Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,208,438 Δ1 bp 2.5% coding (866/948 nt) pfkB ← 6‑phosphofructokinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,208,4380G→. 2.5% 4.4 0.0 ‑0.7163coding (866/948 nt)pfkB6‑phosphofructokinase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (95/64):  total (97/66)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.06e-01

Only 100 of 228 total aligned reads displayed.

TCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCGCC  >  NC_014034/1208341‑1208538
                                                                                                 |                                                                                                    
  cccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgcc                                                                                                 <  1:3819470/101‑1 (MQ=255)
      gTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccg                                                                                             >  1:106341/1‑101 (MQ=255)
      gTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccg                                                                                             <  1:4397815/101‑1 (MQ=255)
       tttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgc                                                                                            >  2:3490682/1‑101 (MQ=255)
        ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                           >  2:1069264/1‑101 (MQ=255)
        ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                           >  1:1715513/1‑101 (MQ=255)
        ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                           >  1:210598/1‑101 (MQ=255)
        ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                           >  1:1960861/1‑101 (MQ=255)
         tGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccg                                                                                          >  1:1025657/1‑101 (MQ=255)
         tGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccg                                                                                          <  2:1788454/101‑1 (MQ=255)
           ggTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgcc                                                                                        <  1:1129928/101‑1 (MQ=255)
            gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                       >  1:3525724/1‑101 (MQ=255)
            gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                       >  1:2123536/1‑101 (MQ=255)
             tctcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgc                                                                                      >  2:2768664/1‑101 (MQ=255)
              ctcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgcc                                                                                     <  2:2214777/101‑1 (MQ=255)
              ctcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgcc                                                                                     >  2:2656367/1‑101 (MQ=255)
               tcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgccg                                                                                    >  1:2316336/1‑101 (MQ=255)
                  gATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCgcgc                                                                                 >  1:4651132/1‑101 (MQ=255)
                   aTCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGcc                                                                                >  2:1134582/1‑101 (MQ=255)
                   aTCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGcc                                                                                >  1:4085262/1‑101 (MQ=255)
                   aTCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGcc                                                                                >  1:3675759/1‑101 (MQ=255)
                    tCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGccc                                                                               >  1:1305937/1‑101 (MQ=255)
                       tttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcag                                                                            <  2:2235929/101‑1 (MQ=255)
                       tttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcag                                                                            <  2:3547483/101‑1 (MQ=255)
                          ccAGCGAGGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                         <  1:1298927/101‑1 (MQ=255)
                          ccAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                         >  2:4137941/1‑101 (MQ=255)
                           cAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgca                                                                        >  1:944972/1‑101 (MQ=255)
                            aGCGACGAGGGTAGCAAGAGCCTTTTGCCTTCGGCCAGGGTGCAAAGGTCGGTGGCGGGTTTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcag                                                                       <  2:1083994/101‑1 (MQ=255)
                            aGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcag                                                                       <  2:869544/101‑1 (MQ=255)
                            aGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCcagcgcagctc                                                                    <  2:1912769/101‑3 (MQ=255)
                              cgacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcagcg                                                                     <  2:1323170/101‑1 (MQ=255)
                              cgacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcagcg                                                                     >  1:649307/1‑101 (MQ=255)
                               gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                    >  1:1997324/1‑101 (MQ=255)
                               gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                    >  1:3899423/1‑101 (MQ=255)
                                acgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGcc                                                                   >  1:574305/1‑101 (MQ=255)
                                 cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCt                                                                  >  2:2069751/1‑101 (MQ=255)
                                  gaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTc                                                                 <  2:1516896/101‑1 (MQ=255)
                                   aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                                <  2:3073684/101‑1 (MQ=255)
                                   aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                                <  2:1960726/101‑1 (MQ=255)
                                   aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                                <  2:1802331/101‑1 (MQ=255)
                                    ggCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCgg                                                               <  1:2553388/101‑1 (MQ=255)
                                      cgcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGc                                                             <  2:2693895/101‑1 (MQ=255)
                                      cgcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGc                                                             >  2:239069/1‑101 (MQ=255)
                                      cgcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGc                                                             <  1:375855/101‑1 (MQ=255)
                                       gcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCa                                                            >  1:1004595/1‑101 (MQ=255)
                                        cgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAg                                                           <  2:2895816/101‑1 (MQ=255)
                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                       <  1:84311/101‑1 (MQ=255)
                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                       >  2:637638/1‑101 (MQ=255)
                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                       >  1:1460480/1‑101 (MQ=255)
                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                       <  1:4503696/101‑1 (MQ=255)
                                            agagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTc                                                       >  2:4173825/1‑101 (MQ=255)
                                             gagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGctgccttc                                                      <  2:2607283/101‑8 (MQ=255)
                                             gagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCa                                                      >  1:1546845/1‑101 (MQ=255)
                                                 cGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGccc                                                  >  2:1826864/1‑101 (MQ=255)
                                                 cGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGccc                                                  <  2:3409932/101‑1 (MQ=255)
                                                  gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                                 >  2:3151433/1‑101 (MQ=255)
                                                  gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                                 <  1:4321037/101‑1 (MQ=255)
                                                  gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                                 >  2:3282595/1‑101 (MQ=255)
                                                  gTTTCACATCGGCCAGCGTCCAAAGCTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                                 <  2:1543327/101‑1 (MQ=255)
                                                       acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                            <  2:4271384/101‑1 (MQ=255)
                                                       acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                            >  1:4542566/1‑101 (MQ=255)
                                                        caTCGGCCAGCGTGCCAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCg                                           <  2:2107609/101‑1 (MQ=255)
                                                        caTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCg                                           <  1:479424/101‑1 (MQ=255)
                                                        caTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCg                                           >  1:3347679/1‑101 (MQ=255)
                                                         aTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGa                                          >  2:3746294/1‑101 (MQ=255)
                                                           cGGGCAGCGTGCAAAGTTCGGTGTCGGGTGTCATCACCGCCGCCGCCTGCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGTCCGCgg                                        <  1:2666405/101‑1 (MQ=255)
                                                              ccAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTg                                     >  1:2406972/1‑101 (MQ=255)
                                                               cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                    >  1:3156827/1‑101 (MQ=255)
                                                               cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                    <  1:1409562/101‑1 (MQ=255)
                                                               cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                    <  1:1366408/101‑1 (MQ=255)
                                                               cAGCGTCCAAAGGTCGGTTGCGGGGGTCACCACCGCCGCCGCCGCCGCCTCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                    <  1:3870759/101‑1 (MQ=255)
                                                                 gCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAgg                                  >  2:1646226/1‑101 (MQ=255)
                                                                   gTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGt                                <  2:450155/101‑1 (MQ=255)
                                                                   gTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGt                                <  1:3052601/101‑1 (MQ=255)
                                                                     gCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGa                              <  2:2123386/101‑1 (MQ=255)
                                                                      gaaaGGTCGGTGGCGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCAGCGGCCGAGGCTGAGGGTGaa                             <  1:3490940/100‑1 (MQ=255)
                                                                      cAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGaa                             >  1:3108009/1‑101 (MQ=255)
                                                                      cAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGGGaa                             >  2:1974059/1‑101 (MQ=255)
                                                                          ggTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcg                         <  2:3630763/101‑1 (MQ=255)
                                                                           gTCGGTGGCGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcgg                        <  1:4167894/101‑1 (MQ=255)
                                                                            gcGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcggc                       <  2:649246/100‑1 (MQ=255)
                                                                                tGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgacg                   >  2:3231950/1‑101 (MQ=255)
                                                                                  gCGGGTGTCACCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGaa                 <  1:2445498/101‑1 (MQ=255)
                                                                                   cGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGaaa                >  2:1184537/1‑101 (MQ=255)
                                                                                   cGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTg             >  1:4581244/1‑101 (MQ=255)
                                                                                    gggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAAc               >  2:474171/1‑101 (MQ=255)
                                                                                    gggTGTCACCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAAc               <  2:2304730/101‑1 (MQ=255)
                                                                                     ggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACt              >  2:2480203/1‑101 (MQ=255)
                                                                                      gtgccctccccgccgcccccgccgccgccgCCCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTg             <  1:3231737/92‑1 (MQ=255)
                                                                                         tcatccCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCg          <  2:2972714/101‑1 (MQ=255)
                                                                                          catccCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGc         <  1:1654401/101‑1 (MQ=255)
                                                                                          catcaCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGc         <  2:1305844/101‑1 (MQ=255)
                                                                                          catcaCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGc         >  1:1771361/1‑101 (MQ=255)
                                                                                          catcaACGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGGCCGCGGCCGAGGCTGAGGGTGAAGGAGGAGACGAAACTGTCGc         >  2:4645635/1‑101 (MQ=255)
                                                                                             caCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcg      <  2:574257/101‑1 (MQ=255)
                                                                                              aCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcgc     >  2:3646760/1‑101 (MQ=255)
                                                                                              aCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcgc     >  2:3445660/1‑101 (MQ=255)
                                                                                              aCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGCGAAGGCGGCGACGAAAATGTCGCCcgc     >  1:997475/1‑101 (MQ=255)
                                                                                               ccgccgccgccgccgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcgcg    <  1:2157493/101‑1 (MQ=255)
                                                                                                 gccgccgccgccgccgccgcGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCGcc  <  1:346719/101‑1 (MQ=255)
                                                                                                 |                                                                                                    
TCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCGCC  >  NC_014034/1208341‑1208538

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG