Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 682,556 G→T 10.6% M351M (CTG→ATG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034682,5560G→T10.6% 46.4 ‑1.8 ‑0.1198M351M (CTG→ATG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (7/14):  ref base (90/87):  total (97/101)
Fisher's exact test strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.87e-01

Only 100 of 218 total aligned reads displayed.

CTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCG  >  NC_014034/682456‑682656
                                                                                                    |                                                                                                     
cTCAGCGCTTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCTCCCCGGTCAg                                                                                                       <  1:3657294/101‑1 (MQ=255)
 tCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgc                                                                                                      >  2:2252562/1‑101 (MQ=255)
 cgatctccTCGGCGGCCTCCTCGGACAGCGCAGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgc                                                                                                      <  2:1666926/95‑1 (MQ=255)
  cAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgcg                                                                                                     <  1:683293/101‑1 (MQ=255)
      gcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgccgc                                                                                                 <  2:3094571/101‑1 (MQ=255)
       ccTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgcagatcg                                                                                                >  1:1667041/1‑95 (MQ=255)
       ccTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCgccgcc                                                                                                <  1:753992/101‑1 (MQ=255)
        cTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGACCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCa                                                                                               <  2:3496690/101‑1 (MQ=255)
         tCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAg                                                                                              >  1:1196894/1‑101 (MQ=255)
         tCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAg                                                                                              <  1:999819/101‑1 (MQ=255)
          cggcggCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGt                                                                                             >  1:628261/1‑101 (MQ=255)
          cggcggCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCC..AACACCCAGCTGTGGCGCGCGCAGCGCCCCCACATGCGCCCCGGGCAGcgccgcgcgatc                                                                                           >  2:1894083/1‑95 (MQ=255)
           ggcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGtt                                                                                            >  1:2088155/1‑101 (MQ=255)
           ggcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGtt                                                                                            >  2:323095/1‑101 (MQ=255)
           ggcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGtt                                                                                            >  1:2526746/1‑101 (MQ=255)
            gcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTc                                                                                           >  1:2355244/1‑101 (MQ=255)
             cggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCg                                                                                          >  1:3727377/1‑101 (MQ=255)
             cggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCg                                                                                          >  2:4030975/1‑101 (MQ=255)
              ggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGa                                                                                         >  2:2363093/1‑101 (MQ=255)
              ggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGa                                                                                         >  1:3900876/1‑101 (MQ=255)
              ggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGa                                                                                         <  1:4121592/101‑1 (MQ=255)
                cctcctCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATc                                                                                       <  2:717860/101‑1 (MQ=255)
                   cctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.Tg                                                                                   <  2:1123069/101‑1 (MQ=255)
                    ctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGc                                                                                  >  1:2581045/1‑101 (MQ=255)
                      cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                                                                >  2:2534681/1‑101 (MQ=255)
                            gcgcCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCg                                                                          <  2:1278289/101‑1 (MQ=255)
                             cgccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCgg                                                                         >  1:131395/1‑101 (MQ=255)
                              gtcgcgATCTGCCGCGTGCCCAGAGCGCCCAGCTGGGCCCTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGc                                                                        <  2:4205887/99‑1 (MQ=255)
                              gccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGc                                                                        >  2:3919633/1‑101 (MQ=255)
                               ccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGcc                                                                       <  1:2494557/101‑1 (MQ=255)
                               ccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGcc                                                                       <  2:3818818/101‑1 (MQ=255)
                                     tCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTcc                                                                 >  2:339684/1‑101 (MQ=255)
                                      cTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTGGATCG.TGCTCAGCCCGGCCATGTCCt                                                                >  1:1683923/1‑101 (MQ=255)
                                        gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                                                              >  2:2718780/1‑101 (MQ=255)
                                        gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                                                              <  2:2409724/101‑1 (MQ=255)
                                            cgTGCCCAGCACCCCCAGCTGGGCCGTGCGCAGCGCCCCCTTCTGCCCCCCGGTCCGCGCCGCCAGTTCGATCG.TGCTCCGCCCGGCCATGTCCTCGGTCg                                                          <  1:1277558/101‑1 (MQ=255)
                                            cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                                                          <  1:1872699/101‑1 (MQ=255)
                                            cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                                                          >  1:1177330/1‑101 (MQ=255)
                                             gTGCCCAGAACCCCCAGATGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGa                                                         >  1:3993480/1‑101 (MQ=255)
                                              tGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaa                                                        >  1:4073611/1‑101 (MQ=255)
                                               gCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaaa                                                       >  2:167300/1‑101 (MQ=255)
                                               gCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaaa                                                       >  2:3872567/1‑101 (MQ=255)
                                                cccAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGTTCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAg                                                      <  1:1039325/101‑1 (MQ=255)
                                                 gcccaccccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGc                                                     <  1:1894203/95‑1 (MQ=255)
                                                  cAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcc                                                    >  1:41459/1‑101 (MQ=255)
                                                  cAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcc                                                    <  2:3369387/101‑1 (MQ=255)
                                                   aGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGccc                                                   >  1:1305689/1‑101 (MQ=255)
                                                   aGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGccc                                                   <  1:2718986/101‑1 (MQ=255)
                                                     cgccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCt                                                 >  1:1014608/6‑101 (MQ=37)
                                                     cgccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCt                                                 <  2:1111772/96‑1 (MQ=38)
                                                     aaCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCt                                                 <  1:4204687/101‑1 (MQ=255)
                                                      gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt                                                <  2:3613280/97‑1 (MQ=37)
                                                      aCCCCCAGCTGGGCCGTTCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCtt                                                <  2:4183319/101‑1 (MQ=255)
                                                      aCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCtt                                                <  1:1070331/101‑1 (MQ=255)
                                                      aCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCtt                                                <  1:4025104/101‑1 (MQ=255)
                                                       cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc                                               <  1:2451348/101‑1 (MQ=255)
                                                        cgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttga                                              <  1:3127621/99‑3 (MQ=37)
                                                        ccccGGCGGGGCCGTGCTCAGCCCCGCCATCTGCGCCCCGGTCACCGCAGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa                                              <  2:2791736/101‑1 (MQ=255)
                                                        ccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa                                              <  1:792981/101‑1 (MQ=255)
                                                         gccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAg                                             >  1:2267338/2‑101 (MQ=37)
                                                         gccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAg                                             <  2:545324/100‑1 (MQ=25)
                                                         gccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAg                                             <  1:1610884/100‑1 (MQ=25)
                                                         cccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAg                                             <  1:1379032/101‑1 (MQ=255)
                                                         cccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAg                                             >  1:820830/1‑101 (MQ=255)
                                                          ccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgc                                            >  1:2916034/1‑101 (MQ=255)
                                                          ccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgc                                            <  2:820749/101‑1 (MQ=255)
                                                          ccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgc                                            <  1:2231862/101‑1 (MQ=25)
                                                           cAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcg                                           <  2:1483455/101‑1 (MQ=255)
                                                           cAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgcg                                           >  1:3064618/1‑101 (MQ=25)
                                                             gCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGcgcg                                         >  1:3217849/1‑101 (MQ=255)
                                                              cTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcgg                                        >  2:733715/1‑101 (MQ=255)
                                                              cTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcgg                                        >  2:3209454/1‑101 (MQ=255)
                                                               tgcgCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgcgggcc                                       >  2:2101379/4‑97 (MQ=25)
                                                               tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTCCAGCgcggc                                       >  1:984641/1‑101 (MQ=255)
                                                                gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg                                      <  2:1510979/101‑1 (MQ=255)
                                                                   ccGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTg                                   <  1:357660/101‑1 (MQ=255)
                                                                     gTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGcc                                 <  2:3727123/101‑1 (MQ=255)
                                                                      tGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCg                                >  2:3425015/1‑101 (MQ=255)
                                                                        cTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGAt                              >  1:739152/1‑101 (MQ=255)
                                                                         tCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATc                             <  2:3030214/101‑1 (MQ=255)
                                                                         tCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATc                             >  1:2634751/1‑101 (MQ=255)
                                                                         tCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATc                             <  1:339735/101‑1 (MQ=255)
                                                                         tCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATc                             >  2:3927351/1‑101 (MQ=255)
                                                                           aGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgc                           >  2:585448/1‑101 (MQ=255)
                                                                             cgccgcCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccg                         <  1:214804/101‑1 (MQ=255)
                                                                             cgccgcCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccg                         >  2:2072408/1‑101 (MQ=255)
                                                                              gccgccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccgc                        <  2:2519403/101‑1 (MQ=255)
                                                                                 gccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaaa                     <  2:1021108/101‑1 (MQ=255)
                                                                                    aTCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGc                  >  2:896352/1‑101 (MQ=255)
                                                                                     tCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGcc                 >  1:1932161/1‑101 (MQ=255)
                                                                                       tGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCcgc               >  2:1968471/1‑101 (MQ=255)
                                                                                           ccccGGTCAGCGCCTCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCg           <  2:2827374/101‑1 (MQ=255)
                                                                                           ccccGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCg           <  1:2839460/101‑1 (MQ=255)
                                                                                           ccccGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCg           <  2:1305593/101‑1 (MQ=255)
                                                                                            cccGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCgg          <  1:54916/101‑1 (MQ=255)
                                                                                             ccGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGa         <  2:2585695/101‑1 (MQ=255)
                                                                                              cGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaa        >  2:2327835/1‑101 (MQ=255)
                                                                                              cGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaa        >  2:2023486/1‑101 (MQ=255)
                                                                                               ggTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaaa       >  1:3441391/1‑101 (MQ=255)
                                                                                                gTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaaaa      >  1:3272862/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCG  >  NC_014034/682456‑682656

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG