Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,620,690 2 bp→CT 100% coding (1050‑1051/1188 nt) livJ → branched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,620,6900T→C100.0% 782.0 203P350P (CCT→CCC) livJbranched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand):  new base (112/91):  ref base (0/0):  total (112/91)
*NC_0140342,620,6910A→T100.0% 799.5 203N351Y (AAC→TAC) livJbranched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand):  new base (112/91):  ref base (0/0):  total (112/91)

Only 100 of 203 total aligned reads displayed.

GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785
                                                                                               ||                                                                                              
gAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACAt                                                                                            >  1:2070566/1‑101 (MQ=255)
 aaTATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATg                                                                                           >  1:3898172/1‑101 (MQ=255)
  atatatCCTGCAGACGCCGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGa                                                                                          >  1:62958/1‑101 (MQ=255)
    atatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAg                                                                                        >  2:3679229/1‑101 (MQ=255)
     tatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAgg                                                                                       >  2:194133/1‑101 (MQ=255)
     tatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAgg                                                                                       >  2:1734009/1‑101 (MQ=255)
      atCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAggg                                                                                      >  2:386759/1‑101 (MQ=255)
      atCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAggg                                                                                      <  1:2136549/101‑1 (MQ=255)
       tCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGc                                                                                     >  1:1328251/1‑101 (MQ=255)
       tCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGc                                                                                     >  1:282097/1‑101 (MQ=255)
        ccTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCg                                                                                    <  1:1269459/101‑1 (MQ=255)
         cTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGa                                                                                   >  2:3747818/1‑101 (MQ=255)
         cTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGa                                                                                   >  2:1297992/1‑101 (MQ=255)
          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  >  2:821939/1‑101 (MQ=255)
          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  <  2:1601244/101‑1 (MQ=255)
          cggaGACGCCGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  <  2:3452927/98‑1 (MQ=255)
            caaaCGCTGAAGGCCGACCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGGCCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGc                                                                                <  2:520279/98‑1 (MQ=255)
            cAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGc                                                                                >  1:1955746/1‑101 (MQ=255)
            cAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGc                                                                                <  1:3926866/101‑1 (MQ=255)
            cAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGc                                                                                <  2:1896097/101‑1 (MQ=255)
             aGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCg                                                                               >  1:472603/1‑101 (MQ=255)
             aGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCg                                                                               >  2:3338796/1‑101 (MQ=255)
             aGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCg                                                                               >  1:3713448/1‑101 (MQ=255)
              gACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGa                                                                              <  1:4209932/101‑1 (MQ=255)
              gACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGa                                                                              <  2:2001961/101‑1 (MQ=255)
                 gCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGc                                                                           >  2:2341317/1‑101 (MQ=255)
                 gCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGc                                                                           >  2:3977614/1‑101 (MQ=255)
                   tGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         <  1:257049/101‑1 (MQ=255)
                   tGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         >  2:674241/1‑101 (MQ=255)
                       aGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCt                                                                     >  2:2938540/1‑101 (MQ=255)
                       aGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCt                                                                     <  2:3192329/101‑1 (MQ=255)
                        gCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTa                                                                    >  1:688504/1‑101 (MQ=255)
                         ccGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTAt                                                                   >  2:1904601/1‑101 (MQ=255)
                           gATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 <  2:1502346/101‑1 (MQ=255)
                           gATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 >  1:412243/1‑101 (MQ=255)
                           gATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 <  1:859642/101‑1 (MQ=255)
                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                <  1:1378452/101‑1 (MQ=255)
                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                <  2:355551/101‑1 (MQ=255)
                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                >  2:3209132/1‑101 (MQ=255)
                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                <  1:2280522/101‑1 (MQ=255)
                             tCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCg                                                               <  1:4032593/101‑1 (MQ=255)
                              ccGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCgg                                                              <  1:757548/101‑1 (MQ=255)
                               cGAAGGGCCTGACTGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGc                                                             <  1:3640356/101‑1 (MQ=255)
                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            <  2:3047017/101‑1 (MQ=255)
                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            >  2:3905871/1‑101 (MQ=255)
                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            >  1:2367881/1‑101 (MQ=255)
                                   gggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcacca                                                         >  2:2474782/1‑101 (MQ=255)
                                    ggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcaccac                                                        >  2:2986991/1‑101 (MQ=255)
                                     gCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCaccacc                                                       <  1:1486718/101‑1 (MQ=255)
                                        tGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCg                                                    >  1:1050236/1‑101 (MQ=255)
                                            cGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCg                                                <  1:2938762/101‑1 (MQ=255)
                                                     cgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGc                                       <  2:2745061/101‑1 (MQ=255)
                                                     cgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGc                                       <  1:1922354/101‑1 (MQ=255)
                                                     cgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGc                                       >  1:2596978/1‑101 (MQ=255)
                                                        cTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGc                                    <  2:414435/101‑1 (MQ=255)
                                                         ttCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCa                                   >  2:2892112/1‑101 (MQ=255)
                                                         ttCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCa                                   <  1:2899963/101‑1 (MQ=255)
                                                         ttCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCa                                   <  2:617586/101‑1 (MQ=255)
                                                          tCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAg                                  >  1:1991991/1‑101 (MQ=255)
                                                          tCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAg                                  <  1:279718/101‑1 (MQ=255)
                                                           cAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGc                                 <  2:1470948/101‑1 (MQ=255)
                                                            aaGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCt                                >  2:135445/1‑101 (MQ=255)
                                                            aaGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCt                                >  2:3286446/1‑101 (MQ=255)
                                                             aGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTg                               >  1:2679231/1‑101 (MQ=255)
                                                              gACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGt                              >  1:1176443/1‑101 (MQ=255)
                                                                ccGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCg                            >  2:3850977/1‑101 (MQ=255)
                                                                ccGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCg                            >  2:1575066/1‑101 (MQ=255)
                                                                ccGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCg                            >  1:3954751/1‑101 (MQ=255)
                                                                 cgcgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCgg                           >  2:100781/1‑101 (MQ=255)
                                                                  gcgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGt                          <  2:1955620/101‑1 (MQ=255)
                                                                   cgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTg                         <  1:1673964/101‑1 (MQ=255)
                                                                    gATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGc                        >  2:3638519/1‑101 (MQ=255)
                                                                    gATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGc                        >  2:1706747/1‑101 (MQ=255)
                                                                     aTCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGcc                       >  2:4011208/1‑101 (MQ=255)
                                                                        gACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCt                    <  2:1748261/101‑1 (MQ=255)
                                                                        gACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCt                    <  2:1599139/101‑1 (MQ=255)
                                                                           aGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgt                 >  2:3473049/1‑101 (MQ=255)
                                                                           aGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGGGCCggccgt                 >  1:4133007/1‑96 (MQ=255)
                                                                            gTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtg                <  1:3328419/101‑1 (MQ=255)
                                                                            gTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtg                <  2:2813436/101‑1 (MQ=255)
                                                                            gTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCCCgtg                >  2:587629/1‑101 (MQ=255)
                                                                            gTTTCCCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCCCCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtg                <  1:1558878/101‑1 (MQ=255)
                                                                             tttCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtgg               >  1:2550075/1‑101 (MQ=255)
                                                                               tCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtggtg             <  2:1126148/101‑1 (MQ=255)
                                                                               tCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtggtg             <  1:1896420/101‑1 (MQ=255)
                                                                                cgccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGa            >  2:2221787/1‑101 (MQ=255)
                                                                                cgccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGa            <  1:1140046/101‑1 (MQ=255)
                                                                                cgccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGa            <  1:2618338/101‑1 (MQ=255)
                                                                                 gccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGaa           <  1:1207112/101‑1 (MQ=255)
                                                                                 gccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGaa           <  2:208664/101‑1 (MQ=255)
                                                                                  ccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAAc          <  2:370783/101‑1 (MQ=255)
                                                                                  ccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAAc          >  2:2385823/1‑101 (MQ=255)
                                                                                  ccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAAc          <  1:2451863/101‑1 (MQ=255)
                                                                                      gccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAAt      >  1:1775169/1‑101 (MQ=255)
                                                                                        cgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATAc    >  2:2642996/1‑101 (MQ=255)
                                                                                        cgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATAc    <  2:2820524/101‑1 (MQ=255)
                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   >  2:1203473/1‑101 (MQ=255)
                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   >  1:3086889/1‑101 (MQ=255)
                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   <  1:3721963/101‑1 (MQ=255)
                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACaa  >  1:3836257/1‑101 (MQ=255)
                                                                                               ||                                                                                              
GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG