Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,053,078 2 bp→GC 100% coding (4156‑4157/6198 nt) RCAP_rcc01900 ← hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,053,0780C→G100.0% 589.3 161R1386P (CGG→CCG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (85/76):  ref base (0/0):  total (85/76)
*NC_0140342,053,0790G→C100.0% 602.4 161R1386G (CGG→GGG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (85/76):  ref base (0/0):  total (85/76)

Only 100 of 174 total aligned reads displayed.

GGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACG  >  NC_014034/2052983‑2053173
                                                                                               ||                                                                                              
ggATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCg                                                                                            <  2:3320735/101‑1 (MQ=255)
 gATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCACCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGc                                                                                           <  1:918463/101‑1 (MQ=255)
  atttCGTTGCCCGCGCCGCCAAGGATCCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGcc                                                                                          <  2:4125279/98‑1 (MQ=38)
   tGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCg                                                                                         >  2:837461/1‑101 (MQ=255)
       gTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGa                                                                                     >  2:584794/1‑101 (MQ=255)
        ttccccCCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGAt                                                                                    <  1:2868034/98‑1 (MQ=38)
        ttGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGAt                                                                                    >  1:847468/1‑101 (MQ=255)
         tGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATg                                                                                   >  1:38176/1‑101 (MQ=255)
          gCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATgg                                                                                  <  1:3707003/101‑1 (MQ=255)
           cccGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgt                                                                                 <  1:4030909/101‑1 (MQ=255)
            ccGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgtg                                                                                <  2:2421435/101‑1 (MQ=255)
             cgcgCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgtgt                                                                               <  2:1342168/101‑1 (MQ=255)
             cgcgCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGATGTCATTGCCCGCGCCACCGTCCGGCGTGTCATCGCCCGCTTCGCCGAGGATGgtgt                                                                               >  1:3620537/1‑101 (MQ=38)
              gcgcCGCCAAGGATGCTTTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTc                                                                              <  2:1918858/101‑1 (MQ=255)
              gcgcCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATNgngtn                                                                              >  1:1163201/1‑100 (MQ=255)
              gcgcCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTc                                                                              <  1:1021576/101‑1 (MQ=255)
               cgccgcCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCg                                                                             >  1:1571994/1‑101 (MQ=255)
                 ccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGtt                                                                           <  1:2603215/101‑1 (MQ=255)
                  cgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTg                                                                          >  1:427848/1‑101 (MQ=255)
                    ccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGcc                                                                        <  2:2425789/101‑1 (MQ=255)
                     cAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGccc                                                                       >  1:779386/1‑101 (MQ=255)
                      aaGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCt                                                                      <  2:3293844/101‑1 (MQ=255)
                       aGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTc                                                                     >  1:1102695/1‑101 (MQ=255)
                         gATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTccc                                                                   >  1:1669975/1‑101 (MQ=255)
                         gATGCTGGCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTccc                                                                   <  2:947666/101‑1 (MQ=255)
                          aTGCTGTCGTCATCCAGCCCGCCGGTGATGCTGGCATTGCCCGCGCCACCGTCCACCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcccc                                                                  <  2:1659401/101‑1 (MQ=39)
                               gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                             >  1:3423444/1‑101 (MQ=255)
                               gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                             <  2:1989271/101‑1 (MQ=255)
                               gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                             >  1:970309/1‑101 (MQ=255)
                                tcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAg                                                            <  2:1037209/101‑1 (MQ=255)
                                  gtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGcc                                                          >  2:3369396/1‑100 (MQ=255)
                                  gtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCa                                                          >  2:3010046/1‑101 (MQ=255)
                                    cagccAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAcc                                                        <  2:4003530/98‑1 (MQ=255)
                                       ccAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAg                                                     <  1:1215370/101‑1 (MQ=255)
                                        ccgCCCGCCGGTTATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGt                                                    <  1:68797/99‑1 (MQ=255)
                                        ccgCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGt                                                    <  2:4212125/99‑1 (MQ=255)
                                        cAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGt                                                    <  1:350942/101‑1 (MQ=255)
                                         cgCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTc                                                   <  1:2892879/100‑1 (MQ=255)
                                         aGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAgcc                                                   >  1:3874669/1‑99 (MQ=255)
                                         aGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTc                                                   >  1:1527146/1‑101 (MQ=255)
                                         aGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTc                                                   <  1:1653379/101‑1 (MQ=255)
                                         aGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCATCCCCGCCCAGCACCGCGTc                                                   >  2:1269321/1‑101 (MQ=37)
                                          gCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCa                                                  >  2:3283969/1‑101 (MQ=255)
                                          gCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCa                                                  <  2:2353895/101‑1 (MQ=255)
                                             cgccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGTACCGAGTCATCg                                               <  1:2299367/101‑1 (MQ=255)
                                             cgccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCg                                               >  2:3026971/1‑101 (MQ=255)
                                                cgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGccc                                            >  2:317738/1‑101 (MQ=255)
                                                cgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGccc                                            <  1:1886499/101‑1 (MQ=255)
                                                cgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGccc                                            <  2:3620284/101‑1 (MQ=255)
                                                cgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGccc                                            <  1:1347227/101‑1 (MQ=255)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                           >  2:1842766/1‑101 (MQ=255)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                           >  1:3185835/1‑101 (MQ=255)
                                                  gTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGa                                          <  2:58972/101‑1 (MQ=255)
                                                  gTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGa                                          <  2:851340/101‑1 (MQ=255)
                                                   tgaaggcgccATTGCCCGCGCCACCGTCCAGCGTGACATCGCCCGCTTCGCCGAGCATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAg                                         <  2:500168/92‑1 (MQ=255)
                                                   tGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAg                                         >  1:2997407/1‑101 (MQ=255)
                                                    gATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGc                                        >  1:990971/1‑101 (MQ=255)
                                                       gCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCg                                     <  2:2547281/101‑1 (MQ=255)
                                                        cTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGc                                    >  1:1479931/1‑101 (MQ=255)
                                                          gTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCt                                  >  2:3180686/1‑101 (MQ=255)
                                                          gTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCt                                  <  2:1182037/101‑1 (MQ=255)
                                                           tCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtc                                 <  1:33813/101‑1 (MQ=255)
                                                           tCATTGCCCGCGCCACCGCCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtc                                 <  2:3802963/101‑1 (MQ=255)
                                                            cATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcg                                <  2:3015204/101‑1 (MQ=255)
                                                            cATTGCCCGCACCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcg                                >  1:3658227/1‑101 (MQ=255)
                                                              ttGCCCGCGCCACCGTCCAGCGTGCCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgat                              <  2:3874410/101‑1 (MQ=255)
                                                               tGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatc                             >  2:3337830/1‑101 (MQ=255)
                                                                gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                            >  2:3633242/1‑101 (MQ=255)
                                                                gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                            >  2:2926063/1‑101 (MQ=255)
                                                                 cccGCGCCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcga                           <  1:918038/101‑1 (MQ=255)
                                                                   cgcgCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAt                         <  2:1769516/101‑1 (MQ=255)
                                                                   cgccCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAt                         <  2:2292957/101‑1 (MQ=255)
                                                                    gcgcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtc                        <  1:3141539/101‑1 (MQ=255)
                                                                      gcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgt                      >  1:360250/1‑101 (MQ=255)
                                                                        ccccGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtcg                    <  1:4107594/99‑1 (MQ=255)
                                                                         aCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGGGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGc                   >  1:813979/1‑101 (MQ=255)
                                                                          ccGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGcc                  <  1:2020776/101‑1 (MQ=255)
                                                                          ccGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGcc                  >  1:2439102/1‑101 (MQ=255)
                                                                            gTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCgg                >  1:3199051/1‑101 (MQ=255)
                                                                            gTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCgg                <  2:3107037/101‑1 (MQ=255)
                                                                             tCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCCCCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGt               <  2:779308/101‑1 (MQ=255)
                                                                             tCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGt               >  2:887785/1‑101 (MQ=255)
                                                                             tCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGCCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGt               >  1:785368/1‑101 (MQ=255)
                                                                              ccAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTg              >  1:1603322/1‑101 (MQ=255)
                                                                              ccAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTg              >  1:3464806/1‑101 (MQ=255)
                                                                              ccAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCCCCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTg              <  1:3085077/101‑1 (MQ=255)
                                                                               cAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAACGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGc             >  1:652416/1‑101 (MQ=255)
                                                                               cAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTATCGATGCCCTCCCCGCCCAGAACCGAGGCATCGCCCGAGCCCGCATCGATCGAATAGTCGCCGGTGc             >  2:1079388/1‑101 (MQ=255)
                                                                                aGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGcc            >  1:3775768/1‑101 (MQ=255)
                                                                                aGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGcc            <  1:2646100/101‑1 (MQ=255)
                                                                                 gCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGccc           <  2:3423199/101‑1 (MQ=255)
                                                                                 gCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGccc           <  2:661731/101‑1 (MQ=255)
                                                                                   ggtgccacGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgc         <  2:3230518/94‑1 (MQ=255)
                                                                                    tgtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgcg        >  2:3471924/1‑101 (MQ=255)
                                                                                    tgtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgcg        >  1:2624146/1‑101 (MQ=255)
                                                                                     gtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGt       >  2:163745/1‑101 (MQ=255)
                                                                                       caccGCCCGCTTCGCCGAGGATGTTGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCCCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTc     <  2:1425577/98‑1 (MQ=255)
                                                                                       cATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCTCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTc     >  1:3227887/1‑101 (MQ=255)
                                                                                        tccGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCa    <  2:2177525/99‑1 (MQ=255)
                                                                                          cGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACg  >  2:321361/1‑101 (MQ=255)
                                                                                               ||                                                                                              
GGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACG  >  NC_014034/2052983‑2053173

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG