Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,185 2 bp→GC 100% coding (874‑875/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1850C→G100.0% 630.2 168R292G (CGC→GGC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (77/91):  ref base (0/0):  total (77/91)
*NC_0140342,180,1860G→C100.0% 639.4 169R292P (CGC→CCC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (77/92):  ref base (0/0):  total (77/92)

Only 100 of 170 total aligned reads displayed.

ATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.C.GGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACC  >  NC_014034/2180093‑2180283
                                                                                                ||                                                                                                 
aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAg                                                                                               >  2:3597531/1‑101 (MQ=255)
aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAg                                                                                               >  2:741568/1‑101 (MQ=255)
aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAg                                                                                               <  2:1546986/101‑1 (MQ=255)
 tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGc                                                                                              <  1:503107/101‑1 (MQ=255)
 tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGc                                                                                              <  1:58369/101‑1 (MQ=255)
    gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATg                                                                                           >  1:793221/1‑101 (MQ=255)
     aCCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATgg                                                                                          >  2:3683523/1‑101 (MQ=255)
      ccGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGc                                                                                         >  2:2978626/1‑101 (MQ=255)
       cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCa                                                                                        <  2:2280521/101‑1 (MQ=255)
        gTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCaa                                                                                       >  2:450395/1‑101 (MQ=255)
        gTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCaa                                                                                       <  1:3994962/101‑1 (MQ=255)
         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAAc                                                                                      <  2:2424325/101‑1 (MQ=255)
         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAAc                                                                                      >  2:3534658/1‑101 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACt                                                                                     <  2:3332053/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACt                                                                                     <  1:3344606/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACt                                                                                     <  1:1654648/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACt                                                                                     <  1:1926524/101‑1 (MQ=255)
           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTg                                                                                    >  2:124868/1‑101 (MQ=255)
           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTg                                                                                    <  2:836530/101‑1 (MQ=255)
           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTg                                                                                    <  2:2545978/101‑1 (MQ=255)
           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTg                                                                                    <  1:1658396/101‑1 (MQ=255)
           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTg                                                                                    >  1:3821248/1‑101 (MQ=255)
             cgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGc                                                                                  >  2:3718368/1‑101 (MQ=255)
             cgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGc                                                                                  <  1:3683777/101‑1 (MQ=255)
             cgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGc                                                                                  <  1:2055146/101‑1 (MQ=255)
             cgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGc                                                                                  <  2:3015938/101‑1 (MQ=255)
               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGctt                                                                                >  2:2497296/1‑100 (MQ=255)
               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGctc                                                                                >  1:931493/1‑101 (MQ=255)
               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGctc                                                                                <  2:1780279/101‑1 (MQ=255)
               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGctc                                                                                <  2:724858/101‑1 (MQ=255)
                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                                             >  2:242685/1‑101 (MQ=255)
                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                                             >  1:3068207/1‑101 (MQ=255)
                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                                             <  1:4160584/101‑1 (MQ=255)
                    ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGc                                                                           <  2:1033949/101‑1 (MQ=255)
                    ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGc                                                                           <  1:1277207/101‑1 (MQ=255)
                    ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGc                                                                           >  1:1151799/1‑101 (MQ=255)
                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                         <  2:1867090/101‑1 (MQ=255)
                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                         <  2:17366/101‑1 (MQ=255)
                       gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                        >  2:2481278/1‑101 (MQ=255)
                           ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                                                    <  2:1567036/101‑1 (MQ=255)
                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                                   <  2:906391/101‑1 (MQ=255)
                             aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                                  <  2:3067982/101‑2 (MQ=255)
                              aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                 <  2:634000/101‑3 (MQ=255)
                               ccccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcg                                                                >  2:3715006/1‑98 (MQ=255)
                                 ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                                             >  1:1033406/1‑101 (MQ=255)
                                 ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                                             <  2:3147467/101‑1 (MQ=255)
                                  cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                            >  1:2666745/1‑101 (MQ=255)
                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                           >  1:663193/1‑100 (MQ=255)
                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                           >  2:1198791/1‑100 (MQ=255)
                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                          <  1:563187/101‑3 (MQ=255)
                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                         <  2:3370631/101‑4 (MQ=255)
                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                         <  2:873864/101‑4 (MQ=255)
                                      gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                        >  2:2730006/1‑97 (MQ=255)
                                         tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                                    >  1:2413043/1‑101 (MQ=255)
                                         tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                                    >  2:1687423/1‑101 (MQ=255)
                                          cGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                                                   <  1:2632187/101‑1 (MQ=255)
                                             ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                <  1:3435983/101‑1 (MQ=255)
                                             ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                >  2:3991602/1‑101 (MQ=255)
                                              cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                                               <  1:1826822/101‑1 (MQ=255)
                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGGACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                              <  1:3606602/101‑1 (MQ=255)
                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                              >  1:1018891/1‑101 (MQ=255)
                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                              >  1:702758/1‑101 (MQ=255)
                                                 ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                            >  1:141214/1‑101 (MQ=255)
                                                 ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                            <  1:191953/101‑1 (MQ=255)
                                                 ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                            >  1:2008488/1‑101 (MQ=255)
                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                           <  1:2798514/101‑1 (MQ=255)
                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                           <  1:2794904/101‑1 (MQ=255)
                                                   ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                          <  2:935902/101‑1 (MQ=255)
                                                   ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                          <  2:4228651/101‑1 (MQ=255)
                                                   ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.CTGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                          <  1:3571147/101‑1 (MQ=255)
                                                    cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                         >  1:3091030/1‑101 (MQ=255)
                                                    cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                         <  1:2202661/101‑1 (MQ=255)
                                                       tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                                      >  1:2274318/1‑99 (MQ=255)
                                                        cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                     <  2:1263129/101‑4 (MQ=255)
                                                          tGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                  >  1:2937644/1‑101 (MQ=255)
                                                          tGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                  <  2:3820991/101‑1 (MQ=255)
                                                           gTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                 >  1:435007/1‑101 (MQ=255)
                                                            tcggcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                                <  2:41204/101‑1 (MQ=255)
                                                             cggcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAgg                               >  2:2023038/1‑101 (MQ=255)
                                                               gcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                             <  1:1690184/101‑1 (MQ=255)
                                                               gcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggag                             <  1:2978848/101‑3 (MQ=255)
                                                                cttcgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcgg                            >  2:323533/7‑101 (MQ=255)
                                                                   cgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGgcgc                         >  2:164132/4‑101 (MQ=255)
                                                                    gcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                        <  2:931408/99‑1 (MQ=255)
                                                                    gcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                        <  1:877928/99‑1 (MQ=255)
                                                                     cgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                       >  1:706750/2‑101 (MQ=255)
                                                                     cgcgggcgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                       <  1:3798636/93‑1 (MQ=255)
                                                                      gctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                      <  1:1970812/101‑1 (MQ=255)
                                                                          gcgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                  <  2:2666539/98‑1 (MQ=255)
                                                                              cGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGAAATtcgtc              >  2:64083/1‑101 (MQ=255)
                                                                               gAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg             >  1:3203728/1‑101 (MQ=255)
                                                                                aaCAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa            >  1:3005294/1‑101 (MQ=255)
                                                                                 aCAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGaa           >  2:1927128/1‑101 (MQ=255)
                                                                                  cAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt          <  1:4014685/101‑1 (MQ=255)
                                                                                  cAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTAGAA.CCTCGCC.ATTCCGACGGGCACGC.AAAAGGTCGAGGCGACGCTGAAATTCGTCGAAt          >  2:1376438/1‑101 (MQ=255)
                                                                                    aggcc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg        <  2:1064376/97‑1 (MQ=255)
                                                                                    aggcc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg        <  2:793143/97‑1 (MQ=255)
                                                                                       cc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg     <  1:813754/100‑1 (MQ=255)
                                                                                          ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc   >  1:1281733/1‑101 (MQ=255)
                                                                                           gtgtCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAcc  <  1:39221/101‑1 (MQ=255)
                                                                                                ||                                                                                                 
ATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.C.GGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACC  >  NC_014034/2180093‑2180283

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG