Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,747,876 2 bp→GC 100% intergenic (‑208/+84) RCAP_rcc02553 ← / ← RCAP_rcc02554 hypothetical protein/NmrA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,747,8760C→G100.0% 57.1 22intergenic (‑208/+85)RCAP_rcc02553/RCAP_rcc02554hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (21/1):  ref base (0/0):  total (21/1)
*NC_0140342,747,8770T→C100.0% 64.3 24intergenic (‑209/+84)RCAP_rcc02553/RCAP_rcc02554hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (23/1):  ref base (0/0):  total (23/1)

CGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAGC  >  NC_014034/2747804‑2747970
                                                                           ||                                                                                             
cgccaggcccGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGCCCGG.GCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTc                                                                      <  2:3769969/93‑1 (MQ=17)
                     caccacAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCtgtg                                                 >  2:3225047/1‑101 (MQ=14)
                     caccacAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCtgtg                                                 >  2:2589593/1‑101 (MQ=14)
                                     gcccggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg                                 >  1:2680314/1‑101 (MQ=18)
                                       ccggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt                               >  1:991505/1‑101 (MQ=18)
                                        cggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                              >  1:2270128/1‑101 (MQ=18)
                                         ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                             >  2:177914/1‑101 (MQ=18)
                                         ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                             >  1:349115/1‑101 (MQ=18)
                                              cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt                         >  1:81200/3‑101 (MQ=21)
                                                 cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGACCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGACTTGGc                      >  2:2129027/1‑101 (MQ=21)
                                                  gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc                     >  2:2885984/4‑101 (MQ=21)
                                                   ggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCa                    >  1:3278926/3‑101 (MQ=21)
                                                     cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAg                  >  1:3681922/1‑101 (MQ=21)
                                                     cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAg                  >  1:3489626/1‑101 (MQ=21)
                                                      gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg                 >  2:2985774/1‑101 (MQ=21)
                                                      gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg                 >  1:855434/1‑101 (MQ=21)
                                                      gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg                 >  1:286273/1‑101 (MQ=21)
                                                          aGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAg             >  1:728934/1‑101 (MQ=21)
                                                          aGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAg             >  1:3818703/1‑101 (MQ=21)
                                                             gcccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTc          >  1:4010350/1‑101 (MQ=21)
                                                             gcccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTc          >  1:1967331/1‑101 (MQ=21)
                                                               ccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCAt        >  1:1851148/1‑101 (MQ=21)
                                                                   cgggccgggCGCTGCCCGCGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCa    >  2:2613538/3‑101 (MQ=255)
                                                                     ggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAgc  >  1:461955/1‑101 (MQ=255)
                                                                           ||                                                                                             
CGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAGC  >  NC_014034/2747804‑2747970

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG