Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,935 Δ1 bp 11.8% coding (817/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9350A→.11.8% 122.1 ‑0.5 ‑0.4349coding (817/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (23/18):  ref base (144/136):  total (179/170)
Fisher's exact test strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.84e-01

Only 100 of 368 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGT  >  NC_014034/1396837‑1397035
                                                                                                  |                                                                                                               
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.Gcc                                                                                                             <  2:4002012/101‑1 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCt                                                                                                            >  1:756992/1‑101 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCt                                                                                                            <  1:832812/101‑1 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                          <  1:2073734/101‑1 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcg                                                                                                        >  1:2444547/1‑101 (MQ=255)
      cccccGCGGGGGCGTGGATCGAAACCGGTCGAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcgc                                                                                                       >  1:4146222/1‑101 (MQ=255)
      cccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcgc                                                                                                       >  1:2699868/1‑101 (MQ=255)
       ccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.Gcgcg                                                                                                      <  1:1006551/101‑1 (MQ=255)
       ccccGCGGGGGCGTGGATCGAAACCGGCCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.Gcgcg                                                                                                      >  1:563834/1‑101 (MQ=255)
          cgcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.Gc.c                                                                                                 <  1:2921224/101‑1 (MQ=255)
             gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                              >  1:1083970/1‑101 (MQ=255)
              ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                            <  1:1917167/101‑1 (MQ=255)
              ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                            >  1:2483337/1‑101 (MQ=255)
               gggGGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                           <  1:2155617/101‑1 (MQ=255)
                ggCGTGGATCGAGACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                          <  1:2296494/101‑1 (MQ=255)
                ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                          >  2:4602678/1‑101 (MQ=255)
                  cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGc                                                                                        >  2:4062620/1‑101 (MQ=255)
                       aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.Cg                                                                                 <  1:1523402/101‑1 (MQ=255)
                          gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                             >  2:393616/1‑101 (MQ=255)
                           aaaCCGGTCCAGAGATAGCCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTg                                                                            <  1:955847/101‑1 (MQ=255)
                           aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTg                                                                            <  1:187150/101‑1 (MQ=255)
                            aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..c                                                                         <  2:1532053/101‑1 (MQ=255)
                             aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..Cg                                                                        >  2:162702/1‑101 (MQ=255)
                               cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..Cggg                                                                      >  1:627415/1‑99 (MQ=255)
                                ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGCg.g                                                                    >  1:3224278/1‑101 (MQ=255)
                                 gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.Gt                                                                   <  1:2045554/101‑1 (MQ=255)
                                 gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.Gt                                                                   >  2:1231145/1‑101 (MQ=255)
                                  tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTc                                                                  <  1:860655/101‑1 (MQ=255)
                                  tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTc                                                                  <  2:463615/101‑1 (MQ=255)
                                  tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTc                                                                  >  2:2393811/1‑101 (MQ=255)
                                   ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCg                                                                 <  1:3079118/101‑1 (MQ=255)
                                    cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGc                                                                >  2:3595621/1‑101 (MQ=255)
                                      gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTc                                                              >  2:2317944/1‑101 (MQ=255)
                                       agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcc                                                             >  2:2047632/1‑101 (MQ=255)
                                            gtccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCACCCcg                                                        <  2:944517/99‑1 (MQ=255)
                                               cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                     <  2:1442988/101‑1 (MQ=255)
                                                gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                    >  1:1477450/1‑101 (MQ=255)
                                                  gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                  >  2:1950336/1‑101 (MQ=255)
                                                   cgtaCCTCCTCAGACGGCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                 <  2:4145228/98‑1 (MQ=255)
                                                   cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                 >  2:2520896/1‑101 (MQ=255)
                                                    ggccctcctCAGACGTCGCCCCCCCCGCGGGGAGTTGGATCGAAACA.GCCT.GCGCG.G..CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                               <  1:4003723/98‑1 (MQ=38)
                                                     gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                               >  2:2728398/1‑101 (MQ=255)
                                                       cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                             <  2:841411/101‑1 (MQ=255)
                                                         ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:3162509/92‑1 (MQ=2)
                                                         tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:2113141/101‑1 (MQ=255)
                                                         gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                            <  2:3143444/92‑1 (MQ=9)
                                                          tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  1:4417412/9‑101 (MQ=2)
                                                          tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:258215/93‑1 (MQ=9)
                                                          gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgggt                                           >  2:4362149/8‑99 (MQ=0)
                                                          gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:685449/94‑1 (MQ=2)
                                                          gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:1135072/94‑1 (MQ=2)
                                                          gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:1370454/94‑1 (MQ=2)
                                                             gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  2:1265337/5‑101 (MQ=2)
                                                             accctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  1:1937866/96‑1 (MQ=9)
                                                              ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  2:2149569/4‑101 (MQ=2)
                                                              ggtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.AGGGCG.TCTGGA.ACA..GGAT.GTCGCTCCCCCCGCCGGGGCGTGGAtggga                                       >  1:3690788/5‑97 (MQ=0)
                                                              aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  2:4192446/101‑1 (MQ=255)
                                                              aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      >  2:1756737/1‑101 (MQ=255)
                                                               tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGGGTGGATCGaaa                                      >  2:1783993/3‑101 (MQ=2)
                                                               actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      >  1:2267642/4‑101 (MQ=9)
                                                                cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                    >  2:1200031/3‑100 (MQ=2)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCCCAACac                                   >  2:3204231/1‑100 (MQ=2)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                   >  2:2680248/1‑99 (MQ=9)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCGT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                   >  2:764895/1‑99 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                 <  2:2444381/101‑1 (MQ=255)
                                                                   ccccTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                 <  1:1659474/98‑1 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCGCCGCGGGGGCGTGGATCGAAAcccgc                                 >  1:3919459/1‑97 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                  <  1:1346730/101‑3 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               <  2:3673995/101‑7 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               <  1:3008021/101‑7 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               <  2:523761/101‑7 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               >  2:3472282/1‑95 (MQ=2)
                                                                      ccccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                              <  2:4447132/99‑8 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                >  2:1883430/1‑100 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccac                              >  2:1017286/1‑94 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                              >  2:340878/1‑94 (MQ=9)
                                                                       cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                             <  1:4602979/100‑1 (MQ=255)
                                                                       cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                             <  1:1756856/100‑1 (MQ=255)
                                                                        ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                            <  1:4485809/101‑10 (MQ=2)
                                                                         cccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgggg                           >  1:2614198/1‑91 (MQ=2)
                                                                          cccgGCGGGGGCGTGGATCGAAACATGGCTAGGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                           <  2:3250934/101‑11 (MQ=2)
                                                                          ccccGCGGGGGCTTGGATCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTC..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                          <  2:200086/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                             <  2:2927158/101‑5 (MQ=2)
                                                                          ccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                           >  1:421990/1‑91 (MQ=2)
                                                                          ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                           >  1:2507218/1‑91 (MQ=2)
                                                                           cccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                         <  1:3561792/101‑1 (MQ=255)
                                                                             cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                       >  1:378779/1‑101 (MQ=255)
                                                                               cgGGGGGGTGGATCGAAACA.GCCT.GCCCG.GN.CGCA.TCGGCT.TC.CTC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                     <  1:1231237/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                    >  2:2223901/1‑101 (MQ=255)
                                                                                  gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                  <  1:2856567/101‑1 (MQ=255)
                                                                                     cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc               >  2:4261865/1‑101 (MQ=255)
                                                                                     cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc               <  1:2779261/101‑1 (MQ=255)
                                                                                        ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCCGCCGGa            >  2:1108903/1‑101 (MQ=255)
                                                                                        ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa            <  2:3776703/101‑1 (MQ=255)
                                                                                         gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc           <  1:3735938/101‑1 (MQ=255)
                                                                                          aTCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg          <  1:2675446/101‑1 (MQ=255)
                                                                                              aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCTCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt      >  1:4331037/1‑101 (MQ=255)
                                                                                              aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt      >  1:3480855/1‑101 (MQ=255)
                                                                                               aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc     >  2:3023395/1‑101 (MQ=255)
                                                                                                 cA.GCGT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGGGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg   <  2:2985757/101‑1 (MQ=255)
                                                                                                  |                                                                                                               
TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGT  >  NC_014034/1396837‑1397035

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG