Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 698,491 T→G 8.6% T533P (ACC→CCC)  RCAP_rcc00640 ← S1/S6 family peptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034698,4910T→G 8.6% 2.8 ‑1.6 0.082T533P (ACC→CCC) RCAP_rcc00640S1/S6 family peptidase
Reads supporting (aligned to +/- strand):  new base (11/0):  ref base (58/13):  total (69/13)
Fisher's exact test strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GGGGGCGGGGGCCGGGGCGGGGGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAACCCGGCCG  >  NC_014034/698393‑698591
                                                                                                  |                                                                                                    
gggggcgggggcCGGGGCGGGGGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTc                                                                                                    >  1:1063170/1‑101 (MQ=255)
      gggggcCGGGGCGGGGGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCCGGGGGTCCGGGCa                                                                                              >  2:3109276/1‑101 (MQ=255)
       ggggcCGGGGCGGGGGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGGCCGGCGTCACGGCGGCGGGGGTTCCGGCCAg                                                                                             >  1:647965/1‑101 (MQ=255)
         ggcCGGGGCGGGGGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGcccgca                                                                                           >  2:174462/1‑97 (MQ=255)
          gcCGGGGCGGGGGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGaaa                                                                                          >  2:3181967/1‑101 (MQ=255)
            cggggcggggGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGgaaat                                                                                        >  1:4199032/1‑99 (MQ=255)
             ggggcggggGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAAATc                                                                                       >  2:2943910/1‑101 (MQ=255)
                 cggggGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGcc                                                                                   >  2:3709280/1‑101 (MQ=255)
                   ggggTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCCGCCAGAAACCCGGCCAg                                                                                 >  2:1281189/1‑101 (MQ=255)
                    gggTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAAATCgggccga                                                                                >  1:824007/1‑96 (MQ=255)
                      gTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTTCGGCCCGGAACACGGGCCGAcc                                                                              >  1:347250/1‑101 (MQ=38)
                        cGGGGCGGGCGTCGCCGAAGCGGGCGCCACAGTCGGGTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCCGGGGTTCCTGCCGGAAACTCGGCCCGACCcg                                                                            >  1:3766630/1‑101 (MQ=255)
                        cGGGGCGGGCGTCGCCGAAGCAGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCGAGACCcg                                                                            >  1:2409730/1‑101 (MQ=255)
                         ggggCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCCCGACCcgc                                                                           >  1:3860193/1‑101 (MQ=255)
                          gggcgggcGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCcgcg                                                                          >  1:2833677/1‑101 (MQ=255)
                          gggcgggcGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCcgcg                                                                          >  1:1351442/1‑101 (MQ=255)
                            gcgggcgTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCACACCCCCGGc                                                                        >  2:1998251/1‑101 (MQ=255)
                            gcgggcgTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCCAGACCCGCGGc                                                                        >  1:1924259/1‑101 (MQ=255)
                             cgggcgTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCCAGACCCGCGGcc                                                                       >  1:4012293/1‑101 (MQ=255)
                              gggcgTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCg                                                                      >  1:1722387/1‑101 (MQ=255)
                              gggcgTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCg                                                                      >  1:552093/1‑101 (MQ=255)
                               ggcgTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGa                                                                     >  1:2835101/1‑101 (MQ=255)
                               ggcgTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGa                                                                     >  1:1373841/1‑101 (MQ=255)
                                 cgTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGaaa                                                                   <  1:3816512/101‑1 (MQ=255)
                                 cgTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGaaa                                                                   >  1:2510039/1‑101 (MQ=255)
                                  gTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGGCAGACCCGCCGCCGAAAg                                                                  >  2:3258583/1‑101 (MQ=255)
                                  gTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAg                                                                  >  1:1382838/1‑101 (MQ=255)
                                  gTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTACGGCCAGAAACTCGGCCAGAACCGCGGGCGAAAg                                                                  >  1:2976129/1‑101 (MQ=255)
                                    cGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGGCGAAAgcg                                                                >  2:382387/1‑101 (MQ=255)
                                     gCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGGCGGAAgcgc                                                               >  2:2283170/1‑101 (MQ=255)
                                     gCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAgcgc                                                               >  1:3407172/1‑101 (MQ=255)
                                      ccGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGcgcg                                                              >  1:1185318/1‑101 (MQ=255)
                                       cGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGa                                                             >  2:4068769/1‑101 (MQ=255)
                                        gAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGGGGCCGaaacccggaa                                                            >  1:1604247/1‑94 (MQ=255)
                                           gCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAAtt                                                         <  1:2283338/101‑1 (MQ=255)
                                            cGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTc                                                        >  1:1577807/1‑101 (MQ=255)
                                            cGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTc                                                        >  1:2430815/1‑101 (MQ=255)
                                             gggCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCa                                                       <  1:1281285/101‑1 (MQ=255)
                                             gggCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCCAGACCCGCGGGCGAAAGCCCGAAATTCa                                                       >  1:2050129/1‑101 (MQ=255)
                                               gcgcCCCCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGc                                                     <  1:3109507/101‑1 (MQ=255)
                                               gcgcCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGc                                                     >  1:3905905/1‑101 (MQ=255)
                                                cgcCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCCAGACCCGCGGCCCAAAGCGCGGAATTTAGCg                                                    >  1:3335931/1‑101 (MQ=255)
                                                   cACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTgc                                                 >  1:779601/1‑101 (MQ=255)
                                                    aCCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGGGCGAAATTCAGCGTgcg                                                >  1:2670061/1‑101 (MQ=255)
                                                      cGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTgcgcc                                              >  1:3468676/1‑100 (MQ=255)
                                                      cGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTgcgcc                                              >  1:2741825/1‑100 (MQ=255)
                                                      cGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGGGCGCa                                              >  1:764031/1‑101 (MQ=255)
                                                       gTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTTCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGGGAAACTCCGCGTGCGCAg                                             >  2:2678830/1‑101 (MQ=255)
                                                       gTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTgcgccg                                             >  1:3116547/1‑99 (MQ=255)
                                                       gTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAg                                             >  1:948051/1‑101 (MQ=255)
                                                        tCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGACAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGc                                            >  2:1619392/1‑101 (MQ=255)
                                                         cGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCg                                           >  1:3980700/1‑101 (MQ=255)
                                                          ggTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGACAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCgg                                          <  1:1619501/101‑1 (MQ=255)
                                                           gTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCggg                                         >  2:2554465/1‑101 (MQ=255)
                                                           gTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCggg                                         >  1:1184854/1‑101 (MQ=255)
                                                             tGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGGGAAATTCAGCGTGGGCAGCGGGcc                                       >  2:3117992/1‑101 (MQ=255)
                                                             tGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGcc                                       <  2:347199/101‑1 (MQ=255)
                                                               gCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCtt                                     <  2:1382738/101‑1 (MQ=255)
                                                                  gCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGcc                                  >  2:1131402/1‑101 (MQ=255)
                                                                    tGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCgg                                >  2:441478/1‑101 (MQ=255)
                                                                    tGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCgg                                >  1:2097700/1‑101 (MQ=255)
                                                                     ggCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGa                               >  1:3336595/1‑101 (MQ=255)
                                                                     ggCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACCCGGCCAGACCCGCGGCCGCAAGCGCGAAATTCAGCGTGGGCAGCGGGCCCCGCCggc                               >  2:592045/1‑100 (MQ=255)
                                                                      gcgcGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGAc                              >  2:444536/1‑101 (MQ=255)
                                                                       cgcgGTCCGGCGTCCCGGCGGCGGGGGTTGCGGCCAGAAACTCGGGCCGACCCGAGGCCGGAAGAGCGAAACTCAGCGTTGGCAGTGGGTCTTGCCggcgg                             >  2:3549543/1‑98 (MQ=255)
                                                                        gcgGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGa                            >  1:268570/1‑101 (MQ=255)
                                                                        gcgGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCATTGCCGGACGa                            >  2:254725/1‑101 (MQ=255)
                                                                         cgGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGaa                           >  2:1188900/1‑101 (MQ=255)
                                                                         cgGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGaa                           >  2:3101930/1‑101 (MQ=255)
                                                                            tCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGGCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAggg                        >  1:2470511/1‑100 (MQ=255)
                                                                               ggtgTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGtt                     >  1:3097157/4‑101 (MQ=255)
                                                                               ggCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGGGtt                     >  1:3184808/1‑101 (MQ=255)
                                                                               ggCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGACAGACCCGCGGGCGAAAGCGCGAAATCCAGCGAGCGCAGCGGGCCTTGCCGGACGAAGgggta                     >  1:2074881/1‑100 (MQ=255)
                                                                                  ggcACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCAc                  <  2:2049991/99‑1 (MQ=255)
                                                                                  gTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGGTCAc                  >  2:1542325/1‑101 (MQ=255)
                                                                                  gTCACGGCGGCGGGGGGTCCGGCCAGAAACTCGGCCAGACCCCCGGCCGGAAGCGCGAAAATCAGCGTGCGCAGCGGGGCCTGCCGGGCGCAGGGGGTCAc                  >  1:1079431/1‑101 (MQ=255)
                                                                                     aCGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCAcccc               <  2:1185224/101‑1 (MQ=255)
                                                                                     aCGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCAcccc               >  2:2104255/1‑101 (MQ=255)
                                                                                     aCGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGGGTTCAcccc               >  1:3665216/1‑101 (MQ=255)
                                                                                     aCGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGGGTTCAcccc               >  1:1281012/1‑101 (MQ=255)
                                                                                     aCGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGGGTTCAcccc               >  2:1257483/1‑101 (MQ=255)
                                                                                     aCGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCCTGCGCAGCGGGCCTTGCCCGACGAAGGGGGTCAcccc               >  2:2919871/1‑101 (MQ=255)
                                                                                       ggcggcggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCga             >  1:3396384/1‑101 (MQ=255)
                                                                                         cggcggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCgaga           >  2:2579654/1‑101 (MQ=255)
                                                                                         cggcggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCgaga           <  2:2509855/101‑1 (MQ=255)
                                                                                          ggcggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGaa          >  2:2379896/1‑101 (MQ=255)
                                                                                          ggcggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGaa          >  2:1136051/1‑101 (MQ=255)
                                                                                          ggcggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGaa          <  2:1924133/101‑1 (MQ=255)
                                                                                          ggcggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGaa          >  2:78388/1‑101 (MQ=255)
                                                                                          ggcggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGaa          <  2:947966/101‑1 (MQ=255)
                                                                                           gcggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAAc         >  1:694704/1‑101 (MQ=255)
                                                                                            cggGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAAcc        >  1:1308499/1‑101 (MQ=255)
                                                                                             gggggTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAAccc       <  2:3406927/101‑1 (MQ=255)
                                                                                             gggggTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAAccc       >  2:781921/1‑101 (MQ=255)
                                                                                             gggggTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGGGTTCACCCCGAGAAccc       >  2:2011516/1‑101 (MQ=255)
                                                                                             gggggTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGCGTTCACCCCGAGAAccc       >  1:21849/1‑101 (MQ=255)
                                                                                             gggggTTCCGACCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAAccc       >  1:1419412/1‑101 (MQ=255)
                                                                                              ggggTTCCGGCCAGAAACTCGGCCATACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAACCCg      >  1:3905933/1‑101 (MQ=255)
                                                                                              ggggTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCTGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAAACCg      >  2:3884187/1‑101 (MQ=255)
                                                                                                  ttCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAACCCGGCCg  >  1:1553464/1‑101 (MQ=255)
                                                                                                  |                                                                                                    
GGGGGCGGGGGCCGGGGCGGGGGTCGGGGCGGGCGTCGCCGAAGCGGGCGCCACCGTCGGTTGGCAGCTGGCGCGGTCCGGCGTCACGGCGGCGGGGGTTCCGGCCAGAAACTCGGCCAGACCCGCGGCCGAAAGCGCGAAATTCAGCGTGCGCAGCGGGCCTTGCCGGACGAAGGTGTTCACCCCGAGAACCCGGCCG  >  NC_014034/698393‑698591

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG