Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,157,926 2 bp→AC 100% intergenic (‑298/‑483) RCAP_rcc02004 ← / → RCAP_rcc02005 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,157,9260C→A100.0% 47.9 215intergenic (‑298/‑484)RCAP_rcc02004/RCAP_rcc02005hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (96/100):  ref base (17/2):  total (113/102)
Fisher's exact test strand distribution p-value = 5.70e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.09e-01
*NC_0140342,157,9270G→C100.0% 40.6 215intergenic (‑299/‑483)RCAP_rcc02004/RCAP_rcc02005hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (96/101):  ref base (17/1):  total (113/102)
Fisher's exact test strand distribution p-value = 1.09e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGT  >  NC_014034/2157834‑2158020
                                                                                             ||                                                                                             
ctcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCg                                                                                        >  2:846365/1‑101 (MQ=255)
  cccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGt                                                                                      <  1:1075054/101‑1 (MQ=255)
   ccAGCATACCAAACCGCTGGGGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGtt                                                                                     <  1:216773/101‑1 (MQ=38)
   ccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGtt                                                                                     >  1:3992653/1‑101 (MQ=255)
   ccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGtt                                                                                     >  1:446953/1‑101 (MQ=255)
   ccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGtt                                                                                     <  1:1957231/101‑1 (MQ=255)
   ccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGtt                                                                                     >  1:1814093/1‑101 (MQ=255)
    cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                    <  2:3295490/101‑1 (MQ=255)
    cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                    <  2:1427318/101‑1 (MQ=255)
    cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                    <  2:3884770/101‑1 (MQ=255)
     aGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCa                                                                                   <  1:38304/101‑1 (MQ=255)
       cATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGt                                                                                 >  2:1531272/1‑101 (MQ=255)
       cATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGt                                                                                 >  2:3772423/1‑101 (MQ=255)
        aTACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTc                                                                                >  1:1215344/1‑101 (MQ=255)
         tACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTcc                                                                               >  2:4103810/1‑101 (MQ=255)
            cAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTccgcc                                                                            <  1:559462/101‑1 (MQ=255)
              aaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgt                                                                          <  1:489251/101‑1 (MQ=255)
                 cGCTGGAGACGATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtcgt                                                                       <  2:1973519/101‑1 (MQ=37)
                  gCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCgtcgtc                                                                      >  2:220147/1‑101 (MQ=255)
                   cTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCt                                                                     >  2:3217842/1‑101 (MQ=255)
                    tGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCtt                                                                    >  2:1148907/1‑101 (MQ=255)
                    tGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCtt                                                                    <  1:882185/101‑1 (MQ=255)
                    tGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCtt                                                                    >  1:3170736/1‑101 (MQ=255)
                          ccATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGt                                                              <  1:2331436/101‑1 (MQ=255)
                           cATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTg                                                             <  2:3028383/101‑1 (MQ=255)
                            aTCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGa                                                            >  2:2414805/1‑101 (MQ=255)
                            aTCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGCGa                                                            >  1:2269114/1‑101 (MQ=38)
                              cAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACg                                                          >  1:3144648/1‑101 (MQ=255)
                               aGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGt                                                         <  1:3226964/101‑1 (MQ=255)
                                tcttCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTg                                                        <  2:1182689/99‑1 (MQ=255)
                                ggTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTg                                                        >  1:472209/1‑101 (MQ=255)
                                 gTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGa                                                       <  1:81280/101‑1 (MQ=255)
                                  ttCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAg                                                      <  1:2893931/101‑1 (MQ=255)
                                  ttCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAg                                                      >  1:1516017/1‑101 (MQ=255)
                                  ttCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAg                                                      >  2:1808969/1‑101 (MQ=255)
                                    cAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGc                                                    >  2:1242950/1‑101 (MQ=255)
                                     aGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAggga                                                   >  1:2476744/1‑99 (MQ=255)
                                     aGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGcc                                                   >  1:2497817/1‑100 (MQ=255)
                                     aGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCa                                                   <  1:2064800/101‑1 (MQ=255)
                                      gTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCAc                                                  <  2:2118010/101‑1 (MQ=255)
                                      gTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCAc                                                  <  1:1634706/101‑1 (MQ=255)
                                       tCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCAcc                                                 >  2:2914643/1‑101 (MQ=255)
                                       tCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCAcc                                                 >  1:1883977/1‑101 (MQ=255)
                                        ccgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCt                                                <  1:984153/101‑1 (MQ=255)
                                        ccgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCt                                                >  1:1601703/1‑101 (MQ=255)
                                        ccgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCt                                                >  1:3670571/1‑101 (MQ=255)
                                        ccgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCt                                                >  2:372992/1‑101 (MQ=255)
                                         cgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTa                                               <  1:3513791/101‑1 (MQ=255)
                                         cgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTa                                               <  2:1122965/101‑1 (MQ=255)
                                            cgtcgtCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAAc                                            >  2:1523428/1‑101 (MQ=255)
                                             gtcgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACa                                           <  2:1714143/101‑1 (MQ=255)
                                             gtcgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACa                                           <  2:2497633/101‑1 (MQ=255)
                                              tcgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACaa                                          <  2:2595442/101‑1 (MQ=255)
                                              tcgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACaa                                          <  2:3535737/101‑1 (MQ=255)
                                               cgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAag                                         <  1:1000332/101‑1 (MQ=255)
                                                ttcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAaga                                        >  2:443772/2‑101 (MQ=38)
                                                gtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAaga                                        <  2:2702586/101‑1 (MQ=255)
                                                  cTTGGCGGGGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                      >  1:910118/1‑101 (MQ=38)
                                                   ttGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGGACCTAAACAAGAGGt                                     >  2:2542882/1‑101 (MQ=38)
                                                   ttGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTCAACAAGAGGt                                     >  2:705407/1‑101 (MQ=38)
                                                   ttGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACACGAGGt                                     <  2:1872134/101‑1 (MQ=38)
                                                   ttGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGt                                     >  1:353302/1‑101 (MQ=255)
                                                    tGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGtt                                    <  2:3485636/101‑1 (MQ=255)
                                                      gCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCt                                  >  1:3985939/1‑101 (MQ=255)
                                                        ggggatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTAc                                <  2:2047329/98‑1 (MQ=38)
                                                         gtgatgtgatGCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACg                               >  2:49852/1‑101 (MQ=255)
                                                         gtgatgtgatGCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACg                               >  2:3075518/1‑101 (MQ=255)
                                                          tgatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGNCGTNAGGCACCTAAACAAGAGGTTCTACGa                              >  1:2864952/1‑101 (MQ=38)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                             <  1:1155324/101‑1 (MQ=255)
                                                            atgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                            >  2:1909620/1‑101 (MQ=255)
                                                            atgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                            >  1:2599540/1‑101 (MQ=255)
                                                            atgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                            <  1:1523532/101‑1 (MQ=255)
                                                             tgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                           >  1:2691468/1‑101 (MQ=255)
                                                             tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                           <  1:4181823/101‑1 (MQ=255)
                                                             tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                           <  1:846526/101‑1 (MQ=255)
                                                              gtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGcc                          <  1:3434318/101‑1 (MQ=255)
                                                              gtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGcc                          <  1:3234779/101‑1 (MQ=255)
                                                              gtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGcc                          <  1:4104071/101‑1 (MQ=255)
                                                               tgaggCACAGGAGAGACCGATGAAAATC.AACGTCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                         <  2:3873829/101‑1 (MQ=38)
                                                                 atgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGaa                       >  2:2169730/1‑101 (MQ=255)
                                                                  tgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGaaa                      <  1:2384134/101‑1 (MQ=255)
                                                                  tgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGaaa                      <  2:766899/101‑1 (MQ=255)
                                                                     acaGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAggg                   >  1:2535672/1‑101 (MQ=255)
                                                                     acaGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAggg                   >  2:4171752/1‑101 (MQ=255)
                                                                      caGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAgggg                  <  2:3256187/101‑1 (MQ=255)
                                                                       aGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGt                 >  2:1301262/1‑101 (MQ=255)
                                                                       aGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGGCGTCTTGGCGGTGACGTGTGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGt                 >  1:1761668/1‑101 (MQ=255)
                                                                        ggAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGtt                <  1:49867/101‑1 (MQ=255)
                                                                         gagagaCCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTc               >  1:2119517/1‑101 (MQ=255)
                                                                          agagaCCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCa              >  2:2244920/1‑101 (MQ=255)
                                                                           gagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAg             >  1:1472464/1‑101 (MQ=255)
                                                                            agaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGt            >  2:3155528/1‑101 (MQ=255)
                                                                              aCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTcc          <  1:2766786/101‑1 (MQ=255)
                                                                                  aTGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgccg      <  1:1980669/101‑1 (MQ=255)
                                                                                   tGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgt     >  2:731432/1‑101 (MQ=255)
                                                                                     aaaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcg   >  1:1137291/1‑101 (MQ=255)
                                                                                     aaaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGGGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGGTCAGTCCGCcgtcg   >  2:2475329/1‑101 (MQ=38)
                                                                                      aaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGTGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcgt  <  1:2946436/101‑1 (MQ=38)
                                                                                      aaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcgt  >  2:453202/1‑101 (MQ=255)
                                                                                      aaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACATAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcgt  >  2:2269299/1‑101 (MQ=38)
                                                                                             ||                                                                                             
CTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGT  >  NC_014034/2157834‑2158020

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG