Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,666 G→C 13.4% L647L (CTC→GCC RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,6660G→C13.4% 54.9 ‑2.6 ‑0.0178L647L (CTC→CTGRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (15/9):  ref base (64/90):  total (79/99)
Fisher's exact test strand distribution p-value = 7.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.95e-01

Only 100 of 196 total aligned reads displayed.

TGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCG  >  NC_014034/681566‑681766
                                                                                                    |                                                                                                    
tGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgag                                                                                                      <  2:3379102/101‑1 (MQ=255)
 gCCCAGGGCCGCCACCGTCGCCGAAGCCAGGGCCGCGATCTGCGCCGTGCTCAGCGCGGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgaga                                                                                                     <  2:1279234/101‑1 (MQ=255)
 gCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgaga                                                                                                     <  2:1367173/101‑1 (MQ=255)
 gCCCAGCGCCCCCACCGTGGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgaga                                                                                                     <  2:1047108/101‑1 (MQ=255)
   cgaGCGCCGCCGCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCCCCTGCCCCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgc                                                                                                   <  2:4166607/99‑1 (MQ=255)
     aGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAtcgc                                                                                                 <  2:3855715/101‑1 (MQ=255)
      gcgcCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcgcg                                                                                                >  2:2910587/1‑101 (MQ=255)
        gccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGcc                                                                                              >  1:4116315/1‑101 (MQ=255)
        gccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGcc                                                                                              <  1:2858596/101‑1 (MQ=255)
         ccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCg                                                                                             <  2:971818/101‑1 (MQ=255)
          cgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGCCTGGAGAGCGCGCCGa                                                                                            >  2:2036020/1‑101 (MQ=255)
           gccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAc                                                                                           >  1:1006707/1‑101 (MQ=255)
           gccACCGTCGCCGAAGCCAGCGCCGAGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAc                                                                                           >  2:1578068/1‑101 (MQ=255)
            ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCCCCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAcc                                                                                          <  1:4196511/101‑1 (MQ=255)
             cACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCt                                                                                         <  2:3675123/101‑1 (MQ=255)
             cACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCt                                                                                         <  2:2691286/101‑1 (MQ=255)
               ccGTCGCGGAGGCGAGCGCCGTGATCTGCGCTGTGCGCAGCGCCGCCGCCTGAACCGAGCCAAGCGCGCCCAGCGCAAGGCTGGAGAGCGCGCCGACCTGa                                                                                       <  2:3951457/101‑1 (MQ=255)
               ccGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGa                                                                                       <  1:3894161/101‑1 (MQ=255)
                 gTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATc                                                                                     <  2:2136168/101‑1 (MQ=255)
                     gggtggaaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTg                                                                                 <  1:1115268/91‑1 (MQ=0)
                       gtggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGcc                                                                               >  1:3398693/9‑100 (MQ=2)
                       gAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCt                                                                               >  2:3212533/1‑101 (MQ=255)
                        tggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGccc                                                                              >  1:1375244/8‑99 (MQ=2)
                        tggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGccc                                                                              >  2:2847620/8‑99 (MQ=2)
                        aaGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTc                                                                              >  1:2446413/1‑101 (MQ=255)
                         ggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                             <  1:443267/95‑1 (MQ=2)
                         ggcaatcgccgcGATCTGCCCCGCGCTCAGCCCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                             <  1:3213456/95‑1 (MQ=2)
                         aGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCa                                                                             <  2:357191/101‑1 (MQ=255)
                          gCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAg                                                                            >  1:1321171/1‑101 (MQ=255)
                          gCCAGCGCCGCGATCTGCGCCGTGCTCAGCCCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTTAg                                                                            <  1:239802/101‑1 (MQ=255)
                           caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                           >  1:858780/5‑100 (MQ=2)
                           caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                           >  1:1901590/5‑100 (MQ=2)
                           caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                           >  2:4229225/5‑100 (MQ=2)
                            cAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCt                                                                          <  2:3189532/101‑1 (MQ=255)
                            cAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCt                                                                          >  1:2593372/1‑101 (MQ=255)
                            aatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                          >  1:3433027/4‑99 (MQ=2)
                            aaGCGCAGAGATCTGAGCCGGGCAGCGCGCGGCCGCGTACACTAAACCAAGCCCGCCCACCGCAAGGCTGGAGAGCTCGCCGACCTGATCGGTGCTCAGCt                                                                          <  2:2956129/100‑1 (MQ=255)
                              gcgcCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTg                                                                        <  2:58803/101‑1 (MQ=255)
                               cgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGACCAGCGCCGCGACCTGATCGGTGCCCAgactgg                                                                       >  1:3610598/1‑96 (MQ=2)
                               ccccgcGATCTGCGCCGCGCTCGGCGCCGCGATCGGCGCGGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactgg                                                                       <  2:2267179/99‑6 (MQ=2)
                                gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                      >  2:2528702/1‑101 (MQ=255)
                                gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                      <  2:1599639/101‑1 (MQ=255)
                                gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                      <  1:2827358/101‑1 (MQ=255)
                                gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                      >  1:92791/1‑101 (MQ=255)
                                gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCAAAGCCCGGCCACCGCCAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                      >  2:4073881/1‑101 (MQ=255)
                                  cgcgATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGa                                                                    >  1:74805/1‑101 (MQ=255)
                                  cgcgATCTGCGCCGCGCGCAGCGCCGCGATCTGCGCGGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                                    <  2:3477803/101‑9 (MQ=2)
                                    cgATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATc                                                                  >  1:3185735/1‑101 (MQ=255)
                                     gATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCt                                                                 <  2:2148809/101‑1 (MQ=255)
                                     gATCGGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCt                                                                 <  2:4102107/101‑1 (MQ=255)
                                      aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                <  1:261799/101‑1 (MQ=255)
                                       tCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                               >  2:1963554/1‑101 (MQ=255)
                                       tCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                               <  1:2501625/101‑1 (MQ=255)
                                        cTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCg                                                              >  1:725277/1‑101 (MQ=255)
                                        cTGCGCCGTGCTCAGCCCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCg                                                              <  2:3332897/101‑1 (MQ=255)
                                          gcgcCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTc                                                            <  2:1895886/101‑1 (MQ=255)
                                                tGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTc                                                      >  1:643976/1‑101 (MQ=255)
                                                tGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTc                                                      <  2:847004/101‑1 (MQ=255)
                                                  cTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAg                                                    <  1:182599/101‑1 (MQ=255)
                                                  cTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAg                                                    >  1:3925254/1‑101 (MQ=255)
                                                   tCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAGACTGGCCAGCTGCATCGTGCCCAgc                                                   >  2:2651853/1‑100 (MQ=0)
                                                   tCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAgg                                                   >  2:2096260/1‑101 (MQ=255)
                                                    cAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGc                                                  >  1:503272/1‑101 (MQ=255)
                                                     aGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGcc                                                 >  1:4146552/1‑101 (MQ=255)
                                                      gcgcCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGccc                                                <  1:1069634/101‑1 (MQ=255)
                                                       cgccgccgcCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCg                                               <  1:3332894/101‑1 (MQ=255)
                                                       cgccgccgcCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCg                                               >  1:2997330/1‑101 (MQ=255)
                                                        gccgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGa                                              <  2:2998060/101‑1 (MQ=255)
                                                        gccgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGa                                              <  1:2910806/101‑1 (MQ=255)
                                                          cgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGaaa                                            <  1:1414177/101‑1 (MQ=255)
                                                           gccgccTCCACCGAGCCAAGCCCGCCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAc                                           <  2:552308/101‑1 (MQ=255)
                                                            ccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAcc                                          <  1:2149336/101‑1 (MQ=255)
                                                            ccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAcc                                          >  2:2351609/1‑101 (MQ=255)
                                                            ccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAcc                                          <  2:1321071/101‑1 (MQ=255)
                                                            ccgccGGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAcc                                          <  1:1258183/101‑1 (MQ=255)
                                                             cgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccg                                         <  1:2773466/101‑1 (MQ=255)
                                                              gccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgc                                        >  1:2003087/1‑101 (MQ=255)
                                                              gccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgc                                        >  1:81650/1‑101 (MQ=255)
                                                                cTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgccg                                      <  1:2418572/101‑1 (MQ=255)
                                                                cTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgccg                                      >  1:2466505/1‑101 (MQ=255)
                                                                   cACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCgcgc                                   >  2:468369/1‑101 (MQ=255)
                                                                         gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGcccc                             >  2:3062738/1‑100 (MQ=255)
                                                                         gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                             <  1:895307/101‑1 (MQ=255)
                                                                         gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                             <  1:1568615/101‑1 (MQ=255)
                                                                         gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                             <  2:2885003/101‑1 (MQ=255)
                                                                          ccAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAg                            <  2:817931/101‑1 (MQ=255)
                                                                          ccAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAg                            <  1:2697455/101‑1 (MQ=255)
                                                                           cAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGc                           >  2:1081535/1‑101 (MQ=255)
                                                                            aaGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcc                          <  2:2473775/101‑1 (MQ=255)
                                                                            aaGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACAGCCGCGCTGCCCAGcc                          >  2:1066421/1‑101 (MQ=255)
                                                                              gCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcccc                        <  1:2200595/101‑1 (MQ=255)
                                                                              gCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcccc                        <  2:2367566/101‑1 (MQ=255)
                                                                                     cACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCt                 <  2:2600474/101‑1 (MQ=255)
                                                                                      aCCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTg                <  1:2359268/101‑1 (MQ=255)
                                                                                           aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg           >  2:1401504/1‑101 (MQ=255)
                                                                                              gCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGc        >  2:2796502/1‑101 (MQ=255)
                                                                                                tGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGccc      <  1:2528886/101‑1 (MQ=255)
                                                                                                 ggAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCa     <  2:156219/101‑1 (MQ=255)
                                                                                                  gagaGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAACCCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAg    <  1:2351777/101‑1 (MQ=255)
                                                                                                    gagCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCg  <  2:503215/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
TGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCG  >  NC_014034/681566‑681766

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG