Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 682,546 G→T 10.3% A354E (GCG→GAG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034682,5460G→T10.3% 48.5 ‑1.7 ‑0.1211A354E (GCG→GAG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (7/15):  ref base (93/96):  total (100/111)
Fisher's exact test strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.43e-01

Only 100 of 227 total aligned reads displayed.

CTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGC  >  NC_014034/682446‑682645
                                                                                                    |                                                                                                    
cTGCGCCGAATTCAGCGCCTCGGCGCCCCCCTCGGACACCGCCGCGACCCGGCGCGCGACCAGAACCGCCAGCGGGGCCGCGCTCAGCGCCGCCATCTgcg                                                                                                      >  2:1872579/1‑101 (MQ=255)
  gcgcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGcc                                                                                                    <  2:1490981/101‑1 (MQ=255)
   cgcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGccc                                                                                                   <  2:357811/101‑1 (MQ=255)
    gcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGcccc                                                                                                  >  2:2332909/1‑101 (MQ=255)
     ccGACCTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCg                                                                                                 <  1:1673849/101‑1 (MQ=255)
     ccGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCg                                                                                                 <  1:3056308/101‑1 (MQ=255)
       gAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGt                                                                                               >  1:721866/1‑101 (MQ=255)
       gAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGt                                                                                               >  2:2852252/1‑101 (MQ=255)
        aaCTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTc                                                                                              <  1:3099557/101‑1 (MQ=255)
        aaCTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTc                                                                                              <  1:3702442/101‑1 (MQ=255)
         aCTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCa                                                                                             >  2:3158842/1‑101 (MQ=255)
          cTCAGCGCTTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCTCCCCGGTCAg                                                                                            <  1:3657294/101‑1 (MQ=255)
          cTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAg                                                                                            <  1:1707407/101‑1 (MQ=255)
           tCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgc                                                                                           <  1:4063505/101‑1 (MQ=255)
           tCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgc                                                                                           >  2:2252562/1‑101 (MQ=255)
           cgatctccTCGGCGGCCTCCTCGGACAGCGCAGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgc                                                                                           <  2:1666926/95‑1 (MQ=255)
            cAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgcg                                                                                          <  1:683293/101‑1 (MQ=255)
               cgcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgccg                                                                                       <  1:517371/101‑1 (MQ=255)
                 ccTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgcagatcg                                                                                     >  1:1667041/1‑95 (MQ=255)
                  cTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGACCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCa                                                                                    <  2:3496690/101‑1 (MQ=255)
                   tCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAg                                                                                   >  1:3739751/1‑101 (MQ=255)
                   tCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAg                                                                                   <  1:999819/101‑1 (MQ=255)
                     ggcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGtt                                                                                 >  2:323095/1‑101 (MQ=255)
                     ggcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGtt                                                                                 >  1:2526746/1‑101 (MQ=255)
                     ggcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGtt                                                                                 <  2:256035/101‑1 (MQ=255)
                       cggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCg                                                                               >  2:878711/1‑101 (MQ=255)
                       cggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCg                                                                               >  2:4030975/1‑101 (MQ=255)
                        ggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGa                                                                              >  2:2363093/1‑101 (MQ=255)
                        ggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGa                                                                              >  1:3412494/1‑101 (MQ=255)
                         gcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGAt                                                                             >  1:1110574/1‑101 (MQ=255)
                          cctcctCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATc                                                                            <  2:717860/101‑1 (MQ=255)
                              ctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGc                                                                       <  2:209198/101‑1 (MQ=255)
                              ctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGc                                                                       >  1:2581045/1‑101 (MQ=255)
                                        gccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGc                                                             >  2:3919633/1‑101 (MQ=255)
                                         ccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGcc                                                            <  1:2494557/101‑1 (MQ=255)
                                         ccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGcc                                                            <  2:3818818/101‑1 (MQ=255)
                                           gcgATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCAt                                                          >  2:214766/1‑101 (MQ=255)
                                           gcgAGCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCAt                                                          <  2:1307656/101‑1 (MQ=255)
                                               tCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTcc                                                      <  1:2335302/101‑1 (MQ=255)
                                                 tGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTc                                                    <  1:1363589/101‑1 (MQ=255)
                                                 tGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTc                                                    <  2:1196800/101‑1 (MQ=255)
                                                  gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                                                   <  2:2409724/101‑1 (MQ=255)
                                                  gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                                                   >  2:2718780/1‑101 (MQ=255)
                                                    cgcgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGt                                                 >  1:605805/1‑101 (MQ=255)
                                                      cgTGCCCAGCACCCCCAGCTGGGCCGTGCGCAGCGCCCCCTTCTGCCCCCCGGTCCGCGCCGCCAGTTCGATCG.TGCTCCGCCCGGCCATGTCCTCGGTCg                                               <  1:1277558/101‑1 (MQ=255)
                                                      cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                                               <  1:2192278/101‑1 (MQ=255)
                                                       gTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGa                                              >  1:2826145/1‑101 (MQ=255)
                                                       gTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCACCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGa                                              >  2:1950742/1‑101 (MQ=255)
                                                         gCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaaa                                            >  2:3872567/1‑101 (MQ=255)
                                                          cccAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGTTCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAg                                           <  1:1039325/101‑1 (MQ=255)
                                                          cccAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAg                                           <  1:323140/101‑1 (MQ=255)
                                                            cAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcc                                         >  1:41459/1‑101 (MQ=255)
                                                            cAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcc                                         <  2:3369387/101‑1 (MQ=255)
                                                             aGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGccc                                        <  2:1149635/101‑1 (MQ=255)
                                                              gAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCAGGCCATGTCCTCGGTCGAAAGcccc                                       >  1:3910702/1‑101 (MQ=255)
                                                               cgccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCt                                      >  1:1014608/6‑101 (MQ=37)
                                                               aaCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCt                                      <  1:4204687/101‑1 (MQ=255)
                                                               aaCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCt                                      >  2:3423476/1‑101 (MQ=255)
                                                                gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt                                     <  2:4224713/97‑1 (MQ=37)
                                                                aCCCCCAGCTGGGCCGTTCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCtt                                     <  2:4183319/101‑1 (MQ=255)
                                                                 cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc                                    >  2:3064631/1‑101 (MQ=255)
                                                                 cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc                                    >  1:4012712/1‑101 (MQ=255)
                                                                 cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc                                    <  1:2451348/101‑1 (MQ=255)
                                                                  cgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttga                                   >  2:1115174/3‑99 (MQ=37)
                                                                  ccccGGCGGGGCCGTGCTCAGCCCCGCCATCTGCGCCCCGGTCACCGCAGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa                                   <  2:2791736/101‑1 (MQ=255)
                                                                  ccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa                                   <  2:2601613/101‑1 (MQ=255)
                                                                   gccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAg                                  <  1:1610884/100‑1 (MQ=25)
                                                                   cccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAg                                  >  1:820830/1‑101 (MQ=255)
                                                                    ccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgc                                 >  1:2916034/1‑101 (MQ=255)
                                                                     cAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgcg                                <  2:3446432/101‑1 (MQ=37)
                                                                     cAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgcg                                >  1:3064618/1‑101 (MQ=25)
                                                                      aGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc                               <  2:563317/101‑1 (MQ=255)
                                                                       gCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGcgcg                              >  1:743545/1‑101 (MQ=255)
                                                                        cTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcgg                             >  2:3209454/1‑101 (MQ=255)
                                                                         tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc                            >  1:7571/1‑101 (MQ=255)
                                                                         tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc                            <  1:3560623/101‑1 (MQ=255)
                                                                         tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc                            >  1:2261773/1‑101 (MQ=255)
                                                                         tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTCCAGCgcggc                            >  1:984641/1‑101 (MQ=255)
                                                                          gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg                           <  1:408044/101‑1 (MQ=255)
                                                                          gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg                           <  2:1510979/101‑1 (MQ=255)
                                                                          gcgCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGcgcgcca                           <  1:2657713/99‑4 (MQ=25)
                                                                            gCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGt                         >  2:920311/1‑101 (MQ=255)
                                                                             ccGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTg                        <  1:357660/101‑1 (MQ=255)
                                                                              cGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGcgcgccactgg                       <  1:1931676/101‑8 (MQ=25)
                                                                               gTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGcc                      <  2:3727123/101‑1 (MQ=255)
                                                                                 gCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGa                    >  1:118393/1‑101 (MQ=255)
                                                                                 gCTAAGCGCGGCCATCTGCGCCCCGGCCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGa                    >  2:2066740/1‑101 (MQ=255)
                                                                                   tCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATc                  >  2:3927351/1‑101 (MQ=255)
                                                                                    cAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcg                 <  1:1295052/101‑1 (MQ=255)
                                                                                     aGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgc                >  2:585448/1‑101 (MQ=255)
                                                                                       cgccgcCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccg              <  1:3175151/101‑1 (MQ=255)
                                                                                       cgccgcCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccg              <  1:214804/101‑1 (MQ=255)
                                                                                        gccgccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccgc             <  2:2519403/101‑1 (MQ=255)
                                                                                          cgccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaa           >  2:2953597/1‑101 (MQ=255)
                                                                                           gccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaaa          <  1:1893747/101‑1 (MQ=255)
                                                                                           gccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaaa          <  1:1946186/101‑1 (MQ=255)
                                                                                            ccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAg         >  2:1109608/1‑101 (MQ=255)
                                                                                            ccATCTGCGCCCCGGGCAGCGACGCCAGTTGGATCG.TACTCGTCCCGGCCATGTACTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAg         <  2:1683808/101‑1 (MQ=255)
                                                                                                 tGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCcgc    <  2:79484/101‑1 (MQ=255)
                                                                                                  gcgcCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCcgcg   <  1:1313274/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGC  >  NC_014034/682446‑682645

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG