Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,992,043 2 bp→GC 100% coding (582‑583/2169 nt) glgB ← 1,4‑alpha‑glucan branching protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,992,0430A→G100.0% 542.7 140F195L (TTC→CTC) glgB1,4‑alpha‑glucan branching protein
Reads supporting (aligned to +/- strand):  new base (68/72):  ref base (0/0):  total (68/72)
*NC_0140341,992,0440A→C100.0% 542.0 140P194P (CCT→CCGglgB1,4‑alpha‑glucan branching protein
Reads supporting (aligned to +/- strand):  new base (67/73):  ref base (0/0):  total (67/73)

Only 100 of 172 total aligned reads displayed.

GGCGCCGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAAAGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAACCCCCGTCGCGC  >  NC_014034/1991948‑1992138
                                                                                               ||                                                                                              
ggCGCCGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGGCGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCt                                                                                            <  2:1984570/101‑1 (MQ=255)
ggCGCCGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCt                                                                                            >  1:1894034/1‑101 (MQ=255)
ggCGCCGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCt                                                                                            <  1:549270/101‑1 (MQ=255)
 gcgcCGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTg                                                                                           >  1:3807357/1‑101 (MQ=255)
  cgcCGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGa                                                                                          >  1:4020317/1‑101 (MQ=255)
   gcCGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGaa                                                                                         <  2:620591/101‑1 (MQ=255)
     cGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAacg                                                                                       <  1:2374373/101‑1 (MQ=255)
     cGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAacg                                                                                       <  2:3485336/101‑1 (MQ=255)
      gTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAacga                                                                                      >  2:1814819/1‑101 (MQ=255)
       tCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGCCGCTCCGCGCCAAACCCCACCCGATCGGCCTTGCGCGGCCGAacgcc                                                                                     >  2:3420801/1‑99 (MQ=255)
       tCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAacgac                                                                                     <  1:2672248/101‑1 (MQ=255)
        cGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAacgacg                                                                                    >  1:2593179/1‑101 (MQ=255)
         gCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGc                                                                                   >  2:2042349/1‑101 (MQ=255)
         gCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGc                                                                                   >  2:3753790/1‑101 (MQ=255)
          ccccACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAAACCCACCGGATCGGCCTTGAGCGGCTGAACGACGcc                                                                                  <  2:4010104/101‑1 (MQ=255)
           cccACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCa                                                                                 >  2:2742907/1‑101 (MQ=255)
             cacaGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATc                                                                               <  1:62408/101‑1 (MQ=255)
             cacaGATGCAGTCCAAGCTCGCGGACCTGAGACACCGACTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATc                                                                               <  1:3621065/101‑1 (MQ=255)
              acaGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCg                                                                              <  1:4034461/101‑1 (MQ=255)
               caGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCgg                                                                             >  2:797759/1‑101 (MQ=255)
                aGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGc                                                                            <  2:3649207/101‑1 (MQ=255)
                 gATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGcc                                                                           <  2:737521/101‑1 (MQ=255)
                  aTGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCg                                                                          >  2:316540/1‑101 (MQ=255)
                  aTGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCg                                                                          <  1:1341967/101‑1 (MQ=255)
                   tGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGa                                                                         <  2:1785708/101‑1 (MQ=255)
                   tGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGa                                                                         >  1:1486433/1‑101 (MQ=255)
                    gCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGAc                                                                        <  1:2196246/101‑1 (MQ=255)
                     cAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACa                                                                       <  2:2291642/101‑1 (MQ=255)
                         ccAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGAt                                                                   <  1:984232/101‑1 (MQ=255)
                         ccAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGAt                                                                   <  2:2691704/101‑1 (MQ=255)
                         ccAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGAt                                                                   <  2:289145/101‑1 (MQ=255)
                         ccAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGAt                                                                   <  1:3854063/101‑1 (MQ=255)
                           aaGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGTtct                                                                 >  1:4170440/1‑101 (MQ=255)
                           aaGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGAtct                                                                 <  1:2931583/101‑1 (MQ=255)
                           aaGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGAtct                                                                 >  1:224219/1‑101 (MQ=255)
                            aGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGAtctc                                                                <  1:1428074/101‑1 (MQ=255)
                            aGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGAtctc                                                                <  1:4091859/101‑1 (MQ=255)
                            aGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGAtctc                                                                <  1:1611451/101‑1 (MQ=255)
                             gCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCa                                                               >  1:261351/1‑101 (MQ=255)
                             gCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCa                                                               <  2:2792714/101‑1 (MQ=255)
                              cTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCat                                                              <  2:2644877/101‑1 (MQ=255)
                              cTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCat                                                              <  2:3807100/101‑1 (MQ=255)
                              cTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCat                                                              <  2:335186/101‑1 (MQ=255)
                                 gcgGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATAtt                                                           <  2:1682788/101‑1 (MQ=255)
                                  cgGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATAttt                                                          >  2:3498195/1‑101 (MQ=255)
                                   ggACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTg                                                         <  1:1862700/101‑1 (MQ=255)
                                   ggACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTg                                                         <  2:1486331/101‑1 (MQ=255)
                                    gACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGt                                                        >  1:3268341/1‑101 (MQ=255)
                                     aCCTGAGCCGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTa                                                       <  2:3409365/101‑1 (MQ=255)
                                      ccTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTaa                                                      <  2:101535/101‑1 (MQ=255)
                                       cTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTaaa                                                     <  1:7213/101‑1 (MQ=255)
                                        tGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAAc                                                    >  2:2521730/1‑101 (MQ=255)
                                          agaCGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACtt                                                  >  1:1248690/1‑101 (MQ=255)
                                          agaCGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCAACTTTCTAAACtt                                                  >  1:672684/1‑101 (MQ=255)
                                           gaCGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTc                                                 <  1:2446260/101‑1 (MQ=255)
                                            gcGCCGTCTGCGGCGGATGCGCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTcg                                                <  2:2199946/100‑1 (MQ=255)
                                            aCGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTcg                                                <  2:3846448/101‑1 (MQ=255)
                                             cGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCAGCCGACAGGATCTCATATTTGTAAACTTcgc                                               <  2:3879117/101‑1 (MQ=255)
                                                cGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTcgcccg                                            <  2:1843393/101‑1 (MQ=255)
                                                cGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTcgcccg                                            <  2:1103685/101‑1 (MQ=255)
                                                 gTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTcgcccgc                                           >  2:1724217/1‑101 (MQ=255)
                                                 gTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTcgcccgc                                           >  1:82666/1‑101 (MQ=255)
                                                 gTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTcgcccgc                                           >  1:2090037/1‑101 (MQ=255)
                                                   cTGCGGGGGTTGCTCGGCACCAAACCCCCCGGGATCGGCTTTGAGCGGCTGAACGACACGAACGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCa                                         <  2:673852/101‑1 (MQ=255)
                                                    tGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCAt                                        >  1:3477767/1‑101 (MQ=255)
                                                     gcggcgTATGCGCGGCCCCAAACCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCACCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATc                                       <  2:3630565/101‑1 (MQ=255)
                                                      cggcggATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCa                                      >  2:727744/1‑101 (MQ=255)
                                                       gggggATGCTCGGCGCCAAACCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGAAAACTTCGCCCGCCATCAg                                     <  2:4170178/98‑1 (MQ=255)
                                                           gATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGccc                                 >  1:2748750/1‑101 (MQ=255)
                                                           gATGCTCGGCGCCAAACCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGccc                                 <  1:316585/101‑1 (MQ=255)
                                                           gATCCTCGCCCCCAAACCCCCCCGGAGGGGCCTTGAGCGGCTGAACGACGCCAGCGGCCGACAGGATCTCATTTTTGAAAACTCCGCCCGCCATCAGGccc                                 <  2:1507677/101‑1 (MQ=255)
                                                            aTGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCg                                >  2:2244675/1‑101 (MQ=255)
                                                             tGCCCGGCCCCAAACCCCCCCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCgg                               <  1:2166707/101‑1 (MQ=255)
                                                              gCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGc                              >  2:2550516/1‑101 (MQ=255)
                                                              gCTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGc                              >  1:1039837/1‑101 (MQ=255)
                                                               cTCGGCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCa                             >  1:2795521/1‑101 (MQ=255)
                                                                 cGGCGCCAAACCCCCCCGGATCGGCCTTGAGCGGCTGAACGCCGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACg                           <  2:52494/101‑1 (MQ=255)
                                                                  ggCGCCAAATCCCACCGGATCTGCCTTGATCTGGTGAACAATGCCTTCGGCCGACAGGATCTCTTATTCTTAAACTTCGCCCGCTATCAGGGCAGGCAccg                          >  2:519769/1‑99 (MQ=255)
                                                                  ggCGCCAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGa                          >  1:2067690/1‑101 (MQ=255)
                                                                       cAAATCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGAtc                     >  2:813162/1‑101 (MQ=255)
                                                                         acccccACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTAAAACCTTCCCCCGCCATCAGGCCCGGCACGAAGAtctc                   <  1:1590872/98‑1 (MQ=255)
                                                                          accccccgggAGCGGCCTTGAGCGGCTGACCGACCCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTcc                  <  1:1814939/93‑1 (MQ=255)
                                                                          aTCCCACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTcc                  >  1:507172/1‑101 (MQ=255)
                                                                           ccccccgggATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTccc                 <  2:2277077/94‑1 (MQ=255)
                                                                            cccccgggATCGCCCTTGAGCGGCTGACCGACGCCAGCGGCCGACAGGATCTCATATTTGAAAACTTCCCCCGCCATCAGGCCCGGCACGAAGATCTCCCa                <  1:1575676/95‑1 (MQ=255)
                                                                             cccccGGATCGGCCTGGAGGGGCTGAACGACGCCATCGGCCGACAGGATTTAATTTTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCaa               <  1:359854/98‑1 (MQ=255)
                                                                             ccACCGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCaa               >  2:4194582/1‑101 (MQ=255)
                                                                               cccGGATCGGCCTTGAGCGGCTGAACGCCGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCCCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAAc             <  1:2521914/100‑1 (MQ=255)
                                                                               cccGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTAATATTTGTAAACTCCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAAc             <  1:3515522/100‑1 (MQ=255)
                                                                                 cGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAAccc           >  1:1856502/1‑101 (MQ=255)
                                                                                 cGGATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAAccc           >  2:2734711/1‑101 (MQ=255)
                                                                                   gtgcgtccTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGAAAACTCCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAAccccc         <  1:4186278/95‑1 (MQ=255)
                                                                                   gATCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAAccccc         >  2:4140073/1‑101 (MQ=255)
                                                                                   gATCGCCCTTGAGCGGCTGAACGACGCCAGCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAAccccc         <  1:1338668/101‑1 (MQ=255)
                                                                                    aTCGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACCAAGATCTCCCAAACCCCCg        >  2:2952079/1‑101 (MQ=255)
                                                                                     tCGGCCTTGAGCGGCTAAACGCCCCCACCGGCCGACAGGATCTCATTTTTGAAAACTTCCCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAACCCCCGt       <  1:2042487/101‑1 (MQ=255)
                                                                                     gcGGCCTTGAGGGGCTGAACGCGCCCAGGGGCCGACAGGATCTCATATTTGTAACCTCCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAACCCCCGt       <  2:2298128/100‑1 (MQ=255)
                                                                                      cGGCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGACCTCATATTTGTAAAATTCGCCCGCCATCAGGCCCGGCACGAAGATCACCCAAACCCCCGTc      >  1:1058034/1‑101 (MQ=255)
                                                                                       ggCCTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAACCCCCGTcg     >  1:2321674/1‑101 (MQ=255)
                                                                                         ccTTGAGCGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAACCCCCGTcgcg   >  1:3447913/1‑101 (MQ=255)
                                                                                               ||                                                                                              
GGCGCCGTCGCCCCACAGATGCAGTCCAAGCTCGCGGACCTGAGACGCCGTCTGCGGCGGATGCTCGGCGCCAAATCCCACCGGATCGGCCTTGAAAGGCTGAACGACGCCATCGGCCGACAGGATCTCATATTTGTAAACTTCGCCCGCCATCAGGCCCGGCACGAAGATCTCCCAAACCCCCGTCGCGC  >  NC_014034/1991948‑1992138

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG