BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,696,157	Δ1 bp	100%	intergenic (‑85/+140)	RCAP_rcc01564 ← / ← mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,696,157	0	T→.	100.0%	796.1	198	intergenic (‑85/+140)	RCAP_rcc01564/mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): new base (96/102): ref base (0/0): total (96/102)

Only 100 of 201 total aligned reads displayed.


CGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTT.CG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCT  >  NC_014034/1696063‑1696248


cgAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTc                                                                                        <  1:3703069/101‑1 (MQ=255)


 gAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTcc                                                                                       <  2:3069650/101‑2 (MQ=255)


 gAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTcc                                                                                       >  2:1087902/1‑100 (MQ=255)


 gAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTcc                                                                                       <  1:1826392/101‑2 (MQ=255)


  aaaTCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTcct                                                                                      >  1:3731845/1‑99 (MQ=255)


   aaTCCATGCGCTCCTCCCAAAGTGCCTGAATTGTTCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTg                                                                                     <  1:4003900/101‑1 (MQ=255)


   aaTCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTg                                                                                     <  2:3965774/101‑1 (MQ=255)


   aaTCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTg                                                                                     <  2:1855093/101‑1 (MQ=255)


      ccATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGcc                                                                                  >  2:2774892/1‑101 (MQ=255)


      ccATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGcc                                                                                  >  1:4084552/1‑101 (MQ=255)


       cATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCt                                                                                 <  1:2696755/101‑1 (MQ=255)


       cATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCt                                                                                 >  2:2841458/1‑101 (MQ=255)


          gcgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.c                                                                             <  1:1632309/101‑1 (MQ=255)


          gcgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.c                                                                             <  1:2064020/101‑1 (MQ=255)


           cgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.Cg                                                                            <  1:34704/101‑1 (MQ=255)


           cgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.Cg                                                                            <  2:230411/101‑1 (MQ=255)


            gcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.cag                                                                           >  2:1291567/1‑99 (MQ=255)


             ctcctcCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.c                                                                        >  1:2212669/1‑101 (MQ=255)


              tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.cc                                                                       >  2:698925/1‑100 (MQ=255)


               cctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.cca                                                                      <  1:1731535/101‑3 (MQ=255)


               cctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.cca                                                                      >  2:2861805/1‑99 (MQ=255)


                ctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaa                                                                     <  2:930712/101‑4 (MQ=255)


                  cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaatt                                                                   <  2:2236730/101‑6 (MQ=255)


                  cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaatt                                                                   >  2:1369179/1‑96 (MQ=255)


                  cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaatt                                                                   >  2:1478577/1‑96 (MQ=255)


                   ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTc                                                                   <  1:999257/101‑1 (MQ=255)


                   ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTc                                                                   >  1:552563/1‑101 (MQ=255)


                   ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTc                                                                   <  1:1674959/101‑1 (MQ=255)


                      aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcc                                                               >  1:1269656/1‑101 (MQ=255)


                      aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcc                                                               <  1:2882430/101‑1 (MQ=255)


                        gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcccc                                                             <  1:2800105/101‑2 (MQ=37)


                         tGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAccccg                                                            <  2:3007042/101‑3 (MQ=255)


                           ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCggg                                                           <  1:1048704/101‑1 (MQ=37)


                             tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGat                                                         <  1:452105/101‑2 (MQ=255)


                              gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGata                                                        >  2:452068/1‑99 (MQ=255)


                               aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatgt                                                       <  1:1292656/101‑4 (MQ=255)


                                aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatgtt                                                      >  1:2664404/1‑97 (MQ=25)


                                aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatgtt                                                      >  1:2166922/1‑97 (MQ=25)


                                  tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                    <  2:1210245/101‑1 (MQ=255)


                                  tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                    >  2:4194845/1‑101 (MQ=255)


                                  tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                    <  2:402382/101‑1 (MQ=255)


                                   gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCCG.C.CAATTCACCCCGGGacgtttcg                                                   >  1:1089246/1‑94 (MQ=255)


                                   gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTgg                                                   >  2:4292770/1‑101 (MQ=25)


                                    aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTggg                                                  <  1:1346861/101‑1 (MQ=25)


                                      cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGcc                                                >  2:379954/1‑101 (MQ=25)


                                       gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCa                                               >  1:978310/1‑101 (MQ=255)


                                        tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaa                                              <  2:251361/101‑1 (MQ=255)


                                        tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaa                                              <  1:2451025/101‑1 (MQ=255)


                                         cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                             >  1:1508755/1‑100 (MQ=21)


                                          tGTTTACTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                            <  1:768081/101‑3 (MQ=16)


                                          tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                            >  1:2314313/1‑99 (MQ=255)


                                           ttttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCACCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           <  2:730882/100‑4 (MQ=255)


                                           gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           >  1:176595/1‑98 (MQ=255)


                                           gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           <  2:3871213/101‑4 (MQ=255)


                                           gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                           <  2:2179410/101‑4 (MQ=255)


                                              tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                        >  1:2022913/1‑95 (MQ=255)


                                              tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                        >  2:3708085/1‑95 (MQ=255)


                                                aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                    >  1:1397737/1‑101 (MQ=255)


                                                aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                    <  2:891629/101‑1 (MQ=16)


                                                 tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                   <  1:33938/101‑2 (MQ=255)


                                                  ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                  <  1:1369274/101‑3 (MQ=255)


                                                   tCGAATCCTTGCACTAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                 <  2:3180335/101‑4 (MQ=12)


                                                    cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                >  2:3486355/1‑97 (MQ=255)


                                                    cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                <  2:176571/101‑5 (MQ=255)


                                                     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                               <  1:1115022/101‑6 (MQ=255)


                                                     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                               <  2:1704277/101‑6 (MQ=255)


                                                     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                               <  1:301854/101‑6 (MQ=255)


                                                      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                              >  1:3626574/1‑95 (MQ=255)


                                                      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                              <  1:67973/101‑7 (MQ=12)


                                                      aaTCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGGAGTTTGGGCCAAGgggaaacaccttg                                >  1:840174/1‑91 (MQ=255)


                                                       aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                             >  1:108992/1‑94 (MQ=255)


                                                       aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                             <  1:4124514/101‑8 (MQ=255)


                                                         ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                           <  1:939666/101‑10 (MQ=9)


                                                         ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                           <  1:4195133/101‑10 (MQ=9)


                                                          cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                          <  1:3125239/101‑11 (MQ=255)


                                                          cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                          >  1:4233275/1‑91 (MQ=255)


                                                             gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                     >  2:3648841/1‑101 (MQ=255)


                                                               aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCCATTGACCCCGGGATGTTTGGGCCAA..GGTGAAA.TACCCT.GCTTTTTCtt                   >  2:393602/1‑101 (MQ=255)


                                                               aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                   >  1:516253/1‑101 (MQ=255)


                                                               aCGAATTGTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                   <  2:2022773/101‑1 (MQ=2)


                                                                cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                  <  1:4065673/101‑1 (MQ=255)


                                                                 gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                 <  1:2983853/101‑1 (MQ=255)


                                                                 gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                 <  1:3191089/101‑1 (MQ=255)


                                                                 gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTgg                 >  2:3910996/1‑101 (MQ=9)


                                                                  aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGAC.CAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                <  2:1667877/101‑1 (MQ=255)


                                                                  aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                <  1:2491666/101‑1 (MQ=9)


                                                                  aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                >  1:3861251/1‑101 (MQ=255)


                                                                   aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg               <  1:951987/101‑1 (MQ=255)


                                                                    ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc              >  1:1889287/1‑101 (MQ=9)


                                                                      ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCCATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa            >  1:4403641/1‑101 (MQ=255)


                                                                       tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..NGNNNAA.CA.CCTTGNTTTTTCTTGGGGCaaa           >  1:4905/1‑101 (MQ=2)


                                                                        gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt          >  2:4629246/1‑101 (MQ=255)


                                                                        gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt          <  1:3789785/101‑1 (MQ=9)


                                                                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa         >  1:628172/1‑101 (MQ=255)


                                                                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa         <  1:1547415/101‑1 (MQ=255)


                                                                          aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc        <  2:2614232/101‑1 (MQ=255)


                                                                            ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc      <  2:4429655/101‑1 (MQ=255)


                                                                             gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc     <  1:1213397/101‑1 (MQ=255)


                                                                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct  <  2:4399582/101‑1 (MQ=255)


                                                                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct  <  1:4293058/101‑1 (MQ=255)


CGAAATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTT.CG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCT  >  NC_014034/1696063‑1696248

Base quality scores:


ATCG

< 3 ≤


ATCG

< 21 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG