Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,420,018 Δ1 bp 1.7% coding (307/1644 nt) RCAP_rcc01323 ← IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,420,0180C→. 1.7% 3.8 ‑0.5 ‑0.0237coding (307/1644 nt)RCAP_rcc01323IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (131/102):  total (134/103)
Fisher's exact test strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.91e-01

Only 100 of 312 total aligned reads displayed.

CGCAGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGAAAGTCTGATGG  >  NC_014034/1419919‑1420118
                                                                                                   |                                                                                                    
cGCAGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcc                                                                                                     >  1:4552769/1‑101 (MQ=255)
 gCAGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcct                                                                                                    >  2:1555162/1‑101 (MQ=255)
 gCAGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcct                                                                                                    >  2:3053448/1‑101 (MQ=255)
  cAGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctc                                                                                                   <  1:1987783/101‑1 (MQ=255)
  cAGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctc                                                                                                   >  2:3081645/1‑101 (MQ=255)
   aGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGTTTCCCTTTCCGGGGCTTCTGctcctcc                                                                                                  >  2:4582424/1‑101 (MQ=255)
   aGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctcc                                                                                                  >  1:123662/1‑101 (MQ=255)
   aGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctcc                                                                                                  <  1:1643636/101‑1 (MQ=255)
      aGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctcctcc                                                                                               >  2:1653435/1‑101 (MQ=255)
       gCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctcctcct                                                                                              >  2:2077415/1‑101 (MQ=255)
        cAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctcctcctc                                                                                             >  1:1667687/1‑101 (MQ=255)
         aGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctcctcctcc                                                                                            >  1:3510047/1‑101 (MQ=255)
            gCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctcctcctcctcc                                                                                         >  1:4189750/1‑101 (MQ=255)
             cGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctcctcctcctcct                                                                                        <  1:396139/101‑1 (MQ=255)
               tttCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGctcctcctcctcctcctcc                                                                                      >  1:2975468/1‑101 (MQ=255)
                  ccTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCg                                                                                   <  2:532501/101‑1 (MQ=255)
                    tCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGAc                                                                                 <  2:2714113/101‑1 (MQ=255)
                     cGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACa                                                                                <  1:4520390/101‑1 (MQ=255)
                     cGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACa                                                                                >  2:2033936/1‑101 (MQ=255)
                      ggggAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACAt                                                                               >  1:1179032/1‑101 (MQ=255)
                      ggggAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACAt                                                                               <  2:2771717/101‑1 (MQ=255)
                        ggAAATGCTTGTACTCCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACAtcg                                                                             <  2:702613/101‑1 (MQ=255)
                           aaTGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACAtcgtcg                                                                          >  1:963571/1‑101 (MQ=255)
                           aaTGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACAtcgtcg                                                                          <  2:1600780/101‑1 (MQ=255)
                            aTGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACAtcgtcgt                                                                         <  1:2128534/101‑1 (MQ=255)
                             tGCTTGTACGCCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACAtcgtcgtc                                                                        <  1:2289223/101‑1 (MQ=255)
                             tGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACAtcgtcgtc                                                                        <  2:4095574/101‑1 (MQ=255)
                               cTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCgg                                                                      <  1:1199779/101‑1 (MQ=255)
                               cTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCgg                                                                      >  1:2456750/1‑101 (MQ=255)
                                ttGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCggg                                                                     <  1:4483512/101‑1 (MQ=255)
                                   tACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCAc                                                                  <  2:2953872/101‑1 (MQ=255)
                                   tACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCAc                                                                  >  1:3804310/1‑101 (MQ=255)
                                   tACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCAc                                                                  >  2:3680614/1‑101 (MQ=255)
                                     ccccTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGGCGGGCCCGt                                                                <  2:399769/99‑1 (MQ=255)
                                      acCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTc                                                               >  2:2927871/1‑101 (MQ=255)
                                      acCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTc                                                               <  2:3296121/101‑1 (MQ=255)
                                       ccTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCa                                                              >  2:582931/1‑101 (MQ=255)
                                        cTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCAt                                                             >  1:1124796/1‑101 (MQ=255)
                                         tCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATc                                                            >  2:2854029/1‑101 (MQ=255)
                                          cGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATcc                                                           >  2:1323802/1‑101 (MQ=255)
                                            gTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCtc                                                         >  1:3001250/1‑101 (MQ=255)
                                            gTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCtc                                                         <  1:2791995/101‑1 (MQ=255)
                                             tCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCtcg                                                        <  2:951492/101‑1 (MQ=255)
                                             tCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCtcg                                                        <  2:1167578/101‑1 (MQ=255)
                                             tCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCtcg                                                        >  1:2607243/1‑101 (MQ=255)
                                              cTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCtcgt                                                       >  1:4105969/1‑101 (MQ=255)
                                              cTTGATGTTCTTTGGTCGCTTCCGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCCCCTCGACATCGTCGTCGGGCACGTCACCCtcgt                                                       >  1:4001288/1‑101 (MQ=255)
                                                 gATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCtcgtcgt                                                    >  1:4219423/1‑101 (MQ=255)
                                                  aTGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCtcgtcgtc                                                   <  2:3435604/101‑1 (MQ=255)
                                                    gTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTcgtcgtcgt                                                 >  1:762431/1‑101 (MQ=255)
                                                     ttCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCgtcgtcgtc                                                >  1:1786578/1‑101 (MQ=255)
                                                     ttCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCgtcgtcgtc                                                <  1:2674301/101‑1 (MQ=255)
                                                     ttCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCgtcgtcgtc                                                >  2:1734516/1‑101 (MQ=255)
                                                     ttCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCgtcgtcgtc                                                <  2:4552470/101‑1 (MQ=255)
                                                       cTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCCCCTCCTCCTCCTCCTCCTCGACACCGTCGTCGGGCACGTCCTCCTCGTCGTCGTCCg                                              <  2:3880033/101‑1 (MQ=255)
                                                       cTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTG...CTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTccagat                                           >  1:1970378/1‑97 (MQ=255)
                                                          tGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTc                                           >  2:2399607/1‑101 (MQ=255)
                                                           ggTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTcc                                          >  1:88615/1‑101 (MQ=255)
                                                            gTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTccc                                         >  1:4305324/1‑101 (MQ=255)
                                                            gTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTccc                                         >  1:2069452/1‑101 (MQ=255)
                                                            gTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTccc                                         <  1:1418937/101‑1 (MQ=255)
                                                             tCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCa                                        >  1:575239/1‑101 (MQ=255)
                                                             tCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCa                                        <  1:3318841/101‑1 (MQ=255)
                                                             tCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCa                                        <  2:2665143/101‑1 (MQ=255)
                                                               gCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAgg                                      <  2:430098/101‑1 (MQ=255)
                                                               gCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAgg                                      >  2:4515147/1‑101 (MQ=255)
                                                                cTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGa                                     <  1:2854245/101‑1 (MQ=255)
                                                                 ttCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGaa                                    >  1:4649872/1‑101 (MQ=255)
                                                                 ttCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGaa                                    <  1:3782640/101‑1 (MQ=255)
                                                                 ttCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGaa                                    >  2:1215905/1‑101 (MQ=255)
                                                                    tGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAAtt                                 <  1:3579861/101‑1 (MQ=255)
                                                                     gtgtCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAAttg                                >  1:3445222/4‑101 (MQ=255)
                                                                      tttCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAAttgt                               >  1:534761/1‑101 (MQ=255)
                                                                      tttCTGCTTCCCTTTCCGGGGCTTCGGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAAttgt                               >  2:3584823/1‑101 (MQ=255)
                                                                       ttCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAAttgtt                              >  1:3661551/1‑101 (MQ=255)
                                                                        tCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAAttgttg                             >  1:2726475/1‑101 (MQ=255)
                                                                        tCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGGCCGCTCCCAGGAAAttgttg                             >  1:2204301/1‑101 (MQ=255)
                                                                          tGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTc                           >  1:640189/1‑101 (MQ=255)
                                                                             ttCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGa                        >  2:4356899/1‑101 (MQ=255)
                                                                             ttCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGa                        <  1:3745889/101‑1 (MQ=255)
                                                                             ttCCCTTTCCGGGGCTTCTG...CTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCt                     >  1:6969/1‑101 (MQ=255)
                                                                                ccTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCt                     <  1:4259280/101‑1 (MQ=255)
                                                                                ccTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCt                     <  1:807013/101‑1 (MQ=255)
                                                                                 cTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTg                    <  2:2570213/101‑1 (MQ=255)
                                                                                    tCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCg                 <  2:4181505/101‑1 (MQ=255)
                                                                                      cGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCa               >  2:450182/1‑101 (MQ=255)
                                                                                       ggggCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAg              >  2:1879815/1‑101 (MQ=255)
                                                                                        gggCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCTTGGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGa             >  2:1085301/1‑101 (MQ=255)
                                                                                        gggCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGa             >  2:3526549/1‑101 (MQ=255)
                                                                                         ggCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGaa            <  1:1268549/101‑1 (MQ=255)
                                                                                         ggCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGaa            >  1:3253288/1‑101 (MQ=255)
                                                                                          gCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGaaa           <  1:3062158/101‑1 (MQ=255)
                                                                                           cTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGAAAg          <  2:3358941/101‑1 (MQ=255)
                                                                                           cTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGAAAg          <  2:762356/101‑1 (MQ=255)
                                                                                            ttCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGAAAGt         >  2:2830168/1‑101 (MQ=255)
                                                                                              cTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGTCGCAGAAAGTCt       <  1:2532106/101‑1 (MQ=255)
                                                                                               tGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGAAAGTCTg      >  2:3158734/1‑101 (MQ=255)
                                                                                                tctcctcctcctcctcctcctcGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGAAAGTCTGa     <  2:1401427/100‑1 (MQ=255)
                                                                                                gcgcctcctcctcctcctcctcGACATCGTCGTCGGGCACGTCATCCTCGTCGGCGTCCGCCCCCAGGAAATTGTTGTCGGAGCTGCCGCAGAAAGTCTGa     <  2:2499704/98‑1 (MQ=255)
                                                                                                  tcctcctcctcctcctcctcGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGAAAGTCTGATg   >  2:4448573/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
CGCAGGAGCAGTGCGTTTCCTCGGGGAAATGCTTGTACACCTCGGTCTTGATGTTCTTTGGTCGCTTCTGTTTCTGCTTCCCTTTCCGGGGCTTCTGCTCCTCCTCCTCCTCCTCCTCGACATCGTCGTCGGGCACGTCATCCTCGTCGTCGTCCGCTCCCAGGAAATTGTTGTCGGAGCTGCCGCAGAAAGTCTGATGG  >  NC_014034/1419919‑1420118

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG