Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,831 Δ1 bp 14.4% coding (713/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8310C→.14.4% 107.7 0.0 ‑0.0236coding (713/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/18):  ref base (94/107):  total (111/125)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.94e-01

Only 100 of 248 total aligned reads displayed.

TCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396731‑1396930
                                                                                                          |                                                                                                   
  gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCtt                                                                                                   >  1:1251995/1‑101 (MQ=255)
  gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCtt                                                                                                   <  1:2313668/101‑1 (MQ=255)
   aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTg                                                                                                  >  2:588735/1‑101 (MQ=255)
   aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTg                                                                                                  >  2:2071986/1‑101 (MQ=255)
   aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTg                                                                                                  >  2:4247782/1‑101 (MQ=255)
       ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTc                                                                                              <  2:1764769/101‑1 (MQ=255)
        cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCg                                                                                             <  1:3775379/101‑1 (MQ=255)
          gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCt                                                                                           >  2:4003438/1‑101 (MQ=255)
           tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTc                                                                                          >  1:2611965/1‑101 (MQ=255)
              cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcccc                                                                                       >  1:4646107/1‑101 (MQ=255)
               tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccccc                                                                                      >  1:1940352/1‑101 (MQ=255)
                cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcccccc                                                                                     <  2:1483760/101‑1 (MQ=255)
                 atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGGCG.GCTTGGGTGGCTCCCCCcg                                                                                    >  1:4072288/1‑101 (MQ=255)
                 atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                                    >  2:160747/1‑101 (MQ=255)
                 atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                                    >  2:3561534/1‑101 (MQ=255)
                   atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcgcg                                                                                  >  2:562950/1‑101 (MQ=255)
                   atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcgcg                                                                                  <  2:3237843/101‑1 (MQ=255)
                   atGCTGCCACGATGCCCGTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcgcg                                                                                  <  1:4315041/101‑1 (MQ=255)
                    tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCgg                                                                                 <  1:1116212/101‑1 (MQ=255)
                       tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCggggg                                                                              <  1:3083897/101‑1 (MQ=255)
                          cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCgg                                                                           >  2:1993233/1‑100 (MQ=255)
                          cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGt                                                                           <  2:2589778/101‑1 (MQ=255)
                            cgctGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  1:909212/98‑1 (MQ=255)
                              aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  1:2480959/101‑1 (MQ=255)
                               tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCTGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:396184/101‑1 (MQ=255)
                                gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     >  1:805437/1‑101 (MQ=255)
                                acatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  1:2989080/93‑1 (MQ=2)
                                acatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  2:2871247/93‑1 (MQ=2)
                                  ccTTGAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  1:1548841/101‑1 (MQ=255)
                                   ccgccctCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  1:3285712/95‑1 (MQ=2)
                                   cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:3889691/101‑1 (MQ=255)
                                   cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:284593/101‑1 (MQ=255)
                                    ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAAc                                                                 >  2:1812804/1‑101 (MQ=255)
                                    ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 >  2:3716133/1‑101 (MQ=255)
                                     gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:354658/98‑1 (MQ=2)
                                      gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                               >  2:2112991/1‑101 (MQ=255)
                                      ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGAT....CTCAGTCTGGGTGCGCTCGCGGTTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 >  2:4031131/3‑101 (MQ=2)
                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                >  1:1370549/2‑100 (MQ=2)
                                       cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                >  1:3475209/2‑100 (MQ=2)
                                       aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGGCGCCTCCCCCGCGGGGGCGTGGATCCAAACCgg                                                              >  2:2076235/1‑101 (MQ=37)
                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGCTCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                               <  2:1341982/101‑3 (MQ=2)
                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                               >  1:823039/1‑99 (MQ=2)
                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCGCGGTCGGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                               <  1:535317/101‑3 (MQ=2)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                              >  1:2911402/1‑98 (MQ=2)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                                             >  1:24101/1‑97 (MQ=2)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                                             <  1:4461483/101‑5 (MQ=2)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggc                                                            >  1:3813163/1‑96 (MQ=2)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                                          <  1:1322040/101‑1 (MQ=255)
                                            cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                                           <  1:733724/101‑7 (MQ=2)
                                            cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                                           >  1:2684458/1‑95 (MQ=2)
                                              ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                                         <  2:1164565/101‑9 (MQ=2)
                                              ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                                         <  2:2932864/101‑9 (MQ=2)
                                                ccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatgg                                                       <  1:199595/101‑11 (MQ=0)
                                                ccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                                     <  2:2611780/101‑1 (MQ=255)
                                                 cccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                                    <  1:1477626/101‑1 (MQ=255)
                                                  ccGGGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                                   <  2:3393740/101‑1 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                                 >  1:1890714/1‑101 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                                 <  2:44070/101‑1 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                                >  2:1659363/1‑101 (MQ=255)
                                                      gggggCGTGGATCGAAACCGTGTGGAT...GATTATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                               >  1:4145516/1‑101 (MQ=255)
                                                      gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                               <  1:1018737/101‑1 (MQ=255)
                                                      gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCCGAGATAGAccc                                               >  1:944597/1‑100 (MQ=255)
                                                      gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                               >  2:3851140/1‑101 (MQ=255)
                                                         ggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                                            <  1:2123608/101‑1 (MQ=255)
                                                           cGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                                          <  2:756386/101‑1 (MQ=255)
                                                            gTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                                         >  2:2270850/1‑101 (MQ=255)
                                                             tGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                        >  1:749560/1‑101 (MQ=255)
                                                              ggATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                                       <  1:1812930/101‑1 (MQ=255)
                                                               gATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                                      >  1:2735566/1‑101 (MQ=255)
                                                                aTCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                                     >  1:4192734/1‑101 (MQ=255)
                                                                   gAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                                  >  2:2675261/1‑101 (MQ=255)
                                                                   gAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                                  >  2:1500178/1‑101 (MQ=255)
                                                                    aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                                 >  1:2038048/1‑101 (MQ=255)
                                                                    aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                                 >  2:3078874/1‑101 (MQ=255)
                                                                    aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                                 <  1:255593/101‑1 (MQ=255)
                                                                      aCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa                               <  2:4645806/101‑1 (MQ=255)
                                                                       ccGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                              >  1:1188569/1‑101 (MQ=255)
                                                                       ccGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                              >  2:832736/1‑101 (MQ=255)
                                                                        cGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                             >  2:2445451/1‑101 (MQ=255)
                                                                        cGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                             <  2:3763606/101‑1 (MQ=255)
                                                                           gtgGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                          <  2:1264005/101‑1 (MQ=255)
                                                                           gtgGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                          <  2:3210238/101‑1 (MQ=255)
                                                                            tgGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGc                         >  2:894744/1‑101 (MQ=255)
                                                                              gat...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc                       <  2:1433707/101‑1 (MQ=255)
                                                                              gat...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc                       <  2:4072002/101‑1 (MQ=255)
                                                                                t...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                     <  1:4628105/101‑1 (MQ=255)
                                                                                     atCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccctc                   >  1:1096611/1‑99 (MQ=255)
                                                                                     atCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccc                   >  1:1676259/1‑101 (MQ=255)
                                                                                      tCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc                  <  2:2304326/101‑1 (MQ=255)
                                                                                       cATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccccc                 <  1:3677309/101‑2 (MQ=255)
                                                                                          gC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCgg              >  2:3430531/1‑101 (MQ=255)
                                                                                           c..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggg             <  1:588796/101‑1 (MQ=255)
                                                                                              aGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCgggg            <  2:4593369/101‑1 (MQ=255)
                                                                                                cgcgGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGc          <  1:2238513/101‑1 (MQ=255)
                                                                                                 gcgGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCg         >  2:2633807/1‑101 (MQ=255)
                                                                                                   ggACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTg       >  2:2201751/1‑101 (MQ=255)
                                                                                                   ggACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTg       >  1:1453534/1‑101 (MQ=255)
                                                                                                    gggctgCTTGGCCCGCTCCCCCCGCGGGGGCGTGGATCGAACCCGGTCCAGTGATAGACCGCCCGGAGCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTg       <  2:3616189/96‑1 (MQ=37)
                                                                                                     aCG.GTTTGTGTCGCTCCCCCCGCGGGGGCATCTATCGAAACCGGTCCAGAGATCGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGa     <  1:2256374/101‑1 (MQ=38)
                                                                                                         gCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCg  >  2:1033959/1‑101 (MQ=255)
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TCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396731‑1396930

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG