Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 625,791 G→C 100% R533R (CGG→CGC anfA → nitrogen fixation regulatory protein AnfA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034625,7910G→C100.0% 626.6 168R533R (CGG→CGCanfAnitrogen fixation regulatory protein AnfA
Reads supporting (aligned to +/- strand):  new base (85/83):  ref base (0/0):  total (85/83)

Only 100 of 189 total aligned reads displayed.

ACGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAC  >  NC_014034/625693‑625888
                                                                                                  |                                                                                                 
acgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTa                                                                                                 <  2:2395269/101‑1 (MQ=255)
acgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTa                                                                                                 >  2:3792174/1‑101 (MQ=255)
acgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTa                                                                                                 >  1:1402653/1‑101 (MQ=255)
 cgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGACGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTAt                                                                                                <  1:1999151/101‑1 (MQ=255)
     ccAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcgcg                                                                                            <  1:3172857/101‑1 (MQ=255)
      cAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCgcgc                                                                                           >  1:161673/1‑101 (MQ=255)
       aGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCt                                                                                          >  1:1530567/1‑101 (MQ=255)
          gCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCg                                                                                       <  2:1293289/101‑1 (MQ=255)
          gCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCg                                                                                       <  2:573202/101‑1 (MQ=255)
          gCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCg                                                                                       <  1:1042384/101‑1 (MQ=255)
            aaTATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCCCTATCCCGCTTCGGc                                                                                     >  1:1856599/1‑101 (MQ=255)
            aaTATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCCGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGc                                                                                     >  1:2364162/1‑101 (MQ=255)
             atatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGcc                                                                                    <  2:2142069/101‑1 (MQ=255)
             atatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGcc                                                                                    >  1:4218374/1‑101 (MQ=255)
             atatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGcc                                                                                    >  1:2778628/1‑101 (MQ=255)
             atatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGcc                                                                                    >  2:3588637/1‑101 (MQ=255)
              tatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCt                                                                                   <  1:360106/101‑1 (MQ=255)
               atCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTg                                                                                  >  1:1334509/1‑101 (MQ=255)
               atCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTg                                                                                  >  1:1157517/1‑101 (MQ=255)
               atCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCCCCCTTCGGCCTg                                                                                  >  1:3321979/1‑101 (MQ=255)
                 cGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGAt                                                                                >  2:2461949/1‑101 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                               >  1:2426929/1‑101 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                               <  1:2976408/101‑1 (MQ=255)
                   gCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGAATCGACACGCACCGCTATAGCGCTTCGGCCTGAttg                                                                              >  2:4036477/1‑99 (MQ=255)
                    ccAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCgc                                                                             >  2:779679/1‑100 (MQ=255)
                     cAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGAc                                                                            >  2:2864901/1‑101 (MQ=255)
                      gggcggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                           <  2:1956436/100‑1 (MQ=255)
                      aGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                           >  1:3049443/1‑101 (MQ=255)
                      aGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                           <  1:3339597/101‑1 (MQ=255)
                      aGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                           >  2:2810619/1‑101 (MQ=255)
                      aGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                           >  2:3143635/1‑101 (MQ=255)
                      aGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                           >  2:470399/1‑101 (MQ=255)
                       ggcggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACgg                                                                          <  2:2386868/101‑1 (MQ=255)
                       ggcggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACgg                                                                          >  1:1451133/1‑101 (MQ=255)
                         cggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGcc                                                                        >  1:341742/1‑101 (MQ=255)
                          ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                       >  1:831517/1‑101 (MQ=255)
                             cGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTCCGGCCTGATCGACGGCCAATg                                                                    <  1:2406827/101‑1 (MQ=255)
                              gAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATgc                                                                   >  2:1420005/1‑100 (MQ=255)
                              gAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGa                                                                   >  1:2881502/1‑101 (MQ=255)
                                 cTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCg                                                                >  2:3453594/1‑101 (MQ=255)
                                  ttttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGa                                                               >  2:4194167/1‑101 (MQ=255)
                                    ttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAAt                                                             >  1:2161375/1‑101 (MQ=255)
                                    ttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAAt                                                             <  1:3792431/101‑1 (MQ=255)
                                     tGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATa                                                            >  1:4076100/1‑101 (MQ=255)
                                     tGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATa                                                            <  1:150929/101‑1 (MQ=255)
                                      gCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAc                                                           >  2:3422159/1‑101 (MQ=255)
                                      gCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAc                                                           <  1:1629528/101‑1 (MQ=255)
                                        aGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCg                                                         <  2:664208/101‑1 (MQ=255)
                                         ggTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCgg                                                        <  1:4156917/101‑1 (MQ=255)
                                          gTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGTATCGACCCGCCCCGCCATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGc                                                       >  2:2664413/1‑101 (MQ=255)
                                           tctcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGAACCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCTGcc                                                      >  2:1796478/1‑101 (MQ=255)
                                            ctcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGccc                                                     <  2:967600/101‑1 (MQ=255)
                                              cgcgCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGCCCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCg                                                   >  2:1796379/1‑101 (MQ=255)
                                              cgcgCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCg                                                   <  1:193107/101‑1 (MQ=255)
                                               gcgcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGc                                                  <  1:2762449/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                               <  2:4104745/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                               <  1:1808787/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                               <  2:3347926/101‑1 (MQ=255)
                                                   cGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcg                                              <  2:1755523/101‑1 (MQ=255)
                                                    gggTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGTGCTTCGGCCTGATCGACGGCCAATGACCGAATACCCGCCCCGCTTcgc                                             >  1:3352544/1‑100 (MQ=255)
                                                    gggTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcgg                                             <  2:1778525/101‑1 (MQ=255)
                                                    gggTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcgg                                             >  1:972280/1‑101 (MQ=255)
                                                    gggTGCTGGGCCTGCGGATGCGGCGGCTCGGCACTCACCCGCACCGCTATTGCGCGTCGGCCCGATGGACGGCCAAGGACCGAATACCGGCCCTGCTTcgg                                             >  2:2781119/1‑101 (MQ=255)
                                                      gTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggcg                                           <  2:3855057/101‑1 (MQ=255)
                                                       tGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggcgg                                          <  2:2191854/101‑1 (MQ=255)
                                                       tGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggcgg                                          <  2:4063024/101‑1 (MQ=255)
                                                        gCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCggg                                         >  1:1487170/1‑101 (MQ=255)
                                                        gCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCggg                                         >  2:1476777/1‑101 (MQ=255)
                                                            ggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCtt                                     <  2:371951/101‑1 (MQ=255)
                                                            ggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCtt                                     <  2:40881/101‑1 (MQ=255)
                                                               cTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCttttt                                  >  2:2518636/1‑101 (MQ=255)
                                                               cTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCttttt                                  >  2:4136333/1‑101 (MQ=255)
                                                                 gCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCGTTTTCt                                >  2:846377/1‑101 (MQ=255)
                                                                  cGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                               <  2:3321738/101‑1 (MQ=255)
                                                                   ggATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCttt                              >  2:3267880/1‑101 (MQ=255)
                                                                     aTGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGc                            >  1:1293268/1‑101 (MQ=255)
                                                                      tGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCg                           <  2:3941238/101‑1 (MQ=255)
                                                                        gggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGc                         <  2:1619691/101‑1 (MQ=255)
                                                                        gggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGc                         <  2:198457/101‑1 (MQ=255)
                                                                         ggcggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCa                        >  1:3393601/1‑101 (MQ=255)
                                                                           cggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAgg                      >  2:3285074/1‑100 (MQ=255)
                                                                            ggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAggc                     >  2:2262534/1‑99 (MQ=255)
                                                                            ggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGcc                     >  2:3234196/1‑101 (MQ=255)
                                                                             gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGccc                    <  2:1108586/101‑1 (MQ=255)
                                                                                cGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGtt                 <  2:1368116/101‑1 (MQ=255)
                                                                                 ggCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGGCCGGTc                >  2:2693361/1‑101 (MQ=255)
                                                                                 ggCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTc                <  1:2536280/101‑1 (MQ=255)
                                                                                 ggCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGGTc                >  2:3674853/1‑101 (MQ=255)
                                                                                    aTCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGgcccctccgg             >  1:3673202/1‑94 (MQ=255)
                                                                                     tCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGaa            <  2:3902340/101‑1 (MQ=255)
                                                                                       gACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGGCCCTTCGGAAtt          >  2:311426/1‑101 (MQ=255)
                                                                                         cccGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTcc        <  2:1293172/101‑1 (MQ=255)
                                                                                           cGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGt      >  2:2305308/1‑101 (MQ=255)
                                                                                            gCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGGCCGTTCCGAACTcccct     >  1:684445/1‑98 (MQ=255)
                                                                                             gaCCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGttt    <  1:3890116/100‑1 (MQ=255)
                                                                                             ctccGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGttt    <  2:156989/99‑1 (MQ=255)
                                                                                             cACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGGCCGGTCGGGATTTCGttt    >  2:2243370/1‑101 (MQ=255)
                                                                                             cACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGGCCCTTCGGGATTCCGttt    >  1:524061/1‑101 (MQ=255)
                                                                                              aCCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGttt  >  1:1742053/1‑100 (MQ=255)
                                                                                               ccGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAc  <  1:816842/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
ACGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAC  >  NC_014034/625693‑625888

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG