Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,816 T→C 7.7% A1597A (GCA→GGC RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,8160T→C 7.7% 26.4 ‑3.4 ‑0.1193A1597A (GCA→GCGRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (12/3):  ref base (91/87):  total (103/90)
Fisher's exact test strand distribution p-value = 3.42e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.22e-01

Only 100 of 199 total aligned reads displayed.

CCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCGCCGCCA  >  NC_014034/678717‑678916
                                                                                                   |                                                                                                    
ccccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTg                                                                                                     >  2:27447/1‑101 (MQ=255)
 cccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgc                                                                                                    >  2:2379548/1‑101 (MQ=255)
  ccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgcc                                                                                                   >  1:1889560/1‑101 (MQ=255)
   cGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccg                                                                                                  <  2:2309026/101‑1 (MQ=255)
      cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                                                                               >  2:1781003/1‑101 (MQ=255)
      cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                                                                               <  1:2795697/101‑1 (MQ=255)
        tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc                                                                                             <  1:112285/101‑1 (MQ=255)
          gCCGAGACCGAAAGCTGGGCGGTATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcgc                                                                                           >  1:3117619/1‑101 (MQ=255)
            cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                                                         >  2:3398350/1‑101 (MQ=255)
            cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGAGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                                                         >  1:2061707/1‑101 (MQ=255)
             gagaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGt                                                                                        >  2:940483/1‑101 (MQ=255)
             gagaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGt                                                                                        <  1:2379724/101‑1 (MQ=255)
               gaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTgg                                                                                      >  1:4155873/1‑101 (MQ=255)
                 ccGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTc                                                                                    <  1:3901784/101‑1 (MQ=255)
                 ccGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTc                                                                                    >  1:1018974/1‑101 (MQ=255)
                  cGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCa                                                                                   >  2:1590637/1‑101 (MQ=255)
                     aaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcg                                                                                >  2:2882656/1‑101 (MQ=255)
                     aaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcg                                                                                >  2:276497/1‑101 (MQ=255)
                      aGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcgc                                                                               >  2:2457139/1‑101 (MQ=255)
                       gCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGcc                                                                              <  2:2512200/101‑1 (MQ=255)
                        ccgcaccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCa                                                                             >  2:3187700/10‑101 (MQ=2)
                        cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCa                                                                             <  1:3012223/101‑1 (MQ=255)
                         tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCAc                                                                            >  2:4096891/1‑101 (MQ=255)
                         tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCAc                                                                            <  2:3476032/101‑1 (MQ=255)
                         cgcaccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                                            >  1:3879315/9‑101 (MQ=2)
                          gggCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACg                                                                           >  1:1509311/1‑101 (MQ=255)
                           caccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                                                                          >  1:3456857/7‑101 (MQ=2)
                            gCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGAt                                                                         <  1:780670/101‑1 (MQ=255)
                             cGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATg                                                                        >  1:910768/1‑101 (MQ=255)
                               agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                                      <  1:351247/99‑1 (MQ=2)
                               agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                                      <  1:3356312/99‑1 (MQ=2)
                                tatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCa                                                                     <  1:1772011/101‑1 (MQ=255)
                                tatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCa                                                                     <  1:3789185/101‑1 (MQ=255)
                                gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                                     >  2:1319578/2‑101 (MQ=2)
                                 atCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                                                    >  1:328639/1‑101 (MQ=2)
                                 atCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                                                    >  2:3619142/1‑101 (MQ=2)
                                  tCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAtt                                                                   <  1:232575/101‑1 (MQ=255)
                                   cggcgGTTTCCAGCGCCGCAACCTGCGTGCTGGCCAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAtcg                                                                  <  2:3517112/99‑3 (MQ=9)
                                   cggcgGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAtcg                                                                  >  1:4226183/3‑99 (MQ=9)
                                   ccGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTc                                                                  >  1:21619/1‑101 (MQ=255)
                                     gcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGt                                                                >  1:3052498/1‑101 (MQ=255)
                                       ggTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGc                                                              <  1:455407/101‑1 (MQ=255)
                                          ttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCa                                                           <  2:1707634/101‑1 (MQ=255)
                                          ttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCa                                                           <  2:115550/101‑1 (MQ=255)
                                           tCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAg                                                          <  1:353232/101‑1 (MQ=255)
                                             acatgccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCCCGATGTCATTCGTGCCCAGcc                                                        >  2:4216716/5‑101 (MQ=255)
                                              aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                                                       <  2:3155752/101‑1 (MQ=255)
                                              aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                                                       >  1:1438329/1‑101 (MQ=255)
                                                cgccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCACAGCCCCt                                                     <  1:1343587/101‑1 (MQ=255)
                                                   cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa                                                  <  1:2457323/101‑1 (MQ=255)
                                                   cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa                                                  <  1:3733733/101‑1 (MQ=255)
                                                    gcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAg                                                 <  2:3142826/101‑1 (MQ=255)
                                                    gcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAg                                                 <  1:2597313/101‑1 (MQ=255)
                                                     cgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgc                                                <  2:193978/101‑1 (MQ=255)
                                                      gATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcg                                               >  1:1557984/1‑101 (MQ=255)
                                                       aTCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcgc                                              >  2:76249/1‑101 (MQ=255)
                                                       aTCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcgc                                              >  2:1294112/1‑101 (MQ=255)
                                                        tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc                                             <  1:27461/101‑1 (MQ=255)
                                                         cTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgccg                                            >  2:3113334/1‑101 (MQ=255)
                                                         cTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgccg                                            >  1:3167477/1‑101 (MQ=255)
                                                         cTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgccg                                            <  1:4030391/101‑1 (MQ=255)
                                                            cgcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCgg                                         <  2:2973405/101‑1 (MQ=255)
                                                             gcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGt                                        >  2:3832024/1‑101 (MQ=255)
                                                             gcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGt                                        <  2:913460/101‑1 (MQ=255)
                                                             gcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGt                                        <  1:3954036/101‑1 (MQ=255)
                                                                gtccccAGCGCCGCGTCCTCGGCCACGCTCGGCCGTGCCGCCGGCGCAGTGGTCGGCGCCACGATGTCATTCGTCCCCAGCCCCTTCAGCGCCCCGGTGCt                                     <  2:1120862/96‑1 (MQ=255)
                                                                gTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCt                                     <  2:262670/101‑1 (MQ=255)
                                                                  gCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCa                                   <  1:2668059/101‑1 (MQ=255)
                                                                  gCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCa                                   <  2:229411/101‑1 (MQ=255)
                                                                   cTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAg                                  >  1:550853/1‑101 (MQ=255)
                                                                    tCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCacc                                 >  1:4085673/1‑99 (MQ=255)
                                                                     cAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAgcg                                <  2:3028646/101‑1 (MQ=255)
                                                                       gcgcCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAgcgcg                              <  1:1956133/101‑1 (MQ=255)
                                                                       gcgcCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAgcgcg                              <  1:3235947/101‑1 (MQ=255)
                                                                         gccgcGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGcc                            >  2:599093/1‑101 (MQ=255)
                                                                         gccgcGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGcc                            >  2:3301915/1‑101 (MQ=255)
                                                                         gccgcGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGcc                            <  2:2236854/101‑1 (MQ=255)
                                                                          ccgcGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCg                           <  1:2486791/101‑1 (MQ=255)
                                                                             cgttCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATc                        <  2:550789/98‑1 (MQ=255)
                                                                              gATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCg                       >  2:3133217/1‑101 (MQ=255)
                                                                               aTCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGc                      <  2:676991/101‑1 (MQ=255)
                                                                               aTCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGc                      >  1:932066/1‑101 (MQ=255)
                                                                                tCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcc                     >  2:839598/1‑101 (MQ=255)
                                                                                tCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcc                     <  2:2326061/101‑1 (MQ=255)
                                                                                 ctgctccgcgCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGccc                    <  1:1590746/95‑1 (MQ=255)
                                                                                  tGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcccc                   <  2:2539080/101‑1 (MQ=255)
                                                                                    cGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCgg                 <  2:1657511/101‑1 (MQ=255)
                                                                                      tCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTc               <  1:690113/101‑1 (MQ=255)
                                                                                      tCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTc               <  2:270167/101‑1 (MQ=255)
                                                                                       cgcgCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCg              >  1:1072340/1‑101 (MQ=255)
                                                                                          gcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCGACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACa           <  1:3659015/101‑1 (MQ=255)
                                                                                           cTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAg          >  1:2510444/1‑101 (MQ=255)
                                                                                            tCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCCGCGCCGCGGCGGTCAGCACGCCGACCGCCCCCGGCGTCAgc         >  2:1749114/1‑101 (MQ=25)
                                                                                            tCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgc         >  1:294519/1‑101 (MQ=255)
                                                                                             cAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgcg        <  2:1438227/101‑1 (MQ=255)
                                                                                             cAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGANCGCCCCGGTCGACAgcg        >  2:199713/1‑101 (MQ=255)
                                                                                              aGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgcgc       >  2:2954074/1‑101 (MQ=255)
                                                                                               gCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGcgcc      >  2:1427555/1‑101 (MQ=255)
                                                                                                  gTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCgccgcc   <  1:1686612/101‑1 (MQ=255)
                                                                                                   tGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCgccgccc  >  2:1223168/1‑100 (MQ=255)
                                                                                                   |                                                                                                    
CCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCGCCGCCA  >  NC_014034/678717‑678916

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG