Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	974,361	C→G	100%	intergenic (+241/+18)	RCAP_rcc00884 → / ← RCAP_rcc00885	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	974,361	0	C→G	100.0%	166.8	46	intergenic (+241/+18)	RCAP_rcc00884/RCAP_rcc00885	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (32/13):  ref base (0/0):  total (32/14)

CACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGCCC  >  NC_014034/974284‑974458                                                                                  |                                                                                                  cACCTTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGGGGCGCGGCGGCAACCGCGGCGGGAAACACCCGCCGCAACGGCCggggctc                                                                                >  1:231320/1‑96 (MQ=0)                                  ggggccccccGGCGGGGCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCa                                               <  2:204132/96‑1 (MQ=255)                                  gggccccccgGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCa                                               <  1:2468777/96‑1 (MQ=255)                                     ccccccgGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCAccc                                            <  1:2431043/99‑1 (MQ=255)                                       ctgcgggcggTTCGCCGGGGCGACGGCGGGGGGAAAACCCCCCGGCCCCAACGGCCGTTTCTCAGCCAAACAGGATAAGGTTCGAGCGCGCGATCCCCCag                                          <  2:3956167/96‑1 (MQ=255)                                             gcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtc                                    <  2:2464748/101‑1 (MQ=255)                                               gggTCGCCTCGGGGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcat                                  <  1:1667921/101‑1 (MQ=255)                                               gggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcat                                  <  1:3534556/101‑1 (MQ=255)                                               gggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcat                                  <  1:189733/101‑1 (MQ=255)                                               gggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcat                                  >  1:3961471/1‑101 (MQ=255)                                                ggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                                 <  1:2897875/101‑1 (MQ=255)                                                gatCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                                 >  1:1005182/3‑101 (MQ=255)                                                 gTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatcc                                >  1:1508029/1‑100 (MQ=255)                                                  tCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGTATCAGGTTCGAGTGCGCGATCACCCAGAGGGtcatctg                               >  1:983328/1‑99 (MQ=255)                                                  tCGCCTCGGCGACAGCCGAGGCGAAATACCCCAGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAg                               <  1:3735437/101‑1 (MQ=255)                                                   cGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGGCATCAgc                              >  1:593242/1‑101 (MQ=255)                                                    gCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgcg                             <  1:549966/101‑1 (MQ=255)                                                      cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                           >  1:3882702/1‑101 (MQ=255)                                                        cGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                         <  1:874421/101‑1 (MQ=255)                                                         ggCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAg                        >  2:747115/1‑101 (MQ=255)                                                         ggCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAg                        >  1:4044855/1‑101 (MQ=255)                                                          gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       <  2:72378/101‑1 (MQ=255)                                                           cGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGc                      >  1:1416413/1‑101 (MQ=255)                                                            gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     <  2:3706087/101‑1 (MQ=255)                                                              cAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGc                   <  1:3775344/101‑1 (MQ=255)                                                              cAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGc                   >  2:2083014/1‑101 (MQ=255)                                                              cAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGc                   >  1:574763/1‑101 (MQ=255)                                                               aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                  >  1:3120478/1‑101 (MQ=255)                                                                gCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaa                 >  2:733521/1‑101 (MQ=255)                                                                 ccGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGTTCACCCAGAGGGTCATCAGCGCATAGGCCGCaaa                >  2:236700/1‑101 (MQ=255)                                                                  cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCCAAc               >  2:1860938/1‑101 (MQ=255)                                                                  cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAc               >  2:4014089/1‑101 (MQ=255)                                                                   gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc              >  2:283520/1‑101 (MQ=255)                                                                   gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc              >  1:151360/1‑101 (MQ=255)                                                                   gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc              >  2:1886719/1‑101 (MQ=255)                                                                     ggCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcccc            >  1:2770301/1‑101 (MQ=255)                                                                     ggCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcccc            >  2:2505498/1‑101 (MQ=255)                                                                       cGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGc          >  1:125026/1‑101 (MQ=255)                                                                        gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         >  1:55383/1‑101 (MQ=255)                                                                        gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         >  1:2383065/1‑101 (MQ=255)                                                                         aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa        >  1:185685/1‑101 (MQ=255)                                                                         aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa        >  1:1874505/1‑101 (MQ=255)                                                                          aaTCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAg       <  2:3136513/101‑1 (MQ=255)                                                                          aaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAg       >  2:3032087/1‑101 (MQ=255)                                                                          aaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAg       >  2:4056494/1‑101 (MQ=255)                                                                           aTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgc      >  2:3561029/1‑101 (MQ=255)                                                                           aTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgc      >  1:2174605/1‑101 (MQ=255)                                                                           aTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATAACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgc      >  1:313379/1‑101 (MQ=255)                                                                            tACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcg     >  1:1556104/1‑101 (MQ=255)                                                                             cccccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcgc    <  2:2900986/100‑1 (MQ=255)                                                                              ccccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGcc   >  2:3183618/1‑101 (MQ=255)                                                                               cccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGccc  >  1:519427/1‑101 (MQ=255)                                                                                  |                                                                                                  CACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGCCC  >  NC_014034/974284‑974458 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG