Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,732,707	3 bp→TCC	100%	intergenic (‑131/‑270)	RCAP_rcc01601 ← / → tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,732,707	0	G→T	100.0%	87.2	34	intergenic (‑131/‑272)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/34):  ref base (0/0):  total (0/34)
*	NC_014034	1,732,708	0	G→C	100.0%	86.8	34	intergenic (‑132/‑271)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/34):  ref base (0/0):  total (0/34)
*	NC_014034	1,732,709	0	G→C	100.0%	87.1	34	intergenic (‑133/‑270)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/34):  ref base (0/0):  total (0/34)

AGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.A.CC..GGGC..GGGCGG..G..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATG  >  NC_014034/1732609‑1732778                                                                                                          |||||                                                                        aGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGGTTCGCCGCGGCGGGGGCCCGGGGG.C.Cc..gggc..gggggg                                                                            >  1:1168327/1‑101 (MQ=255)  gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.A.CC..CGGG..GGGGgg..g..g                                                                      >  2:2690075/1‑101 (MQ=255)      aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..CGGC..GGGGgg..g..gggg                                                                   >  1:2256525/1‑98 (MQ=255)       tCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.A.CCCGGGGC..GGGGgg..g..ggcg                                                                   >  2:1792202/1‑98 (MQ=255)          gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGGCGCGGCC.A.CC..CGGG..GGGGgg..g..ggggggccc                                                              >  1:2766531/1‑93 (MQ=255)           ggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGGCGCGGCG.A.CC..CGGG..GGGGGG..G..Tccccccccc                                                             >  1:4151882/1‑97 (MQ=255)              gagaGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.A.CC..CCGG..GGGGGG..G..GGCCCCCCCcccc                                                          >  1:970843/1‑101 (MQ=255)                 agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAcccc                                                          <  2:4151620/101‑1 (MQ=25)                  ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAccccc                                                         <  2:3159941/101‑1 (MQ=25)                  ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAccccc                                                         <  1:3287269/101‑1 (MQ=25)                  ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAccccc                                                         <  2:1678316/101‑1 (MQ=25)                  ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCAccccc                                                         <  2:1609783/101‑1 (MQ=25)                    tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCgg                                                       <  2:2756478/101‑1 (MQ=25)                    tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCgg                                                       <  1:3278563/101‑1 (MQ=25)                    tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGC..GGGGGG..G..CCGCCCCCCccccccccc                                                     >  1:414817/1‑97 (MQ=255)                     aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGC..GGGGGG..G..GCGCCCCCCcccccccccg                                                    >  2:2528008/1‑96 (MQ=255)                           cGTCGGTGCGGGGCGGACGGCCGGGTGCCAAGCAGGGTTTCGCCGCGGG.GGGGCCGCGGCGCC.CC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTAttt                                                <  2:1530854/101‑1 (MQ=16)                            gTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTg                                               <  1:1894797/101‑1 (MQ=21)                             tCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTgc                                              <  1:1054370/101‑1 (MQ=21)                             tCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGC..GGGGGG..GCCGCCCCCGCCCCCCCCGCggcctttg                                              >  2:1030967/1‑94 (MQ=255)                                 tGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCa                                          <  2:3485951/101‑1 (MQ=21)                                    ggggCGGAAAGCCGCGTGCCAGGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGTGCCaaga                                       <  2:1265441/101‑1 (MQ=18)                                    ggggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTCTTTGCGCCaaga                                       <  1:2298605/101‑1 (MQ=21)                                    ggggCAGAAGGCCGCGTGCCCAGAATGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGT..GGGGTGTCC..GCCCCCCCACCTCCGGCTTATTTGCGTCaaga                                       <  2:1080930/101‑1 (MQ=255)                                     gggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaagaa                                      <  2:941662/101‑1 (MQ=21)                                      gcgagAAAGCCGCGTGCCAGGCGGGGGTGCGCGGCGGG.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaagaag                                     <  2:1469560/97‑1 (MQ=9)                                        cGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAgaagaa                                   <  1:3872664/101‑1 (MQ=18)                                         ggAGAGCCGCGTGCCAAGCGGGGTTTCGCCGGGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                  <  1:2921000/101‑1 (MQ=17)                                         ggAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                  <  2:3776171/101‑1 (MQ=18)                                         ggAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                  <  2:759145/101‑1 (MQ=18)                                          gAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAAc                                 <  1:2126933/101‑1 (MQ=18)                                          gAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAAc                                 <  2:163316/101‑1 (MQ=18)                                               ccGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCg                            <  2:1785524/101‑1 (MQ=18)                                                  cgTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGa                         <  2:3437024/101‑1 (MQ=18)                                                   gTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAc                        <  2:2892352/101‑1 (MQ=18)                                                    tGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAcc                       <  1:1954841/101‑1 (MQ=18)                                                      ccAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                     <  1:2690281/101‑1 (MQ=18)                                                       cAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAgg                    <  2:2133135/101‑1 (MQ=18)                                                        aaGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGc                   <  2:2256366/101‑1 (MQ=18)                                                         aGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcc                  <  1:3210292/101‑1 (MQ=18)                                                             gggTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCTGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccccc              <  2:3061714/101‑1 (MQ=18)                                                               gTTTCGCCGCGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTg            <  1:3254114/101‑1 (MQ=18)                                                                   cgccgcGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGc        <  2:2680733/101‑1 (MQ=18)                                                                     ccgcGGC.GGGGCCGCGGCGTG.CC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCg      <  1:2523589/101‑1 (MQ=21)                                                                     ccgcGGC.GGGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCg      <  1:428965/101‑1 (MQ=18)                                                                        cggc.ggGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCAt   <  2:2857051/101‑1 (MQ=18)                                                                         ggc.ggGGCCGCGGCG.ACCC..GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATg  <  1:2528192/101‑1 (MQ=18)                                                                                                          |||||                                                                        AGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGC.GGGGCCGCGGCG.A.CC..GGGC..GGGCGG..G..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATG  >  NC_014034/1732609‑1732778 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG