	Y201D (TAC→GAC)	RCAP_rcc00505	type II secretion system protein E
Reads supporting (aligned to +/- strand): new base (122/107): ref base (0/1): total (122/108)

Only 100 of 238 total aligned reads displayed.


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840


cGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGAc                                                                                                 <  1:3376848/101‑1 (MQ=255)


 ggACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACg                                                                                                >  2:2307790/1‑101 (MQ=255)


  gACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGc                                                                                               <  2:3738979/101‑1 (MQ=255)


   aCGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGcc                                                                                              <  1:1237793/101‑1 (MQ=255)


   aCGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGcc                                                                                              <  2:1181336/101‑1 (MQ=255)


    cGTGACTTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGccc                                                                                             <  1:3139325/101‑1 (MQ=255)


      tGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGt                                                                                           >  1:4171003/1‑101 (MQ=255)


      tGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGt                                                                                           >  2:2717402/1‑101 (MQ=255)


      tGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGt                                                                                           >  1:262425/1‑101 (MQ=255)


        aCCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCt                                                                                         <  1:3058738/101‑1 (MQ=255)


         ccTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTg                                                                                        <  2:641010/101‑1 (MQ=255)


         ccTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTg                                                                                        >  2:1236127/1‑101 (MQ=255)


          cTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCCATGAAAGCAACCCCTATGTCGACGCCCGTCTgg                                                                                       <  2:1089272/101‑1 (MQ=255)


           ttCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGc                                                                                      >  2:2889684/1‑101 (MQ=255)


            tCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGcc                                                                                     <  2:2368141/101‑1 (MQ=255)


            tCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGcc                                                                                     >  2:1737705/1‑101 (MQ=255)


             cAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCg                                                                                    >  1:2914470/1‑101 (MQ=255)


              aaGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGa                                                                                   <  1:1902049/101‑1 (MQ=255)


                ggATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGCCGCCCGTCTGGCCGATg                                                                                 >  2:457575/1‑101 (MQ=255)


                 gATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATgg                                                                                <  2:1557470/101‑1 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTc                                                                             >  1:965512/1‑101 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTc                                                                             >  1:2367961/1‑101 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTc                                                                             >  2:3497214/1‑101 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTc                                                                             <  1:851306/101‑1 (MQ=255)


                      aaaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcgc                                                                           >  1:2817866/1‑101 (MQ=255)


                       aaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcgcg                                                                          >  1:1252142/1‑101 (MQ=255)


                       aaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcgcg                                                                          >  1:1268600/1‑101 (MQ=255)


                       aaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcgcg                                                                          >  2:3047922/1‑101 (MQ=255)


                          cACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGttt                                                                       >  2:2084217/1‑101 (MQ=255)


                          cACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGttt                                                                       <  2:1304583/101‑1 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTc                                                                      >  1:1048129/1‑101 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTc                                                                      <  2:1746452/101‑1 (MQ=255)


                             cTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCaa                                                                    <  1:2073904/101‑1 (MQ=255)


                             cTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCaa                                                                    >  1:3701941/1‑101 (MQ=255)


                               gTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcg                                                                  >  1:1510017/1‑101 (MQ=255)


                               gTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcg                                                                  >  2:1820778/1‑101 (MQ=255)


                                ttGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcgc                                                                 >  2:3963274/1‑101 (MQ=255)


                                 tGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcgcg                                                                >  2:2930500/1‑101 (MQ=255)


                                 tGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcgcg                                                                <  1:2256113/101‑1 (MQ=255)


                                   cGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGAt                                                              >  2:497214/1‑101 (MQ=255)


                                    ggATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATg                                                             <  1:2472237/101‑1 (MQ=255)


                                    ggATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATg                                                             <  1:1815230/101‑1 (MQ=255)


                                      atcatcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATggg                                                           >  1:3607778/1‑100 (MQ=255)


                                      atcatcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGt                                                           >  1:559735/1‑101 (MQ=255)


                                       tcatcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTg                                                          >  1:1734363/1‑101 (MQ=255)


                                           cGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGcccc                                                      >  1:288833/1‑101 (MQ=255)


                                            gACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGccccc                                                     >  1:3136673/1‑101 (MQ=255)


                                            gACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGccccc                                                     >  2:4139689/1‑101 (MQ=255)


                                             aCAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGAGCCCCCg                                                    <  2:3189508/101‑1 (MQ=255)


                                               aaGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGt                                                  >  2:1334484/1‑101 (MQ=255)


                                               aaGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGt                                                  >  2:3182655/1‑101 (MQ=255)


                                                aGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTc                                                 >  1:752096/1‑101 (MQ=255)


                                                     gtgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCggg                                            >  1:625773/1‑100 (MQ=255)


                                                     gtgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCggg                                            >  2:3309221/1‑100 (MQ=255)


                                                     gtgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCggg                                            >  2:786366/1‑100 (MQ=255)


                                                      tgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTg                                           >  1:779876/1‑101 (MQ=255)


                                                       gtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCggatg                                          <  2:251667/101‑4 (MQ=255)


                                                       gtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTgg                                          >  2:3568325/1‑101 (MQ=255)


                                                       gtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTgg                                          >  1:1002688/1‑101 (MQ=255)


                                                           gcgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATgg                                      <  1:3379419/101‑1 (MQ=255)


                                                            cgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgc                                     >  2:3885589/1‑101 (MQ=255)


                                                            cgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgc                                     >  1:839996/1‑101 (MQ=255)


                                                             ggTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgct                                    <  2:2295038/101‑1 (MQ=255)


                                                             ggTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgct                                    >  2:144766/1‑101 (MQ=255)


                                                              gTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctc                                   >  1:1290502/1‑101 (MQ=255)


                                                              gTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctc                                   <  2:2217606/101‑1 (MQ=255)


                                                                ggggCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgc                                 <  1:1950510/101‑1 (MQ=255)


                                                                  ggcggcGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgctc                               >  1:3195141/1‑101 (MQ=255)


                                                                  ggcggcGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgctc                               <  1:1663250/101‑1 (MQ=255)


                                                                  ggcggcGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGCCGCGGTGGATGgctcgctc                               >  2:4134230/1‑101 (MQ=255)


                                                                   gcggcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGctcgctcg                              >  1:2675204/1‑101 (MQ=255)


                                                                    cggcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGt                             <  2:3833223/101‑1 (MQ=255)


                                                                      gcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtct                           <  2:2180857/101‑1 (MQ=255)


                                                                      gcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtct                           <  1:174485/101‑1 (MQ=255)


                                                                        gtgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCg                         <  1:1611314/101‑1 (MQ=255)


                                                                        gtgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCg                         >  2:4164723/1‑101 (MQ=255)


                                                                        gtgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGAGGGGGCCCCCGGGCGCGGGGGGTGGGTCGCTCGTCTCg                         >  1:133952/1‑101 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGAAGGTGCCACCGGTCGCGGTGGATGgctcgctcagccggaccc                     >  2:1497885/1‑91 (MQ=255)


                                                                             gATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCg                    <  2:622322/101‑1 (MQ=255)


                                                                              aTGAAAGCACCCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCgg                   <  2:2634177/101‑1 (MQ=255)


                                                                              aTGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCgg                   >  1:2997697/1‑101 (MQ=255)


                                                                                gAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGaa                 >  2:1992458/1‑101 (MQ=255)


                                                                                   aGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAAtt              <  1:3309462/101‑1 (MQ=255)


                                                                                      aaCCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaa           <  2:2121392/101‑1 (MQ=255)


                                                                                      aaCCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaa           >  1:3986050/1‑101 (MQ=255)


                                                                                      aaCCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaa           <  1:364308/101‑1 (MQ=255)


                                                                                      aaCCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGGGGATGGCTCGCTCGTCTCGATCCGGAAATTcca           >  1:4236191/1‑99 (MQ=255)


                                                                                        ccccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaaga         <  1:3882640/101‑1 (MQ=255)


                                                                                        ccccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaaga         >  1:1117261/1‑101 (MQ=255)


                                                                                        ccccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaaga         <  2:790089/101‑1 (MQ=255)


                                                                                          ccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       <  2:1002597/101‑1 (MQ=255)


                                                                                          ccTAGGTCGGCGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATTGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       <  1:714052/101‑1 (MQ=255)


                                                                                           cTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAgg      >  2:3382973/1‑101 (MQ=255)


                                                                                            tATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGa     <  2:2436359/101‑1 (MQ=255)


                                                                                             aTGTCGCCGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGAc    <  1:3497466/101‑1 (MQ=255)


                                                                                             aTGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGAc    <  1:2114571/101‑1 (MQ=255)


                                                                                             aTGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGAc    >  2:686540/1‑101 (MQ=255)


                                                                                              tGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACa   <  1:2528205/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  2:3695903/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  2:1252046/101‑1 (MQ=255)


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 28 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG