Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,148 Δ1 bp 1.8% intergenic (‑76/+149) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1480A→. 1.8% 3.6 ‑1.0 ‑0.1228intergenic (‑76/+149)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (108/116):  total (111/117)
Fisher's exact test strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.25e-01

Only 100 of 240 total aligned reads displayed.

GCTCCTCGCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTT.CG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCT  >  NC_014034/1696048‑1696248
                                                                                                    |                                                                                                       
gctCCTCGCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGgaga                                                                                                         <  1:268723/101‑1 (MQ=255)
gctCCTCGCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGgaga                                                                                                         >  1:930792/1‑101 (MQ=255)
  tcctcGCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGgagagg                                                                                                       >  2:1620811/1‑99 (MQ=255)
   cctcGCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGACTTTTGCAAGGAGGGGAGAGgg                                                                                                      <  1:2641429/101‑1 (MQ=255)
     tcGCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGc                                                                                                    <  2:1234130/101‑1 (MQ=255)
     tcGCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGc                                                                                                    <  2:4092939/101‑1 (MQ=255)
      cGCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTCAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGcg                                                                                                   >  2:4124228/1‑100 (MQ=255)
       gCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGcgc                                                                                                  >  1:2435742/1‑99 (MQ=255)
        cTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGcgct                                                                                                 >  2:3760468/1‑98 (MQ=255)
        cTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGcgct                                                                                                 <  2:4303435/101‑4 (MQ=255)
           aGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGcgctgcc                                                                                              >  1:2616307/1‑95 (MQ=255)
            gcgcGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGcgctgccc                                                                                             <  1:1670045/101‑8 (MQ=255)
              gcgAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGcgctgcccct                                                                                           >  2:3480385/1‑92 (MQ=255)
                gAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTcc                                                                                       >  2:2799889/1‑100 (MQ=255)
                gAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTcc                                                                                       <  2:3069650/101‑2 (MQ=255)
                  aaTCCATGCGCTCCTCCCAAAGTGCCTGAATTGTTCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTg                                                                                     <  1:4003900/101‑1 (MQ=255)
                  aaTCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTg                                                                                     <  2:3965774/101‑1 (MQ=255)
                      cATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCt                                                                                 >  2:4065387/1‑101 (MQ=255)
                        tGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCttt                                                                               <  1:950639/101‑1 (MQ=255)
                         gcgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.c                                                                             <  1:1632309/101‑1 (MQ=255)
                         gcgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.c                                                                             <  1:1478572/101‑1 (MQ=255)
                          cgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.Cg                                                                            <  2:230411/101‑1 (MQ=255)
                            ctcctcCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.c                                                                        >  1:2212669/1‑101 (MQ=255)
                             tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.cc                                                                       >  1:3046814/1‑100 (MQ=255)
                             tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.cc                                                                       >  2:698925/1‑100 (MQ=255)
                              cctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.cca                                                                      <  1:1731535/101‑3 (MQ=255)
                                tccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaat                                                                    <  1:4293642/101‑5 (MQ=255)
                                 cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaatt                                                                   >  2:1478577/1‑96 (MQ=255)
                                 cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaatt                                                                   <  2:2236730/101‑6 (MQ=255)
                                 cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaatt                                                                   >  2:1369179/1‑96 (MQ=255)
                                  ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTc                                                                   <  1:1674959/101‑1 (MQ=255)
                                  ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTc                                                                   <  1:999257/101‑1 (MQ=255)
                                     aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcc                                                               <  1:2882430/101‑1 (MQ=255)
                                      aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAccc                                                              >  2:1114933/1‑101 (MQ=255)
                                       gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcccc                                                             <  1:2800105/101‑2 (MQ=37)
                                         gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCACCCcgg                                                           >  1:591293/1‑101 (MQ=37)
                                          ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCggg                                                           >  1:1175491/1‑101 (MQ=255)
                                          ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCggg                                                           <  1:1048704/101‑1 (MQ=37)
                                            tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGat                                                         <  2:903628/101‑2 (MQ=255)
                                            tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGat                                                         <  1:452105/101‑2 (MQ=255)
                                             gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatg                                                        <  1:1264316/101‑3 (MQ=25)
                                              aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatgt                                                       >  2:3657744/1‑98 (MQ=255)
                                              aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatgt                                                       <  1:1292656/101‑4 (MQ=255)
                                               aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGAC.CAATTCACCCCGGGatgtt                                                      <  1:2015028/101‑5 (MQ=255)
                                               aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatgtt                                                      >  1:2166922/1‑97 (MQ=25)
                                                ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatgttt                                                     >  2:3155013/1‑96 (MQ=255)
                                                 tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCTAGGAGGGGAG.AGGGACGC.TGCCCCTCCTGGCCTTT.CG.G.CCCATTCACCCCGGGAGGtttg                                                   >  1:1515959/1‑99 (MQ=255)
                                                 tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                    >  2:2979233/1‑101 (MQ=255)
                                                 tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                    <  2:1210245/101‑1 (MQ=255)
                                                 tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                    >  1:551668/1‑101 (MQ=255)
                                                 tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                    >  1:2052590/1‑101 (MQ=255)
                                                  gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCCG.C.CAATTCACCCCGGGacgtttcg                                                   >  1:1089246/1‑94 (MQ=255)
                                                   aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTggg                                                  >  2:451076/1‑101 (MQ=255)
                                                   aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTggg                                                  <  1:1346861/101‑1 (MQ=25)
                                                   aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTggg                                                  >  2:2557420/1‑101 (MQ=255)
                                                      gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCa                                               <  2:960630/101‑1 (MQ=25)
                                                      gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCa                                               <  1:2479879/101‑1 (MQ=25)
                                                       tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGGTGTTTGGGCCaa                                              >  1:673619/1‑101 (MQ=255)
                                                         tGTTTACTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                            <  1:768081/101‑3 (MQ=16)
                                                         tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGGCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                            >  1:4041631/1‑99 (MQ=255)
                                                         tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                            >  1:2314313/1‑99 (MQ=255)
                                                          ttttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCACCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           <  2:730882/100‑4 (MQ=255)
                                                          gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           <  2:2179410/101‑4 (MQ=255)
                                                          gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           >  1:3644898/1‑98 (MQ=255)
                                                          gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           >  1:1884558/1‑98 (MQ=255)
                                                          gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           >  1:176595/1‑98 (MQ=255)
                                                          gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           <  1:962852/101‑4 (MQ=255)
                                                           tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                          <  2:1868248/101‑5 (MQ=255)
                                                                 ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                  <  1:1369274/101‑3 (MQ=255)
                                                                  tCGAATCCTTGCACGAATTTTACAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                 >  1:3439253/1‑98 (MQ=255)
                                                                    gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                               <  2:1704277/101‑6 (MQ=255)
                                                                     aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                              >  1:3626574/1‑95 (MQ=255)
                                                                     aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                              <  2:2664219/101‑7 (MQ=12)
                                                                     aaTCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGGAGTTTGGGCCAAGgggaaacaccttg                                >  1:840174/1‑91 (MQ=255)
                                                                      aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                             <  1:4124514/101‑8 (MQ=255)
                                                                      aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                             >  1:108992/1‑94 (MQ=255)
                                                                       tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                            <  2:2846411/101‑9 (MQ=255)
                                                                        ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                           <  1:4195133/101‑10 (MQ=9)
                                                                        ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGAGGTTTGGGCCAA..GGTGAAacaccttgctt                           >  1:2726372/1‑92 (MQ=255)
                                                                         cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                          <  1:3125239/101‑11 (MQ=255)
                                                                         cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                          >  1:3527130/1‑91 (MQ=255)
                                                                              aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCCATTGACCCCGGGATGTTTGGGCCAA..GGTGAAA.TACCCT.GCTTTTTCtt                   >  2:393602/1‑101 (MQ=255)
                                                                                gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                 <  1:2983853/101‑1 (MQ=255)
                                                                                gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                 <  1:3191089/101‑1 (MQ=255)
                                                                                gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTgg                 >  2:3910996/1‑101 (MQ=9)
                                                                                 aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGAC.CAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                <  2:1667877/101‑1 (MQ=255)
                                                                                 aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                >  1:3861251/1‑101 (MQ=255)
                                                                                  aTTTTGCAAGGAGGGGAG.AGGGGAGC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCCA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg               >  2:1797521/1‑101 (MQ=255)
                                                                                   ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTAGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc              >  2:3575452/1‑101 (MQ=9)
                                                                                    tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA..AACCTTGCTTTTTCTTGGCGCa             >  1:3720776/1‑101 (MQ=255)
                                                                                     ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCCATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa            >  1:4403641/1‑101 (MQ=255)
                                                                                      tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..NGNNNAA.CA.CCTTGNTTTTTCTTGGGGCaaa           >  1:4905/1‑101 (MQ=2)
                                                                                       gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt          <  2:3814493/101‑1 (MQ=9)
                                                                                       gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt          <  1:3789785/101‑1 (MQ=9)
                                                                                       gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt          >  2:4629246/1‑101 (MQ=255)
                                                                                         aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc        <  1:4078852/101‑1 (MQ=255)
                                                                                         aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc        <  2:101686/101‑1 (MQ=255)
                                                                                         aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc        >  2:4253141/1‑101 (MQ=9)
                                                                                             aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct    <  1:4253429/101‑1 (MQ=9)
                                                                                               gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct  <  2:4413375/101‑1 (MQ=255)
                                                                                                    |                                                                                                       
GCTCCTCGCTCAGCGCGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTT.CG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCT  >  NC_014034/1696048‑1696248

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG