STRAIN: SE8R2

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breseq  version 0.22  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSE8R2_1_sequence.txt4,236,725427,909,225100.0%101.0 bases101 bases95.6%
errorsSE8R2_2_sequence.txt4,236,463427,882,763100.0%101.0 bases101 bases94.7%
total8,473,188855,791,988100.0%101.0 bases101 bases95.2%

Reference Sequence Information

seq idlengthfit meanfit dispersiondescription
coveragedistributionNC_0140343,738,958213.23.0Rhodobacter capsulatus SB 1003 chromosome, complete genome.
coveragedistributionNC_014035132,962142.01.9Rhodobacter capsulatus SB 1003 plasmid pRCB133, complete sequence.
total3,871,920

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

Junction Evidence

reference sequencepr(no read start)
NC_0140340.39563
NC_0140350.52953

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Polymorphism E-value cutoff2
Polymorphism frequency cutoffOFF
Polymorphism minimum coverage each strandOFF
Polymorphism homopolymer length cutoffOFF
Polymorphism bias cutoff0
Polymorphism predict indel polymorphismsOFF

Execution Times

stepstartendelapsed
Read and reference sequence file input10:35:58 20 Mar 201310:39:18 20 Mar 20133 minutes 20 seconds
Read alignment to reference genome10:39:19 20 Mar 201311:17:26 20 Mar 201338 minutes 7 seconds
Preprocessing alignments for candidate junction identification11:17:26 20 Mar 201311:24:28 20 Mar 20137 minutes 2 seconds
Preliminary analysis of coverage distribution11:24:29 20 Mar 201311:33:49 20 Mar 20139 minutes 20 seconds
Identifying junction candidates11:33:49 20 Mar 201311:33:58 20 Mar 20139 seconds
Re-alignment to junction candidates11:33:58 20 Mar 201311:46:27 20 Mar 201312 minutes 29 seconds
Resolving alignments with junction candidates11:46:27 20 Mar 201311:57:24 20 Mar 201310 minutes 57 seconds
Creating BAM files11:57:24 20 Mar 201312:05:53 20 Mar 20138 minutes 29 seconds
Tabulating error counts12:05:53 20 Mar 201312:08:55 20 Mar 20133 minutes 2 seconds
Re-calibrating base error rates12:08:55 20 Mar 201312:08:59 20 Mar 20134 seconds
Examining read alignment evidence12:08:59 20 Mar 201312:41:13 20 Mar 201332 minutes 14 seconds
Polymorphism statistics12:41:13 20 Mar 201312:41:24 20 Mar 201311 seconds
Output12:41:24 20 Mar 201312:58:18 20 Mar 201316 minutes 54 seconds
Total 2 hours 22 minutes 18 seconds