Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,564 G→A 2.5% S1681S (AGC→AGT)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,5640G→A 2.5% 2.9 0.0 ‑0.3160S1681S (AGC→AGT) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (79/77):  total (81/79)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.08e-01

Only 100 of 175 total aligned reads displayed.

CCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGG  >  NC_014034/678464‑678664
                                                                                                    |                                                                                                    
ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                                                                      <  1:303608/101‑1 (MQ=255)
 cgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCgcgc                                                                                                     >  1:2256692/1‑101 (MQ=255)
  gccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCt                                                                                                    >  2:3170382/1‑101 (MQ=255)
   ccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTc                                                                                                   <  1:265326/101‑1 (MQ=255)
     gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                                                                 >  2:27946/1‑101 (MQ=255)
       ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                                                               >  2:3200483/1‑101 (MQ=255)
       ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGCTGACCGCCGCGCTCGACa                                                                                               >  2:1659322/1‑101 (MQ=255)
        gTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCTCCGTGCCAAGGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAg                                                                                              <  1:575021/101‑1 (MQ=255)
         tCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAgc                                                                                             >  1:1991933/1‑101 (MQ=255)
           aGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAgcgc                                                                                           >  1:3002062/1‑101 (MQ=255)
            gcgcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgcc                                                                                          >  1:3887888/1‑101 (MQ=255)
             cgcGGGAATGACCGCGCTGCCCAGCGCCGCGATTTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                                                                         <  1:3602234/101‑1 (MQ=255)
              gcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccgc                                                                                        <  1:1102893/101‑1 (MQ=255)
               ccGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCcgcg                                                                                       <  1:3585777/101‑1 (MQ=255)
               ccGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCcgcg                                                                                       >  2:3424816/1‑101 (MQ=255)
                cGGAATGACCGCGCCGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGa                                                                                      >  2:1365768/1‑101 (MQ=255)
                   aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                                                                   <  2:658935/101‑1 (MQ=255)
                    aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                                                                  <  1:3290627/101‑1 (MQ=255)
                       aCCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcgc                                                                               >  1:1263303/1‑101 (MQ=255)
                        ccGCGCTGCCCAGCGCCGCGATCTCCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGcc                                                                              <  2:2624946/101‑1 (MQ=255)
                         cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                             >  1:397335/1‑101 (MQ=255)
                         cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                             <  1:3734388/101‑1 (MQ=255)
                         cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                             >  2:516496/1‑101 (MQ=255)
                           cgcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTg                                                                           >  1:2820181/1‑101 (MQ=255)
                             cTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCt                                                                         <  2:480917/101‑1 (MQ=255)
                              tGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGGGCTc                                                                        >  2:1120618/1‑101 (MQ=255)
                               gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                       <  1:3534065/101‑1 (MQ=255)
                               gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                       <  1:1986399/101‑1 (MQ=255)
                                cccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAg                                                                      <  1:3071351/101‑1 (MQ=255)
                                 ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTTCGCCGTGCTCAgc                                                                     >  2:2174497/1‑101 (MQ=255)
                                 ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                     >  2:787833/1‑101 (MQ=255)
                                 ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                     <  1:1689131/101‑1 (MQ=255)
                                 ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                     <  1:1549137/101‑1 (MQ=255)
                                 ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                     <  2:4008200/101‑1 (MQ=255)
                                  cAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGACGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcg                                                                    >  2:3657952/1‑101 (MQ=255)
                                   aGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACGGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcgc                                                                   <  1:2283384/101‑1 (MQ=255)
                                    gcgcCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGTGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgcc                                                                  <  1:3624158/101‑1 (MQ=255)
                                     cgccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccg                                                                 >  1:2576188/1‑101 (MQ=255)
                                      gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                                                                >  2:2682631/1‑101 (MQ=255)
                                        cgctATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGa                                                              <  2:4165335/101‑1 (MQ=255)
                                        cgcgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGa                                                              <  2:3114537/101‑1 (MQ=255)
                                        cgcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                              >  2:2144532/1‑93 (MQ=2)
                                         gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                                                             >  1:72198/1‑92 (MQ=2)
                                          cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                            >  2:3328231/1‑101 (MQ=255)
                                          cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                            >  2:2236756/1‑101 (MQ=255)
                                           gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGCGCTCAGCGCCGCGACtt                                                           >  2:3802485/1‑101 (MQ=255)
                                            aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                          <  2:3023117/101‑1 (MQ=255)
                                            aTCTGCCCCGGGCCAAAGGCCGCCAGCTGCGCGGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                          <  1:1762744/101‑1 (MQ=255)
                                             tCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGc                                                         >  2:2554255/1‑101 (MQ=255)
                                               tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                       >  2:2271508/1‑101 (MQ=255)
                                               tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                       <  2:3001837/101‑1 (MQ=255)
                                               ggcgcCGGGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                       <  1:1350871/100‑1 (MQ=255)
                                                gcgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCATCGCCGCGACTTGCcgc                                                      >  2:2385093/1‑101 (MQ=255)
                                                gcgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgc                                                      <  1:3425061/101‑1 (MQ=255)
                                                  gcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgt                                                    >  1:435824/1‑101 (MQ=255)
                                                      ggCCAACGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGCCAGCGCGGCGAGCGGCGCCGTGCTCAGCGCCGCGGCTTGCCGCgtcgtc                                                <  2:397284/100‑1 (MQ=37)
                                                       gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa                                               >  1:4222665/1‑101 (MQ=255)
                                                        ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                              <  1:55053/101‑1 (MQ=255)
                                                        ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCAGCGTCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                              >  1:3620357/1‑101 (MQ=255)
                                                         cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGc                                             >  2:1435199/1‑101 (MQ=255)
                                                          aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc                                            >  1:3130560/1‑101 (MQ=255)
                                                           aaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc                                           <  1:3200722/101‑1 (MQ=255)
                                                            aGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                                          >  2:827949/1‑101 (MQ=255)
                                                            aGGCCGCCAGCTGCGCCGAGACGAGCGCGGTGACCGCCGCGCTCGAAAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                                          <  1:1959498/101‑1 (MQ=255)
                                                             ggCCGCGAGCCGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAATTGCCGCGTCGTCAGCCCCg                                         <  2:1991807/101‑1 (MQ=255)
                                                             ggCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCg                                         <  2:1244031/101‑1 (MQ=255)
                                                             ggCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCg                                         <  1:310574/101‑1 (MQ=255)
                                                             ggCCGCCAGCGGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCg                                         <  2:1986595/101‑1 (MQ=255)
                                                                   cAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcg                                   <  1:2937292/101‑1 (MQ=255)
                                                                    aGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgc                                  >  2:2490774/1‑101 (MQ=255)
                                                                       tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccgc                               >  2:1950936/1‑101 (MQ=255)
                                                                       tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccgc                               <  2:3881475/101‑1 (MQ=255)
                                                                        gcgcCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCcgcg                              >  2:980818/1‑101 (MQ=255)
                                                                        gcgcCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCcgcg                              <  2:932003/101‑1 (MQ=255)
                                                                          gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAGTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCt                            >  1:3651573/1‑101 (MQ=255)
                                                                          gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCt                            <  1:4008623/101‑1 (MQ=255)
                                                                            cgagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTgg                          >  2:1689342/1‑101 (MQ=255)
                                                                            cgagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTgg                          >  1:1520724/1‑101 (MQ=255)
                                                                               gccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCa                       <  2:2123320/101‑1 (MQ=255)
                                                                               gccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCa                       >  2:178510/1‑101 (MQ=255)
                                                                               gccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCa                       <  1:3156890/101‑1 (MQ=255)
                                                                                 cgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGGGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGc                     >  1:3502953/1‑101 (MQ=255)
                                                                                  gagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGcc                    >  2:2347774/1‑101 (MQ=255)
                                                                                   agcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcg                   <  2:3998505/101‑1 (MQ=255)
                                                                                    gcgcGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgc                  >  1:2973627/1‑101 (MQ=255)
                                                                                     cgcgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcg                 >  2:1281538/1‑101 (MQ=255)
                                                                                     cgcgGTGACCGCCGCGCGCGACAGCGCCGCGACCTGCGCCGTGCCCAGCGCCGCGCCTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcg                 <  1:2766170/101‑1 (MQ=255)
                                                                                      gcgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgc                >  1:3822288/1‑101 (MQ=255)
                                                                                      gcgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgc                >  2:3011998/1‑101 (MQ=255)
                                                                                       cgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgcg               <  1:2627346/101‑1 (MQ=255)
                                                                                          tGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATc            <  1:650684/101‑1 (MQ=255)
                                                                                          tGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATc            <  2:435767/101‑1 (MQ=255)
                                                                                           gACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCt           >  2:1022986/1‑101 (MQ=255)
                                                                                           gACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCt           <  1:2236915/101‑1 (MQ=255)
                                                                                            gccgccgCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTg          <  2:2847426/100‑1 (MQ=255)
                                                                                             ccgccgCGCTCGACAGCGCCGCGAACTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTgc         >  2:2390702/1‑101 (MQ=255)
                                                                                                 cgcgCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCg     >  1:2515670/1‑101 (MQ=255)
                                                                                                   cgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTg   >  1:4192706/1‑101 (MQ=255)
                                                                                                    gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg  <  2:621904/101‑1 (MQ=255)
                                                                                                    gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg  <  1:1075262/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGG  >  NC_014034/678464‑678664

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG