BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	66,387	T→G	100%	S518S (TCT→TCG)	flhA →	flagellar biosynthesis protein FlhA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	66,387	0	T→G	100.0%	749.3	201	S518S (TCT→TCG)	flhA	flagellar biosynthesis protein FlhA
Reads supporting (aligned to +/- strand): new base (114/87): ref base (0/0): total (114/87)

Only 100 of 208 total aligned reads displayed.


GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484


gTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGaa                                                                                                 >  2:1110122/1‑101 (MQ=255)


  gATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAcc                                                                                               <  1:4111049/101‑1 (MQ=255)


   aTCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccg                                                                                              <  1:1035738/101‑1 (MQ=255)


     ccGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccgcc                                                                                            <  1:420168/101‑1 (MQ=255)


      cGGCGCACCTTCGGCCGTGTGATGACGCTGGGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccgccg                                                                                           <  2:715309/101‑1 (MQ=255)


       ggCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACcgccgc                                                                                          <  2:3081238/101‑1 (MQ=255)


          gcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTg                                                                                       >  1:3074603/1‑101 (MQ=255)


           cAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGc                                                                                      >  2:3023335/1‑101 (MQ=255)


            agcTTCGGCCTTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCt                                                                                     <  1:1662236/99‑1 (MQ=255)


            aaCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCt                                                                                     <  1:3423348/101‑1 (MQ=255)


             aCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCtt                                                                                    >  1:2849920/1‑101 (MQ=255)


             aCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCtt                                                                                    <  2:179554/101‑1 (MQ=255)


              cTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTg                                                                                   >  2:2608130/1‑101 (MQ=255)


              cTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTg                                                                                   >  1:3948842/1‑101 (MQ=255)


              cTTCGGCCGGCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTg                                                                                   <  1:3337659/101‑1 (MQ=255)


               ttCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGa                                                                                  <  1:2922159/101‑1 (MQ=255)


                 cGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACg                                                                                >  2:182604/1‑101 (MQ=255)


                 cGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACg                                                                                >  2:3794518/1‑101 (MQ=255)


                  ggCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGa                                                                               <  2:3249353/101‑1 (MQ=255)


                      gTCTGATGACGCTGCGGGCGCGGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTg                                                                           <  1:295740/101‑1 (MQ=255)


                       tCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGa                                                                          >  1:524041/1‑101 (MQ=255)


                        cTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGAt                                                                         >  2:2728112/1‑101 (MQ=255)


                          gatgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcc                                                                       >  1:1239844/1‑101 (MQ=255)


                           atgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATccc                                                                      <  1:3162273/101‑1 (MQ=255)


                             gaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    >  2:1280000/1‑101 (MQ=255)


                             gaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    <  2:528305/101‑1 (MQ=255)


                              ccGCTGCGGGCCCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGa                                                                   <  1:3696768/100‑1 (MQ=255)


                               cGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGAc                                                                  <  1:740072/101‑1 (MQ=255)


                                gCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGaaa                                                                 >  1:2514209/1‑99 (MQ=255)


                                 cTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACaa                                                                <  2:890535/101‑1 (MQ=255)


                                 cTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACaa                                                                <  1:2608321/101‑1 (MQ=255)


                                  tGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAg                                                               <  1:1503312/101‑1 (MQ=255)


                                   gCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAgg                                                              <  1:2009084/101‑1 (MQ=255)


                                    cGGGCGGTGCGCCGCCGGGTGGGGGAAGTGTTCAACCTTTCCGATCCCGCCCGCGCCGAGTCAAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGt                                                             <  1:949746/101‑1 (MQ=255)


                                    cGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGt                                                             <  2:1801377/101‑1 (MQ=255)


                                     gggCGCTGCGCCGCCTGCTCGAGGAAGTGTGGAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGtt                                                            >  1:3747302/1‑101 (MQ=255)


                                     gggCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGtt                                                            >  2:38979/1‑101 (MQ=255)


                                     gggCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGtt                                                            <  2:2899700/101‑1 (MQ=255)


                                      ggCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTc                                                           >  1:1485265/1‑101 (MQ=255)


                                       gcgcTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTcc                                                          <  1:325260/101‑1 (MQ=255)


                                        cgcTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCGGATCCCCGACAAGGTTCCg                                                         >  1:1102753/1‑101 (MQ=255)


                                          cTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGt                                                       >  2:3619199/1‑101 (MQ=255)


                                          cTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGt                                                       <  1:2641359/101‑1 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      >  1:2203998/1‑101 (MQ=255)


                                            gcgcCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCg                                                     <  1:1952910/101‑1 (MQ=255)


                                               ccgccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATc                                                  >  1:3353787/1‑101 (MQ=255)


                                                cgccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATct                                                 <  2:3648616/101‑1 (MQ=255)


                                                 gccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctg                                                >  2:2323096/1‑101 (MQ=255)


                                                   cTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgct                                              >  1:3488896/1‑101 (MQ=255)


                                                   cTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgct                                              >  1:2238612/1‑101 (MQ=255)


                                                   cTGCTCGAGGAAGGTTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgct                                              <  1:3721247/101‑1 (MQ=255)


                                                    tGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctg                                             <  2:1358752/101‑1 (MQ=255)


                                                    tGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctg                                             >  2:1720645/1‑101 (MQ=255)


                                                       tCGAGGAAGTGTGCTACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgctg                                          <  1:3649818/101‑1 (MQ=255)


                                                       tCGAGGAAGTGTGCACCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgctg                                          <  1:311122/101‑1 (MQ=255)


                                                         gAGGAAGTGTGCACCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        <  1:126023/101‑1 (MQ=255)


                                                         gAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        >  2:2457992/1‑101 (MQ=255)


                                                         gAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        >  1:1633938/1‑101 (MQ=255)


                                                          gggAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCg                                       <  2:1005905/100‑1 (MQ=255)


                                                          aGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCg                                       <  1:3870074/101‑1 (MQ=255)


                                                          aGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCg                                       >  2:1516393/1‑101 (MQ=255)


                                                           ggAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCgg                                      >  2:3512620/1‑101 (MQ=255)


                                                            gaagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGt                                     <  2:3632576/98‑1 (MQ=255)


                                                             aagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtg                                    >  1:3849331/3‑101 (MQ=255)


                                                             aagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtg                                    >  2:3816607/3‑101 (MQ=255)


                                                               gtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgct                                  <  1:4089613/101‑1 (MQ=255)


                                                               gtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgct                                  >  1:597177/1‑101 (MQ=255)


                                                                ggtgCACCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgctg                                 <  2:3436541/100‑1 (MQ=255)


                                                                  tgCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCg                               >  2:198087/1‑101 (MQ=255)


                                                                  tgCAACCTTTCCGACCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCg                               <  2:1647188/101‑1 (MQ=255)


                                                                   gCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGt                              >  2:1989146/1‑101 (MQ=255)


                                                                    cacccTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTc                             <  2:3984618/98‑1 (MQ=255)


                                                                      cccTTCCCGACCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctg                           <  2:772646/100‑1 (MQ=255)


                                                                      aCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctg                           <  1:90146/101‑1 (MQ=255)


                                                                       ccTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgc                          <  2:1485163/101‑1 (MQ=255)


                                                                       ccTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgc                          >  2:2580031/1‑101 (MQ=255)


                                                                       ccTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGGGCTGCGTctgc                          >  2:1946973/1‑101 (MQ=255)


                                                                         tttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctg                        <  1:2834800/101‑1 (MQ=255)


                                                                         tttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGGctgctg                        >  2:3738899/1‑101 (MQ=255)


                                                                           tCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgct                      >  1:140392/1‑101 (MQ=255)


                                                                            ccGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgctg                     >  2:439619/1‑101 (MQ=255)


                                                                             tgttcaggtccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTgg                    >  2:517873/10‑101 (MQ=255)


                                                                              gATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGga                   >  1:786737/1‑101 (MQ=255)


                                                                               accccgcccgCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaa                  <  2:1216617/99‑1 (MQ=255)


                                                                                tCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaag                 <  2:1698837/101‑1 (MQ=255)


                                                                                ccccgcccgCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaag                 <  1:453268/100‑1 (MQ=255)


                                                                                 cccgcccgCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaaga                <  2:3148721/101‑1 (MQ=255)


                                                                                   cgcccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAAc              <  2:840715/101‑1 (MQ=255)


                                                                                     cccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACgg            <  2:3488644/101‑1 (MQ=255)


                                                                                     cccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACgg            >  1:1777043/1‑101 (MQ=255)


                                                                                     cccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACgg            <  2:2111736/101‑1 (MQ=255)


                                                                                        gcggCGAGTCGAACCGCCGCCTGCTTGACGAGCTGAGCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGGCTGCTGCTGGAAGAACGGgtg         >  2:386500/1‑101 (MQ=255)


                                                                                        gcgcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgtg         <  1:566553/101‑1 (MQ=255)


                                                                                        gcgcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgtg         >  2:4125746/1‑101 (MQ=255)


                                                                                          gcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGGGTc       >  2:668331/1‑101 (MQ=255)


                                                                                            cGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGa     >  1:2449508/1‑101 (MQ=255)


                                                                                            cGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGa     >  1:2394128/1‑101 (MQ=255)


                                                                                             gAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGAt    >  1:756216/1‑101 (MQ=255)


                                                                                              aGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATc   >  1:2566619/1‑101 (MQ=255)


                                                                                               gTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATcc  >  2:1912885/1‑101 (MQ=255)


GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG