BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	681,149	T→G	9.7%	M820L (ATC→CTC)	RCAP_rcc00630 ←	ice nucleation protein repeat family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	681,149	0	T→G	9.7%	22.2 ‑3.2 ‑0.7	103	M820L (ATC→CTC)	RCAP_rcc00630	ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand): new base (9/1): ref base (50/43): total (59/44)
Fisher's exact test strand distribution p-value = 4.08e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.95e-01

Only 100 of 106 total aligned reads displayed.


AGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCA  >  NC_014034/681052‑681249


aGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGc                                                                                                   >  1:1506451/1‑101 (MQ=25)


aGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGc                                                                                                   >  1:3916708/1‑101 (MQ=25)


   tGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcc                                                                                                >  1:2103216/1‑98 (MQ=25)


   tGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcc                                                                                                >  1:3539066/1‑98 (MQ=25)


    gcgcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcca                                                                                               >  2:3074367/1‑97 (MQ=25)


     cgcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccac                                                                                              >  1:2113462/1‑96 (MQ=25)


     cgcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccaa                                                                                              >  2:2617187/1‑96 (MQ=25)


      cccttGCTCAGCGCCGCCACCTGCTGCGAGGTTATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccact                                                                                             <  1:1625932/97‑7 (MQ=21)


       ccGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGctccactg                                                                                            >  1:3933321/1‑94 (MQ=25)


       ccGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactg                                                                                            >  1:3101068/1‑94 (MQ=25)


         gTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggc                                                                                          >  1:2777898/1‑92 (MQ=21)


                                                   tgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgt                                                <  1:503909/92‑1 (MQ=38)


                                                    gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                                               <  1:520833/93‑1 (MQ=255)


                                                    gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                                               <  2:1253766/93‑1 (MQ=255)


                                                     cgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcg                                              >  1:2463190/8‑101 (MQ=255)


                                                      gccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcgt                                             >  1:2199396/7‑101 (MQ=255)


                                                       ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg                                            <  2:1750863/96‑1 (MQ=255)


                                                       ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg                                            <  1:2791572/96‑1 (MQ=255)


                                                        cgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGc                                           >  2:570188/5‑101 (MQ=255)


                                                         gtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCt                                          >  2:4012875/4‑101 (MQ=255)


                                                         gtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCt                                          <  1:2684716/98‑1 (MQ=255)


                                                         gtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGCCGTGCt                                          >  1:3482095/4‑101 (MQ=255)


                                                         gtcgTCAGCGCCACGATGTCAGCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCt                                          >  1:3118963/4‑101 (MQ=255)


                                                          tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                                         <  1:3109555/99‑1 (MQ=255)


                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        <  1:1727738/100‑1 (MQ=255)


                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        <  2:3768971/100‑1 (MQ=255)


                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        <  2:3173425/100‑1 (MQ=255)


                                                            gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                                       <  2:2268405/101‑1 (MQ=255)


                                                            gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                                       >  2:724654/1‑101 (MQ=255)


                                                            gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                                       >  2:386776/1‑101 (MQ=255)


                                                             tCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc                                      <  1:188795/101‑1 (MQ=255)


                                                             gcAGCGCCACGATGTCATCGGTGCCGAGCCCCTTGGTCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCGGCGTCGTGCTCAgc                                      <  2:2476925/100‑1 (MQ=38)


                                                              cAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcg                                     >  1:2965703/1‑101 (MQ=255)


                                                               aGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc                                    <  1:116740/101‑1 (MQ=255)


                                                               aGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc                                    >  2:3045110/1‑101 (MQ=255)


                                                                gcgcGACGGTGTCATGGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGcgcg                                   <  2:1124803/101‑1 (MQ=255)


                                                                 cgccACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCgg                                  <  2:1116459/101‑1 (MQ=255)


                                                                  gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGc                                 >  1:4196210/4‑101 (MQ=255)


                                                                    cacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCa                               >  2:621098/2‑101 (MQ=255)


                                                                    cacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCa                               <  2:2006800/100‑1 (MQ=255)


                                                                    cacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCa                               >  1:3998391/2‑101 (MQ=255)


                                                                     acgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCAt                              >  2:3379531/1‑101 (MQ=255)


                                                                     acgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCAt                              >  1:1402418/1‑101 (MQ=255)


                                                                      cgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATc                             >  1:3538536/1‑101 (MQ=255)


                                                                      cgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATc                             >  1:1690078/1‑101 (MQ=255)


                                                                      cgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATc                             <  1:2779193/101‑1 (MQ=255)


                                                                       gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            >  2:3726913/1‑101 (MQ=255)


                                                                       gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            >  1:1719755/1‑101 (MQ=255)


                                                                       gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            >  2:49605/1‑101 (MQ=255)


                                                                       gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            <  2:1090733/101‑1 (MQ=255)


                                                                       gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            >  1:3675456/1‑101 (MQ=255)


                                                                       gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            <  2:2965481/101‑1 (MQ=255)


                                                                        aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg                           <  2:677734/101‑1 (MQ=255)


                                                                        aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg                           <  2:3341033/101‑1 (MQ=255)


                                                                        aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg                           <  1:48190/101‑1 (MQ=255)


                                                                        aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg                           <  1:3953337/101‑1 (MQ=255)


                                                                        aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg                           <  2:2829093/101‑1 (MQ=255)


                                                                         tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc                          >  1:3131693/1‑101 (MQ=255)


                                                                         tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc                          >  2:2621255/1‑101 (MQ=255)


                                                                         tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc                          >  2:1060578/1‑101 (MQ=255)


                                                                         tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc                          >  2:2774708/1‑101 (MQ=255)


                                                                          gTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcg                         >  1:4051541/1‑101 (MQ=255)


                                                                           tCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcgc                        <  1:74513/101‑1 (MQ=255)


                                                                           tCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcgc                        <  1:2715068/101‑1 (MQ=255)


                                                                            cATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGcc                       <  1:2788530/101‑1 (MQ=255)


                                                                               cGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    <  1:1618409/101‑1 (MQ=255)


                                                                                ggTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAgg                   >  2:3039916/1‑101 (MQ=255)


                                                                                 gTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGt                  >  2:2406040/1‑101 (MQ=255)


                                                                                 gTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGt                  >  1:1052388/1‑101 (MQ=255)


                                                                                  tGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTc                 <  1:2774927/101‑1 (MQ=255)


                                                                                  tGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTc                 <  1:2921293/101‑1 (MQ=255)


                                                                                   gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCa                <  1:4077471/101‑1 (MQ=255)


                                                                                   gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCa                <  1:3727167/101‑1 (MQ=255)


                                                                                    ccGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAg               >  2:2060520/1‑101 (MQ=255)


                                                                                    ccGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAg               <  2:48631/101‑1 (MQ=255)


                                                                                    ccGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAg               <  2:1306097/101‑1 (MQ=255)


                                                                                    ccGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAg               <  1:3743113/101‑1 (MQ=255)


                                                                                    ccGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAg               >  1:3395833/1‑101 (MQ=255)


                                                                                    ccGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAg               >  2:2979530/1‑101 (MQ=255)


                                                                                     cGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGc              >  2:2519923/1‑101 (MQ=255)


                                                                                      gAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             >  1:3030436/1‑101 (MQ=255)


                                                                                      gAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             >  1:1037926/1‑101 (MQ=255)


                                                                                      gAGGCCCTTGATCGCATTGGCACTCAGCGCAGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             <  2:3481843/101‑1 (MQ=255)


                                                                                       aGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGccc            >  1:302570/1‑101 (MQ=255)


                                                                                       aGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGccc            >  2:3989360/1‑101 (MQ=255)


                                                                                         gCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgc          >  1:335570/1‑101 (MQ=255)


                                                                                         gCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgc          <  2:482122/101‑1 (MQ=255)


                                                                                             ttGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAc      >  1:3978133/1‑101 (MQ=255)


                                                                                             ttGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAc      >  2:1303523/1‑101 (MQ=255)


                                                                                             ttGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAc      >  1:1321254/1‑101 (MQ=255)


                                                                                             ttGATCGCATTGGCACTCAGCCCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGACGTGCTCAGCGCGGCCATCTGCGCCGAGCTCAGCACGGGCAc      >  2:1359544/1‑101 (MQ=255)


                                                                                              tGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCTTGCTCAGCGCGGCCATCTGCGCCGAGGCCAGCCCGCGCAcc     >  2:2727871/1‑101 (MQ=255)


                                                                                              tGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAcc     >  1:3225269/1‑101 (MQ=255)


                                                                                              tGATCGCATTGGCACTCAGCGCCGTCACGCAGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAcc     >  1:3769771/1‑101 (MQ=255)


                                                                                               gATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCg    <  2:1052294/101‑1 (MQ=255)


                                                                                               gATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCg    >  2:585312/1‑101 (MQ=255)


                                                                                                aTCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGc   >  1:1803682/1‑101 (MQ=255)


                                                                                                aTCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGc   <  1:2012765/101‑1 (MQ=255)


                                                                                                 tCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCa  <  1:4013135/101‑1 (MQ=255)


                                                                                                 tCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCa  <  1:2241751/101‑1 (MQ=255)


AGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCA  >  NC_014034/681052‑681249

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG