BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,208,437	Δ1 bp	2.5%	coding (867/948 nt)	pfkB ←	6‑phosphofructokinase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,208,437	0	C→.	2.5%	4.3 0.0 ‑0.6	163	coding (867/948 nt)	pfkB	6‑phosphofructokinase
Reads supporting (aligned to +/- strand): new base (2/2): ref base (95/64): total (97/66)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.45e-01

Only 100 of 225 total aligned reads displayed.


TCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCG  >  NC_014034/1208341‑1208536


tCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccg                                                                                                 >  2:2262413/1‑101 (MQ=255)


  cccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgcc                                                                                               <  1:3819470/101‑1 (MQ=255)


   ccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAAGCCGGGGGTGGGTGTCATCAccgccaccg                                                                                              >  1:2374189/1‑101 (MQ=255)


      gTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccg                                                                                           <  1:4397815/101‑1 (MQ=255)


      gTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccg                                                                                           >  1:106341/1‑101 (MQ=255)


       tttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgc                                                                                          >  2:1932605/1‑101 (MQ=255)


       tttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgc                                                                                          >  2:4331075/1‑101 (MQ=255)


       tttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgc                                                                                          >  2:3842035/1‑101 (MQ=255)


        ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                         >  1:1960861/1‑101 (MQ=255)


         tGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccg                                                                                        >  1:1025657/1‑101 (MQ=255)


           ggTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgcc                                                                                      <  1:1129928/101‑1 (MQ=255)


            gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                     >  1:3525724/1‑101 (MQ=255)


            gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                     >  1:2123536/1‑101 (MQ=255)


             tctcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgc                                                                                    >  2:2768664/1‑101 (MQ=255)


               tcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgccc                                                                                  >  1:325076/1‑100 (MQ=255)


                   aTCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGcc                                                                              >  2:1134582/1‑101 (MQ=255)


                   aTCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGcc                                                                              >  1:3675759/1‑101 (MQ=255)


                   aTCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGcc                                                                              >  1:4085262/1‑101 (MQ=255)


                    tCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGccc                                                                             >  1:1305937/1‑101 (MQ=255)


                     cTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGcccc                                                                            >  2:2024170/1‑101 (MQ=255)


                       tttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcag                                                                          <  2:3547483/101‑1 (MQ=255)


                          ccAGCGAGGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                       <  1:1298927/101‑1 (MQ=255)


                          ccAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                       >  1:2304892/1‑101 (MQ=255)


                          ccAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                       >  2:4137941/1‑101 (MQ=255)


                          ccAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                       >  2:107990/1‑101 (MQ=255)


                           cAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgca                                                                      >  1:944972/1‑101 (MQ=255)


                           cAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgca                                                                      >  1:3153258/1‑101 (MQ=255)


                            aGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcag                                                                     <  2:869544/101‑1 (MQ=255)


                             gCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcagc                                                                    >  2:1823753/1‑101 (MQ=255)


                              cgacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcagcg                                                                   >  1:649307/1‑101 (MQ=255)


                               gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                  >  1:1997324/1‑101 (MQ=255)


                               gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                  >  1:3899423/1‑101 (MQ=255)


                                acgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGcc                                                                 >  1:574305/1‑101 (MQ=255)


                                 cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCt                                                                <  1:3785059/101‑1 (MQ=255)


                                  gaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTc                                                               <  2:2688393/101‑1 (MQ=255)


                                  gaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTc                                                               <  2:1516896/101‑1 (MQ=255)


                                   aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                              <  2:1802331/101‑1 (MQ=255)


                                   aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                              <  2:2222951/101‑1 (MQ=255)


                                    ggCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCgg                                                             <  2:2316174/101‑1 (MQ=255)


                                    ggCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCgg                                                             <  1:2553388/101‑1 (MQ=255)


                                       gcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCa                                                          >  1:1004595/1‑101 (MQ=255)


                                        cgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAg                                                         <  2:2895816/101‑1 (MQ=255)


                                         gcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAgg                                                        >  1:2078382/1‑101 (MQ=255)


                                           aaGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCt                                                      >  1:3186803/1‑101 (MQ=255)


                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                     <  1:4503696/101‑1 (MQ=255)


                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                     >  1:1460480/1‑101 (MQ=255)


                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                     <  2:210574/101‑1 (MQ=255)


                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                     >  2:4173825/1‑101 (MQ=255)


                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                     >  2:637638/1‑101 (MQ=255)


                                             gagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCa                                                    >  1:1546845/1‑101 (MQ=255)


                                              agCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAg                                                   >  2:574876/1‑101 (MQ=255)


                                                 cGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGccc                                                >  2:2194490/1‑101 (MQ=255)


                                                  gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                               >  2:3151433/1‑101 (MQ=255)


                                                  gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                               >  2:3282595/1‑101 (MQ=255)


                                                  gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                               <  1:4321037/101‑1 (MQ=255)


                                                  gTTTCACATCGGCCAGCGTCCAAAGCTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                               <  2:1543327/101‑1 (MQ=255)


                                                    ttCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCCCGGGCAGGCTCAGCCcgcc                                             >  1:831258/1‑100 (MQ=255)


                                                       acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                          >  1:4542566/1‑101 (MQ=255)


                                                        caTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCg                                         <  1:479424/101‑1 (MQ=255)


                                                         aTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGCCATCACCGCCGGCGCCGCCGCCGCCGCGCCCCCGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGa                                        >  2:2470885/1‑101 (MQ=255)


                                                          tggcacAGCGTGCAAAGGTCGGTGCCGGGCGTCCTCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAg                                       <  1:1713494/96‑1 (MQ=255)


                                                          tCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAg                                       <  2:2118418/101‑1 (MQ=255)


                                                           cGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAgg                                      >  1:3472295/1‑101 (MQ=255)


                                                            ggCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGc                                     >  2:1313713/1‑101 (MQ=255)


                                                             gCCAGCGTGCAAAGGTGGGTGGCGGGTGTCACCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCt                                    <  2:106327/101‑1 (MQ=255)


                                                               cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                  <  1:1366408/101‑1 (MQ=255)


                                                               cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                  <  1:1409562/101‑1 (MQ=255)


                                                               cAGCGTCCAAAGGTCGGTTGCGGGGGTCACCACCGCCGCCGCCGCCGCCTCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                  <  1:3870759/101‑1 (MQ=255)


                                                                  cGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAggg                               <  2:1303901/101‑1 (MQ=255)


                                                                   gTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAgggg                              >  2:1611936/1‑100 (MQ=255)


                                                                   gTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGt                              <  2:450155/101‑1 (MQ=255)


                                                                     gCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGa                            >  2:3352393/1‑101 (MQ=255)


                                                                      gaaaGGTCGGTGGCGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCAGCGGCCGAGGCTGAGGGTGaa                           <  1:3490940/100‑1 (MQ=255)


                                                                      cAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGCGGCTGAGGGTGaa                           >  2:2514591/1‑101 (MQ=255)


                                                                      cAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGaa                           >  1:3108009/1‑101 (MQ=255)


                                                                      cAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGGGaa                           >  2:1974059/1‑101 (MQ=255)


                                                                         aGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggc                        >  2:3685925/1‑101 (MQ=255)


                                                                          ggTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcg                       <  2:3630763/101‑1 (MQ=255)


                                                                           gTCGGTGGCGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcgg                      <  1:4167894/101‑1 (MQ=255)


                                                                           gTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcgg                      <  2:304810/101‑1 (MQ=255)


                                                                            gcGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcggc                     <  2:649246/100‑1 (MQ=255)


                                                                             cGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGgcggcg                    >  1:4599122/1‑101 (MQ=255)


                                                                               gTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgac                  <  2:1689457/101‑1 (MQ=255)


                                                                                tGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgacg                 >  2:3231950/1‑101 (MQ=255)


                                                                                tGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGaaa              >  1:2972334/1‑101 (MQ=255)


                                                                                  gCGGGTGTCACCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGaa               <  1:2445498/101‑1 (MQ=255)


                                                                                   cGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGaaa              >  2:1184537/1‑101 (MQ=255)


                                                                                   cGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTg           >  1:4581244/1‑101 (MQ=255)


                                                                                    gggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAAc             >  2:474171/1‑101 (MQ=255)


                                                                                      gtgtCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTg           >  2:3446797/1‑101 (MQ=255)


                                                                                         tcatccCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCg        <  2:2972714/101‑1 (MQ=255)


                                                                                          catccCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGTAGGCGGCGACGAAACTGTCGc       <  1:2645377/101‑1 (MQ=255)


                                                                                          catccCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGc       <  1:1654401/101‑1 (MQ=255)


                                                                                          catcaCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGc       >  1:1771361/1‑101 (MQ=255)


                                                                                          catcaACGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGGCCGCGGCCGAGGCTGAGGGTGAAGGAGGAGACGAAACTGTCGc       >  2:4645635/1‑101 (MQ=255)


                                                                                              cccgccgccgccgccgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcgc   <  2:1831930/100‑1 (MQ=255)


                                                                                              cccgccgccgccgccgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcgc   <  2:3495304/100‑1 (MQ=255)


                                                                                              aCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcgc   >  2:2610750/1‑101 (MQ=255)


                                                                                              aCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGCGAAGGCGGCGACGAAAATGTCGCCcgc   >  1:997475/1‑101 (MQ=255)


                                                                                               ccgccgccgccgccgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcgcg  <  1:2157493/101‑1 (MQ=255)


TCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCG  >  NC_014034/1208341‑1208536

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG