Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,586 G→T 2.6% A1674E (GCG→GAG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,5860G→T 2.6% 2.8 0.0 ‑0.6153A1674E (GCG→GAG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (72/77):  total (74/79)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.48e-01

Only 100 of 162 total aligned reads displayed.

GACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGGCGCGGATCTGCGCCGT  >  NC_014034/678486‑678686
                                                                                                    |                                                                                                    
gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcg                                                                                                      >  2:1307727/1‑101 (MQ=255)
  ccGCGCTGCCCAGCGCCGCGATCTCCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGcc                                                                                                    <  2:2624946/101‑1 (MQ=255)
   cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                                                   >  1:397335/1‑101 (MQ=255)
   cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                                                   >  2:516496/1‑101 (MQ=255)
   cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                                                   >  1:2251197/1‑101 (MQ=255)
     cgcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTg                                                                                                 >  1:2820181/1‑101 (MQ=255)
      gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                                                                                                >  1:1986721/1‑101 (MQ=255)
       cTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCt                                                                                               <  2:3614082/101‑1 (MQ=255)
        tGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGGGCTc                                                                                              >  2:1120618/1‑101 (MQ=255)
         gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                                             <  1:3534065/101‑1 (MQ=255)
         gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                                             >  2:475256/1‑101 (MQ=255)
         gCCCACCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                                             <  1:1307823/101‑1 (MQ=255)
          gcaaccgccgcGATCTGCGCCGCGCTCAGCGCGTCGAGCTGCGCGGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAg                                                                                            <  1:3621716/96‑1 (MQ=2)
          cccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAg                                                                                            <  2:3824975/101‑1 (MQ=255)
          cccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAg                                                                                            <  1:3071351/101‑1 (MQ=255)
           ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTTCGCCGTGCTCAgc                                                                                           >  2:2174497/1‑101 (MQ=255)
           ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                                           <  1:1549137/101‑1 (MQ=255)
           ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                                           >  2:787833/1‑101 (MQ=255)
           ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                                           <  2:4008200/101‑1 (MQ=255)
            cAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcg                                                                                          >  2:2527709/1‑101 (MQ=255)
             aGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACGGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcgc                                                                                         <  1:2283384/101‑1 (MQ=255)
               cgccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccg                                                                                       >  1:2576188/1‑101 (MQ=255)
               cgccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccg                                                                                       >  1:1636551/1‑101 (MQ=255)
                gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                                                                                      <  2:1806672/101‑1 (MQ=255)
                gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                                                                                      >  2:2682631/1‑101 (MQ=255)
                    cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                                                  >  2:3328231/1‑101 (MQ=255)
                     gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGCGCTCAGCGCCGCGACtt                                                                                 >  2:3802485/1‑101 (MQ=255)
                      tgcTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                                                >  2:980254/3‑101 (MQ=255)
                      aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGNGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                                                >  1:969489/1‑101 (MQ=255)
                      aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                                                <  2:3023117/101‑1 (MQ=255)
                       tCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGc                                                                               >  2:3884798/1‑101 (MQ=255)
                         tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                                             <  2:3001837/101‑1 (MQ=255)
                          gcgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCATCGCCGCGACTTGCcgc                                                                            >  2:2385093/1‑101 (MQ=255)
                          gcgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgc                                                                            <  1:3425061/101‑1 (MQ=255)
                            gcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgt                                                                          >  1:435824/1‑101 (MQ=255)
                              cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg                                                                        >  2:3341533/1‑101 (MQ=255)
                              cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg                                                                        >  1:234227/1‑101 (MQ=255)
                              cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg                                                                        >  2:3156651/1‑101 (MQ=255)
                                ggCCAACGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGCCAGCGCGGCGAGCGGCGCCGTGCTCAGCGCCGCGGCTTGCCGCgtcgtc                                                                      <  2:397284/100‑1 (MQ=37)
                                 gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa                                                                     <  1:301845/101‑1 (MQ=255)
                                 gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa                                                                     >  1:4222665/1‑101 (MQ=255)
                                  ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                                                    <  1:55053/101‑1 (MQ=255)
                                  ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCAGCGTCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                                                    >  1:3620357/1‑101 (MQ=255)
                                   cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGc                                                                   >  2:1435199/1‑101 (MQ=255)
                                      aGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                                                                >  2:827949/1‑101 (MQ=255)
                                      aGGCCGCCAGCTGCGCCGAGACGAGCGCGGTGACCGCCGCGCTCGAAAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                                                                <  1:1959498/101‑1 (MQ=255)
                                       ggCCGCGAGCCGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAATTGCCGCGTCGTCAGCCCCg                                                               <  2:1991807/101‑1 (MQ=255)
                                       ggCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCg                                                               <  2:1244031/101‑1 (MQ=255)
                                       ggCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCg                                                               <  1:310574/101‑1 (MQ=255)
                                          cgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCaa                                                            <  1:3775243/101‑1 (MQ=255)
                                             cAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcg                                                         <  1:2937292/101‑1 (MQ=255)
                                              aGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgc                                                        >  2:2490774/1‑101 (MQ=255)
                                                cTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccg                                                      <  1:3733201/101‑1 (MQ=255)
                                                 tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGTGTCGTCAGCCCCGCAATcgccgc                                                     <  2:4147759/101‑1 (MQ=255)
                                                 tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccgc                                                     <  2:3881475/101‑1 (MQ=255)
                                                   cgcCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCgcgc                                                   >  2:2146638/1‑101 (MQ=255)
                                                    gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAGTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCt                                                  >  1:3651573/1‑101 (MQ=255)
                                                    gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCt                                                  <  1:4008623/101‑1 (MQ=255)
                                                     ccgagccgagCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTg                                                 >  1:602925/1‑101 (MQ=255)
                                                      cgagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTgg                                                >  2:1689342/1‑101 (MQ=255)
                                                       gagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGt                                               >  2:3022026/1‑101 (MQ=255)
                                                         gccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCa                                             <  1:121264/101‑1 (MQ=255)
                                                         gccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCa                                             >  2:178510/1‑101 (MQ=255)
                                                         gccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCa                                             <  1:3156890/101‑1 (MQ=255)
                                                         gccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCa                                             <  1:1504186/101‑1 (MQ=255)
                                                          ccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAg                                            <  2:2070862/101‑1 (MQ=255)
                                                           cgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGGGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGc                                           >  1:3502953/1‑101 (MQ=255)
                                                            gagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGcc                                          <  2:1974657/101‑1 (MQ=255)
                                                            gagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGcc                                          >  2:2347774/1‑101 (MQ=255)
                                                               cgcgGTGACCGCCGCGCGCGACAGCGCCGCGACCTGCGCCGTGCCCAGCGCCGCGCCTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcg                                       <  1:2766170/101‑1 (MQ=255)
                                                                gcgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgc                                      >  1:3822288/1‑101 (MQ=255)
                                                                 cgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgcg                                     <  1:2627346/101‑1 (MQ=255)
                                                                  ggTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCNGCGCGa                                    >  1:1757564/1‑101 (MQ=255)
                                                                   gTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGAt                                   <  1:316046/101‑1 (MQ=255)
                                                                    tGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATc                                  <  2:435767/101‑1 (MQ=255)
                                                                    tGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATc                                  <  1:1864001/101‑1 (MQ=255)
                                                                    tGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATc                                  <  1:650684/101‑1 (MQ=255)
                                                                     gACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCt                                 >  2:1022986/1‑101 (MQ=255)
                                                                         gccgcGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTgcgc                             >  2:4206942/1‑101 (MQ=255)
                                                                           cgcgCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCg                           >  1:2515670/1‑101 (MQ=255)
                                                                             cgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCGGCGCTGGTCAGCCGCGCGATCTGCGCCGTg                         <  2:2251038/101‑1 (MQ=255)
                                                                             cgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTg                         <  1:3341778/101‑1 (MQ=255)
                                                                             cgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTg                         >  1:4192706/1‑101 (MQ=255)
                                                                              gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg                        <  1:1075262/101‑1 (MQ=255)
                                                                              gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg                        <  2:621904/101‑1 (MQ=255)
                                                                              gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg                        <  2:2819962/101‑1 (MQ=255)
                                                                                tCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTc                      >  2:2129017/1‑101 (MQ=255)
                                                                                 cGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCa                     <  1:1281634/101‑1 (MQ=255)
                                                                                 cGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCa                     <  2:2886206/101‑1 (MQ=255)
                                                                                  gACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAg                    >  1:216111/1‑101 (MQ=255)
                                                                                  gACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAg                    <  2:57809/101‑1 (MQ=255)
                                                                                    cAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAggg                  <  1:1528528/101‑1 (MQ=255)
                                                                                       cgccgcGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGgcgc               <  2:2311742/101‑1 (MQ=255)
                                                                                        gccgcGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGGcgcg              <  2:1228209/101‑1 (MQ=255)
                                                                                         ccgcGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGGCGCgg             <  2:2400563/101‑1 (MQ=255)
                                                                                             gACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGGCGAGGATCt         >  1:4168328/1‑101 (MQ=255)
                                                                                              aCCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGGCGCGGATCTg        <  1:2527893/101‑1 (MQ=255)
                                                                                              aCCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGGCGCGGATCTg        <  1:1659437/101‑1 (MQ=255)
                                                                                               ccTGCGCTGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGGCGCGGATCTgc       <  2:2256533/101‑1 (MQ=255)
                                                                                                    gcCGGGCTCAGCCCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCCCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGGCGCGGATCTGCGCCGt  <  1:2146783/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAGGGCGCGGATCTGCGCCGT  >  NC_014034/678486‑678686

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG