Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 566,409 A→G 100% F80L (TTC→CTC)  RCAP_rcc00528 ← carboxymuconolactone decarboxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034566,4090A→G100.0% 781.3 210F80L (TTC→CTC) RCAP_rcc00528carboxymuconolactone decarboxylase
Reads supporting (aligned to +/- strand):  new base (111/99):  ref base (0/0):  total (111/99)

Only 100 of 217 total aligned reads displayed.

CTCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAATTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAT  >  NC_014034/566311‑566506
                                                                                                  |                                                                                                 
cTCGGCGGCACGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGtt                                                                                                 <  1:1865571/101‑1 (MQ=255)
 tCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTc                                                                                                <  2:794985/101‑1 (MQ=255)
  cggcggCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTcc                                                                                               <  2:3333859/101‑1 (MQ=255)
   ggcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCt                                                                                              <  2:3912155/101‑1 (MQ=255)
   ggcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCt                                                                                              >  1:2652767/1‑101 (MQ=255)
       ggaaGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcg                                                                                          <  1:740587/99‑1 (MQ=255)
       gcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcg                                                                                          <  2:512692/101‑1 (MQ=255)
        cAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCCCGGCGCAGATGTCGGCGAGTGCCTcggca                                                                                         <  2:107041/101‑4 (MQ=255)
          aGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGt                                                                                       <  2:689587/101‑1 (MQ=255)
          aGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGt                                                                                       <  2:213593/101‑1 (MQ=255)
           gTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATCCATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTg                                                                                      <  1:2720433/101‑1 (MQ=255)
           gTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTg                                                                                      >  2:4028496/1‑101 (MQ=255)
            ttCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgc                                                                                     >  2:2918765/1‑101 (MQ=255)
              cGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccg                                                                                   <  1:2190818/101‑1 (MQ=255)
              cGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccg                                                                                   >  2:2947975/1‑101 (MQ=255)
               gTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccgc                                                                                  >  2:1940815/1‑101 (MQ=255)
                tCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccgcc                                                                                 <  2:2733432/101‑1 (MQ=255)
                tCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccgcc                                                                                 <  1:3568709/101‑1 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATGGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                <  1:606544/101‑1 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                <  1:4185147/101‑1 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                >  1:3505476/1‑101 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                <  2:2582115/101‑1 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                >  1:1601451/1‑101 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                <  2:3482487/101‑1 (MQ=255)
                  ccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgcccg                                                                               >  2:927379/1‑101 (MQ=255)
                   cagcagGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgcccgg                                                                              >  2:1209276/1‑100 (MQ=255)
                    agcagGCCAAGGCATGGGCCGCATACCTCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCcgcg                                                                             <  2:704443/101‑1 (MQ=255)
                      cagGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCcgcgcg                                                                           >  2:1290090/1‑101 (MQ=255)
                       agGCCAAGGCATGGGGCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCgcgcgc                                                                          >  1:3564667/1‑101 (MQ=255)
                       agGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCgcgcgc                                                                          <  2:1995303/101‑1 (MQ=255)
                       agGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCgcgcgc                                                                          >  1:3321653/1‑101 (MQ=255)
                         gCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcat                                                                        <  2:3041428/101‑1 (MQ=255)
                          ccAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatc                                                                       >  2:643304/1‑101 (MQ=255)
                            aaGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcaccac                                                                     >  1:3245180/1‑100 (MQ=255)
                             aGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCatcatc                                                                    >  2:2118988/1‑101 (MQ=255)
                             aGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCatcatc                                                                    >  1:2415942/1‑101 (MQ=255)
                               gCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcgc                                                                  >  2:3725776/1‑101 (MQ=255)
                               gCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcgc                                                                  >  1:985499/1‑101 (MQ=255)
                                cATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcgcg                                                                 >  1:4061512/1‑101 (MQ=255)
                                 aTGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCgcgc                                                                >  1:2322096/1‑101 (MQ=255)
                                  tGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGcc                                                               >  2:2408284/1‑101 (MQ=255)
                                   gggCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCg                                                              >  2:34217/1‑101 (MQ=255)
                                     gCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAt                                                            <  1:34232/101‑1 (MQ=255)
                                     gCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAt                                                            <  1:2493649/101‑1 (MQ=255)
                                       cGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgt                                                          <  2:3205473/101‑1 (MQ=255)
                                       cGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgt                                                          <  2:2652561/101‑1 (MQ=255)
                                        gCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgtg                                                         <  2:2783510/101‑1 (MQ=255)
                                        gCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgtg                                                         >  1:1736111/1‑101 (MQ=255)
                                        gCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgtg                                                         >  2:4051655/1‑101 (MQ=255)
                                        gCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgtg                                                         >  1:1034215/1‑101 (MQ=255)
                                        gCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgtg                                                         >  1:2965718/1‑101 (MQ=255)
                                         cATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGa                                                        >  2:4185230/1‑101 (MQ=255)
                                          aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       >  2:3339723/1‑101 (MQ=255)
                                           tACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGGGAGCTCCTCCCGCGTGCCGCCCGCGCGCATCATCGCGcccgtggcaaa                                                      >  2:443914/1‑92 (MQ=255)
                                           tACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaaa                                                      >  1:1274934/1‑101 (MQ=255)
                                           tACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaaa                                                      >  2:4134036/1‑101 (MQ=255)
                                             catcatCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGc                                                    <  1:3874967/101‑1 (MQ=255)
                                              atcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGcc                                                   >  2:2970745/1‑101 (MQ=255)
                                              atcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGcc                                                   <  2:1735064/101‑1 (MQ=255)
                                               tcatcGCCGGGCCGCCGCCCATCTGGATCGCCGCGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCg                                                  >  2:3880627/1‑101 (MQ=255)
                                               tcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCg                                                  <  1:1431842/101‑1 (MQ=255)
                                               tcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCg                                                  <  1:2788772/101‑1 (MQ=255)
                                               tcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCg                                                  >  2:3259951/1‑101 (MQ=255)
                                                 atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGAt                                                >  1:3679222/1‑101 (MQ=255)
                                                 atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGAt                                                <  1:514924/101‑1 (MQ=255)
                                                  tcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATg                                               <  1:2585876/101‑1 (MQ=255)
                                                    gCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCa                                             >  2:2210281/1‑101 (MQ=255)
                                                     ccGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGGGCATCATCGCGCCGATGTGAAAGCCGATGCAg                                            >  1:1536277/1‑101 (MQ=255)
                                                      cGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAgg                                           <  1:1364886/101‑1 (MQ=255)
                                                        ggCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGc                                         >  2:1421907/1‑101 (MQ=255)
                                                              cgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAg                                   >  1:1101920/1‑101 (MQ=255)
                                                                cccATCTGGATCGCCACGGCGCAGATGTCGGAGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgcg                                 >  2:1864852/1‑101 (MQ=255)
                                                                  cATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCt                               >  2:2087429/1‑101 (MQ=255)
                                                                   aTCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTg                              >  1:2078455/1‑101 (MQ=255)
                                                                     cTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTgcg                            >  1:2113463/1‑101 (MQ=255)
                                                                      tGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTgcgc                           >  1:160720/1‑101 (MQ=255)
                                                                        gATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGa                         >  1:754654/1‑101 (MQ=255)
                                                                          tCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATg                       >  1:1926437/1‑101 (MQ=255)
                                                                          tCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATg                       <  2:1500020/101‑1 (MQ=255)
                                                                           cGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGc                      <  2:84766/101‑1 (MQ=255)
                                                                           cGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGCGCTCGCAGCGCTGCGCGATGc                      <  1:3121001/101‑1 (MQ=255)
                                                                            gCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGcc                     >  2:1624357/1‑101 (MQ=255)
                                                                             ccACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGccc                    <  1:1209370/101‑1 (MQ=255)
                                                                              cACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCa                   >  1:3070200/1‑101 (MQ=255)
                                                                               ccGGCGCAGATGTCGGCGAGTTCCTCGCGCGCGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCAt                  <  1:559504/100‑1 (MQ=255)
                                                                                cGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATg                 <  2:1773145/101‑1 (MQ=255)
                                                                                 ggCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATgg                <  1:3532939/101‑1 (MQ=255)
                                                                                 ggCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATgg                >  1:2672835/1‑101 (MQ=255)
                                                                                 ggCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATgg                >  2:189215/1‑101 (MQ=255)
                                                                                    gcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCa             <  1:2030531/101‑1 (MQ=255)
                                                                                    gcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCa             <  2:1222933/101‑1 (MQ=255)
                                                                                    gcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCa             >  2:2242246/1‑101 (MQ=255)
                                                                                     cAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATTGCGCCAATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGcctg            >  2:1530330/1‑99 (MQ=255)
                                                                                     cAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAg            <  2:580677/101‑1 (MQ=255)
                                                                                     cAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGAGGCCCATGGCCAg            <  1:2288240/101‑1 (MQ=255)
                                                                                       gATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcg          <  1:3998324/101‑1 (MQ=255)
                                                                                       gATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcg          >  1:2452050/1‑101 (MQ=255)
                                                                                        aTGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcgc         >  1:896265/1‑101 (MQ=255)
                                                                                             ggCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATcc    <  2:3669429/101‑1 (MQ=255)
                                                                                              gCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCa   >  1:441225/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAATTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAT  >  NC_014034/566311‑566506

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG