Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,802,243 2 bp→GC 100% coding (626‑627/2124 nt) ligA → DNA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,802,2430C→G100.0% 582.1 162T209R (ACG→AGG) ligADNA ligase
Reads supporting (aligned to +/- strand):  new base (82/80):  ref base (0/0):  total (82/80)
*NC_0140341,802,2440G→C100.0% 601.7 162T209T (ACG→ACCligADNA ligase
Reads supporting (aligned to +/- strand):  new base (82/80):  ref base (0/0):  total (82/80)

Only 100 of 164 total aligned reads displayed.

GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338
                                                                                               ||                                                                                              
  cgcCTGCCGTGCTGGAGGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGcc                                                                                          <  1:2871614/101‑1 (MQ=255)
  cgcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGcc                                                                                          >  1:2254590/1‑101 (MQ=255)
   gcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCa                                                                                         >  1:2609955/1‑101 (MQ=255)
   gcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCa                                                                                         <  1:3212847/101‑1 (MQ=255)
   gcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCa                                                                                         >  1:3693682/1‑101 (MQ=255)
    ccTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCaa                                                                                        >  2:394486/1‑101 (MQ=255)
     cTGCCGTGCTGGAAGTCCGCGGGGAGGTGTGCATGAGCCATGGCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAAt                                                                                       <  2:1690317/101‑1 (MQ=255)
     cTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAAt                                                                                       <  1:2382471/101‑1 (MQ=255)
     cTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAAt                                                                                       >  2:2543875/1‑101 (MQ=255)
      tGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATc                                                                                      <  2:3102125/101‑1 (MQ=255)
        ccGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcg                                                                                    >  1:442767/1‑101 (MQ=255)
           tGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCgcgc                                                                                 >  2:766076/1‑101 (MQ=255)
            gCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCa                                                                                >  2:1006247/1‑101 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              <  1:1448972/101‑1 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              >  2:3706993/1‑101 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              <  1:2544066/101‑1 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              <  1:3247607/101‑1 (MQ=255)
                gAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCCATgc                                                                            >  1:1032340/1‑101 (MQ=255)
                   gTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccgc                                                                         >  2:2621957/1‑101 (MQ=255)
                   gTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccgc                                                                         >  1:4232771/1‑101 (MQ=255)
                    tCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccgcc                                                                        >  1:213111/1‑101 (MQ=255)
                    tCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccgcc                                                                        >  2:2882541/1‑101 (MQ=255)
                       gcggcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCcgcg                                                                     <  1:2028411/101‑1 (MQ=255)
                        cggcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCgg                                                                    >  1:1833090/1‑101 (MQ=255)
                           cgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCt                                                                 >  1:2933955/1‑101 (MQ=255)
                            gAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTc                                                                >  1:2261388/1‑101 (MQ=255)
                            gAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTc                                                                >  2:865894/1‑101 (MQ=255)
                            gAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATACGCGCAATGCCGCCGCGGGCTc                                                                >  2:209951/1‑101 (MQ=255)
                               gTCTGCATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGCGCTTCGCCAATCCGCGCCATGCcgccgccgccccgcc                                                             >  2:858253/1‑92 (MQ=255)
                               gTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgat                                                             <  2:921880/101‑4 (MQ=255)
                                tCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctg                                                            >  2:792912/1‑97 (MQ=255)
                                 cTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctgc                                                           >  1:2675228/1‑96 (MQ=255)
                                 cTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctgc                                                           >  1:625462/1‑96 (MQ=255)
                                 cTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctgc                                                           >  1:3364189/1‑96 (MQ=255)
                                  tACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcg                                                          >  2:2128328/1‑101 (MQ=255)
                                  tACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcg                                                          >  2:1582321/1‑101 (MQ=255)
                                   aCATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcgc                                                         >  1:2202311/1‑101 (MQ=255)
                                    cATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                        <  2:213073/101‑1 (MQ=255)
                                      tGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAg                                                      <  2:500337/101‑1 (MQ=255)
                                      tGAGCCATGACGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAg                                                      <  2:460421/101‑1 (MQ=255)
                                        aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCt                                                    >  1:2524050/1‑101 (MQ=255)
                                          ccATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCg                                                  <  1:3311368/101‑1 (MQ=255)
                                          ccATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCg                                                  >  1:4121095/1‑101 (MQ=255)
                                          ccATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCg                                                  <  1:3043127/101‑1 (MQ=255)
                                           cATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCgc                                                 >  2:735015/1‑100 (MQ=255)
                                           cATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGa                                                 >  2:1621621/1‑101 (MQ=255)
                                           cATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGa                                                 >  1:4005499/1‑101 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                <  1:1006338/101‑1 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                <  1:2440967/101‑1 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                >  1:3887644/1‑101 (MQ=255)
                                             tGCCGATTTTTCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACg                                               >  2:1771755/1‑101 (MQ=255)
                                                     ttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCACCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATc                                       <  2:1868492/101‑1 (MQ=255)
                                                      tGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCa                                      <  2:1656382/101‑1 (MQ=255)
                                                       gcggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAc                                     >  2:2451748/1‑101 (MQ=255)
                                                        cggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCTCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAcc                                    <  2:1970184/101‑1 (MQ=255)
                                                        cggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAcc                                    <  2:3006463/101‑1 (MQ=255)
                                                          gcgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGc                                  >  2:1038701/1‑101 (MQ=255)
                                                            gcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCt                                >  2:1746517/1‑101 (MQ=255)
                                                            gcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCt                                >  1:860494/1‑101 (MQ=255)
                                                             cTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTc                               >  2:235984/1‑101 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCTTcc                              >  2:2080578/1‑101 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              >  2:1185916/1‑101 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              <  2:483369/101‑1 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             >  1:3324946/1‑101 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            <  1:2128473/101‑1 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            <  1:2039031/101‑1 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            <  2:1042513/101‑1 (MQ=255)
                                                                 aTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGa                           >  1:3531397/1‑101 (MQ=255)
                                                                  tGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAc                          <  1:1186010/101‑1 (MQ=255)
                                                                     gcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCt                       <  1:1384995/101‑1 (MQ=255)
                                                                     gcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCt                       <  1:792990/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  2:4032750/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  2:625398/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  1:1078633/101‑1 (MQ=255)
                                                                        gCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGa                    <  1:656906/101‑1 (MQ=255)
                                                                        gCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGa                    <  1:2937772/101‑1 (MQ=255)
                                                                         cAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaa                   <  1:309793/101‑1 (MQ=255)
                                                                          aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                  <  1:808204/101‑1 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 >  1:1558549/1‑101 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 <  1:394537/101‑1 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 <  1:3123517/101‑1 (MQ=255)
                                                                            gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAtt                <  1:3198502/101‑1 (MQ=255)
                                                                            gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAtt                <  1:766153/101‑1 (MQ=255)
                                                                             cGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTc               >  1:3976299/1‑101 (MQ=255)
                                                                                   ggggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGcc         >  2:2381234/1‑101 (MQ=255)
                                                                                   ggggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGcc         <  2:3404262/101‑1 (MQ=255)
                                                                                    gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt        >  2:2754386/1‑101 (MQ=255)
                                                                                     ggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAAACCCTTGAAATTCTTTGCCTa       >  1:1629305/1‑101 (MQ=255)
                                                                                      gCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAt      >  2:2587549/1‑101 (MQ=255)
                                                                                      gCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAt      <  2:1633245/101‑1 (MQ=255)
                                                                                       cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     <  1:122680/101‑1 (MQ=255)
                                                                                        gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTATGCCTATTc    <  2:3324705/101‑1 (MQ=255)
                                                                                         aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc   >  2:3501661/1‑101 (MQ=255)
                                                                                         aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc   <  1:1874995/101‑1 (MQ=255)
                                                                                         aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc   <  2:3051045/101‑1 (MQ=255)
                                                                                         aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc   >  1:840144/1‑101 (MQ=255)
                                                                                         aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc   <  1:3707247/101‑1 (MQ=255)
                                                                                          cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt  <  2:370800/101‑1 (MQ=255)
                                                                                          cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt  <  1:3510845/101‑1 (MQ=255)
                                                                                          cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt  <  2:2592085/101‑1 (MQ=255)
                                                                                               ||                                                                                              
GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG